人参茎叶中人参皂苷的提取、分离纯化及其药理作用的研究进展
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摘要
人参茎叶为五加科植物人参的干燥茎叶,主要活性成分为人参皂苷,具有补气,生津,解暑,抗疲劳等功效.本文对人参茎叶中人参皂苷的提取方法、分离纯化和药理作用进行了综述,为研究人参茎叶皂苷的提取及应用提供了一定的理论依据.
Ginseng stem and leaf is dry leaves of five plus families ginseng plant,the main active ingredient of ginseng stem and leaf was ginsenoside,which has the advantages of engendering liquid,resolving summer heat,anti-fatigue action and so on. The traditional extraction methods include water extraction,alcohol extraction,etc. In this paper,the extraction and separation method of ginsenosides in ginseng stem and leaf,pharmacological activities were reviewed which provides a theoretical basis for the study of Ginsenoide from Ginseng stem and leaf.
Ginseng stem and leaf is dry leaves of five plus families ginseng plant,the main active ingredient of ginseng stem and leaf was ginsenoside,which has the advantages of engendering liquid,resolving summer heat,anti-fatigue action and so on. The traditional extraction methods include water extraction,alcohol extraction,etc. In this paper,the extraction and separation method of ginsenosides in ginseng stem and leaf,pharmacological activities were reviewed which provides a theoretical basis for the study of Ginsenoide from Ginseng stem and leaf.
引文
[1]张黎黎,王栋.水提取人参茎叶皂苷最佳工艺的研究[J].云南中医中药杂志,2008,29(3):48-49.
[2]Hyun Jung Lee,Hyun-Sun Lee,Hye Jin Cho,et,al.Utilization of hydrolytic enzymes for the extraction of ginsenosides from Korean ginseng leaves[J].Process Biochemistry.2012,47:538-543.
[3]辛海量,盛佳钰,葛凌弦,等.人参叶提取物的制备工艺研究[J].医药导报,2011,30(1):79-82.
[4]于杰,白志川,付超美.人参茎叶总皂甙提取精制工艺研究[J].西南农业大学学报:自然科学版,2004,26(4):479-482.
[5]马玉婷,李进,陈敏,等.酶解法提取黑果枸杞酒渣花色苷的工艺研究[J].食品科技,2011,36(7):182-186.
[6]伍毅,王洪新,朱松.β-葡萄糖苷酶法与盐酸法水解银杏黄酮制备黄酮苷元的比较[J].食品科学,2008,29(7):146-150.
[7]吴清,倪宁,葛凌弦,等.星点设计-效应面法优选人参叶酶法提取工艺[J].中国实验方剂学杂志,2006,12(4):5-8.
[8]石任兵,李祥.中药化学[M].北京:科学出版社,2005.
[9]季晓晖.超声波法提取西洋参茎叶中人参皂苷Re工艺的优化[J].湖北农业科学,2013,52(13):3137-3139,3177.
[10]Xiaolei Shi,Yongri Jin,Jingbo Liu,et al.Matrix solid phase dispersion extraction of ginsenosides in the leaves of Panax ginseng C.M.Mey[J].Food Chemistry.2011,4(67):1253-1257.
[11]曾庆荣,刘放,张振坤.从人参茎叶中提取人参皂甙的研究[J].化工时刊,2009,23(6):26-28.
[12]周如金,黄敏,张玲,等.大孔吸附树脂提取甜茶苷的研究[J].林产化学与工业,2008,28(5):35-39.
[13]谢丽玲,任理,赖县生,等.红参中人参总皂苷的提取纯化工艺研究[J].中药材,2009,32(10):1602-1605.
[14]彭拓华,陈洁炜,黄湘兰,等.大孔树脂富集纯化人参总皂苷工艺条件优选[J].中药材,2006,29(4):392-394.
