摘要
内源性植物激素是在植物体中合成,并在整个植物生命周期内调控其生长发育、应对外界刺激等过程中发挥重要作用的一类微量或痕量有机小分子化合物。随着植物激素分析方法的发展,分析样品用量逐渐减少,不同植物组织(或器官)中植物激素的种类和含量差异不断呈现,极大促进了植物激素生理作用的研究。近年来,植物样品中内源性植物激素的时空分布研究已成为植物激素分析的一大热点。该文总结了近五年来,内源性植物激素时空分布的研究进展,主要从分析难点、分析方法、主要植物激素在植物体内的时空分布情况等方面进行讨论和总结,相关内容围绕本研究组在植物激素检测方面的工作展开。最后,展望了植物激素时空分析这一研究领域的未来发展方向。
Endogenous phytohormone is a kind of trace organic small molecule compound synthesized in plants. It plays important roles in regulating the growth and development of plants throughout their life cycles, and responding to external stimuli. With the development of analytical methods for the detection of phytohormone, the amount of analytical samples is gradually reduced. Moreover, the differences in the types and contents of phytohormones in different plant tissues(or organs) are constantly presented. These developments greatly promote the study of the physiological effects of plant hormones. In recent years, the temporal and spatial distribution of endogenous phytohormones in several plant samples has attracted more and more attention in plant hormone analysis. This review summarizes the research progress made in the study of the spatial and temporal distribution of endogenous phytohormones in the last five years. Mainly, the review summarizes and discusses the difficulties associated with analysis, the development of analytical methods, and the spatial and temporal distribution of major plant hormones. The sampling methods, sample preparation and detection methods for spatial and temporal distribution studies are discussed in detail with a special focus on our work in plant hormone detection. Finally, the future development of the plant hormone analysis research field analysis is prospected.
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