艾灸治疗神经根炎模型大鼠抗炎镇痛效应研究
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摘要
目的:研究艾灸对腰椎间盘突出(LDH)的抗炎镇痛作用。方法:复制化学性神经根炎模型大鼠模拟LDH,艾灸干预14 d进行Siegal神经功能评分,采血测定IL-1β水平,HE染色观察神经根及周围肌肉组织炎症反应。结果:艾灸可显著降低模型大鼠神经功能评分,改善下肢功能障碍症状,降低血清IL-1β水平,减轻组织炎症细胞浸润,缓解化学性炎症对神经根的损害。结论:艾灸具有良好的抗炎镇痛作用,该作用可能是其临床治疗LDH的机制之一。
Objective: To observe the anti-inflammatory and analgesic effects of moxibustion in experimental with Lumbar disc herniation. Methods: We surgically duplicated a chemical radicular neuritis model to simulate lumbar disc herniation. The scores of Siegal neurological function were measured on postoperative days(PODs) 2 and 14. Inflammatory factor IL-1β level in the serum and the histology of the nerve root were determined on PODs 14. Results: Moxibustion can significantly reduce the scores of Siegal neurological function to improve the symptoms of lower limb dysfunction in model rats, decrease the level of serum inflammatory cytokines IL-1β, inhibit nerve root tissue and surrounding tissue inflammation cell infiltration, to reduce the chemical inflammation of the nerve root damage. Conclusion: Moxibustion has anti-inflammatory and analgesic effects that can alleviate the discomfort of lumbar disc herniation.
Objective: To observe the anti-inflammatory and analgesic effects of moxibustion in experimental with Lumbar disc herniation. Methods: We surgically duplicated a chemical radicular neuritis model to simulate lumbar disc herniation. The scores of Siegal neurological function were measured on postoperative days(PODs) 2 and 14. Inflammatory factor IL-1β level in the serum and the histology of the nerve root were determined on PODs 14. Results: Moxibustion can significantly reduce the scores of Siegal neurological function to improve the symptoms of lower limb dysfunction in model rats, decrease the level of serum inflammatory cytokines IL-1β, inhibit nerve root tissue and surrounding tissue inflammation cell infiltration, to reduce the chemical inflammation of the nerve root damage. Conclusion: Moxibustion has anti-inflammatory and analgesic effects that can alleviate the discomfort of lumbar disc herniation.
引文
[1] NOTOHAMIPRODJO S, STAHL R, BRAUNAGEL M, et al. Diagnostic accuracy of contemporary multidetector computed tomography (MDCT) for the detection of lumbar disc herniation[J]. European radiology, 2017, 27(8): 3443-3451.
[2] DI MARTINO A, MERLINI L, FALDINI C. Autoimmunity in intervertebral disc herniation: from bench to bedside[J]. Expert opinion on therapeutic targets, 2013, 17(12): 1461-1470.
[3] SADOWSKA A, TOULI E, HITZL W, et al. Inflammaging in cervical and lumbar degenerated intervertebral discs: analysis of proinflammatory cytokine and TRP channel expression[J]. Eur Spine J, 2017.
[4] XU D, SUN Y, BAO G, et al. MMP-1 overexpression induced by IL-1beta: possible mechanism for inflammation in degenerative lumbar facet joint[J]. Journal of orthopaedic science: official journal of the Japanese Orthopaedic Association, 2013, 18(6): 1012-1019.
[5] PAZ APARICIO J, FERNANDEZ BANCES I, LOPEZ-ANGLADA FERNANDEZ E, et al. The IL-1beta (+3953 T/C) gene polymorphism associates to symptomatic lumbar disc herniation[J]. Eur Spine J, 2011, 20(Suppl 3):383-389.
[6] RAMASWAMI R, GHOGAWALA Z, WEINSTEIN J N. Management of Sciatica[J]. The New England journal of medicine, 2017, 376(12): 1175-1177.
[7] XING Q, HUANG Z, ZHANG J. Anti-inflammatory and analgesic effects of yaotuitong capsules in experimental rats with chemically induced radicular neuritis[J]. Journal of traditional Chinese medicine=Chung it sachih ying wen pan, 2012, 32(3): 437-441.