[15]姚海燕,万玉华,沈雅婕,等.大孔吸附树脂纯化人参总皂苷的工艺研究[J].环球中医药,2013,6(2):84-88.
[16]唐晓慧.人参叶的化学成分研究[D].沈阳:沈阳药科大学,2008.
[17]程慧,宋新波,张丽娟.人参皂苷Rg3与Rh2的研究进展[J].药物评价研究,2010,33(4):307-311.
[18]J.Wanga,G.Zuob,J.Lia,T.Guan,et,al.Induction of tumoricidal activity in mouse peritoneal macrophages by ginseng polysaccharide[J].International Journal of Biological Macromolecules.2010,2(7):389-395.
[19]张晓文,宋清,徐志伟.人参茎叶总皂苷对心理应激荷瘤小鼠肿瘤生长及化疗效果的影响[J].现代食品科技,2010,26(4):348-350.
[20]Lijuan Zhai,Yutao Li,Weiyu Wang.Effect of oral administration of ginseng stem-and-leaf saponins(GSLS)on the immune responses to Newcastle disease vaccine in chickens[J].Vaccine.2011,97(4):5007-5014.
[21]陈宏硕,王维君,张国蓉,等.三种软骨多糖清除自由基活性研究[J].食品研究与开发,2008,29(2):56-59.
[22]王双珠,曾勇,李燕,等.阿克苷神经保护机制研究进展[J].医学研究杂志,2011,40(11):6-8.
[23]Thiyagarajan Ramesha,Sung-Won Kima,Seock-Yeon Hwang,et,al.Panax ginseng reduces oxidative stress and restores antioxidant capacity in aged rats[J].SciVerse Science Direct.2012,8(5):718-726.
[24]Hyeong-Geug Kim,Sa-Ra Yoo,Hye-Jung Park,et,al.Antioxidant effects of Panax ginseng C.A.Meyer in healthy subjects:A randomized,placebo-controlled clinical trial[J].Food and Chemical Toxicology.2011,20(6):2229-2235.
[25]惠汝太,樊晓寒.新的心脑血管病危险因素[J].临床内科杂志,2005,22(1):1-3.
[26]Rui Liua,JingZhao Zhangb,WenCong Liu,et,al.AntiObesity effects of protopanaxdiol types of Ginsenosides isolated from the leaves of American ginseng(Panax quinquefolius L.)in mice fed with a high-fat diet[J].Fitoterapia.2010,7(2):1079-1087.
[27]Souren Paul,Heung Sop Shin,Sun Chul Kang.Inhibition of inflammations and macrophage activation by ginsenoside-Re isolated from Korean ginseng(Panax ginseng C.A.Meyer)[J].Food and Chemical Toxicology.2012,35(2):1354-1361.
[28]姜淼,黄欣,沈亮亮,等.2种中药单体对念珠菌的体外药敏研究[J].中国真菌学杂志,2011,6(1):26-30.
[29]李凤林,吕红英,王广耀,等.人参茎叶皂苷对体力疲劳缓解作用的研究[J].饮料工业,2013,16(6):21-23.
[30]Chee Ping Fadzel Wong,Amit Bandyopadhyay,Chee Keong Chen.Effects of Panax ginseng supplementation on physiology responses during endurance performance[J].Journal of Men's Health.2011,8(1):78-80.
[31]彭彬,王朝丽,冯丽,等.人参皂苷Rg1调控神经干细胞衰老作用及机制探讨[J].中国细胞生物学学报,2011,33(10):1116-1122.
[32]李凤林.人参茎叶皂苷药理作用的研究进展[J].贵州农业科学,2013,41(2):54-57.
[33]程俊霖,周黎明,朱玲,等.人参茎叶总皂苷对衰老小鼠的作用研究[J].四川生理科学杂志,2004,26(3):97-99.
