混合基因型紫皮高油酸花生新品系的筛选及分子鉴评
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摘要
采用YC4和YC9组合群体,该组合在F_4代通过分子测定基因型分别为Aabb、aaBb和AaBb杂合型。通过近红外检测技术检测,YC4组合中高油酸类型的表型频率为NIR值低于80%的占19.77%,高于80%的占80.23%。YC9组合中高油酸类型的表型频率为NIR值低于80%的占28.35%,高于80%的占71.65%。考种结果表明,筛选紫色种皮高油酸花生且产量高于‘冀花5号’花生品种50%以上的材料,YC4组合群体共筛选到材料69株,中果类型50株,大果类型19株。中果类型中荚果形状多为普通型,果嘴形状多为中等或明显;大果类型中荚果形状也多为普通型,果嘴形状多为中等或明显;YC9组合群体共筛选到19株材料,中果类型13株,大果类型6株。中果类型中荚果形状多为普通型,果嘴形状多为中等或明显;大果类型中荚果形状多为普通型或茧型,果嘴形状多为中等或非常明显。通过继代选择,获得3个紫色种皮高油酸新品系ZG-4-19-5-3、ZG-4-20-18-1和ZG-9-15-9-5,油酸NIR值分别为86.92%、85.77%和88.14%,分子鉴定结果与其表型一致。该批新种质材料的创制不仅提高了高油酸花生的医疗保健价值,同时对中国高油酸花生种质资源的丰富及品质育种具有重要意义。
YC4 and YC9 hybridization combinations, whose genotypes were Aabb, aaBb and AaBb identified by molecular detection in F_4, were adopted in this article. By using Near Infrared Spectrum, phenotype frequency of high-oleic acid typies in YC4 were detected. There were 19.77% individuals whose NIR value were less than 80%,and 80.23% were more than 80%. There are 28.35% individuals which NIR are less than 80% in YC9 combination and 71.65% are more than 80%. By seed investigation, the materials of high-oleic acid peanut with purple testa,of which the production was more than 50% compared with the peanut cultivar 'Jihua5', were selected in this research. In YC4 combination, a total of 69 materials were screened, with 50 plants belonging to middle pods and 19 plants to big pods. The pod traits of middle pods and big pods were mostly ordinary and the pods mouth traits were mostly medium or obvious. In YC9 combination, a total of 19 materials were screened, with 13 plants belonging to middle pods and 6 plants to big pods. The pod traits of middle pods were mostly ordinary and the pods mouth traits were mostly medium or obvious. While, the pod traits of big pods were mostly ordinary or cocoon shape and the pods mouth traits were mostly medium or very obvious. Through successive generations of selection, three purple testa with high-oleic acid lines ZG-4-19-5-3, ZG-4-20-18-1 and ZG-9-15-9-5 were obtained. The NIR values were 86.92%, 85.77%, and 88.14%, respectively, and the molecular identification results were consistent with their phenotypes. The creation of these new germplasm materials not only improves the health care value of high-oleic peanuts, but also has great significance for the enrichment of peanut germplasm resources and quality breeding in China.
YC4 and YC9 hybridization combinations, whose genotypes were Aabb, aaBb and AaBb identified by molecular detection in F_4, were adopted in this article. By using Near Infrared Spectrum, phenotype frequency of high-oleic acid typies in YC4 were detected. There were 19.77% individuals whose NIR value were less than 80%,and 80.23% were more than 80%. There are 28.35% individuals which NIR are less than 80% in YC9 combination and 71.65% are more than 80%. By seed investigation, the materials of high-oleic acid peanut with purple testa,of which the production was more than 50% compared with the peanut cultivar 'Jihua5', were selected in this research. In YC4 combination, a total of 69 materials were screened, with 50 plants belonging to middle pods and 19 plants to big pods. The pod traits of middle pods and big pods were mostly ordinary and the pods mouth traits were mostly medium or obvious. In YC9 combination, a total of 19 materials were screened, with 13 plants belonging to middle pods and 6 plants to big pods. The pod traits of middle pods were mostly ordinary and the pods mouth traits were mostly medium or obvious. While, the pod traits of big pods were mostly ordinary or cocoon shape and the pods mouth traits were mostly medium or very obvious. Through successive generations of selection, three purple testa with high-oleic acid lines ZG-4-19-5-3, ZG-4-20-18-1 and ZG-9-15-9-5 were obtained. The NIR values were 86.92%, 85.77%, and 88.14%, respectively, and the molecular identification results were consistent with their phenotypes. The creation of these new germplasm materials not only improves the health care value of high-oleic peanuts, but also has great significance for the enrichment of peanut germplasm resources and quality breeding in China.
