芦笋种质资源ISSR分析
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摘要
基于ISSR对46份芦笋种质资源进行遗传多样性分析。结果表明:21条引物共扩增出多态性条带227条,平均多态位点比率为82.01%,基因多样性指数(H)为0.243,Shannon信息指数(I)为0.377。遗传相似性系数介于0.60~0.97之间,种质间遗传多样性较为丰富。基于ISSR的聚类分析,将46份芦笋种质分为Pacific Challenger 2、A和B共3大类;而A类又分为purple passion、pacific purple和A1等3个亚类,A1种质是由主要来源于Walker Seed、Limseed、新西兰、加拿大和江西而形成一个紧凑的亚类。同样地,B类也分为Jingpurple No.2、NJ1189、Pacific2000和B1共4个亚类,B1种质主要来源于江西、山东、北京、荷兰、美国和意大利。此结果将促进芦笋种质的优化运用,为芦笋遗传改良提供理论基础。
An evaluation of the genetic diversity of 46 asparagus accessions was carried out based on inter-simple sequence repeat(ISSR)markers. The results showed that 21 ISSR primers produced 227 polymorphic bands across the 46 accessions. The values of polymorphic loci percentage, h and I was 82.01%, 0.243 and 0.377, respectively. A cluster analysis was performed based on genetic similarity coefficients(GSCs) which ranged between 0.60 and 0.97 across the 46 accessions. The GSC results showed that abundant genetic diversity existed in the 46 accessions. There were 100 pairs germplasms with relatively strong heterosis. Based on the ISSR cluster analysis, the 46 asparagus accessions were clustered into Pacific Challenger 2, A and B Group A was consisted of Purple passion, Pacific purple and A1 which forming a compact group. And group B was comprised Jingpurple No.2, NJ1189, Pacific 2000 and B1. Group A1 was from Walker Seed, Limseed, New Zealand, Canada and Jiangxi, whereas group B1 Was from Jiangxi, Shandong, Beijing, Netherlands, America and Italy. The results would facilitate the optimal application of asparagus germplasm resources and provide an additional data for genetic improvement.
An evaluation of the genetic diversity of 46 asparagus accessions was carried out based on inter-simple sequence repeat(ISSR)markers. The results showed that 21 ISSR primers produced 227 polymorphic bands across the 46 accessions. The values of polymorphic loci percentage, h and I was 82.01%, 0.243 and 0.377, respectively. A cluster analysis was performed based on genetic similarity coefficients(GSCs) which ranged between 0.60 and 0.97 across the 46 accessions. The GSC results showed that abundant genetic diversity existed in the 46 accessions. There were 100 pairs germplasms with relatively strong heterosis. Based on the ISSR cluster analysis, the 46 asparagus accessions were clustered into Pacific Challenger 2, A and B Group A was consisted of Purple passion, Pacific purple and A1 which forming a compact group. And group B was comprised Jingpurple No.2, NJ1189, Pacific 2000 and B1. Group A1 was from Walker Seed, Limseed, New Zealand, Canada and Jiangxi, whereas group B1 Was from Jiangxi, Shandong, Beijing, Netherlands, America and Italy. The results would facilitate the optimal application of asparagus germplasm resources and provide an additional data for genetic improvement.
引文
[1]刘庭玉.芦笋栽培及营养成分分析[D].吉林:长春理工大学,2009.
[2]陈光宇.中国芦笋研究与产业发展[M].北京:中国农业出版社,2010:3-9.
[3]李索珍.芦笋不同色泽和部位嫩茎的化学成分分析[J].浙江农业学报,1994(1):54-57
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[5]范睿,邬华松,郝朝运,等.胡椒资源ISSR遗传多样性研究[J].热带作物学报,2014,35(1):98-103.
[6]李可峰,韩太利,董贵俊,等.用形态与分子标记研究石刁柏种质资源遗传多样性[J].植物遗传资源学报,2006,7(1):59-65.
[7]李霞,刁家连,李书华,等.芦笋种质资源遗传多样性的RAPD分析[J].植物遗传资源学报,2005,6(3):310-314.
[8]代真真,江程记,杨峰,等.24份芦笋种质的ISSR分析[J].热带农业科学,2012,32(5):35-40.
[9]高建明,代真真,江程记,等.芦笋DNA提取方法的比较研究[J].热带生物学报,2011,2(2):153-156.
[10]陈河龙,张世清,高建明,等.芦笋种质资源形态多样性分析[J].热带农业科学,2016,36(5):55-59.
