改良桃胶多糖的吸湿保湿性能及体外透皮吸收研究
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摘要
【目的】研究改良桃胶多糖的吸湿保湿性能和体外透皮吸收效果,为其进一步应用提供实验依据。【方法】(1)采用干燥器控制湿度的方法,以海藻酸钠和丙三醇为对照,分别在相对湿度(RH)为43%、60%和81%条件下测定改良桃胶多糖的吸湿率,同时在RH 34%和干硅胶环境下测定其保湿率。(2)以大鼠离体皮肤为微渗透屏障,采用Franz扩散池装置进行体外透皮实验,应用苯酚—硫酸法结合紫外—可见分光光度法测定接收池中改良桃胶多糖累积渗透量。【结果】(1)改良桃胶多糖在RH 43%、RH 60%和RH 81%条件下48 h的吸湿率分别为54.5%、67.3%、81.5%,比海藻酸钠高、丙三醇低;而在RH 34%和干硅胶环境中的保湿率为28.7%、26.5%,比海藻酸钠、丙三醇高。(2)改良桃胶多糖的10 g/L、50 g/L质量浓度胶液的稳态透皮速率(JS)分别为1.297、0.688μg·cm~(-2)·h~(-1),透过率分别为5.44%、0.59%,二者相比具有显著性差异(P<0.01)。【结论】改良桃胶多糖具有良好的吸湿、保湿性能;同时具有较好的经皮渗透性能,且质量浓度越低,渗透速度越快。
Objective To study the properties of moisture-absorption and moisture-retention and in-vitrotransdermal permeability of improved peach gum polysaccharose, so as to provide evidence for its furtherapplication. Methods(1)With sodium alginate and glycerol as reference substances,we determined the moisture-absorption rate of the improved peach gum polysaccharose at relative humidity(RH)of 43%,60% and 81%,anddetected the moisture-retention rate of the improved peach gum polysaccharose at RH of 34% and in silica gel bythe method of controlling humidity with the disiccator.(2)Using the isolated rat skin as the micro-transdermalbarrier,we carried out in-vitro transdermal permeation experiment with the instrument of Franz diffusion cells,and measured the cumulative permeation amount of the improved peach gum polysaccharose by phenol-sulfuricacid method combined with ultraviolet spectrophotometry. Results(1)The 48-h moisture-absorption rate of theimproved peach gum polysaccharose at RH of 43%,60%,81% was 54.5%,67.3%,81.5%,respectively,being higher than that of the sodium alginate and lower than that of the glycerol. The moisture-retention rate of theimproved peach gum polysaccharose at RH 34% and in dry silica gel was 28.7%,26.5%,respectively,beinghigher than that of sodium alginate and glycerol.(2)The steady-state penetration rate(JS)of 10,50 g/L of glue solution of polysaccharides from the improved peach gum was 1.297,0.688 μg·cm~(-2)·h~(-1), and the transmittancerate was 5.44%,0.59%,respectively,there being significantly differences between the two mass concentrationgroups(P<0.01). Conclusion The improved peach gum polysaccharose has good properties of moisture-absorptionand moisture-retention, and also has better transdermal penetration and absorption properties, with the lowertheir mass concentrations,the faster transdermal permeation.
