桃胶改良前后的多糖含量、功能性质以及体外释放度变化研究
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摘要
目的分析桃胶改良前后的多糖含量、影响制剂的功能性质以及体外释放度变化情况,探索其变化规律,为改良桃胶作为新型缓释辅料的应用提供依据。方法将不同产地原桃胶与改良桃胶烘干、粉碎、过筛,粉末用超纯水超声溶解,采用苯酚-硫酸法结合紫外-可见分光光度法测定多糖质量分数;p H计测定胶液的酸碱度;Brookfield DV-II Pro黏度计测定胶液的动力黏度;称重法测定胶粉溶解度及溶胀度;干燥器控制法测定胶粉不同相对湿度环境下的吸湿率;体外释放度实验结合HPLC法测定喘平方胶片中3个指标成分(麻黄碱、伪麻黄碱和东莨菪碱)的体外累积释放度。结果贵州习水、江西信丰、湖北随县产原桃胶各指标为多糖质量分数为84.30%、81.52%、77.84%;1%胶溶液p H值为5.63、5.60、5.88;1%胶溶液动力黏度为30.8、28.5、25.9 m Pa·s;水中溶解度为60.8、57.5、51.2 mg/g;溶胀度为34.22%、29.66%、26.25%;12 h的相对湿度(RH)43%吸湿率为28.19%、27.02%、26.44%,RH 81%吸湿率为38.22%、35.26%、34.64%;喘平方胶片中麻黄碱、伪麻黄碱和东莨菪碱2 h的体外累积释放度为95.41%～98.84%、96.05%～97.11%、96.35%～98.21%。贵州习水、江西信丰、湖北随县产改良桃胶,各指标为多糖质量分数为96.92%、94.76%、93.65%;1%胶溶液p H值为7.07、7.08、7.12;1%胶溶液动力黏度为318.4、289.6、266.4 m Pa·s;水中溶解度为178.2、167.3、163.8 mg/g;溶胀度为84.98%、81.55%、79.82%;12 h的RH 43%吸湿率为41.22%、39.93%、39.20%,RH 81%吸湿率为60.88%、58.48%、57.17%;喘平方胶片中麻黄碱、伪麻黄碱和东莨菪碱12 h的体外累积释放度为97.88%～98.36%、97.59%～98.56%、97.72%～98.12%。结论与原桃胶相比,改良桃胶在多糖含量、溶解度、溶胀度、动力黏度、吸湿率、缓释性能等方面均显著提高,且溶液呈现中性,提示可作为新型缓释材料进一步研发、应用。
Objective To analyze the difference of polysaccharides content,property characterization,and in vitro release between the original peach gum and improved peach gum,and to find the change rule,so as to provide the basis for its further application as the new delivery material.Methods The content of polysaccharides was measured by the phenol-sulfuric acid method combined with ultraviolet spectrophotometry.The p H value of liquid cement was measured by p H meter.The dynamic viscosity of liquid cement was measured by the Brookfield DV-Pro viscometer.The solubility and swelling Ⅱ ratio of rubber powder were measured by the weighing method.The moisture-absorption rate at different relative humidity of rubber powder was measured by the method of controlling humidity with the desiccator.With three index components(ephedrine,pseudoephedrine,and scopolamine) as the standards,HPLC and the release test were applied to determine the accumulated release rate of Chuanping adhesive tablets(CAT).Results Original peach gum: In the three producing areas of Xishui in Guizhou Province,Xinfeng in Jiangxi Province,and Suixian in Hubei Province,the content of polysaccharides respectively was 84.30%,81.52%,and 77.84%.The p H value of 1% liquid cement was 5.63,5.60,and 5.88.The dynamic viscosity of 1% liquid cement was 30.8,28.5,and 25.9 m Pa·s.The solubility of rubber powder was 6.08%,5.75%,and 5.12%.The swelling ratio of rubber powder was 34.22%,29.66%,and 26.25%.The 12 h moisture-absorption rate of the improved peach gum polysaccharose at RH of 43% was 28.19%,27.02%,and 26.44%; and at RH of 81% was 38.22%,35.26%,and 34.64%.The 2 h cumulative release amount in vitro of methamphetamine,pseudoephedrine,and scopolamine of Chuanping adhesive tablets were 95.41%—98.84%,96.05%—97.11%,and 96.35%—98.21%.Improved peach gum: In the three producing areas of Xishui,Xinfeng,and Suixian,the content of polysaccharides respectively was 96.92%,94.76%,and 93.35%.The p H of 1% liquid cement was 7.10,7.08,and 7.12.The dynamic viscosity of 1% liquid cement was 318.4,289.6,and 266.4 m Pa·s.The solubility of rubber powder was 17.82,16.73,and 16.38 g.The swelling ratio of rubber powder was 84.98%,81.55%,and 79.82%.The 12 h moisture-absorption rate of the improved peach gum polysaccharose at RH of 43% was 41.22%,39.93%,and 39.20%; and at RH of 81% was 60.88%,58.48%,and 57.17%.The 12 h cumulative release amount in vitro of methamphetamine,pseudoephedrine,and scopolamine of CAT were 97.88%—98.36%,97.59%—98.56%,and 97.72%—98.12%.Conclusion Compared with the original peach each gum,the content of polysaccharides,dynamic viscosity,solubility,swelling ratio,and moisture-absorption rate,and sustained release property of the improved peach gum were all improved remarkably,and the solution was also neutral.These results show that the improved peach gum can be further developed and applied as a new sustained release material.