[11]Innark P.,Ratanachan T.,Khanobdee C.,et al.Downy mildew resistant/susceptible cucumber germplasm(cucumis sativus L.)genetic diversity assessment using ISSR markers[J].Crop Protection,2014,60(6):56-61.
[12]Casali P E,Mercati F,Falavigna A,et al.Genetic characterization of asparagus doubled haploids collection and wild relatives[J].Scientia Horticulturae,2011,130(4):691-700.
[13]Castro P,Gil J,Cabrera A,et al.Assessment of genetic diversity and phylogenetic relationships in Asparagus species related to Asparagus officinalis[J].Genetic Resources and Crop Evolution,2013,60(4):1 275-1 288.
[14]Wang Z,Liao L,Yuan X,et al.Genetic diversity analysis of Cynodon dactylon(bermudagrass)accessions and cultivars from different countries based on ISSR and SSR markers[J].Biochemical Systematics&Ecology,2013,46(2):108-115.
[15]Shao Q S,Guo Q S,Deng Y M,et al.A comparative analysis of genetic diversity in medicinal chrysanthemum morifolium based on morphology,ISSR and SRAP markers[J].Biochemical Systematics&Ecology,2010,38(6):1 160-1 169.
[16]Zhang C S,Xing F G,Selvaraj J N,et al.The effectiveness of ISSR profiling for studying genetic diversity of Aspergillus flavus,from peanut-cropped soils in China[J].Biochemical Systematics&Ecology,2013,50(2):147-153.
[17]Marco C,Clairet F,Mikeall R.EST-SSR markers for asparagus genetic diversity evaluation and cultivar identification[J].Molecular Breeding,2008,21(2),195-204.
[18]Castro P,Rubio J,Gil J,et al.Introgression of new germplasm in current diploid cultivars of garden asparagus from a tetraploid spanish landrace“Morado de Huétor”[J].Scientia Horticulturae,2014,168(168):157-160.
[19]Sarabi B,Hassandokht M R,Hassani M E,et al.Evaluation of genetic diversity among some Iranian wild asparagus populations using morphological characteristics and RAPD markers[J].Scientia Horticulturae,2010,126(1):1-7.
[20]Casali P E,Mercati F,Falavigna A,et al.Genetic characterization of asparagus doubled haploids collection and wild relatives[J].Scientia Horticulturae,2011,130(4):691-700.
[21]Khandka D K.,Nejidat A,Golan-Goldhirsh A.Polymorphism and DNA markers for asparagus cultivars identified by random amplified polymorphic DNA[J].Euphytica,1996,87(1),39-44.
[22]El-Bakatoushi R,Alframawy A M,Samer M,et al.Evolution of the Portulaca oleracea L.aggregate in Egypt on molecular and phenotypic levels revealed by morphology,inter-simple sequence repeat(ISSR)and 18S r DNA gene sequence markers[J].Flora-Morphology,Distribution,Functional Ecology of Plants,2013,208(7):464-477.
[23]Solouki M.,Mehdikhani H.,Zeinali H,et al.Study of genetic diversity in chamomile(Matricaria chamomilla)based on morphological traits and molecular markers[J].Sci.Hortic.2008,117,281-287.
[24]Papadopoulou K.,Ehaliotis C.,Tourna M.,et al.Genetic relatedness among dioecious(Ficus carica L.)cultivars by random amplified polymorphic DNA analysis,and evaluation of agronomic and morphological characters[J].Genetica,2002,114:183-194.
[25]Almajali D,Abdel-Ghani A H,Migdadi H.Evaluation of genetic diversity among Jordanian fig germplasm accessions by morphological traits and ISSR markers[J].Scientia Horticulturae,2012,147(4):8-19.
[26]Farajpour M,Ebrahimi M,Amiri R,et al.Assessment of genetic diversity in Achillea millefolium,accessions from Iran using ISSR marker[J].Biochemical Systematics&Ecology,1978,43(8):73-
[2]陈光宇.中国芦笋研究与产业发展[M].北京:中国农业出版社,2010:3-9.
[3]李索珍.芦笋不同色泽和部位嫩茎的化学成分分析[J].浙江农业学报,1994(1):54-57
[4]刘建乐,白昌军,严琳玲,等.割手密种质资源遗传多样性的ISSR分析[J].热带作物学报,2014,35(1):68-73.
[5]范睿,邬华松,郝朝运,等.胡椒资源ISSR遗传多样性研究[J].热带作物学报,2014,35(1):98-103.
[6]李可峰,韩太利,董贵俊,等.用形态与分子标记研究石刁柏种质资源遗传多样性[J].植物遗传资源学报,2006,7(1):59-65.