[34]Hanumantha Rao Balaji Raghavendrana,Sathyanath Rekhaa,Hyeong-Keug Kim Jung-Hyo Cho,et,al.Ginsenoside rich fraction of Panax ginseng C.A.Meyer improve feeding behavior following radiation-induced pica in rats[J].Fitoterapia,2012,4(8):1144-1150.
[35]唐泽耀,唐田田,付雷,等.人参茎叶皂苷对实验性小鼠心电改变及死亡时间的影响[J].实验动物科学,2009,26(4):4-7.
[36]Hyun Jeong Hana,Hye Yun Kima,et,al.Effects of red ginseng extract on sleeping behaviors in human volunteers[J].Journal of Ethnopharmacology.2013,7(5):597-599.
[37]刘菲,赵文杰,刘南,等.人参茎叶皂苷对毛囊间充质干细胞增殖的影响[J].黑龙江医药,2012,25(2):232-234.
[2]Hyun Jung Lee,Hyun-Sun Lee,Hye Jin Cho,et,al.Utilization of hydrolytic enzymes for the extraction of ginsenosides from Korean ginseng leaves[J].Process Biochemistry.2012,47:538-543.
[3]辛海量,盛佳钰,葛凌弦,等.人参叶提取物的制备工艺研究[J].医药导报,2011,30(1):79-82.
[4]于杰,白志川,付超美.人参茎叶总皂甙提取精制工艺研究[J].西南农业大学学报:自然科学版,2004,26(4):479-482.
[5]马玉婷,李进,陈敏,等.酶解法提取黑果枸杞酒渣花色苷的工艺研究[J].食品科技,2011,36(7):182-186.
[6]伍毅,王洪新,朱松.β-葡萄糖苷酶法与盐酸法水解银杏黄酮制备黄酮苷元的比较[J].食品科学,2008,29(7):146-150.
[7]吴清,倪宁,葛凌弦,等.星点设计-效应面法优选人参叶酶法提取工艺[J].中国实验方剂学杂志,2006,12(4):5-8.
[8]石任兵,李祥.中药化学[M].北京:科学出版社,2005.
[9]季晓晖.超声波法提取西洋参茎叶中人参皂苷Re工艺的优化[J].湖北农业科学,2013,52(13):3137-3139,3177.
[10]Xiaolei Shi,Yongri Jin,Jingbo Liu,et al.Matrix solid phase dispersion extraction of ginsenosides in the leaves of Panax ginseng C.M.Mey[J].Food Chemistry.2011,4(67):1253-1257.
[11]曾庆荣,刘放,张振坤.从人参茎叶中提取人参皂甙的研究[J].化工时刊,2009,23(6):26-28.
[12]周如金,黄敏,张玲,等.大孔吸附树脂提取甜茶苷的研究[J].林产化学与工业,2008,28(5):35-39.
[13]谢丽玲,任理,赖县生,等.红参中人参总皂苷的提取纯化工艺研究[J].中药材,2009,32(10):1602-1605.
[14]彭拓华,陈洁炜,黄湘兰,等.大孔树脂富集纯化人参总皂苷工艺条件优选[J].中药材,2006,29(4):392-394.
[15]姚海燕,万玉华,沈雅婕,等.大孔吸附树脂纯化人参总皂苷的工艺研究[J].环球中医药,2013,6(2):84-88.
[16]唐晓慧.人参叶的化学成分研究[D].沈阳:沈阳药科大学,2008.
[17]程慧,宋新波,张丽娟.人参皂苷Rg3与Rh2的研究进展[J].药物评价研究,2010,33(4):307-311.
[18]J.Wanga,G.Zuob,J.Lia,T.Guan,et,al.Induction of tumoricidal activity in mouse peritoneal macrophages by ginseng polysaccharide[J].International Journal of Biological Macromolecules.2010,2(7):389-395.
[19]张晓文,宋清,徐志伟.人参茎叶总皂苷对心理应激荷瘤小鼠肿瘤生长及化疗效果的影响[J].现代食品科技,2010,26(4):348-350.
