离子交换纤维对三七总皂苷动态吸附和解吸的研究
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摘要
本文分别考察了流速、pH值、上柱液体积和浓度对强碱性阴离子交换纤维动态吸附和解吸三七总皂苷的影响,并计算了动态吸附和解吸活化能.结果表明:在初始质量浓度为0.552 mg/mL、体积为35 mL、pH=7、流速为0.5 mL/min时吸附效果最佳,吸附率为84.19%,动态吸附活化能为21.04 kJ/mol;而在解吸剂体积为80 mL/0.100 0 g饱和纤维,pH=1的条件下,用60%的乙醇水溶液作为解吸剂,解吸效果最佳,解吸率为88.41%,动态解吸活化能为30.82 kJ/mol.这表明用离子交换纤维提取纯化三七总皂苷的方法是可行的,这为皂苷的分离提纯开辟了新的途径.
The paper investigated the influence of velocity,pH value,concentration and volume of the saponins solution on dynamic adsorption and desorption of total saponins of panax notoginseng by strong alkalinity ion-exchange fiber,respectively,and the dynamic adsorption and desorption activation energy were got too.The results showed that the optimum conditions for adsorption was as the following: initial concentration was 0.552 mg/mL,pH value was 7,the volume was 35 mL and the velocity was 0.5 mL/min.The adsorption rate was 84.19% and the dynamic adsorption activation energy was 21.04 kJ/mol.The optimum conditions for desorption was as the following: the desorption agent was ethanol solution which volume fraction was 60%,pH value was 1 and the volume of the desorption agent was 80 mL /0.100 0 g saturated fiber.The desorption rate was 88.41% and the dynamic desorption activation energy was 30.82 kJ/mol.The method of using ion exchange fiber to extract and refine total saponins of panax notoginseng is feasible.It opens up a new channel for extracting and refining saponins.
The paper investigated the influence of velocity,pH value,concentration and volume of the saponins solution on dynamic adsorption and desorption of total saponins of panax notoginseng by strong alkalinity ion-exchange fiber,respectively,and the dynamic adsorption and desorption activation energy were got too.The results showed that the optimum conditions for adsorption was as the following: initial concentration was 0.552 mg/mL,pH value was 7,the volume was 35 mL and the velocity was 0.5 mL/min.The adsorption rate was 84.19% and the dynamic adsorption activation energy was 21.04 kJ/mol.The optimum conditions for desorption was as the following: the desorption agent was ethanol solution which volume fraction was 60%,pH value was 1 and the volume of the desorption agent was 80 mL /0.100 0 g saturated fiber.The desorption rate was 88.41% and the dynamic desorption activation energy was 30.82 kJ/mol.The method of using ion exchange fiber to extract and refine total saponins of panax notoginseng is feasible.It opens up a new channel for extracting and refining saponins.
引文
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[7]郑义,陆辉,杜改梅.利用大孔树脂吸附法分离纯化三七总皂苷的工艺研究[J].金陵科技学院学报,2008,24(1):76-77.
[8]郭嘉,陈延林,罗晔,等.新型离子交换纤维的应用研究与展望[J].高技术纤维与应用,2005,30(6):35-38.
[9]刘廷岳,聂素双,蒋亮,等.离子交换纤维对葛根素动态吸附和解吸的考察[J].中国新药杂志,2007,16(6):458-461.
[10]杨海燕,冯长根,曾庆轩.强酸离子交换纤维对碱性氨基酸的吸附性能研究[J].化工进展,2004,23(7):740-742.
[11]石召华,熊富良,李崇明,等.大孔吸附树脂分离纯化三七总皂苷工艺研究[J].中成药,2004,26(1):10-12.
[12]天津大学物理化学教研室.物理化学(第五版下册)[M].北京:高等教育出版社,2009:529-534.
[2]郑明.三七总皂苷分离纯化工艺研究[J].海峡药学,2010,22(2):15-16.
[3]郑明,霍林海,楼宜嘉.三七总皂苷分离纯化工艺研究[J].中国现代应用药学志,2007,24(2):118-120.
[4]刘中秋,蔡雄,赖小平,等.大孔吸附树脂富集纯化三七总皂苷工艺研究[J].中国实验方剂学杂志,2001,7(3):4-6.
[5]孙剑平,崔淑霞,杨静伟.大孔吸附树脂富集三七总皂苷的工艺研究[J].黑龙江医药,2007,20(3):232-233.
[6]彭拓华,陈洁炜,黄湘兰,等.大孔树脂富集纯化人参总皂苷工艺条件优选[J].中药材,2006,29(4):392-394.
[7]郑义,陆辉,杜改梅.利用大孔树脂吸附法分离纯化三七总皂苷的工艺研究[J].金陵科技学院学报,2008,24(1):76-77.
[8]郭嘉,陈延林,罗晔,等.新型离子交换纤维的应用研究与展望[J].高技术纤维与应用,2005,30(6):35-38.
[9]刘廷岳,聂素双,蒋亮,等.离子交换纤维对葛根素动态吸附和解吸的考察[J].中国新药杂志,2007,16(6):458-461.
[10]杨海燕,冯长根,曾庆轩.强酸离子交换纤维对碱性氨基酸的吸附性能研究[J].化工进展,2004,23(7):740-742.
[11]石召华,熊富良,李崇明,等.大孔吸附树脂分离纯化三七总皂苷工艺研究[J].中成药,2004,26(1):10-12.
[12]天津大学物理化学教研室.物理化学(第五版下册)[M].北京:高等教育出版社,2009:529-534.