花椒窄吉丁雌、雄成虫触角差异表达基因的初步筛选
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摘要
花椒窄吉丁(Agrilus zanthoxylumi)是我国北方花椒(Zanthoxylum bungeanum)产区一种重要的毁灭性的蛀干害虫,该昆虫主要通过触角的灵敏性和特异性识别挥发性气味分子,触角对其生存和繁衍起着重要作用。为了筛选出花椒窄吉丁雌、雄成虫触角中差异表达的基因,初步解析花椒窄吉丁触角中与信息素结合功能相关的候选差异基因,本研究选取雌、雄成虫触角为研究对象进行了转录组测序,在获得转录组测序数据后,借助生物信息学软件对其进行分析,筛选出了花椒窄吉丁成虫触角中雌、雄两性间差异表达的基因,并进行相关差异表达基因的功能富集统计。花椒窄吉丁雌、雄成虫触角转录组数据共获得36 209个unigenes,共检测到726个基因有显著表达差异,其中459个基因上调,267个基因下调。通过基因本体(Gene Ontology, GO)富集分析发现,差异基因显著富集于结合功能、催化活性、细胞进程、代谢过程、细胞组分、细胞器。共有472条差异基因在KEGG数据库中得到了注释,对其进行Pathway富集分析表明,共参与到165条代谢途径中,表达上调共394个,表达下调共78个基因。本研究首次利用转录组测序技术对花椒窄吉丁成虫触角活性成分富集的遗传基础进行了分析,在组学层面上分析探讨了成虫触角差异表达的基因,对于揭示基因功能、了解基因间相互关系,进一步完善触角感受外界气味物质的机理起着至关重要的作用,同时也为害虫生物防控技术提供基础资料。
Agrilus zanthoxylumi is a significant and destructive branch-borers pest in the Zanthoxylum bungeanum planting areas of northern China. This insect mainly recognizes volatile odor molecules through sensitivity and specificity via antenna, which plays an important role in its survival and reproduction. To select Agrilus zanthoxylumi adult male and female antenna of differentially expressed genes, preliminary parsing and Agrilus zanthoxylumi candidate differences related to the behavior and the biological prevention and control in terms of seeking mate genes, this study selected adult female and male antenna transcriptome sequencing as the research object, using bioinformatics software to analyze the transcriptome sequencing data obtained and screening of differentially expressed genes between the 2 samples of male and female, further analysis of differentially expressed genes. The results showed that 36 209 unigene were obtained from the antenna transcriptome data of the male and female adults of Agrilus zanthoxylumi. A total of 726 genes were detected with significant differences in expression, among which 459 genes were up-expressed and 267 genes were down-expressed. Through GO(Gene Ontology) enrichment analysis, the differentially expressed genes were found in binding function, catalytic activity, cell process, metabolic process, cell components and organelles which were significantly enriched. A total of 472 differentially expressed genes were annotated in the KEGG database, and Pathway enrichment analysis showed that they were involved in 165 metabolic pathways, with394 up-expressed and 78 down-expressed genes. This study obtained Agrilus zanthoxylumi adult antenna of the genetic basis of enrichment of the active ingredient based on the transcriptome sequencing technologies,discussed the adult male and female antenna differentially expressed genes, revealed the gene function,understood the relationship between genes. It doesn't only further improve the mechanism of tentacles feelings outside odor material plays an important role, but also provide the basic data for pest prevention and control technology.
Agrilus zanthoxylumi is a significant and destructive branch-borers pest in the Zanthoxylum bungeanum planting areas of northern China. This insect mainly recognizes volatile odor molecules through sensitivity and specificity via antenna, which plays an important role in its survival and reproduction. To select Agrilus zanthoxylumi adult male and female antenna of differentially expressed genes, preliminary parsing and Agrilus zanthoxylumi candidate differences related to the behavior and the biological prevention and control in terms of seeking mate genes, this study selected adult female and male antenna transcriptome sequencing as the research object, using bioinformatics software to analyze the transcriptome sequencing data obtained and screening of differentially expressed genes between the 2 samples of male and female, further analysis of differentially expressed genes. The results showed that 36 209 unigene were obtained from the antenna transcriptome data of the male and female adults of Agrilus zanthoxylumi. A total of 726 genes were detected with significant differences in expression, among which 459 genes were up-expressed and 267 genes were down-expressed. Through GO(Gene Ontology) enrichment analysis, the differentially expressed genes were found in binding function, catalytic activity, cell process, metabolic process, cell components and organelles which were significantly enriched. A total of 472 differentially expressed genes were annotated in the KEGG database, and Pathway enrichment analysis showed that they were involved in 165 metabolic pathways, with394 up-expressed and 78 down-expressed genes. This study obtained Agrilus zanthoxylumi adult antenna of the genetic basis of enrichment of the active ingredient based on the transcriptome sequencing technologies,discussed the adult male and female antenna differentially expressed genes, revealed the gene function,understood the relationship between genes. It doesn't only further improve the mechanism of tentacles feelings outside odor material plays an important role, but also provide the basic data for pest prevention and control technology.
引文
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