[7]李霞,刁家连,李书华,等.芦笋种质资源遗传多样性的RAPD分析[J].植物遗传资源学报,2005,6(3):310-314.
[8]代真真,江程记,杨峰,等.24份芦笋种质的ISSR分析[J].热带农业科学,2012,32(5):35-40.
[9]高建明,代真真,江程记,等.芦笋DNA提取方法的比较研究[J].热带生物学报,2011,2(2):153-156.
[10]陈河龙,张世清,高建明,等.芦笋种质资源形态多样性分析[J].热带农业科学,2016,36(5):55-59.
[11]Innark P.,Ratanachan T.,Khanobdee C.,et al.Downy mildew resistant/susceptible cucumber germplasm(cucumis sativus L.)genetic diversity assessment using ISSR markers[J].Crop Protection,2014,60(6):56-61.
[12]Casali P E,Mercati F,Falavigna A,et al.Genetic characterization of asparagus doubled haploids collection and wild relatives[J].Scientia Horticulturae,2011,130(4):691-700.
[13]Castro P,Gil J,Cabrera A,et al.Assessment of genetic diversity and phylogenetic relationships in Asparagus species related to Asparagus officinalis[J].Genetic Resources and Crop Evolution,2013,60(4):1 275-1 288.
[14]Wang Z,Liao L,Yuan X,et al.Genetic diversity analysis of Cynodon dactylon(bermudagrass)accessions and cultivars from different countries based on ISSR and SSR markers[J].Biochemical Systematics&Ecology,2013,46(2):108-115.
[15]Shao Q S,Guo Q S,Deng Y M,et al.A comparative analysis of genetic diversity in medicinal chrysanthemum morifolium based on morphology,ISSR and SRAP markers[J].Biochemical Systematics&Ecology,2010,38(6):1 160-1 169.
[16]Zhang C S,Xing F G,Selvaraj J N,et al.The effectiveness of ISSR profiling for studying genetic diversity of Aspergillus flavus,from peanut-cropped soils in China[J].Biochemical Systematics&Ecology,2013,50(2):147-153.
[17]Marco C,Clairet F,Mikeall R.EST-SSR markers for asparagus genetic diversity evaluation and cultivar identification[J].Molecular Breeding,2008,21(2),195-204.
[18]Castro P,Rubio J,Gil J,et al.Introgression of new germplasm in current diploid cultivars of garden asparagus from a tetraploid spanish landrace“Morado de Huétor”[J].Scientia Horticulturae,2014,168(168):157-160.
[19]Sarabi B,Hassandokht M R,Hassani M E,et al.Evaluation of genetic diversity among some Iranian wild asparagus populations using morphological characteristics and RAPD markers[J].Scientia Horticulturae,2010,126(1):1-7.
[20]Casali P E,Mercati F,Falavigna A,et al.Genetic characterization of asparagus doubled haploids collection and wild relatives[J].Scientia Horticulturae,2011,130(4):691-700.
[21]Khandka D K.,Nejidat A,Golan-Goldhirsh A.Polymorphism and DNA markers for asparagus cultivars identified by random amplified polymorphic DNA[J].Euphytica,1996,87(1),39-44.
[22]El-Bakatoushi R,Alframawy A M,Samer M,et al.Evolution of the Portulaca oleracea L.aggregate in Egypt on molecular and phenotypic levels revealed by morphology,inter-simple sequence repeat(ISSR)and 18S r DNA gene sequence markers[J].Flora-Morphology,Distribution,Functional Ecology of Plants,2013,208(7):464-477.
[23]Solouki M.,Mehdikhani H.,Zeinali H,et al.Study of genetic diversity in chamomile(Matricaria chamomilla)based on morphological traits and molecular markers[J].Sci.Hortic.2008,117,281-287.
[24]Papadopoulou K.,Ehaliotis C.,Tourna M.,et al.Genetic relatedness among dioecious(Ficus carica L.)cultivars by random amplified polymorphic DNA analysis,and evaluation of agronomic and morphological characters[J].Genetica,2002,114:183-194.
[25]Almajali D,Abdel-Ghani A H,Migdadi H.Evaluation of genetic diversity among Jordanian fig germplasm accessions by morphological traits and ISSR markers[J].Scientia Horticulturae,2012,147(4):8-19.
[26]Farajpour M,Ebrahimi M,Amiri R,et al.Assessment of genetic diversity in Achillea millefolium,accessions from Iran using ISSR marker[J].Biochemical Systematics&Ecology,1978,43(8):73-