Objective To analyze the difference of polysaccharides content,property characterization,and in vitro release between the original peach gum and improved peach gum,and to find the change rule,so as to provide the basis for its further application as the new delivery material.Methods The content of polysaccharides was measured by the phenol-sulfuric acid method combined with ultraviolet spectrophotometry.The p H value of liquid cement was measured by p H meter.The dynamic viscosity of liquid cement was measured by the Brookfield DV-Pro viscometer.The solubility and swelling Ⅱ ratio of rubber powder were measured by the weighing method.The moisture-absorption rate at different relative humidity of rubber powder was measured by the method of controlling humidity with the desiccator.With three index components(ephedrine,pseudoephedrine,and scopolamine) as the standards,HPLC and the release test were applied to determine the accumulated release rate of Chuanping adhesive tablets(CAT).Results Original peach gum: In the three producing areas of Xishui in Guizhou Province,Xinfeng in Jiangxi Province,and Suixian in Hubei Province,the content of polysaccharides respectively was 84.30%,81.52%,and 77.84%.The p H value of 1% liquid cement was 5.63,5.60,and 5.88.The dynamic viscosity of 1% liquid cement was 30.8,28.5,and 25.9 m Pa·s.The solubility of rubber powder was 6.08%,5.75%,and 5.12%.The swelling ratio of rubber powder was 34.22%,29.66%,and 26.25%.The 12 h moisture-absorption rate of the improved peach gum polysaccharose at RH of 43% was 28.19%,27.02%,and 26.44%; and at RH of 81% was 38.22%,35.26%,and 34.64%.The 2 h cumulative release amount in vitro of methamphetamine,pseudoephedrine,and scopolamine of Chuanping adhesive tablets were 95.41%—98.84%,96.05%—97.11%,and 96.35%—98.21%.Improved peach gum: In the three producing areas of Xishui,Xinfeng,and Suixian,the content of polysaccharides respectively was 96.92%,94.76%,and 93.35%.The p H of 1% liquid cement was 7.10,7.08,and 7.12.The dynamic viscosity of 1% liquid cement was 318.4,289.6,and 266.4 m Pa·s.The solubility of rubber powder was 17.82,16.73,and 16.38 g.The swelling ratio of rubber powder was 84.98%,81.55%,and 79.82%.The 12 h moisture-absorption rate of the improved peach gum polysaccharose at RH of 43% was 41.22%,39.93%,and 39.20%; and at RH of 81% was 60.88%,58.48%,and 57.17%.The 12 h cumulative release amount in vitro of methamphetamine,pseudoephedrine,and scopolamine of CAT were 97.88%—98.36%,97.59%—98.56%,and 97.72%—98.12%.Conclusion Compared with the original peach each gum,the content of polysaccharides,dynamic viscosity,solubility,swelling ratio,and moisture-absorption rate,and sustained release property of the improved peach gum were all improved remarkably,and the solution was also neutral.These results show that the improved peach gum can be further developed and applied as a new sustained release material.
引文
[1]郑依玲,董鹏鹏,梅全喜.桃胶特性化学成分药理作用及临床应用研究进展[J].时珍国医国药,2017,28(7):1728-1730.