[8] 刘荣芬, 姜亚梅. 华佗夹脊穴的针刺镇痛机理探讨[J]. 中国中医基础医学杂志, 2008, 14(12): 943.
[9] 谢艳艳, 吴耀持. 温针对腰神经根受压模型大鼠组织形态的影响[J]. 中国组织工程研究, 2015, 19(49): 7873-7878.
[10] SIEGAL T, SIEGAL T, SHAPIRA Y, et al. Indomethacin and dexamethasone treatment in experimental neoplastic spinal cord compression: Part 1. Effect on water content and specific gravity[J]. Neurosurgery, 1988, 22(2): 328-333.
[11] 王美琴. 针刀干预对腰椎间盘突出症模型大鼠背根神经节微循环影响的实验研究 [D].北京:北京中医药大学, 2016.
[12] 李力夫, 王维, 陈建冲. 十枣汤治疗腰椎间盘突出症顽固性放射痛15例[J]. 中国中医基础医学杂志, 2011, 17(11): 1286.
[13] 谭伟伟, 何升华. 腰痛—历史沿革与文献探微[J]. 实用中医内科杂志, 2016, 30(03): 87-88.
[14] 黄萍萍, 廖军, 郑佳璇, 等. 艾灸疗法治疗腰腿痛的文献研究[J]. 时珍国医国药, 2015, 26(02): 407-408.
[2] DI MARTINO A, MERLINI L, FALDINI C. Autoimmunity in intervertebral disc herniation: from bench to bedside[J]. Expert opinion on therapeutic targets, 2013, 17(12): 1461-1470.
[3] SADOWSKA A, TOULI E, HITZL W, et al. Inflammaging in cervical and lumbar degenerated intervertebral discs: analysis of proinflammatory cytokine and TRP channel expression[J]. Eur Spine J, 2017.
[4] XU D, SUN Y, BAO G, et al. MMP-1 overexpression induced by IL-1beta: possible mechanism for inflammation in degenerative lumbar facet joint[J]. Journal of orthopaedic science: official journal of the Japanese Orthopaedic Association, 2013, 18(6): 1012-1019.
[5] PAZ APARICIO J, FERNANDEZ BANCES I, LOPEZ-ANGLADA FERNANDEZ E, et al. The IL-1beta (+3953 T/C) gene polymorphism associates to symptomatic lumbar disc herniation[J]. Eur Spine J, 2011, 20(Suppl 3):383-389.
[6] RAMASWAMI R, GHOGAWALA Z, WEINSTEIN J N. Management of Sciatica[J]. The New England journal of medicine, 2017, 376(12): 1175-1177.
[7] XING Q, HUANG Z, ZHANG J. Anti-inflammatory and analgesic effects of yaotuitong capsules in experimental rats with chemically induced radicular neuritis[J]. Journal of traditional Chinese medicine=Chung it sachih ying wen pan, 2012, 32(3): 437-441.
[8] 刘荣芬, 姜亚梅. 华佗夹脊穴的针刺镇痛机理探讨[J]. 中国中医基础医学杂志, 2008, 14(12): 943.
[9] 谢艳艳, 吴耀持. 温针对腰神经根受压模型大鼠组织形态的影响[J]. 中国组织工程研究, 2015, 19(49): 7873-7878.
[10] SIEGAL T, SIEGAL T, SHAPIRA Y, et al. Indomethacin and dexamethasone treatment in experimental neoplastic spinal cord compression: Part 1. Effect on water content and specific gravity[J]. Neurosurgery, 1988, 22(2): 328-333.
[11] 王美琴. 针刀干预对腰椎间盘突出症模型大鼠背根神经节微循环影响的实验研究 [D].北京:北京中医药大学, 2016.
[12] 李力夫, 王维, 陈建冲. 十枣汤治疗腰椎间盘突出症顽固性放射痛15例[J]. 中国中医基础医学杂志, 2011, 17(11): 1286.
[13] 谭伟伟, 何升华. 腰痛—历史沿革与文献探微[J]. 实用中医内科杂志, 2016, 30(03): 87-88.
[14] 黄萍萍, 廖军, 郑佳璇, 等. 艾灸疗法治疗腰腿痛的文献研究[J]. 时珍国医国药, 2015, 26(02): 407-408.