miR-485-5p在鼻咽癌组织中的表达及临床意义
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摘要
目的探讨miR-485-5p在鼻咽癌组织中的表达及临床意义。方法采用qRT-PCR检测76例鼻咽癌和28例鼻咽部黏膜组织中miR-485-5p的表达,并结合临床信息,统计分析其表达水平与鼻咽癌患者临床病理参数的相关性。结果 miR-485-5p在鼻咽癌组织中的表达较鼻咽部黏膜组织显著下降(P<0.05);同时miR-485-5p低表达与鼻咽癌患者的淋巴结转移密切相关(P=0.001),而与鼻咽癌患者的年龄、性别、T分级和TNM分期无明显相关(P> 0.05)。结论本研究表明miR-485-5p为转移相关基因,可能在鼻咽癌转移中发挥了重要作用。
Objective To investigate the expression and clinical significance of miR-485-5p in nasopharyngeal carcinoma(NPC).Methods The expression of miR-485-5p in 76 specimens of NPC and 28 of nasopharyngeal epithelial tissue was detected with qRT-PCR, and the correlations between the expression and clinicopathological parameters were analyzed statistically.Results The expression of miR-485-5p was decreased in NPC tissues when compared with nasopharyngeal epithelial tissues(P<0.05). Low expression of miR-485-5p was closely associated with the lymph node metastasis in NPC patients(P=0.001), while not statistically associated with patients' age, gender, T classification and TNM stages of NPC(all P>0.05).Conclusion As a metastasis-associated gene,miR-485-5p may play an important role in metastasis of NPC.
Objective To investigate the expression and clinical significance of miR-485-5p in nasopharyngeal carcinoma(NPC).Methods The expression of miR-485-5p in 76 specimens of NPC and 28 of nasopharyngeal epithelial tissue was detected with qRT-PCR, and the correlations between the expression and clinicopathological parameters were analyzed statistically.Results The expression of miR-485-5p was decreased in NPC tissues when compared with nasopharyngeal epithelial tissues(P<0.05). Low expression of miR-485-5p was closely associated with the lymph node metastasis in NPC patients(P=0.001), while not statistically associated with patients' age, gender, T classification and TNM stages of NPC(all P>0.05).Conclusion As a metastasis-associated gene,miR-485-5p may play an important role in metastasis of NPC.
引文
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[19] Lin XJ,He CL,Sun T,et al.hsa-miR-485-5p reverses epithelial to mesenchymal transition and promotes cisplatin-induced cell death by targeting PAK1 in oral tongue squamous cell carcinoma[J].Int J Mol Med,2017,40 (1):83-89.
[20] Zhang SY,Gao F,Peng CG,et al.miR-485-5p promotes osteoporosis via targeting Osterix[J].Eur Rev Med Pharmacol Sci,2018,22 (15):4792-4799.
[21] Hu XX,Xu XN,He BS,et al.microRNA-485-5p Functions as a Tumor Suppressor in Colorectal Cancer Cells by Targeting CD147[J].J Cancer,2018,9 (15):2603-2611.
[22] Wang R,Zuo X,Wang K,et al.MicroRNA-485-5p attenuates cell proliferation in glioma by directly targeting paired box 3[J].Am J Cancer Res,2018,8(12):2507-2517.
[23] Jing LL,Mo XM.Reduced miR-485-5p expression predicts poor prognosis in patients with gastric cancer[J].Eur Rev Med Pharmacol Sci,2016,20(8):1516-1520.
[2] Wang M,Cai WR,Meng R,et al.miR- 485-5p suppresses breast cancer progression and chemosensitivity by targeting survivin[J].Biochem Biophys Res Commun,2018,501 (1):48-54.
[3] Mou X,Liu S.MiR-485 inhibits metastasis and EMT of lung adenocarcinoma by targeting Flot2[J].Biochem Biophys Res Commun,2016,477 (4):521-526.
[4] Lou C,Xiao M,Cheng S,et al.MiR- 485-3p and miR- 485-5p suppress breast cancer cell metastasis by inhibiting PGC-1alpha expression[J].Cell Death Dis,2016,7:e2159.
[5] Wang FR,Xu SH,Wang BM,et al.MiR- 485-5p inhibits metastasis and proliferation of osteosarcoma by targeting CX3CL1[J].Eur Rev Med Pharmacol Sci,2018,22 (21):7197-7204.
[6] Chen Z,Li Q,Wang S,et al.miR4855p inhibits bladder cancer metastasis by targeting HMGA2[J].Int J Mol Med,2015,36 (4):1136-1142.