[2]黄雪松.桃胶的性质、加工及其开发利用[J].特产研究,2004(1):47-51.
[3]蔡延渠,朱盛山.一种改良桃胶缓释材料的制备方法及其应用:中国,201510773522.X[P].2018-05-01.
[4]田先地,朱盛山,吴思平,等.改良桃胶的不同配伍对复杂成分均衡释放的影响[J].中草药,2015,46(8):1140-1144.
[5]廖永丽,陈景斯,郭华敏,等.不同产地油茶果壳多糖含量的研究[J].广东药科大学学报,2017,33(5):1-3.
[6]蔡延渠,邓剑壕,朱志东,等.不同产地原桃胶的多糖含量研究[J].广东药科大学学报,2018,34(1):25-28.
[7]中国药典[S].二部.2015.
[8]郜爱玲,王森,王立新,等.扁桃胶溶解特性的研究[J].食品工业科技,2012,33(5):56-58.
[9]杜聪,贾晓辉,沈青.HPMC水凝胶溶胀性能的影响因素研究[J].纤维素科学与技术,2011,19(1):47-51.
[10]吴思平,蔡延渠,田先地,等.桃胶改良前后的溶胀性能分析[J].中国实验方剂学杂志,2015,21(20):13-16.
[11]倪志华,李云凤,徐陞梅.茯苓多糖吸湿保湿性能的研究[J].山东化工,2015,44(21):17-18.
[12]黄仁术,谷仿丽,何晓梅.金荞麦多糖的吸湿保湿性能研究[J].化工新型材料,2014,42(6):141-142.
[13]蔡延渠,陈健,谢吉福,等.基于指纹图谱喘平缓释片复杂成分均衡释放的评价[J].中国中药杂志,2013,38(9):1360-1365.
[14]蔡延渠,刘静,李苑新,等.不同辅料配伍喘平方提取物缓释片的均衡释放研究[J].中草药,2014,45(6):774-777.
[15]蔡延渠,张志鹏,吴燕红,等.喘平缓释片体外释放与人体内吸收的相关性研究[J].中国中药杂志,2013,38(20):3473-3478.
[2]黄雪松.桃胶的性质、加工及其开发利用[J].特产研究,2004(1):47-51.
[3]蔡延渠,朱盛山.一种改良桃胶缓释材料的制备方法及其应用:中国,201510773522.X[P].2018-05-01.
[4]田先地,朱盛山,吴思平,等.改良桃胶的不同配伍对复杂成分均衡释放的影响[J].中草药,2015,46(8):1140-1144.
[5]廖永丽,陈景斯,郭华敏,等.不同产地油茶果壳多糖含量的研究[J].广东药科大学学报,2017,33(5):1-3.
[6]蔡延渠,邓剑壕,朱志东,等.不同产地原桃胶的多糖含量研究[J].广东药科大学学报,2018,34(1):25-28.
[7]中国药典[S].二部.2015.
[8]郜爱玲,王森,王立新,等.扁桃胶溶解特性的研究[J].食品工业科技,2012,33(5):56-58.
[9]杜聪,贾晓辉,沈青.HPMC水凝胶溶胀性能的影响因素研究[J].纤维素科学与技术,2011,19(1):47-51.
[10]吴思平,蔡延渠,田先地,等.桃胶改良前后的溶胀性能分析[J].中国实验方剂学杂志,2015,21(20):13-16.
[11]倪志华,李云凤,徐陞梅.茯苓多糖吸湿保湿性能的研究[J].山东化工,2015,44(21):17-18.
[12]黄仁术,谷仿丽,何晓梅.金荞麦多糖的吸湿保湿性能研究[J].化工新型材料,2014,42(6):141-142.
[13]蔡延渠,陈健,谢吉福,等.基于指纹图谱喘平缓释片复杂成分均衡释放的评价[J].中国中药杂志,2013,38(9):1360-1365.
[14]蔡延渠,刘静,李苑新,等.不同辅料配伍喘平方提取物缓释片的均衡释放研究[J].中草药,2014,45(6):774-777.
[15]蔡延渠,张志鹏,吴燕红,等.喘平缓释片体外释放与人体内吸收的相关性研究[J].中国中药杂志,2013,38(20):3473-3478.