刺五加皂苷对模拟失重4周大鼠血脂、血糖及免疫、肝、肾功能的影响
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摘要
目的:研究刺五加皂苷对模拟失重4周大鼠血清生化指标(血脂、血糖、肝功能、肾功能、免疫功能)的影响。方法:将50只SD大鼠随机分为空白组,模型组,高、中、低剂量组,每组10只,模型组及给药组尾吊4周后,心脏取血,检测血脂、血糖及肝、肾功能、免疫功能等生化指标。结果:模拟失重4周后,模型组大鼠胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)升高(P<0.05);甘油三酯(TG)、葡萄糖(GLU)含量明显下降(P<0.05);总蛋白(TP)、白蛋白(ALB)及白球比(A/G)降低(P<0.05,P<0.01),碱性磷酸酶(ALP)有降低趋势。刺五加皂苷高剂量对升高的TC有降低趋势,同时显著性降低模型失重组有升高趋势的CREA(P<0.05);刺五加中、低剂量不同程度升高TP、ALB及A/G、ALP。结论:刺五加皂苷对模拟失重4周所致大鼠血脂、血糖、免疫功能变化均有不同程度的改善作用,对肝、肾功能影响不大。
Objective: To study the effects of Acanthopanax Senticosus saponin on serum biochemical indexes(blood lipid, blood glucose, immune function, liver and renal function) in simulated weightlessness rats. Methods: Fifty SD rats were randomly divided into blank group, model group, high-dose groups, medium-dose group and low-dose group(n=10). The rats of the groups in addition to the blank group were imposed tail hanging. After four weeks, the rats were sacrificed to take blood. The biochemical indicators such as blood lipids, blood glucose, liver function, renal function and immune function were measured. Results: After 4 weeks of simulated weightlessness, the levels of cholesterol(TC) and low density lipoprotein cholesterol(LDL-C) in model group were significantly increased(P<0.05); the content of triglycerides and glucose(GLU) were significantly decreased(P<0.05); total protein(TP), albumin(ALB) and albumin-globulin ratio(A/G) were decreased(P<0.05, P<0.01); the content of alkaline phosphatase(ALP) was decreased. Acanthopanax senticosides saponin of high dose group had trend to decrease TC, and it could significantly decrease elevated CREA of model group(P<0.05). Acanthopanax senticosides saponin of medium and low dose group had trend to increase TP, ALB, A/G and ALP in different degrees. Conclusion: Simulated weightlessness of 4 weeks has an obvious impact on immune function, a certain impact on blood lipid and blood glucose, little on the liver and renal function, and Acanthopanax senticosidessaponin can adjust these changes.
Objective: To study the effects of Acanthopanax Senticosus saponin on serum biochemical indexes(blood lipid, blood glucose, immune function, liver and renal function) in simulated weightlessness rats. Methods: Fifty SD rats were randomly divided into blank group, model group, high-dose groups, medium-dose group and low-dose group(n=10). The rats of the groups in addition to the blank group were imposed tail hanging. After four weeks, the rats were sacrificed to take blood. The biochemical indicators such as blood lipids, blood glucose, liver function, renal function and immune function were measured. Results: After 4 weeks of simulated weightlessness, the levels of cholesterol(TC) and low density lipoprotein cholesterol(LDL-C) in model group were significantly increased(P<0.05); the content of triglycerides and glucose(GLU) were significantly decreased(P<0.05); total protein(TP), albumin(ALB) and albumin-globulin ratio(A/G) were decreased(P<0.05, P<0.01); the content of alkaline phosphatase(ALP) was decreased. Acanthopanax senticosides saponin of high dose group had trend to decrease TC, and it could significantly decrease elevated CREA of model group(P<0.05). Acanthopanax senticosides saponin of medium and low dose group had trend to increase TP, ALB, A/G and ALP in different degrees. Conclusion: Simulated weightlessness of 4 weeks has an obvious impact on immune function, a certain impact on blood lipid and blood glucose, little on the liver and renal function, and Acanthopanax senticosidessaponin can adjust these changes.
引文
[1]叶小泉.名扬四海的刺五加.开卷有益:求医问药,1998(10):37
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[3]李勇枝,石宏志,范全春,等.强骨抗萎方对模拟失重大鼠骨及相关组织生化指标的影响,航天医学与医学工程,2003,16(2):103-106
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[6]陈剑峰,张烽.剌五加皂苷对体外培养人鼠脊髓运动神经元缺氧损伤的保护作用.第二军医大学学报,2006,27(2):173-177
[7]徐叔云,卞如濂,陈修.药理实验方法学.北京:人民卫生出版社,2002:1900
[8]陈曜,陈恩强,唐红.体检人群血脂和血糖检测结果与体重指数的相关性分析.华西医学,2010,25(3):487-490
[9]Iso H,Qizibash N,Demeurisse G,et al.Serum cholesterol levels and six-year mortality from stroke in 350 977 men screened for the multiple risk factor interventiontrial.N Engl J Med,1989,18(320):904-910
[10]阮晓霞,胡蓉.血脂异常与脏器出血关系的研究进展.心血管病学进展,2015,36(2):176-179
[11]Valeria Gasperi,Cinzia Rapino,Natalia Battista,et al.A functional interplay between 5-Lipoxygenase and u-Calpain affects survival and cytokine profile of human jurkat T lymphocyte exposed to simulated microgravity.Bio Med Research International,2014:1-10
[12]Lijun Wei,Chuanpeng Liu,Li Kang,et al.Experimental study on effect of simulated microgravity on structural chromosome instability of human peripheral blood lymphocytes.PLOS ONE,2014,9(6):1-12
[13]Emily M Martinez,Miya C Yoshida,Tara Lynne T Candelario,et al.Spaceflight and simulated microgravity cause a significant reduction of key gene expression in early T-cell activation.Am J Physiol Regul Integr Comp Physiol,2015,308(6):480-488
[14]杨光华,温秀兰,王宝珍,等.模拟失重(HDT—30°)和噪声复合因素对大鼠神经-内分泌-免疫功能的影响.空间科学学报,1994,14(3):210-213.