引文
Blanco M.,and Villarroya J.,2002,NIR spectroscopy a rapid-response analytical tool,Trends Anal.Chem.,21(4):240-250
Chen J.,2011,Advances in genetics and breeding of high oleic acid peanut,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),12(2):190-196(陈静,2011,高油酸花生遗传育种研究进展,植物遗传资源学报,12(2):190-196)
Davis J.P.,Dean L.O.,Faircloth W.H.,and Sanders T.H.,2008,Physical and chemical characterizations of normal and high-oleic oils from nine commercial cultivars of peanut,J.Am.Oil Chem.Soc.,85(3):235-243
Han Z.Q.,Gao G.Q.,Zhou R.Y.,Tang R.H.,Zhong R.C.,Zhou C.Q.,and He L.Q.,2010,Inheritance of oleic,linoleic acid content and O/L ratio in high oleic acid Arachis hypogaea L.var.hirsuta,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),11(1):17-22(韩柱强,高国庆,周瑞阳,唐荣华,钟瑞春,周翠球,贺梁琼,2010,龙生型高油酸花生种质油酸亚油酸含量及其比值的遗传分析,植物遗传资源学报,11(1):17-22)
Hong Y.B.,Lin K.Y.,Zhou G.Y.,Li S.X.,Li Y.,and Liang X.Q.,2007,Genetic linkage analysis of SSR markers and the gene for dark purple testa color in peanut,Zhongguo Youliao Zuowu Xuebao(Chinese Journal of Oil Crop Sciences),29(1):35-38(洪彦彬,林坤耀,周桂元,李少雄,李艳,梁炫强,2007,花生深紫色种皮颜色基因的遗传分析及SSR标记,中国油料作物学报,29(1):35-38)
Hu X.H.,Shi Y.Q.,Miao H.R.,and Chen J.,2009,Genetic analysis of seed capsule color and correlative quality in peanut(Arachis hypogaea),Jiangxi Nongye Xuebao(Acta Agriculturae Jiangxi),21(3):27-28(胡晓辉,石运庆,苗华荣,陈静,2009,花生种皮颜色遗传及相关品质分析,江西农业学报,21(3):27-28)
Huang Z.R.,Sha S.,Rong Z.Q.,Liu H.Y.,Chen J.H.,and Zhu S.J.,2013,Rapid and nondestructive determination of fatty acid contents in shell-intact cottonseed by near infrared spectroscopy,Fenxi Huaxue(Chinese Journal of Analytical Chemistry),41(6):922-926(黄庄荣,沙莎,荣正勤,刘海英,陈进红,祝水金,2013,基于近红外技术快速无损分析整粒棉籽中的脂肪酸含量,分析化学,41(6):922-926)
Isleib T.G.,Wilson R.F.,and Novitzky W.P.,2006,Partial dominance,pleiotropism,and epistasis in the inheritance of the high-oleate trait in peanut,Crop Sci.,46(3):1331-1335
Li J.X.,Zhang H.L.,Yan Y.L.,Min S.G.,and Li Z.C.,2006,Establishment of math models of NIRS analysis for protein contents in seed and it's application in rice breeding,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),39(4):836-841(李君霞,张洪亮,严衍禄,闵顺耕,李自超,2006,水稻蛋白质近红外定量模型的创建及在育种中的应用,中国农业科学,39(4):836-841)
