食管鳞状细胞癌组织中HSP27蛋白表达与患者临床病理参数相关性研究
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摘要
目的探讨HSP27蛋白在食管鳞状细胞癌组织中的表达及临床意义。方法应用Envision免疫组织化学染色方法检测食管鳞状细胞癌及癌旁组织中HSP27表达情况。结果 HSP27阳性表达主要定位于细胞质,其在食管鳞状细胞癌组织中的表达水平明显高于癌旁组织(P<0.05)。HSP27阳性表达与T分期和病理分级有关(P<0.05);但与患者性别、年龄、肿瘤大小、病理分型以及淋巴结转移无关(P>0.05)。结论 HSP27阳性表达情况与食管鳞状细胞癌T分期和病理分级有关,提示HSP27可能作为判断食管癌预后以及治疗的新指标。
Objective To analyze the significance of HSP27 protein expression in patients with esophageal squamous cell carcinoma. Methods The expression of HSP27 in esophageal squamous cell carcinoma and adjacent tissues was detected by envision immunohistochemical staining. Results The positive expression of HSP27 was mainly located in the cytoplasm of subcellular structure, and its expression level in esophageal squamous cell carcinoma was significantly higher than that in adjacent tissues(P<0.05). The positive expression of HSP27 was significantly correlated with T stage and pathological grade(P<0.05), but not correlated with gender, age, tumor size, pathological type and lymph node metastasis(P>0.05). Conclusion The positive expression of HSP27 was positively correlated with the T stage and pathological grade of esophageal squamous cell carcinoma, suggesting that HSP27 is involved in the development of esophageal squamous cell carcinoma, which may become a potential new target for the diagnosis, prognosis and treatment of esophageal cancer.
Objective To analyze the significance of HSP27 protein expression in patients with esophageal squamous cell carcinoma. Methods The expression of HSP27 in esophageal squamous cell carcinoma and adjacent tissues was detected by envision immunohistochemical staining. Results The positive expression of HSP27 was mainly located in the cytoplasm of subcellular structure, and its expression level in esophageal squamous cell carcinoma was significantly higher than that in adjacent tissues(P<0.05). The positive expression of HSP27 was significantly correlated with T stage and pathological grade(P<0.05), but not correlated with gender, age, tumor size, pathological type and lymph node metastasis(P>0.05). Conclusion The positive expression of HSP27 was positively correlated with the T stage and pathological grade of esophageal squamous cell carcinoma, suggesting that HSP27 is involved in the development of esophageal squamous cell carcinoma, which may become a potential new target for the diagnosis, prognosis and treatment of esophageal cancer.
引文
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[7] SOLEIMANI A, JALILI-NIK M, AVAN A, et al. The role of HSP27 in the development of drug resistance of gastrointestinal malignancies:Current status and perspectives[J].2019,234(6):8241-8248.
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[10] LIANG H H, HAUNG C Y,CHOU C W, et al. Heat shock protein 27 influences the anti-cancer effect of curcumin in colon cancer cells through ROS production and autophagy activation[J].Life Sciences,2018,209:43-51.
[11] STOPE M B, KLINMMANN G, DIESING K, et al. Heat shock protein HSP27 secretion by ovarian cancer cells is linked to intracellular expression levels,occurs independently of the endoplasmic reticulum pathway and HSP27′s phosphorylation status,and is mediated by exosome liberation[J].Dis Markers,2017,2017:1575374.
[12] ZHANG Y, TAO X, JIN G, et al. A targetable molecular chaperone HSP27 confers aggressiveness in hepatocellular carcinoma[J].Theranostics,2016,6(4):558-570.
[2] ZHANG S, VUITTON L, SHEYHIDIN I, et al. Northwestern China:a place to learn more on oesophageal cancer.Part one:behavioural and environmental risk factors[J].Gastroenterol Hepatol,2010,22(8):917-925.
[3] HAN L, JIANG Y, HAN D, et al. HSP27 regulates epithelial mesenchymal transition, metastasis and proliferation in colorectal carcinoma[J].Oncol Lett,2018,16(4):5309-5316.
[4] ZHOU F, HUANG X, PAN Y, et al. Resveratrol protects HaCaT cells from ultraviolet B-induced photoaging via upregulation of HSP27 and modulation of mitochondrial caspase-dependent apoptotic pathway[J].Biochem Biophys Res Commun,2018,499(3):662-668.
[5] KAWANM M, KAINO S, AMANO S, et al. Heat shock protein 27 expression in EUS-FNA samples can predict gemcitabine sensitivity in pancreatic cancer[J].In Vivo,2018,32(3):637-642.
[6] 赵志华,田研,陈奎生.多发性骨髓瘤中c-myc和MDM2蛋白的表达及其临床意义[J].临床与实验病理学杂志,2017,33(2):165-168.
[7] SOLEIMANI A, JALILI-NIK M, AVAN A, et al. The role of HSP27 in the development of drug resistance of gastrointestinal malignancies:Current status and perspectives[J].2019,234(6):8241-8248.
[8] ZININGA T, RAMATSUI L, SHONHAI A. Heat shock proteins as immunomodulants[J].Molecules,2018,23(11):2846.
[9] CHRISTIAN B, JORDI V, VALENTINA B T, et al. Dependence of HSP27 cellular level on protein kinase CK2 discloses novel therapeutic strategies[J].BBA-General Subjects,2018,1862(12):2902-2910.
[10] LIANG H H, HAUNG C Y,CHOU C W, et al. Heat shock protein 27 influences the anti-cancer effect of curcumin in colon cancer cells through ROS production and autophagy activation[J].Life Sciences,2018,209:43-51.
[11] STOPE M B, KLINMMANN G, DIESING K, et al. Heat shock protein HSP27 secretion by ovarian cancer cells is linked to intracellular expression levels,occurs independently of the endoplasmic reticulum pathway and HSP27′s phosphorylation status,and is mediated by exosome liberation[J].Dis Markers,2017,2017:1575374.
[12] ZHANG Y, TAO X, JIN G, et al. A targetable molecular chaperone HSP27 confers aggressiveness in hepatocellular carcinoma[J].Theranostics,2016,6(4):558-570.