结肠癌组织中Ⅱ型人回肠脂肪酸结合蛋白mRNA表达及其与癌细胞增殖、迁移的相关性
摘要
目的探讨结肠癌组织中Ⅱ型人回肠脂肪酸结合蛋白(Ⅱ型FABP6) mRNA表达及其与癌细胞增殖、迁移的相关性。方法选择2015年1月至2018年3月间在本院接受结肠癌根治术治疗的原发性结肠癌患者89例作为结肠癌组,同期在本院经结肠镜切除息肉的结肠息肉患者127例作为结肠息肉组。采用荧光定量PCR法检测结肠病灶组织中Ⅱ型FABP6mRNA表达量及结肠癌增殖、迁移相关基因表达量的差异,采用Pearson检验评估结肠癌组织中Ⅱ型FABP6 mRNA表达量与癌细胞增殖、迁移活力关系。结果结肠癌组病灶中Ⅱ型FABP6的mRNA表达量低于结肠息肉组;癌细胞增殖相关基因MS4A12 mRNA表达量低于结肠息肉组,GTPBP4、EZH2、livin、Sph K1 mRNA表达量高于结肠息肉组;癌细胞迁移相关基因Numb mRNA表达量低于结肠息肉组,Lumican、PTTG1、CHD1L mRNA表达量高于结肠息肉组。结肠癌组织中Ⅱ型FABP6 mRNA表达量与癌细胞增殖、迁移活力均呈负相关。结论Ⅱ型FABP6在结肠癌组织中的mRNA表达量明显低于结肠良性病变,且其表达量与结肠癌细胞的增殖、迁移能力呈负相关。
引文
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[3]Dermadi D,Valo S,Ollila S,et al. Western Diet Deregulates Bile Acid Homeostasis,Cell Proliferation,and Tumorigenesis in Colon[J]. Cancer Res,2017,77(12):3352-3363. DOI:10. 1158/0008-5472. CAN-16-2860.
[4]Al FMS,Gou X,Forootan SS,et al. The increased expression of fatty acid-binding protein 9 in prostate cancer and its prognostic significance[J]. Oncotarget,2016,7(50):82783-82797. DOI:10. 18632/oncotarget. 12635.
[5]Ding L,Yang L,Wang Z,et al. Bile acid nuclear receptor FXR and digestive system diseases[J]. Acta Pharm Sin B,2015,5(2):135-144. DOI:10. 1016/j. apsb. 2015. 01. 004.
[6]贺理,周冰倩,宋淑佳,等. MS4A12基因在结肠癌中的表达及对结肠癌细胞增殖的影响[J].基因组学与应用生物学,2016,35(9):2217-2221.
[7]曾沁,刘勋,陈明,等. RNAi沉默GTPBP4基因对结肠癌RKO细胞增殖及凋亡的影响[J].肿瘤防治研究,2017,44(1):23-27. DOI:10. 3971/j. issn. 1000-8578. 2017. 01. 005.
[8]Liu WB,Jia WD,Ma JL,et al. Knockdown of GTPBP4 inhibits cell growth and survival in human hepatocellular carcinoma and its prognostic significance[J]. Oncotarget,2017,8(55):93984-93997. DOI:10. 18632/oncotarget. 21500.
[9]Wang D,Quiros J,Mahuron K,et al. Targeting EZH2 Reprograms Intratumoral Regulatory T Cells to Enhance Cancer Immunity[J]. Cell Rep,2018,23(11):3262-3274. DOI:10. 1016/j.celrep. 2018. 05. 050.
[10]Petraglia F,Singh AA,Carafa V,et al. Combined HAT/EZH2modulation leads to cancer-selective cell death[J]. Oncotarget,2018,9(39):25630-25646. DOI:10. 18632/oncotarget. 25428.
[11]Liu S,Li X,Li Q,et al. Silencing Livin improved the sensitivity of colon cancer cells to 5-fluorouracil by regulating crosstalk between apoptosis and autophagy[J]. Oncol Lett,2018,15(5):7707-7715. DOI:10. 3892/ol. 2018. 8282.
[12]诸葛春凤,刘诗权,谭林,等. SphK1和FAK对人结肠癌HCT116细胞上皮间质转化的影响[J].中国病理生理杂志,2016,32(3):439-444. DOI:10. 3969/j. issn. 1000-4718. 2016.03. 009.
[13]李博,邓睿,李栋,等.结肠癌组织中SphK1和FAK表达量与临床病理特征、上皮间质转化的相关性研究[J].海南医学院学报,2018,24(2):202-205,209. DOI:10. 13210/j. cnki.jhmu. 20180105. 001.
[14]Yang CT,Li JM,Chu WK,et al. Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin[J]. Cell Death Dis,2018,9(4):414. DOI:10. 1038/s41419-017-0212-3.
[15]Vázquez-Ulloa E,Ramos-Cruz AC,Prada D,et al. Loss of nuclear NOTCH1,but not its negative regulator NUMB,is an independent predictor of cervical malignancy[J]. Oncotarget,2018,9(27):18916-18928. DOI:10. 18632/oncotarget. 24828.
[16]Colaluca IN,Basile A,Freiburger L,et al. A Numb-Mdm2 fuzzy complex reveals an isoform-specific involvement of Numb in breast cancer[J]. J Cell Biol,2018,217(2):745-762. DOI:10.1083/jcb. 201709092.
[17]Tsuda M,Cho K,Ooka M,et al. ALC1/CHD1L,a chromatin-remodeling enzyme,is required for efficient base excision repair[J]. PLo S One,2017,12(11):e0188320. DOI:10. 1371/journal. pone. 0188320.