Li L.,He M.J.,Cui S.L.,Hou M.Y.,Chen H.Y.,Yang X.L.,Wang P.C.,Liu L.F.,and Mu G.J.,2014,The development and identification of new peanut germplasm materials with high oleic acid and medium pod,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),47(19):3898-3906(李丽,何美静,崔顺立,侯名语,陈焕英,杨鑫雷,王鹏超,刘立峰,穆国俊,2014,高油酸、中果型花生新材料的创制与鉴定,中国农业科学,47(19):3898-3906)
Liang N.N.,Zhang L.X.,Wang X.L.,Tan B.B.,and Liang Y.Z.,2011,Identification of fatty acids in vegetable oils by mass spectrometry and equivalent chain length,Fenxi Huaxue(Chinese Journal of Analytical Chemistry),39(8):1166-1170(梁楠楠,张良晓,王向利,谭斌斌,梁逸曾,2011,质谱特征结合等效链长定性分析植物油中的脂肪酸,分析化学,39(8):1166-1170)
Miao H.R.,Cui F.G.,Hu X.H.,Shi Y.Q.,and Chen J.,2015,Breeding and characteristics of high oleic peanut(Arachis hypogaea L.)new varieties,Huasheng Xuebao(Journal of Peanut Science),44(1):64-65(苗华荣,崔凤高,胡晓辉,石运庆,陈静,2015,高油酸系列花生新品种的选育及特征,花生学报,44(1):64-65)
Stalker T.,and Wilson R.F.,2016,Peanuts:genetics,processing,and utilization,Academic Press and AOCS Press,Urbana,USA,pp.478
Toborek M.,Lee Y.W.,Garrido R.,Kaiser S.,and Hennig B.,2002,Unsaturated fatty acids selectively induce an inflammatory environment in human endothelial cells,Am.J.Clin.Nutr.,75(1):119-125
Wang C.T.,Tang Y.Y.,Wang X.Z.,Wu Q.,Sun Q.X.,Zhu L.G.,and Wang Z.W.,2016,Appraisal of productivity and fatty acid profiles of seven new high-oleic peanut cultivars,Shandong Nongye Kexue(Shandong Agricultural Sciences),48(5):31-34(王传堂,唐月异,王秀贞,吴琪,孙全喜,朱立贵,王志伟,2016,7个高油酸花生新品种的丰产性和脂肪酸成分评价,山东农业科学,48(5):31-34)
Wang C.T.,Wang X.Z.,Tang Y.Y.,Wu Q.,Sun Q.X.,Zhang J.C.,Cui F.G.,Li L.M.,and Miao H.C.,2015,High-oleic peanut germplasm enhancement and cultivar releases in China:main achievements and suggestions,Huasheng Xuebao(Journal of Peanut Science),44(2):49-53(王传堂,王秀贞,唐月异,吴琪,孙全喜,张建成,崔凤高,李利民,苗昊翠,2015,中国高油酸花生种质创制、品种选育进展与建议,花生学报,44(2):49-53)
Wei L.M.,Yan Y.L.,and Dai J.R.,2004,Determining protein and starch contents of whole maize kernel by near infrared re flectance spectroscopy(NIRS),Zhongguo Nongye Kexue(Scientia Agricultura Sinica),37(5):630-633(魏良明,严衍禄,戴景瑞,2004,近红外反射光谱测定玉米完整籽粒蛋白质和淀粉含量的研究,中国农业科学,37(5):630-633)
Wu L.R.,Li Z.C.,and Qiu Q.S.,2002,F1generation heterosis of peanut varieties with special testa color,Henongxue Bao(Journal of Nuclear Agricultural Sciences),16(4):203-207(吴兰荣,李正超,邱庆树,2002,特殊种皮颜色花生杂种优势的研究,核农学报,16(4):203-207)