[20]Lijuan Zhai,Yutao Li,Weiyu Wang.Effect of oral administration of ginseng stem-and-leaf saponins(GSLS)on the immune responses to Newcastle disease vaccine in chickens[J].Vaccine.2011,97(4):5007-5014.
[21]陈宏硕,王维君,张国蓉,等.三种软骨多糖清除自由基活性研究[J].食品研究与开发,2008,29(2):56-59.
[22]王双珠,曾勇,李燕,等.阿克苷神经保护机制研究进展[J].医学研究杂志,2011,40(11):6-8.
[23]Thiyagarajan Ramesha,Sung-Won Kima,Seock-Yeon Hwang,et,al.Panax ginseng reduces oxidative stress and restores antioxidant capacity in aged rats[J].SciVerse Science Direct.2012,8(5):718-726.
[24]Hyeong-Geug Kim,Sa-Ra Yoo,Hye-Jung Park,et,al.Antioxidant effects of Panax ginseng C.A.Meyer in healthy subjects:A randomized,placebo-controlled clinical trial[J].Food and Chemical Toxicology.2011,20(6):2229-2235.
[25]惠汝太,樊晓寒.新的心脑血管病危险因素[J].临床内科杂志,2005,22(1):1-3.
[26]Rui Liua,JingZhao Zhangb,WenCong Liu,et,al.AntiObesity effects of protopanaxdiol types of Ginsenosides isolated from the leaves of American ginseng(Panax quinquefolius L.)in mice fed with a high-fat diet[J].Fitoterapia.2010,7(2):1079-1087.
[27]Souren Paul,Heung Sop Shin,Sun Chul Kang.Inhibition of inflammations and macrophage activation by ginsenoside-Re isolated from Korean ginseng(Panax ginseng C.A.Meyer)[J].Food and Chemical Toxicology.2012,35(2):1354-1361.
[28]姜淼,黄欣,沈亮亮,等.2种中药单体对念珠菌的体外药敏研究[J].中国真菌学杂志,2011,6(1):26-30.
[29]李凤林,吕红英,王广耀,等.人参茎叶皂苷对体力疲劳缓解作用的研究[J].饮料工业,2013,16(6):21-23.
[30]Chee Ping Fadzel Wong,Amit Bandyopadhyay,Chee Keong Chen.Effects of Panax ginseng supplementation on physiology responses during endurance performance[J].Journal of Men's Health.2011,8(1):78-80.
[31]彭彬,王朝丽,冯丽,等.人参皂苷Rg1调控神经干细胞衰老作用及机制探讨[J].中国细胞生物学学报,2011,33(10):1116-1122.
[32]李凤林.人参茎叶皂苷药理作用的研究进展[J].贵州农业科学,2013,41(2):54-57.
[33]程俊霖,周黎明,朱玲,等.人参茎叶总皂苷对衰老小鼠的作用研究[J].四川生理科学杂志,2004,26(3):97-99.
[34]Hanumantha Rao Balaji Raghavendrana,Sathyanath Rekhaa,Hyeong-Keug Kim Jung-Hyo Cho,et,al.Ginsenoside rich fraction of Panax ginseng C.A.Meyer improve feeding behavior following radiation-induced pica in rats[J].Fitoterapia,2012,4(8):1144-1150.
[35]唐泽耀,唐田田,付雷,等.人参茎叶皂苷对实验性小鼠心电改变及死亡时间的影响[J].实验动物科学,2009,26(4):4-7.
[36]Hyun Jeong Hana,Hye Yun Kima,et,al.Effects of red ginseng extract on sleeping behaviors in human volunteers[J].Journal of Ethnopharmacology.2013,7(5):597-599.
[37]刘菲,赵文杰,刘南,等.人参茎叶皂苷对毛囊间充质干细胞增殖的影响[J].黑龙江医药,2012,25(2):232-234.