基于综合生物标志物响应评价苯并芘对脊尾白虾(Exopalaemon carinicauda)的毒性效应
|
摘要
苯并[a]芘(Ba P)是一类典型多环芳烃(PAHs)污染物。为了定量评价Ba P对脊尾白虾(Exopalaemon carinicauda)的毒性效应,采用水体暴露实验,研究了脊尾白虾肝胰脏、肌肉组织的5种生物标志物(EROD、GST、SOD、CAT、MDA)对Ba P暴露的响应,并应用综合生物标志物响应(IBR)定量评价了Ba P对肝胰脏、肌肉组织的毒性差异。结果显示:肝胰脏、肌肉组织的EROD、GST、SOD、CAT活性整体呈现诱导-抑制的规律性变化,除了0.05μg/L实验组CAT外,其余各实验组肝胰脏组织生物标志物活性在实验结束时均显著高于对照组(P<0.05),除了0.45μg/L实验组EROD、CAT外,其余各浓度组肌肉组织生物标志物活性在实验结束时均与对照组无显著性差异(P>0.05),MDA含量呈现持续上升的趋势,在实验结束时各实验组均显著高于对照组(P<0.05);IBR值存在时间效应和剂量效应。研究结果表明,肝胰脏、肌肉组织对Ba P的暴露响应存在差异性,肝胰脏生物标志物综合应激活性高于肌肉组织。
Benzo[a]pyrene(Ba P) is a representative polycyclic aromatic hydrocarbon(PAHs), which is ubiquitous in the environment. To determine the toxic effects induced by Ba P, biomarkers(EROD, GST, SOD, CAT, MDA) responses in muscle and hepatopancreas of Exopalaemon carinicauda exposed to Ba P were studied, and the toxicity of Ba P assessed by integrated biomarker response(IBR). The results show that these biomarkers showed certain induction-inhibition. The activities in all exposure concentrations in hepatopancreas were higher than that of the control(P<0.05) except for CAT in 0.05μg/L, and could resume to a normal level(P>0.05) except for EROD and CAT in 0.45μg/L. Meanwhile, the content of MDA in hepatopancreas presented a rising trend and higher than the control(P<0.05). The IBR(integrated biomarker response) was proved as a useful index to the quantification of various biomarker responses to toxic chemical exposures, and showed that the sensitivity of hepatopancreas was higher than that of the muscle.
Benzo[a]pyrene(Ba P) is a representative polycyclic aromatic hydrocarbon(PAHs), which is ubiquitous in the environment. To determine the toxic effects induced by Ba P, biomarkers(EROD, GST, SOD, CAT, MDA) responses in muscle and hepatopancreas of Exopalaemon carinicauda exposed to Ba P were studied, and the toxicity of Ba P assessed by integrated biomarker response(IBR). The results show that these biomarkers showed certain induction-inhibition. The activities in all exposure concentrations in hepatopancreas were higher than that of the control(P<0.05) except for CAT in 0.05μg/L, and could resume to a normal level(P>0.05) except for EROD and CAT in 0.45μg/L. Meanwhile, the content of MDA in hepatopancreas presented a rising trend and higher than the control(P<0.05). The IBR(integrated biomarker response) was proved as a useful index to the quantification of various biomarker responses to toxic chemical exposures, and showed that the sensitivity of hepatopancreas was higher than that of the muscle.