Objective To study the properties of moisture-absorption and moisture-retention and in-vitrotransdermal permeability of improved peach gum polysaccharose, so as to provide evidence for its furtherapplication. Methods(1)With sodium alginate and glycerol as reference substances,we determined the moisture-absorption rate of the improved peach gum polysaccharose at relative humidity(RH)of 43%,60% and 81%,anddetected the moisture-retention rate of the improved peach gum polysaccharose at RH of 34% and in silica gel bythe method of controlling humidity with the disiccator.(2)Using the isolated rat skin as the micro-transdermalbarrier,we carried out in-vitro transdermal permeation experiment with the instrument of Franz diffusion cells,and measured the cumulative permeation amount of the improved peach gum polysaccharose by phenol-sulfuricacid method combined with ultraviolet spectrophotometry. Results(1)The 48-h moisture-absorption rate of theimproved peach gum polysaccharose at RH of 43%,60%,81% was 54.5%,67.3%,81.5%,respectively,being higher than that of the sodium alginate and lower than that of the glycerol. The moisture-retention rate of theimproved peach gum polysaccharose at RH 34% and in dry silica gel was 28.7%,26.5%,respectively,beinghigher than that of sodium alginate and glycerol.(2)The steady-state penetration rate(JS)of 10,50 g/L of glue solution of polysaccharides from the improved peach gum was 1.297,0.688 μg·cm~(-2)·h~(-1), and the transmittancerate was 5.44%,0.59%,respectively,there being significantly differences between the two mass concentrationgroups(P<0.01). Conclusion The improved peach gum polysaccharose has good properties of moisture-absorptionand moisture-retention, and also has better transdermal penetration and absorption properties, with the lowertheir mass concentrations,the faster transdermal permeation.
引文
[1]郑依玲,董鹏鹏,梅全喜.桃胶特性化学成分药理作用及临床应用研究进展[J].时珍国医国药,2017,28(7):1728.
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[11]张宏梅,崔佰吉,钱曦垚,等.苯磺酸氨氯地平乳剂凝胶的制备及其体外透皮吸收的考察[J].中国新药杂志,2016,25(3):348.
[12]丁婷,杨建华,路新卫,等.桃胶粗多糖对糖尿病小鼠免疫调节和血糖影响初探[J].食品工业,2009,30(6):7.
[13]丁婷,王飞,韦莉萍,等.桃胶多糖对2型糖尿病大鼠血糖和血脂的影响[J].中医药导报,2010,16(5):8.
[14]王飞,丁婷,谷豪,等.桃胶多糖降糖机制研究[J].中医药导报,2012,18(8):85.
[15]贾艳丽,杜世新,陈文,等.中药桃胶对Ⅱ度烧伤暴露创面的影响[J].基层医学论坛,2008,12(34):1057.
[2]黄雪松.桃胶的性质、加工及其开发利用[J].特产研究,2004(1):47.
[3]王兆梅,李琳,郭祀远,等.生物活性多糖在化妆品中的应用[J].日用化学工业,2004(4):245.
[4]洪晓云.几种植物天然产物在化妆品上的应用[J].亚热带植物科学,2017,46(3):297.
[5]倪志华,李云凤,徐陞梅.茯苓多糖吸湿保湿性能的研究[J].山东化工,2015,44(21):17.
[6]黄仁术,谷仿丽,何晓梅.金荞麦多糖的吸湿保湿性能研究[J].化工新型材料,2014,42(6):141.
[7]高瑞英,张秀宇,慕丹,等.透明质酸等化妆品用生物活性多糖吸湿保湿性能测定[J].广东化工,2009,36(10):230.
[8]蔡延渠,邓剑壕,朱志东,等.不同产地原桃胶的多糖含量研究[J].广东药科大学学报,2018,34(1):1.
[9]廖永丽,陈景斯,郭华敏,等.不同产地油茶果壳多糖含量的研究[J].广东药科大学学报,2017,33(5):620.
[10]江敏瑜,闫丹,陈娇,等.三七跌打软膏的制备及体外透皮特性研究[J].中草药,2017,48(22):4639.
[11]张宏梅,崔佰吉,钱曦垚,等.苯磺酸氨氯地平乳剂凝胶的制备及其体外透皮吸收的考察[J].中国新药杂志,2016,25(3):348.
[12]丁婷,杨建华,路新卫,等.桃胶粗多糖对糖尿病小鼠免疫调节和血糖影响初探[J].食品工业,2009,30(6):7.
[13]丁婷,王飞,韦莉萍,等.桃胶多糖对2型糖尿病大鼠血糖和血脂的影响[J].中医药导报,2010,16(5):8.
[14]王飞,丁婷,谷豪,等.桃胶多糖降糖机制研究[J].中医药导报,2012,18(8):85.
[15]贾艳丽,杜世新,陈文,等.中药桃胶对Ⅱ度烧伤暴露创面的影响[J].基层医学论坛,2008,12(34):1057.