“益培灵”在自由人槭'秋焰'组培快繁中的抑菌效果试验
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摘要
将自由人槭'秋焰'不定芽苗置于MS改良培养基中,分别添加不同浓度的青霉素和"益培灵",培养7 d和21 d后调查瓶苗污染率,结果表明,添加20 mg L~(-1)"益培灵"效果最好,培养21 d后污染率在4.5%。
The adventitious buds of Acer×freemanii 'Autumn Blaze' were placed in MS modified medium, and different concentrations of penicillin and "Yipeiling" were added. After 7 d and 21 d, the contamination rate of the bottle was investigated. Result shows that the addition of 20 mg L~(-1)"Yipeiling" is the optimal, and the contamination rate is 4.5% after 21 days of culture.
The adventitious buds of Acer×freemanii 'Autumn Blaze' were placed in MS modified medium, and different concentrations of penicillin and "Yipeiling" were added. After 7 d and 21 d, the contamination rate of the bottle was investigated. Result shows that the addition of 20 mg L~(-1)"Yipeiling" is the optimal, and the contamination rate is 4.5% after 21 days of culture.
引文
[1] 施隆文,汪霞,卢佳.“益培灵”的抑菌效果及对大花蕙兰类原球茎增殖与分化的影响[J].北方园艺,2002(4):123-125
[2] Eva Wilhelm. Micro propagation of juvenile sycamore maple via adventitious shoot formation by use of thidiazuron[J]. Plant Cell, Tissue and Organ Culture,1999(57): 57-60
[3] Jeffery K Iles, Anna M. Landscape tree cultivar preferences in Iowa[J].U.S. Vold. Journal of Arboriculture,2003,29(6): 331-336
[4] Louis B Anella, Thomas H Whitlow. Flood-tolerance ranking of red and freeman maple cultivars[J]. Journal of Arboriculture,1999,25 (1):31-37
[5] 田郎,谢发成,郑炎诚,等.香蕉试管苗生产的成本分析及其控制对策[J].热带作物科技,1998,23(2): 44-46
[6] 周俊辉,杨妙贤,李春霞,等.在培养基中加入抗生素防止万年青茎段培养污染的研究[J]. 广西植物,2005,25( 3) :233-235
[7] 黄小荣,杨开太.香水白掌的组织培养[J]..广西林业科学,2001,30(1) : 39-40
[8] 周俊辉.植物快速繁殖技术中存在的问题与对策[J].仲恺农业技术学院学报,1999,12( 4) : 64-70
[9] Yong P M, Hutchins A S &Canfield M L .Use of antibiotics to control bacteria in shoot cultures of woody plants[J]. Plant Science Letters, 1984(34):203-209
[2] Eva Wilhelm. Micro propagation of juvenile sycamore maple via adventitious shoot formation by use of thidiazuron[J]. Plant Cell, Tissue and Organ Culture,1999(57): 57-60
[3] Jeffery K Iles, Anna M. Landscape tree cultivar preferences in Iowa[J].U.S. Vold. Journal of Arboriculture,2003,29(6): 331-336
[4] Louis B Anella, Thomas H Whitlow. Flood-tolerance ranking of red and freeman maple cultivars[J]. Journal of Arboriculture,1999,25 (1):31-37
[5] 田郎,谢发成,郑炎诚,等.香蕉试管苗生产的成本分析及其控制对策[J].热带作物科技,1998,23(2): 44-46
[6] 周俊辉,杨妙贤,李春霞,等.在培养基中加入抗生素防止万年青茎段培养污染的研究[J]. 广西植物,2005,25( 3) :233-235
[7] 黄小荣,杨开太.香水白掌的组织培养[J]..广西林业科学,2001,30(1) : 39-40
[8] 周俊辉.植物快速繁殖技术中存在的问题与对策[J].仲恺农业技术学院学报,1999,12( 4) : 64-70
[9] Yong P M, Hutchins A S &Canfield M L .Use of antibiotics to control bacteria in shoot cultures of woody plants[J]. Plant Science Letters, 1984(34):203-209