Yan D.,Zhao C.B.,Xie D.S.,Hu M.B.,Li L.,and Wu C.J.,2015,The exploitation and utilization of peanut coat,Yatai Chuantong Yiyao(Asia-Pacific Traditional Medicine),11(20):51-54(严丹,赵重博,解达帅,胡美变,黎量,吴纯洁,2015,花生衣开发利用研究进展,亚太传统医药,11(20):51-54)
Yan L.,Jiang C.Z.,Yu X.H.,Yan C.Y.,and Zhang M.C.,2008,Development and reliability of near infrared spectroscopy(NIS)models of protein and oil content in soybean,Dadou Kexue(Soybean Science),27(5):833-837(闫龙,蒋春志,于向鸿,杨春燕,张孟臣,2008,大豆粗蛋白、粗脂肪含量近红外检测模型建立及可靠性分析,大豆科学,27(5):833-837)
Yao X.M.,Zhang R.Y.,Li X.H.,Cheng A.H.,Zhang X.B.,and Gao C.X.,2006,Study on analysis of soybean quality by near infrared transmittance spectroscopy,Dadou Kexue(Soybean Science),5(4):417-420(姚鑫淼,张瑞英,李霞辉,程爱华,张晓波,高春霞,2006,近红外透射光谱法(NITS)分析大豆品质的研究,大豆科学,25(4):417-420)
Yu S.L.,ZhuY.J.,Min P.,Liu H.,and Cao Y.L.,2003,Nondestructive determination of protein and oil content in peanut seeds by near infrared diffuse reflectance spectroscopy,Huasheng Xuebao(Journal of Peanut Science)32(S):138-143(禹山林,朱雨杰,闵平,刘华,曹玉良,2003,傅立叶近红外漫反射非破坏性测定花生种蛋白质及含油量,花生学报,32(S):138-143)
Zhou H.,Yan C.X.,Guo L.C.,Liu Y.,and Shan S.H.,2012,Rapid extraction of genomic DNA from peanut by CTABmethod,Shandong Nongye Kexue(Shandong Agricultural Sciences),44(7):8-9(周浩,闫彩霞,郭凌超,刘宇,单世华,2012,CTAB法少量快速提取花生基因组DNA,山东农业科学,44(7):8-9)
Chen J.,2011,Advances in genetics and breeding of high oleic acid peanut,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),12(2):190-196(陈静,2011,高油酸花生遗传育种研究进展,植物遗传资源学报,12(2):190-196)
Davis J.P.,Dean L.O.,Faircloth W.H.,and Sanders T.H.,2008,Physical and chemical characterizations of normal and high-oleic oils from nine commercial cultivars of peanut,J.Am.Oil Chem.Soc.,85(3):235-243
Han Z.Q.,Gao G.Q.,Zhou R.Y.,Tang R.H.,Zhong R.C.,Zhou C.Q.,and He L.Q.,2010,Inheritance of oleic,linoleic acid content and O/L ratio in high oleic acid Arachis hypogaea L.var.hirsuta,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),11(1):17-22(韩柱强,高国庆,周瑞阳,唐荣华,钟瑞春,周翠球,贺梁琼,2010,龙生型高油酸花生种质油酸亚油酸含量及其比值的遗传分析,植物遗传资源学报,11(1):17-22)
Hong Y.B.,Lin K.Y.,Zhou G.Y.,Li S.X.,Li Y.,and Liang X.Q.,2007,Genetic linkage analysis of SSR markers and the gene for dark purple testa color in peanut,Zhongguo Youliao Zuowu Xuebao(Chinese Journal of Oil Crop Sciences),29(1):35-38(洪彦彬,林坤耀,周桂元,李少雄,李艳,梁炫强,2007,花生深紫色种皮颜色基因的遗传分析及SSR标记,中国油料作物学报,29(1):35-38)