[15]Nakamura H I,Kumei Y,Morita S,et al.Antagonism between apoptotic(Bax/Bcl-2)and anti-apoptotic(IAP)signals in human osteoblastic cells under vector-averaged gravity condition.Ann N Y Acad Sci,2003,1010:143-147
[16]Van Den Bos T,Beertsen W.Baund phosphoproteins cuhancem inera liza tion of a lka line phospha ta se co llagen comp lexes in vivo.J Bone Miner Res,1994,9(8):1205-1209
[17]张林,谢鑫,王俊岩,等.补肾和补肾健脾方对尾部悬吊大鼠股骨骨丢失的作用研究.中华中医药杂志,2014,29(3):700-703
[18]杨锐,黄震,蔡德鸿.雷奈酸锶对模拟失重雄性大鼠骨质疏松的防治作用.南方医科大学学报,2010,30(4):834-837
[19]宋艳,赵国桢,汪德生.不同时机介入电针对模拟失重大鼠肝脏组织结构、功能和一氧化氮水平的影响.北京中医药大学学报,2015,38(7):481-486
[20]刘玉盛,黄伟芬,李丽萍.21d-6°头低位卧床期间运动训练对骨丢失的防护效应.航天医学与医学工程,2003,16(2):96-99
[2]陈杰,马进,丁兆平,等.一种模拟长期失重影响的大鼠尾部悬吊模型.空间科学学报,1993,13(2):159-162
[3]李勇枝,石宏志,范全春,等.强骨抗萎方对模拟失重大鼠骨及相关组织生化指标的影响,航天医学与医学工程,2003,16(2):103-106
[4]Huang L,Zhao H,Huang B,et al.Acanthopanax senticosus:review of botany,chemistry and pharmacology.Pharmazie,2011,66(2):83-97
[5]谢新,狄留庆.刺五加叶化学成分和药理作用研究进展.中国现代中药,2008,10(11):6-8
[6]陈剑峰,张烽.剌五加皂苷对体外培养人鼠脊髓运动神经元缺氧损伤的保护作用.第二军医大学学报,2006,27(2):173-177
[7]徐叔云,卞如濂,陈修.药理实验方法学.北京:人民卫生出版社,2002:1900
[8]陈曜,陈恩强,唐红.体检人群血脂和血糖检测结果与体重指数的相关性分析.华西医学,2010,25(3):487-490
[9]Iso H,Qizibash N,Demeurisse G,et al.Serum cholesterol levels and six-year mortality from stroke in 350 977 men screened for the multiple risk factor interventiontrial.N Engl J Med,1989,18(320):904-910
[10]阮晓霞,胡蓉.血脂异常与脏器出血关系的研究进展.心血管病学进展,2015,36(2):176-179
[11]Valeria Gasperi,Cinzia Rapino,Natalia Battista,et al.A functional interplay between 5-Lipoxygenase and u-Calpain affects survival and cytokine profile of human jurkat T lymphocyte exposed to simulated microgravity.Bio Med Research International,2014:1-10
[12]Lijun Wei,Chuanpeng Liu,Li Kang,et al.Experimental study on effect of simulated microgravity on structural chromosome instability of human peripheral blood lymphocytes.PLOS ONE,2014,9(6):1-12
[13]Emily M Martinez,Miya C Yoshida,Tara Lynne T Candelario,et al.Spaceflight and simulated microgravity cause a significant reduction of key gene expression in early T-cell activation.Am J Physiol Regul Integr Comp Physiol,2015,308(6):480-488
[14]杨光华,温秀兰,王宝珍,等.模拟失重(HDT—30°)和噪声复合因素对大鼠神经-内分泌-免疫功能的影响.空间科学学报,1994,14(3):210-213.
[15]Nakamura H I,Kumei Y,Morita S,et al.Antagonism between apoptotic(Bax/Bcl-2)and anti-apoptotic(IAP)signals in human osteoblastic cells under vector-averaged gravity condition.Ann N Y Acad Sci,2003,1010:143-147
[16]Van Den Bos T,Beertsen W.Baund phosphoproteins cuhancem inera liza tion of a lka line phospha ta se co llagen comp lexes in vivo.J Bone Miner Res,1994,9(8):1205-1209
[17]张林,谢鑫,王俊岩,等.补肾和补肾健脾方对尾部悬吊大鼠股骨骨丢失的作用研究.中华中医药杂志,2014,29(3):700-703
[18]杨锐,黄震,蔡德鸿.雷奈酸锶对模拟失重雄性大鼠骨质疏松的防治作用.南方医科大学学报,2010,30(4):834-837
[19]宋艳,赵国桢,汪德生.不同时机介入电针对模拟失重大鼠肝脏组织结构、功能和一氧化氮水平的影响.北京中医药大学学报,2015,38(7):481-486
[20]刘玉盛,黄伟芬,李丽萍.21d-6°头低位卧床期间运动训练对骨丢失的防护效应.航天医学与医学工程,2003,16(2):96-99