花生SSR标记与品质性状的相关分析
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摘要
为了研究花生SSR分子标记与品质性状之间的关系,本研究以农家品种四粒红和冀农黑3号杂交产生的251个重组自交系(RIL)群体为材料,运用SPSS17.0软件分析花生RIL群体品质性状与SSR分子标记的相关性。结果表明多数品质性状间有极显著或显著相关的关系。棕榈酸和油酸的相关系数最高为(r=-0.887),其次为硬脂酸和油酸(r=-0.753)。Pearson’s相关分析表明77个分子标记与8个品质性状存在着显著相关性。运用多元回归分析得到45个与8个品质性状相关的标记,解释的表型总变异的范围为8.1%~28.5%。上述结果为利用分子标记辅助选择改良花生的品质性状提供了参考。
In order to study the relationship between SSR markers and quality traits in peanut, 251 recombinant inbred lines(RILs), which derived from a cross between Silihong and Jinonghei 3, In this study, were employed to analyze the correlation relationship between quality traits and SSR markers using SPSS 17.0 software. in this study The results showed that there were significant correlations among 8 quality traits. The palmitic and oleic acid have higher correlation, followed by stearic acid and oleic acid(r =-0.753). Through pearson's correlation analysis, 77 molecular markers(r=-0.887) were significantly correlated with 8 quality traits. Forty five molecular markers were associated with 8 quality traits by stepwise multiple linear regression analysis with explained phenotypic variances of 8.1% ~28.5%. The results are useful for improvement of quality traits by molecular marker-assisted selection.
In order to study the relationship between SSR markers and quality traits in peanut, 251 recombinant inbred lines(RILs), which derived from a cross between Silihong and Jinonghei 3, In this study, were employed to analyze the correlation relationship between quality traits and SSR markers using SPSS 17.0 software. in this study The results showed that there were significant correlations among 8 quality traits. The palmitic and oleic acid have higher correlation, followed by stearic acid and oleic acid(r =-0.753). Through pearson's correlation analysis, 77 molecular markers(r=-0.887) were significantly correlated with 8 quality traits. Forty five molecular markers were associated with 8 quality traits by stepwise multiple linear regression analysis with explained phenotypic variances of 8.1% ~28.5%. The results are useful for improvement of quality traits by molecular marker-assisted selection.
引文
Chen W.G.,Jiao Y.Q.,Cheng L.Q.,Huang L.,Liao B.S.,Tang M.,Ren X.P.,Zhou X.J.,Chen Y.N.,and Jiang H.F.,2016Quantitative trait locus analysis for pod-and kernel-related traits in the cultivated peanut(Arachis hypogaea L.),BMCGenetics,17:25
Cheng L.Q.,Tang M.,Ren X.P.,Huang L.,Chen W.G.,Li Z.D.Zhou X.J.,Chen Y.N.,Liao B.S.,and Jiang H.F.,2015Construction of genetic map and QTL analysis for mainstem height and total branch number in peanut(Arachis hypogae L.),Zuowu Xuebao(Acta Agronomca Sinica),41(6):979-987(成良强,唐梅,任小平,黄莉,陈伟刚,李振动,周小静,陈玉宁,廖伯寿,姜慧芳,2015,栽培种花生遗传图谱的构建及主茎高和总分枝数QTL分析,作物学报,41(6):979-987)
