不同提取方法对油茶果壳多糖含量的影响
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摘要
【目的】比较不同提取方法提取的油茶果壳多糖含量的差异。【方法】采用水提醇沉法、醇水提取法、超声—乙醇提取法提取油茶果壳多糖,以苯酚—硫酸法结合紫外—可见分光光度法测定其含量。【结果】超声—乙醇提取法的提取率最高,油茶果壳多糖质量分数为5.425%;水提醇沉法的提取率最低,油茶果壳多糖质量分数为2.137%。【结论】不同提取方法提取的油茶果壳多糖含量差异较大。超声—乙醇提取法提取效果更好。
Objective To compare the difference of the contents of polysaccharide extracted from the fruit shell of Camellia oleifera by different extraction methods. Methods The water-extraction alcohol-precipitation method,alcohol-water extraction method and ultrasonic-ethanol extraction method were applied for the extraction ofpolysaccharide. Polysaccharide content was determined by phenol-sulphate acid combined with ultraviolet-visiblespectrophotometry method. Results The extraction rate of polysaccharide from fruit shell of Camellia oleifera byultrasonic-ethanol extraction method was the highest,and the mass fraction of the polysaccharide was 5.425%.The extraction rate of polysaccharide from fruit shell of Camellia oleifera by water-extraction alcohol-precipitationmethod was the lowest, and the mass fraction of the polysaccharide was 2.137%. Conclusion The contents ofpolysaccharide from fruit shell of Camellia oleifera extracted by different methods varies greatly, and theultrasonic-ethanol extraction method can achieve the highest content of the polysaccharide.
Objective To compare the difference of the contents of polysaccharide extracted from the fruit shell of Camellia oleifera by different extraction methods. Methods The water-extraction alcohol-precipitation method,alcohol-water extraction method and ultrasonic-ethanol extraction method were applied for the extraction ofpolysaccharide. Polysaccharide content was determined by phenol-sulphate acid combined with ultraviolet-visiblespectrophotometry method. Results The extraction rate of polysaccharide from fruit shell of Camellia oleifera byultrasonic-ethanol extraction method was the highest,and the mass fraction of the polysaccharide was 5.425%.The extraction rate of polysaccharide from fruit shell of Camellia oleifera by water-extraction alcohol-precipitationmethod was the lowest, and the mass fraction of the polysaccharide was 2.137%. Conclusion The contents ofpolysaccharide from fruit shell of Camellia oleifera extracted by different methods varies greatly, and theultrasonic-ethanol extraction method can achieve the highest content of the polysaccharide.
引文
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[11]周勇,易延逵,杨晓敏,等.香菇中多糖含量测定方法的比较研究[J].食品研究与开发,2016,37(13):124.
[12]胡海清,林燕,王晓玲.碱提糯米藤多糖的工艺优化[J].西南民族大学学报(自然科学版),2012,38(3):405.
[2]徐曼,陈笳鸿,汪咏梅,等.油茶果壳单宁成分的提取及其分析试验初报[J].林产化学与工业,2009,29(S1):187.
[3]柳荣祥.几种山茶属植物中化学及活性研究综述[J].茶叶,1997,23(1):22.
[4] Cheng Y T, Wu S L, Ho C Y, et al. Beneficial effects of camellia oil(Camellia oleifera Abel.)on ketoprofen-induced gastrointestinal mucosal damage through upregulation of HO-1 and VEGF[J]. J Agric Food Chem,2014,62(3):642.
[5]淦永鉴,李旭,杨莉琳,等.油茶籽壳提取物抗氧化及抗癌活性研究[J].食品工业科技,2015,36(8):171.
[6]李红冰,陈跃龙,石海峰,等.油茶种子抗肿瘤有效部位群化学成分含量的分析方法[J].时珍国医国药,2008,19(7):1610.
[7] Hu J L,Nie S P,Huang D F,et al. Extraction of saponin from Camellia oleifera cake and evaluation of its antioxidant activity[J].Int J Food Sci&Tech,2012,47(8):1676.
[8]张艳华,包淑云,年四辉,等.醋炙元胡中延胡索乙素不同提取方法比较[J].赤峰学院学报(自然科学版),2018,34(6):75.
[9]李先佳.桑叶黄酮和多糖提取、纯化和分离工艺优化[D].贵阳:贵州大学,2006.
[10]廖永丽,陈景斯,郭华敏,等.不同产地油茶果壳多糖含量的研究[J].广东药科大学学报,2017,33(5):1.
[11]周勇,易延逵,杨晓敏,等.香菇中多糖含量测定方法的比较研究[J].食品研究与开发,2016,37(13):124.
[12]胡海清,林燕,王晓玲.碱提糯米藤多糖的工艺优化[J].西南民族大学学报(自然科学版),2012,38(3):405.