不同产地油茶果壳多糖含量的研究
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摘要
目的建立油茶果壳多糖质量分数测定方法,研究产地对油茶果壳多糖质量分数的影响。方法采用水提醇沉法制备不同产地油茶果壳多糖,以苯酚-硫酸法结合紫外-可见分光光度法测定其质量分数。结果不同产地油茶果壳多糖质量分数分别为:广东龙川1.96%、广西三江1.75%、江西上犹2.19%、浙江开化1.50%、湖南平江1.70%、湖北麻城1.33%、贵州黎平1.71%、云南腾冲1.78%、四川荣县1.50%、福建尤溪1.67%、安徽宿松1.42%、江苏常山1.36%、重庆1.54%。结论不同产地油茶果壳中多糖质量分数存在一定差异,其中以江西上犹的质量分数最高,湖北麻城的最低。
Objective To establish a determination method of polysaccharides in fruit shell of Camellia oleifera Abe,and investigate the effect of different habitats on the content of polysaccharides. Methods Water extraction and alcohol precipitation method was adopted to prepare polysaccharides in fruit shell of Camellia oleifera Abe from different habitats,and phenol-sulfuric acid method and UV spectrophotometry were used to determine the contents. Results The polysaccharide contents from different habitats were as follows: Longchuan Guangdong 1.96%,Sanjiang Guangxi 1.75%,Shangyou Jiangxi 2.19%,Kaihua Zhejiang1.50%,Pingjiang Hunan 1.70%,Macheng Hubei 1.33%,Liping Guizhou 1.71%,Tengchong Yunnan 1.78%,Rongxiang Sichuan 1. 50%,Youxi Fujian 1. 67%,Susong Anhui 1. 42%,Changshan Jiangsu 1. 36% and Chongqing 1.54%. Conclusion There is great difference in polysaccharide contents in Camellia oleifera Abe from different habitats,the content of total polysaccharides from Shangyou Jiangxi is the highest,while the content of total polysaccharides from Macheng Hubei is the lowest.
Objective To establish a determination method of polysaccharides in fruit shell of Camellia oleifera Abe,and investigate the effect of different habitats on the content of polysaccharides. Methods Water extraction and alcohol precipitation method was adopted to prepare polysaccharides in fruit shell of Camellia oleifera Abe from different habitats,and phenol-sulfuric acid method and UV spectrophotometry were used to determine the contents. Results The polysaccharide contents from different habitats were as follows: Longchuan Guangdong 1.96%,Sanjiang Guangxi 1.75%,Shangyou Jiangxi 2.19%,Kaihua Zhejiang1.50%,Pingjiang Hunan 1.70%,Macheng Hubei 1.33%,Liping Guizhou 1.71%,Tengchong Yunnan 1.78%,Rongxiang Sichuan 1. 50%,Youxi Fujian 1. 67%,Susong Anhui 1. 42%,Changshan Jiangsu 1. 36% and Chongqing 1.54%. Conclusion There is great difference in polysaccharide contents in Camellia oleifera Abe from different habitats,the content of total polysaccharides from Shangyou Jiangxi is the highest,while the content of total polysaccharides from Macheng Hubei is the lowest.
引文
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[11]周勇,易延逵,杨晓敏,等.香菇中多糖含量测定方法的比较研究[J].食品研究与开发,2016,37(13):124-128.
[2]彭开元,胡进波,陈桂华,等.油茶果壳化学成分与燃烧性能分析[J].中国林业科技大学学报,2016,36(7):123-128.
[3]刘潇潇,张丽丹,李华,等.紫外-可见分光光度法测定茶枯多糖的含量[J].今日药学,2016,26(6):384-385,396.
[4]覃佐东,谢吉勇,黄生辉,等.油茶壳综合利用研究进展[J].生物加工过程,2016,14(5):74-78.
[5]范康福,吴雪辉.油茶果壳制备活性炭的工艺研究[J].现代食品科技,2013,29(2):339-341.
[6]朱彬,钟海雁,曹清明,等.油茶活性成分研究进展与展望[J].经济林研究,2010,28(3):140-145.
[7]康海权,陈亚琪,陈秋平,等.油茶果壳多糖的抗氧化作用及单糖组成[J].食品与发酵工业,2010,36(4):36-39.
[8]JIN Xianchun.Bioactivities of water-soluble polysaccharides from fruit shell of Camellia oleifera Abel:antitumor and antioxidant activities[J].Carbohydr Polym,2012,87(3):2198-2201.
[9]WANG Yuefei,WANG Jie,WU Jing,et al.In vitro antioxidant activity and potential inhibitory action againstα-glucosidase of polysaccharides from fruit peel of tea(Camellia sinensis L.)[J].J Zhejiang University Science B:Biomed Biotechnol,2014,15(2):173-180.
[10]廖献就,覃凤英,蒙娴,等.凌云白毫茶多糖的提取和含量测定[J].广东化工,2016,43(24):33-34.
[11]周勇,易延逵,杨晓敏,等.香菇中多糖含量测定方法的比较研究[J].食品研究与开发,2016,37(13):124-128.