Chu Y.,Wu C.L.,Holbrook C.C.,Tillman B.L.,Person G.,and Ozias-Akins P.,2011,Marker-assisted selection to pyramid n ematode resistance and the high oleic trait in peanut,The Plant Genome,4(2):110-117
Cui S.L.,2010,Studies on the genetic diversity of peanu(Arachis hypogaea L.)landraces in Hebei Province,Thesi for M.S.,Agricultural University of Hebei,Supervisor:Liu L.F.,pp.10(崔顺立,2010,河北省花生(Arachis hypogae L.)地方品种遗传多样性研,硕士学位论文,河北农业大学,导师:刘立峰,pp.10)
Fang X.J.,Wu W.R.,and Tang J.L.,2002,DNA marker assisted in crop breeding,Science Press,Beijing,China,pp.41-45(方宣钧,吴为人,唐纪良,2002,作物DNA标记辅助育种,科学出版社,中国,北京,pp.41-45)
Gao J.R.,Han X.L.,Kong C.L.,Zhang Y.X.,Li A.F.,Ning T.Y.and Wang C.H.,2009,Applications of DA7200 near in frared apparatus in analyzing the quality of wheats,Shiyan shi Kexue(Laboratory Science),(1):173-176(高居荣,韩秀兰,孙彩玲,张永祥,李安飞,宁堂原,王承海,2009DA7200近红外仪在小麦品质分析中的应用研究,实验室科学,(1):173-176)
Guo J.B.,Huang L.,Chen L.Q.,Chen W.G.,Ren X.P.,Chen YN.,Zhou X.J.,Shen J.X.,and Jiang H.F.,2016,An integrat ed genetic linkage map from three F2populations of cultivat ed peanut(Arachis hypogaea L.),Zuowu Xuebao(Acta Agronomica Sinica),42(2):159-169(郭建斌,黄莉,成良强陈伟刚,任小平,陈玉宁,周小静,沈金雄,姜慧芳,2016利用3个F2群体整合高密度栽培种花生遗传连锁图,作物学报,42(2):159-169)
Huang L.,He H.Y.,Chen W.G.,Ren X.P.,Chen Y.N.,Zhou X.J.Xia Y.L.,Wang X.L.,Jiang X.G.,Liao B.S.,and Jiang H.F.2015,Quantitative trait locus analysis of agronomic and quality-related traits in cultivated peanut(Arachis hypogaea L.),Theor.Appl.Genet.,128(6):1103-1115
Khedikar Y.P.,Gowda M.V.C.,Sarvamangala C.,Patgar K.V.Upadhyaya H.D.,and Varshney R.K.,2010,A QTL study on late leaf spot and rust revealed one major QTL for molec ular breeding for rust resistance in groundnut(Arachis hypogaea L.),Theor.Appl.Genet.,121(5):971-984
Li X.P.,Xu Z.J.,Cai Y,Guo J.,B.,Huang L.,Ren X.P.,Li Z.,D.Chen W.G.,Luo H.Y.,Zhou X.J.,Chen Y.N.,Wu M.Y.and Jiang H.F.,2016,Quantitative trait locus analysis fo main quality traits in cultivated peanut(Arachis hypogae L.),Zhongguo Youliao Zuowu Xuebao(Chinese Journal o Oil Crop Sciences),38(4):415-422(李新平,徐志军,蔡岩郭建斌,黄莉,任小平,李振动,陈伟刚,罗怀勇,周小静陈玉宁,吴明煜,姜慧芳,2016,花生主要品质性状的QTL定位分析,中国油料作物学报,38(4):415-422)
Li Z.D.,Li X.P.,Huang L.,Ren X.P.,Chen Y.N.,Zhou X.J.,Liao B.S.,and Jiang H.F.,2015,Mapping of QTLs for pod size related traits in cultivated peanut(Arachis hypogaea L.)Zuowu Xuebao(Acta Agronomia Sinina),41(9):1313-1323(李振动,李新平,黄莉,任小平,陈玉宁,周小静,廖伯寿姜慧芳,2015,栽培种花生荚果大小相关性状QTL定位作物学报,41(9):1313-1323)