[7] Huang RS,Zheng YL,Li C,et al.MicroRNA- 485-5p suppresses growth and metastasis in non-small cell lung cancer cells by targeting IGF2BP2[J].Life Sci,2018,199:104-111.
[8] 刘超,李果,刘勇,等.miR- 324-3p及其靶基因WNT2B在鼻咽癌组织中的表达及临床意义[J].临床耳鼻咽喉头颈外科杂志,2014,28 (21):1676-1678,1684.Liu C,Li G,Liu Y,et al.The expression and significance of miRNA- 324-3p and WNT2B in nasopharyngeal carcinoma[J].Journal of Clinical Otorhinolaryngology Head and Neck Surgery,2014,28 (21):1676-1678,1684.
[9] 谭浩蕾,朱刚才,佘笠,等.MiR-22经上皮-间质转化调控头颈鳞癌的侵袭转移[J].中国耳鼻咽喉颅底外科杂志,2017,23 (1):43-48.Tan HL,Zhu GC,She L,et al.MiR-22 regulates the invasion and metastasis via remodeling epithlial-mesenchymal transition in head and neck squamous cell carcinoma[J].Chin J Otorhinolaryngol Skull Base Surg,2017,23 (1):43-48.
[10] 杨年婷,刘超,李果,等.miR- 324-3p经上皮-间质转化调控鼻咽癌侵袭转移的实验分析[J].中国耳鼻咽喉颅底外科杂志,2016,22 (2):95-99.Yang NT,Liu C,Li G,et al.miR- 324-3p regulates the migration and invasion of nasopharyngeal carcinoma via epithlial-mesenchymal transition[J].Chin J Otorhinolaryngol Skull Base Surg,2016,22 (2):95-99.
[11] Schmittgen TD,Livak KJ.Analyzing real-time PCR data by the comparative C(T) method[J].Nat Protoc,2008,3 (6):1101-1108.
[12] Ferris RL,Blumenschein G Jr.,Fayette J,et al.Nivolumab for recurrent squamous-cell carcinoma of the head and neck [J].N Engl J Med,2016,375(19):1856-1867.
[13] Wellenstein MD,de Visser KE.Cancer-cell-intrinsic mechanisms shaping the tumor immune landscape[J].Immunity,2018,48(3):399-416.
[14] Anaya-Ruiz M,Bandala C,Perez-Santos JL.miR- 485 acts as a tumor suppressor by inhibiting cell growth and migration in breast carcinoma T47D cells[J].Asian Pac J Cancer Prev,2013,14(6):3757-3760.
[15] Kang M,Ren MP,Zhao L,et al.miR- 485-5p acts as a negative regulator in gastric cancer progression by targeting flotillin-1[J].Am J Transl Res,2015,7 (11):2212-2222.
[16] Duan J,Zhang H,Li S,et al.The role of miR- 485-5p/NUDT1 axis in gastric cancer[J].Cancer Cell Int,2017,17:92.
[17] Jiang P,Xu C,Chen L,et al.Epigallocatechin-3-gallate inhibited cancer stem cell-like properties by targeting hsa-mir- 485-5p/RXRalpha in lung cancer[J].J Cell Biochem,2018,119(10):8623-8635.
[18] Sun X,Liu Y,Li M,et al.Involvement of miR-485-5p in hepatocellular carcinoma progression targeting EMMPRIN[J].Biomed Pharmacother,2015,72:58-65.
[19] Lin XJ,He CL,Sun T,et al.hsa-miR-485-5p reverses epithelial to mesenchymal transition and promotes cisplatin-induced cell death by targeting PAK1 in oral tongue squamous cell carcinoma[J].Int J Mol Med,2017,40 (1):83-89.
[20] Zhang SY,Gao F,Peng CG,et al.miR-485-5p promotes osteoporosis via targeting Osterix[J].Eur Rev Med Pharmacol Sci,2018,22 (15):4792-4799.
[21] Hu XX,Xu XN,He BS,et al.microRNA-485-5p Functions as a Tumor Suppressor in Colorectal Cancer Cells by Targeting CD147[J].J Cancer,2018,9 (15):2603-2611.
[22] Wang R,Zuo X,Wang K,et al.MicroRNA-485-5p attenuates cell proliferation in glioma by directly targeting paired box 3[J].Am J Cancer Res,2018,8(12):2507-2517.
[23] Jing LL,Mo XM.Reduced miR-485-5p expression predicts poor prognosis in patients with gastric cancer[J].Eur Rev Med Pharmacol Sci,2016,20(8):1516-1520.