绵羊Wnt2蛋白生物信息学分析
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摘要
Wnt2蛋白是Wnts蛋白家族的重要成员,参与卵巢的发育。本研究以NCBI蛋白质数据库中发布的Wnt2蛋白氨基酸序列为研究对象,采用生物信息学软件对Wnt2蛋白的理化性质、信号肽及跨膜结构域、糖基化和磷酸化位点、二级结构和功能结构域、亚细胞定位和功能结构分类、序列相似比对及聚类、三级结构进行预测分析。结果发现,绵羊Wnt2由360个氨基酸组成,其等电点为9.21,有糖基化位点和磷酸化位点,是一个有信号肽的稳定的蛋白;二级结构与三级结构一致,均显示Wnt2由α-螺旋、延伸链、无规则卷曲构成;绵羊Wnt2蛋白是细胞外蛋白,主要参与信号转导和转录调控。研究结果为深入探讨Wnt2基因及其编码蛋白的结构和功能提供相关依据。
Wnt2 protein is an important member of Wnts protein family and is involved in the development of ovarian. In this study, the amino acid sequence of Wnt2 protein published in NCBI protein database was used as the research object. Bioinformatics software was used to predict and analyze the physicochemical property,signal peptide and transmembrane region, glycosylation and phosphorylation site, secondary structure and functional domain, subcellular localization and functional category, sequence similarity comparison and clustering,tertiary structure of Wnt2 protein. The results showed that the Wnt2 protein in sheep was a stable protein with signal peptide, composed of 360 amino acids and with glycosylation site and phosphorylation site. The isoelectric point was 9.21. The secondary structure was composed of α helix, extended chain and irregular coil, and the tertiary structure was basically consistent with it. Wnt2 protein in sheep was extracellular protein, involved in signal transduction and transcription regulation. These results would provide theoretical basis for further study on Wnt2 gene and the structure and function of encoded protein.
Wnt2 protein is an important member of Wnts protein family and is involved in the development of ovarian. In this study, the amino acid sequence of Wnt2 protein published in NCBI protein database was used as the research object. Bioinformatics software was used to predict and analyze the physicochemical property,signal peptide and transmembrane region, glycosylation and phosphorylation site, secondary structure and functional domain, subcellular localization and functional category, sequence similarity comparison and clustering,tertiary structure of Wnt2 protein. The results showed that the Wnt2 protein in sheep was a stable protein with signal peptide, composed of 360 amino acids and with glycosylation site and phosphorylation site. The isoelectric point was 9.21. The secondary structure was composed of α helix, extended chain and irregular coil, and the tertiary structure was basically consistent with it. Wnt2 protein in sheep was extracellular protein, involved in signal transduction and transcription regulation. These results would provide theoretical basis for further study on Wnt2 gene and the structure and function of encoded protein.
引文
Alexandre B.,Alan K.G.,and Derek B.,2009,WNT signaling in ovarian follicle biology and tumorigenesis,Trends Endocrinol.Metab.,21(1):25-32
Katoh M.,2001,Frequent up-regulation of WNT2 in primary gastric cancer and colorectal cancer,INT J.Oncol.,19(5):1003-1007
Kikuchi A.,Yamamoto H.,and Kishida S.,2007,Multiplicity of the interactions of Wnt proteins and their receptors,Cell.Signal.,19(4):659-671
Komekado H.,Yamamoto H.,Chiba T.,and Kikuchi A.,2007,Gycosylation and palmitoylation of Wnt-3a are coupled to produce an active form of Wnt-3a,Genes Cells,12(4):521-534
Kristen S.,Margaret S.,James S.,Duncan D.,and Paula M.,2008,3D representation of Wnt and frizzled gene expression patterns in the mouse embryo at embryonic day 11.5(Ts19),Gene Expr.Patterns,8:331-348
Li L.,Ji S.Y.,Yang J.L.,Li X.X.,Zhang J.,Zhang Y.,and Hu Z.Y.,2014,Wnt/β-catenin signaling regulates follicular development by modulating the expression of foxo3a signaling components,Mol.Cell.Endocrinol.,382(2):915-925
Logan C.Y.,and Nusse R.,2004,The Wnt signaling pathway in development and disease,Annu.Rev.Cell Dev.Biol.,20:781-810
Petersen T.N.,Brunak S.,Heijne G.,and Nielsen H.,2011,Signal P4.0:discriminating signal peptides from transmembrane regions,Nat.Methods,8(10):785-786