Liu L.F.,Dang P.M.,and Chen C.Y.,2015,Development and uti lization of In Del markers to identify peanut(Arachis hypo gaea)disease resistance,Frontiers in Plant Science,6:988
Pandey M.K.,Monyo E.,Ozias-Akins P.,Liang X.Q.,Guimare P.,Nigam S.M.,Upadhyaya H.D.,Janila P.,Zhang X.Y.Guo B.Z.,Cook D.R.,Bertioli D.J.,Michelmore R.,and Varshney R.K.,2012,Advances in Arachis genomics fo peanut improvement,Biotechnology Advances,30(3):639-651
Shirasawa K.,Bertioli D.J.,Varshney R.K.,Moretzsohn M.C.Leal-Bertioli S.C.M.,Thudi M.,Pandey M.K.,Rami J.F.Foncéka D.,Gowda M.V.C.,Qin H.D.,Guo B.Z.,Hong YB.,Liang X.Q.,Hirakawa H.,Tabata S.,and Isobe S.,2013Integrated consensus map of cultivated peanut and wild rela tives reveals structures of the A and B genomes of Arachi and divergence of the legume genomes,DNA Research dss042
Xu Y.B.,and Zhu L.H.,eds.,1994,Molecular quantitative genet ics,China Agriculture Press,Bejing,China,pp.110-179(徐云碧,朱立煌,1994,分子数量遗传学,中国农业出版社中国,北京,pp.110-179)
Xu Z.J.,Ren X.P.,Huang L.,Chen Y.N.,Zhou X.J.,Liao B.S.and Jiang H.F.,2015,Identification of SSR makers associat ed with bacterial wilt resistance of peanut through correla tion analysis,Zhongguo Youliao Zuowu Xuebao(Chines Journal of Oil Crop Sciences),37(6):803-810(徐志军,任小平,黄莉,陈玉宁,周小静,廖伯寿,姜慧芳,2015,花生青枯病抗性相关SSR标记的筛选鉴定,中国油料作物学报,37(6):803-810)
Yang X.L.,Zhou X.D.,Liu H.W.,Wang X.F.,Wu L.Q.,Li Z.K.Zhang Y.,Zhang G.Y.,and Ma Z.Y.,2013,AFLP marker associated with important agronomic traits in cotton,Mian hua Xuebao(Cotton Science),25(3):211-216(杨鑫雷,周晓栋,刘恒蔚,王省芬,吴立强,李志坤,张艳,张桂寅,马峙英,2013,AFLP标记与棉花重要农艺性状的关联研究棉花学报,25(3):211-216)
Zhao Y.L.,Zhang C.,Chen H,Yuan M.,Nipper R.,Prakash C.S.Zhuang W.J.,and He G.H.,2016,QTL mapping for bacteri al wilt resistance in peanut(Arachis hypogaea L.),Molecula Breeding,36(2):1-11
Zhong R.C.,Zhou M.L.,Zhao C.Z.,Hou L.,Li C.S.,Wang X.J.Tang R.H.,and Xia H.,2016,SSR marker developmen from peanut gynophore transcriptome sequencing,Plan Breeding,135(1):111-117
Zhou J.C.,Yang X.L.,Cui S.L.,Hou M.Y.,Chen H.Y.,Mu G.J.and Liu L.F.,2014,Correlation between SSR markers and gronomic traits in peanut(Arachis hypogaea L.),Zuowu Xuebao(Acta Agronomia Sinina),40(7):1197-1204(周金超,杨鑫雷,崔顺立,侯名语,陈焕英,穆国俊,刘立峰2014,花生SSR标记与农艺性状的相关性,作物学报,40(7):1197-1204)
Zhou X.J.,Xia Y.L.,Ren X.P.,Ren X.P.,Chen Y.L.,Huang L.Huang S.M.,Liao B.S.,Lei Y.,Yan L.Y.,and Jiang H.F.2014,Construction of a SNP-based genetic linkage map in cultivated peanut based on large scale marker developmen using next-generation double-digest restriction-site-associat ed DNA sequencing(dd RADseq),BMC Genomics,15:351