Hu X.H.,Shi Y.Q.,Miao H.R.,and Chen J.,2009,Genetic analysis of seed capsule color and correlative quality in peanut(Arachis hypogaea),Jiangxi Nongye Xuebao(Acta Agriculturae Jiangxi),21(3):27-28(胡晓辉,石运庆,苗华荣,陈静,2009,花生种皮颜色遗传及相关品质分析,江西农业学报,21(3):27-28)
Huang Z.R.,Sha S.,Rong Z.Q.,Liu H.Y.,Chen J.H.,and Zhu S.J.,2013,Rapid and nondestructive determination of fatty acid contents in shell-intact cottonseed by near infrared spectroscopy,Fenxi Huaxue(Chinese Journal of Analytical Chemistry),41(6):922-926(黄庄荣,沙莎,荣正勤,刘海英,陈进红,祝水金,2013,基于近红外技术快速无损分析整粒棉籽中的脂肪酸含量,分析化学,41(6):922-926)
Isleib T.G.,Wilson R.F.,and Novitzky W.P.,2006,Partial dominance,pleiotropism,and epistasis in the inheritance of the high-oleate trait in peanut,Crop Sci.,46(3):1331-1335
Li J.X.,Zhang H.L.,Yan Y.L.,Min S.G.,and Li Z.C.,2006,Establishment of math models of NIRS analysis for protein contents in seed and it's application in rice breeding,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),39(4):836-841(李君霞,张洪亮,严衍禄,闵顺耕,李自超,2006,水稻蛋白质近红外定量模型的创建及在育种中的应用,中国农业科学,39(4):836-841)
Li L.,He M.J.,Cui S.L.,Hou M.Y.,Chen H.Y.,Yang X.L.,Wang P.C.,Liu L.F.,and Mu G.J.,2014,The development and identification of new peanut germplasm materials with high oleic acid and medium pod,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),47(19):3898-3906(李丽,何美静,崔顺立,侯名语,陈焕英,杨鑫雷,王鹏超,刘立峰,穆国俊,2014,高油酸、中果型花生新材料的创制与鉴定,中国农业科学,47(19):3898-3906)
Liang N.N.,Zhang L.X.,Wang X.L.,Tan B.B.,and Liang Y.Z.,2011,Identification of fatty acids in vegetable oils by mass spectrometry and equivalent chain length,Fenxi Huaxue(Chinese Journal of Analytical Chemistry),39(8):1166-1170(梁楠楠,张良晓,王向利,谭斌斌,梁逸曾,2011,质谱特征结合等效链长定性分析植物油中的脂肪酸,分析化学,39(8):1166-1170)
Miao H.R.,Cui F.G.,Hu X.H.,Shi Y.Q.,and Chen J.,2015,Breeding and characteristics of high oleic peanut(Arachis hypogaea L.)new varieties,Huasheng Xuebao(Journal of Peanut Science),44(1):64-65(苗华荣,崔凤高,胡晓辉,石运庆,陈静,2015,高油酸系列花生新品种的选育及特征,花生学报,44(1):64-65)
Stalker T.,and Wilson R.F.,2016,Peanuts:genetics,processing,and utilization,Academic Press and AOCS Press,Urbana,USA,pp.478
Toborek M.,Lee Y.W.,Garrido R.,Kaiser S.,and Hennig B.,2002,Unsaturated fatty acids selectively induce an inflammatory environment in human endothelial cells,Am.J.Clin.Nutr.,75(1):119-125
