环状RNA的产生、研究方法及功能
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摘要
随着高通量测序技术的发展,环状RNA(circular RNAs,circRNAs)逐渐成为非编码RNA研究领域的热点。本文系统综述了环状RNA侧翼内含子自身互补配对驱动、RNA结合蛋白驱动以及套索驱动这3种环状RNA形成模型,并从高通量文库构建、生物信息学鉴别和常用的实验验证等3个方面对环状RNA的研究方法进行了介绍。同时,本文详细归纳了环状RNA作为microRNA(miRNA)或蛋白的海绵体、调控宿主基因的选择性剪接和表达、翻译成多肽等多种功能。最后通过系统综述植物环状RNA的特征及最新研究进展,为环状RNA在植物学中的进一步研究提供了新的视野。
The field of circular non-coding RNAs have been gradually attracted wide attention with the developments of high-throughput sequencing. In this review, we systematically summarize three driving models for circRNAs biogenesis:intron-pairing-driven, RNA binding protein-driven and lariat-driven. In addition, we also briefly introduce the current research methods of circRNAs, which include high-throughput library construction methods, identification through bioinformatics and common experimental verification. Here, we also systematically summarize the functions of circRNAs,including microRNA(miRNA) or protein sponges, regulating the alternative splicing(AS) and expression of host genes,and extensive translation. Finally, we provide a systematic characterization and the latest research progress of circRNAs,which provide a new perspective for further studies of circRNAs in plants.
The field of circular non-coding RNAs have been gradually attracted wide attention with the developments of high-throughput sequencing. In this review, we systematically summarize three driving models for circRNAs biogenesis:intron-pairing-driven, RNA binding protein-driven and lariat-driven. In addition, we also briefly introduce the current research methods of circRNAs, which include high-throughput library construction methods, identification through bioinformatics and common experimental verification. Here, we also systematically summarize the functions of circRNAs,including microRNA(miRNA) or protein sponges, regulating the alternative splicing(AS) and expression of host genes,and extensive translation. Finally, we provide a systematic characterization and the latest research progress of circRNAs,which provide a new perspective for further studies of circRNAs in plants.
引文
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