Cheng L.Q.,Tang M.,Ren X.P.,Huang L.,Chen W.G.,Li Z.D.Zhou X.J.,Chen Y.N.,Liao B.S.,and Jiang H.F.,2015Construction of genetic map and QTL analysis for mainstem height and total branch number in peanut(Arachis hypogae L.),Zuowu Xuebao(Acta Agronomca Sinica),41(6):979-987(成良强,唐梅,任小平,黄莉,陈伟刚,李振动,周小静,陈玉宁,廖伯寿,姜慧芳,2015,栽培种花生遗传图谱的构建及主茎高和总分枝数QTL分析,作物学报,41(6):979-987)
Chu Y.,Wu C.L.,Holbrook C.C.,Tillman B.L.,Person G.,and Ozias-Akins P.,2011,Marker-assisted selection to pyramid n ematode resistance and the high oleic trait in peanut,The Plant Genome,4(2):110-117
Cui S.L.,2010,Studies on the genetic diversity of peanu(Arachis hypogaea L.)landraces in Hebei Province,Thesi for M.S.,Agricultural University of Hebei,Supervisor:Liu L.F.,pp.10(崔顺立,2010,河北省花生(Arachis hypogae L.)地方品种遗传多样性研,硕士学位论文,河北农业大学,导师:刘立峰,pp.10)
Fang X.J.,Wu W.R.,and Tang J.L.,2002,DNA marker assisted in crop breeding,Science Press,Beijing,China,pp.41-45(方宣钧,吴为人,唐纪良,2002,作物DNA标记辅助育种,科学出版社,中国,北京,pp.41-45)
Gao J.R.,Han X.L.,Kong C.L.,Zhang Y.X.,Li A.F.,Ning T.Y.and Wang C.H.,2009,Applications of DA7200 near in frared apparatus in analyzing the quality of wheats,Shiyan shi Kexue(Laboratory Science),(1):173-176(高居荣,韩秀兰,孙彩玲,张永祥,李安飞,宁堂原,王承海,2009DA7200近红外仪在小麦品质分析中的应用研究,实验室科学,(1):173-176)
Guo J.B.,Huang L.,Chen L.Q.,Chen W.G.,Ren X.P.,Chen YN.,Zhou X.J.,Shen J.X.,and Jiang H.F.,2016,An integrat ed genetic linkage map from three F2populations of cultivat ed peanut(Arachis hypogaea L.),Zuowu Xuebao(Acta Agronomica Sinica),42(2):159-169(郭建斌,黄莉,成良强陈伟刚,任小平,陈玉宁,周小静,沈金雄,姜慧芳,2016利用3个F2群体整合高密度栽培种花生遗传连锁图,作物学报,42(2):159-169)
Huang L.,He H.Y.,Chen W.G.,Ren X.P.,Chen Y.N.,Zhou X.J.Xia Y.L.,Wang X.L.,Jiang X.G.,Liao B.S.,and Jiang H.F.2015,Quantitative trait locus analysis of agronomic and quality-related traits in cultivated peanut(Arachis hypogaea L.),Theor.Appl.Genet.,128(6):1103-1115
Khedikar Y.P.,Gowda M.V.C.,Sarvamangala C.,Patgar K.V.Upadhyaya H.D.,and Varshney R.K.,2010,A QTL study on late leaf spot and rust revealed one major QTL for molec ular breeding for rust resistance in groundnut(Arachis hypogaea L.),Theor.Appl.Genet.,121(5):971-984
Li X.P.,Xu Z.J.,Cai Y,Guo J.,B.,Huang L.,Ren X.P.,Li Z.,D.Chen W.G.,Luo H.Y.,Zhou X.J.,Chen Y.N.,Wu M.Y.and Jiang H.F.,2016,Quantitative trait locus analysis fo main quality traits in cultivated peanut(Arachis hypogae L.),Zhongguo Youliao Zuowu Xuebao(Chinese Journal o Oil Crop Sciences),38(4):415-422(李新平,徐志军,蔡岩郭建斌,黄莉,任小平,李振动,陈伟刚,罗怀勇,周小静陈玉宁,吴明煜,姜慧芳,2016,花生主要品质性状的QTL定位分析,中国油料作物学报,38(4):415-422)
Li Z.D.,Li X.P.,Huang L.,Ren X.P.,Chen Y.N.,Zhou X.J.,Liao B.S.,and Jiang H.F.,2015,Mapping of QTLs for pod size related traits in cultivated peanut(Arachis hypogaea L.)Zuowu Xuebao(Acta Agronomia Sinina),41(9):1313-1323(李振动,李新平,黄莉,任小平,陈玉宁,周小静,廖伯寿姜慧芳,2015,栽培种花生荚果大小相关性状QTL定位作物学报,41(9):1313-1323)