Xiong W.,Zhang X.J.,Zhang H.Y.,Xu T.,Xu Y.,Yang H.J.,and Du Y.M.,2015,Analysis of the structure and function of human mitochondrial transcription termination factor 3 based on bioinformatic methods,Shengwu Jishu Tongxun(Letters in Biotechnology),26(3):367-373(熊伟,张晓娟,张海洋,徐彤,徐优,杨红娟,杜亚蔓,2015,基于生物信息学方法预测人线粒体转录终止因子3蛋白的结构与功能,生物技术通讯,26(3):367-373)
Wang H.X.,Gillio-Meina C.,Chen S.,Gong X.Q.,Li T.Y.,Bai D.,and Kidder G.M.,2013,The canonical WNT2 pathway and FSH interact to regulate gap junction assembly in mouse granulosa cells,Biol.Reprod.,89(2):39
Wang H.X.,Li T.Y.,and Kidder G.M.,2010,WNT2 regulates DNA synthesis in mouse granulosa cells through beta-catenin,Biol.Reprod.,82(5):867-875
Wang H.X.,Tekpetey F.R.,and Kidder G.M.,2009,Identification of WNT/beta-CATENIN signaling pathway components in human cumulus cells,Mol.Hum.Reprod.,15(1):11-17
Willert K.,Brown J.D.,Danenberg E.,Duncan A.W.,Weissman I.L.,Reya T.,Yates J.R.,and Nusse R.,2003,Wnt proteins are lipid-modified and can act as stem cell growth factors,Nature,423:448-452
Wodarz A.,and Nusse R.,1998,Mechanisms of Wnt signaling in development,Annu.Rev.Cell Dev.Biol.,14:59-88
Yang Y.J.,Li H.X.,and Yang G.S.,2008,Cloning and bioinformatics analysis of pig Wnt10b cDNA,Zhongguo Shengwu Huaxue Yu Fenzi Shengwu Xuebao(Chinese Journal of Biochemistry and Molecular Biology),24(7):679-683(杨映娟,李惠侠,杨公社,2008,猪Wnt10b基因cDNA的克隆及生物信息学分析,中国生物化学与分子生物学报,24(7):679-683)
Katoh M.,2001,Frequent up-regulation of WNT2 in primary gastric cancer and colorectal cancer,INT J.Oncol.,19(5):1003-1007
Kikuchi A.,Yamamoto H.,and Kishida S.,2007,Multiplicity of the interactions of Wnt proteins and their receptors,Cell.Signal.,19(4):659-671
Komekado H.,Yamamoto H.,Chiba T.,and Kikuchi A.,2007,Gycosylation and palmitoylation of Wnt-3a are coupled to produce an active form of Wnt-3a,Genes Cells,12(4):521-534
Kristen S.,Margaret S.,James S.,Duncan D.,and Paula M.,2008,3D representation of Wnt and frizzled gene expression patterns in the mouse embryo at embryonic day 11.5(Ts19),Gene Expr.Patterns,8:331-348
Li L.,Ji S.Y.,Yang J.L.,Li X.X.,Zhang J.,Zhang Y.,and Hu Z.Y.,2014,Wnt/β-catenin signaling regulates follicular development by modulating the expression of foxo3a signaling components,Mol.Cell.Endocrinol.,382(2):915-925
Logan C.Y.,and Nusse R.,2004,The Wnt signaling pathway in development and disease,Annu.Rev.Cell Dev.Biol.,20:781-810
Petersen T.N.,Brunak S.,Heijne G.,and Nielsen H.,2011,Signal P4.0:discriminating signal peptides from transmembrane regions,Nat.Methods,8(10):785-786
Xiong W.,Zhang X.J.,Zhang H.Y.,Xu T.,Xu Y.,Yang H.J.,and Du Y.M.,2015,Analysis of the structure and function of human mitochondrial transcription termination factor 3 based on bioinformatic methods,Shengwu Jishu Tongxun(Letters in Biotechnology),26(3):367-373(熊伟,张晓娟,张海洋,徐彤,徐优,杨红娟,杜亚蔓,2015,基于生物信息学方法预测人线粒体转录终止因子3蛋白的结构与功能,生物技术通讯,26(3):367-373)
Wang H.X.,Gillio-Meina C.,Chen S.,Gong X.Q.,Li T.Y.,Bai D.,and Kidder G.M.,2013,The canonical WNT2 pathway and FSH interact to regulate gap junction assembly in mouse granulosa cells,Biol.Reprod.,89(2):39
Wang H.X.,Li T.Y.,and Kidder G.M.,2010,WNT2 regulates DNA synthesis in mouse granulosa cells through beta-catenin,Biol.Reprod.,82(5):867-875
Wang H.X.,Tekpetey F.R.,and Kidder G.M.,2009,Identification of WNT/beta-CATENIN signaling pathway components in human cumulus cells,Mol.Hum.Reprod.,15(1):11-17
Willert K.,Brown J.D.,Danenberg E.,Duncan A.W.,Weissman I.L.,Reya T.,Yates J.R.,and Nusse R.,2003,Wnt proteins are lipid-modified and can act as stem cell growth factors,Nature,423:448-452
Wodarz A.,and Nusse R.,1998,Mechanisms of Wnt signaling in development,Annu.Rev.Cell Dev.Biol.,14:59-88
Yang Y.J.,Li H.X.,and Yang G.S.,2008,Cloning and bioinformatics analysis of pig Wnt10b cDNA,Zhongguo Shengwu Huaxue Yu Fenzi Shengwu Xuebao(Chinese Journal of Biochemistry and Molecular Biology),24(7):679-683(杨映娟,李惠侠,杨公社,2008,猪Wnt10b基因cDNA的克隆及生物信息学分析,中国生物化学与分子生物学报,24(7):679-683)