Wang C.T.,Tang Y.Y.,Wang X.Z.,Wu Q.,Sun Q.X.,Zhu L.G.,and Wang Z.W.,2016,Appraisal of productivity and fatty acid profiles of seven new high-oleic peanut cultivars,Shandong Nongye Kexue(Shandong Agricultural Sciences),48(5):31-34(王传堂,唐月异,王秀贞,吴琪,孙全喜,朱立贵,王志伟,2016,7个高油酸花生新品种的丰产性和脂肪酸成分评价,山东农业科学,48(5):31-34)
Wang C.T.,Wang X.Z.,Tang Y.Y.,Wu Q.,Sun Q.X.,Zhang J.C.,Cui F.G.,Li L.M.,and Miao H.C.,2015,High-oleic peanut germplasm enhancement and cultivar releases in China:main achievements and suggestions,Huasheng Xuebao(Journal of Peanut Science),44(2):49-53(王传堂,王秀贞,唐月异,吴琪,孙全喜,张建成,崔凤高,李利民,苗昊翠,2015,中国高油酸花生种质创制、品种选育进展与建议,花生学报,44(2):49-53)
Wei L.M.,Yan Y.L.,and Dai J.R.,2004,Determining protein and starch contents of whole maize kernel by near infrared re flectance spectroscopy(NIRS),Zhongguo Nongye Kexue(Scientia Agricultura Sinica),37(5):630-633(魏良明,严衍禄,戴景瑞,2004,近红外反射光谱测定玉米完整籽粒蛋白质和淀粉含量的研究,中国农业科学,37(5):630-633)
Wu L.R.,Li Z.C.,and Qiu Q.S.,2002,F1generation heterosis of peanut varieties with special testa color,Henongxue Bao(Journal of Nuclear Agricultural Sciences),16(4):203-207(吴兰荣,李正超,邱庆树,2002,特殊种皮颜色花生杂种优势的研究,核农学报,16(4):203-207)
Yan D.,Zhao C.B.,Xie D.S.,Hu M.B.,Li L.,and Wu C.J.,2015,The exploitation and utilization of peanut coat,Yatai Chuantong Yiyao(Asia-Pacific Traditional Medicine),11(20):51-54(严丹,赵重博,解达帅,胡美变,黎量,吴纯洁,2015,花生衣开发利用研究进展,亚太传统医药,11(20):51-54)
Yan L.,Jiang C.Z.,Yu X.H.,Yan C.Y.,and Zhang M.C.,2008,Development and reliability of near infrared spectroscopy(NIS)models of protein and oil content in soybean,Dadou Kexue(Soybean Science),27(5):833-837(闫龙,蒋春志,于向鸿,杨春燕,张孟臣,2008,大豆粗蛋白、粗脂肪含量近红外检测模型建立及可靠性分析,大豆科学,27(5):833-837)
Yao X.M.,Zhang R.Y.,Li X.H.,Cheng A.H.,Zhang X.B.,and Gao C.X.,2006,Study on analysis of soybean quality by near infrared transmittance spectroscopy,Dadou Kexue(Soybean Science),5(4):417-420(姚鑫淼,张瑞英,李霞辉,程爱华,张晓波,高春霞,2006,近红外透射光谱法(NITS)分析大豆品质的研究,大豆科学,25(4):417-420)
Yu S.L.,ZhuY.J.,Min P.,Liu H.,and Cao Y.L.,2003,Nondestructive determination of protein and oil content in peanut seeds by near infrared diffuse reflectance spectroscopy,Huasheng Xuebao(Journal of Peanut Science)32(S):138-143(禹山林,朱雨杰,闵平,刘华,曹玉良,2003,傅立叶近红外漫反射非破坏性测定花生种蛋白质及含油量,花生学报,32(S):138-143)
Zhou H.,Yan C.X.,Guo L.C.,Liu Y.,and Shan S.H.,2012,Rapid extraction of genomic DNA from peanut by CTABmethod,Shandong Nongye Kexue(Shandong Agricultural Sciences),44(7):8-9(周浩,闫彩霞,郭凌超,刘宇,单世华,2012,CTAB法少量快速提取花生基因组DNA,山东农业科学,44(7):8-9)