Liu L.F.,Dang P.M.,and Chen C.Y.,2015,Development and uti lization of In Del markers to identify peanut(Arachis hypo gaea)disease resistance,Frontiers in Plant Science,6:988
Pandey M.K.,Monyo E.,Ozias-Akins P.,Liang X.Q.,Guimare P.,Nigam S.M.,Upadhyaya H.D.,Janila P.,Zhang X.Y.Guo B.Z.,Cook D.R.,Bertioli D.J.,Michelmore R.,and Varshney R.K.,2012,Advances in Arachis genomics fo peanut improvement,Biotechnology Advances,30(3):639-651
Shirasawa K.,Bertioli D.J.,Varshney R.K.,Moretzsohn M.C.Leal-Bertioli S.C.M.,Thudi M.,Pandey M.K.,Rami J.F.Foncéka D.,Gowda M.V.C.,Qin H.D.,Guo B.Z.,Hong YB.,Liang X.Q.,Hirakawa H.,Tabata S.,and Isobe S.,2013Integrated consensus map of cultivated peanut and wild rela tives reveals structures of the A and B genomes of Arachi and divergence of the legume genomes,DNA Research dss042
Xu Y.B.,and Zhu L.H.,eds.,1994,Molecular quantitative genet ics,China Agriculture Press,Bejing,China,pp.110-179(徐云碧,朱立煌,1994,分子数量遗传学,中国农业出版社中国,北京,pp.110-179)
Xu Z.J.,Ren X.P.,Huang L.,Chen Y.N.,Zhou X.J.,Liao B.S.and Jiang H.F.,2015,Identification of SSR makers associat ed with bacterial wilt resistance of peanut through correla tion analysis,Zhongguo Youliao Zuowu Xuebao(Chines Journal of Oil Crop Sciences),37(6):803-810(徐志军,任小平,黄莉,陈玉宁,周小静,廖伯寿,姜慧芳,2015,花生青枯病抗性相关SSR标记的筛选鉴定,中国油料作物学报,37(6):803-810)
Yang X.L.,Zhou X.D.,Liu H.W.,Wang X.F.,Wu L.Q.,Li Z.K.Zhang Y.,Zhang G.Y.,and Ma Z.Y.,2013,AFLP marker associated with important agronomic traits in cotton,Mian hua Xuebao(Cotton Science),25(3):211-216(杨鑫雷,周晓栋,刘恒蔚,王省芬,吴立强,李志坤,张艳,张桂寅,马峙英,2013,AFLP标记与棉花重要农艺性状的关联研究棉花学报,25(3):211-216)
Zhao Y.L.,Zhang C.,Chen H,Yuan M.,Nipper R.,Prakash C.S.Zhuang W.J.,and He G.H.,2016,QTL mapping for bacteri al wilt resistance in peanut(Arachis hypogaea L.),Molecula Breeding,36(2):1-11
Zhong R.C.,Zhou M.L.,Zhao C.Z.,Hou L.,Li C.S.,Wang X.J.Tang R.H.,and Xia H.,2016,SSR marker developmen from peanut gynophore transcriptome sequencing,Plan Breeding,135(1):111-117
Zhou J.C.,Yang X.L.,Cui S.L.,Hou M.Y.,Chen H.Y.,Mu G.J.and Liu L.F.,2014,Correlation between SSR markers and gronomic traits in peanut(Arachis hypogaea L.),Zuowu Xuebao(Acta Agronomia Sinina),40(7):1197-1204(周金超,杨鑫雷,崔顺立,侯名语,陈焕英,穆国俊,刘立峰2014,花生SSR标记与农艺性状的相关性,作物学报,40(7):1197-1204)
Zhou X.J.,Xia Y.L.,Ren X.P.,Ren X.P.,Chen Y.L.,Huang L.Huang S.M.,Liao B.S.,Lei Y.,Yan L.Y.,and Jiang H.F.2014,Construction of a SNP-based genetic linkage map in cultivated peanut based on large scale marker developmen using next-generation double-digest restriction-site-associat ed DNA sequencing(dd RADseq),BMC Genomics,15:351