引文
任加云,潘鲁青,苗晶晶,2006.苯并(a)芘和苯并(k)荧蒽混合物对栉孔扇贝毒理学指标的影响.环境科学学报,26(7):1180-1186
李康,周忠良,陈立侨等,2006.苯并[a]芘对鲫鱼生物标志物的影响研究.环境科学研究,19(1):91-95
蒋玫,李磊,沈新强等,2015.基于整合生物标志物响应指数评价0号柴油对黑鲷(Sparus macrocephalus)的毒性效应.生态与农村环境学报,31(2):230-237
Banni M,Bouraoui Z,Ghedira J et al,2009.Acute effects of benzo[a]pyrene on liver phase I and II enzymes,and DNAdamage on sea bream Sparus aurata.Fish Physiology and Biochemistry,35(2):293-299
Da Silva Rocha A J,Gomes V,De Arruda Campos Rocha Passos M J et al,2012.EROD activity and genotoxicity in the seabob shrimp Xiphopenaeus kroyeri exposed to benzo[a]pyrene(Ba P)concentrations.Environmental Toxicology and Pharmacology,34(3):995-1003
Gadagbui B K M,James M O,2000.Activities of affinity-isolated glutathione S-transferase(GST)from channel catfish whole intestine.Aquatic Toxicology,49(1-2):27-37
Gagnon M M,Holdway D A,2000.EROD induction and biliary metabolite excretion following exposure to the water accommodated fraction of crude oil and to chemically dispersed crude oil.Archives of Environmental Contamination and Toxicology,38(1):70-77
Haasch M L,Lech J J,Prince R et al,1993.Caged and wild fish:Induction of hepatic cytochrome P-450(CYP1A1)as an environmental biomonitor.Environmental Toxicology and Chemistry,12(5):885-895
Habig W H,Pabst M J,Jakoby W B,1974.Glutathione S-transferases.The first enzymatic step in mercapturic acid formation.Journal of Biological Chemistry,249(22):7130-7139
Hou J,You G X,Xu Y et al,2016.Antioxidant enzyme activities as biomarkers of fluvial biofilm to Zn O NPs ecotoxicity and the Integrated Biomarker Responses(IBR)assessment.Ecotoxicology and Environmental Safety,133:10-17
Kim J H,Kim W K,2016.Use of the integrated biomarker response to measure the effect of short-term exposure to dibenz[a,h]anthracene in common carp(Cyprinus carpio).Bulletin of Environmental Contamination and Toxicology,96(4):496-501
Lam P K S,Gray J S,2003.The use of biomarkers in environmental monitoring programmes.Marine Pollution Bulletin,46(2):182-186
Pohl R J,Fouts J R,1980.A rapid method for assaying the metabolism of 7-ethoxyresorufin by microsomal subcellular fractions.Analytical Biochemistry,107(1):150-155
Ren X Y,Pan L Q,Lin W,2015.Toxic effects upon exposure to benzo[a]pyrene in juvenile white shrimp Litopenaeus vannamei.Environmental Toxicology and Pharmacology,39(1):194-207
Santos M A,Pacheco M,Ahmad I,2004.Anguilla anguilla L.antioxidants responses to in situ bleached kraft pulp mill effluent outlet exposure.Environment International,30(3):301-308
SoléM,Porte C,Albaigés J,1995.The use of biomarkers for assessing the effects of organic pollution in mussels.Science of the Total Environment,159(2-3):147-153
SoléM,2000.Assessment of the results of chemical analyses combined with the biological effects of organic pollution on mussels.Trac Trends in Analytical Chemistry,19(1):1-9
Thomas J P,Maiorino M,Ursini F et al,1990.Protective action of phospholipid hydroperoxide glutathione peroxidase against membrane-damaging lipid peroxidation.In situ reduction of phospholipid and cholesterol hydroperoxides.Journal of Biological Chemistry,265(1):454-461
Wang S H,Wang X H,Hong H S,2005.Specific characteristic of tissue bioaccumulation of PAHs in green lipped mussels(Perna viridis)at different growth stages.Marine Environmental Science,24(3):29-32
李康,周忠良,陈立侨等,2006.苯并[a]芘对鲫鱼生物标志物的影响研究.环境科学研究,19(1):91-95
蒋玫,李磊,沈新强等,2015.基于整合生物标志物响应指数评价0号柴油对黑鲷(Sparus macrocephalus)的毒性效应.生态与农村环境学报,31(2):230-237
Banni M,Bouraoui Z,Ghedira J et al,2009.Acute effects of benzo[a]pyrene on liver phase I and II enzymes,and DNAdamage on sea bream Sparus aurata.Fish Physiology and Biochemistry,35(2):293-299
Da Silva Rocha A J,Gomes V,De Arruda Campos Rocha Passos M J et al,2012.EROD activity and genotoxicity in the seabob shrimp Xiphopenaeus kroyeri exposed to benzo[a]pyrene(Ba P)concentrations.Environmental Toxicology and Pharmacology,34(3):995-1003
Gadagbui B K M,James M O,2000.Activities of affinity-isolated glutathione S-transferase(GST)from channel catfish whole intestine.Aquatic Toxicology,49(1-2):27-37
Gagnon M M,Holdway D A,2000.EROD induction and biliary metabolite excretion following exposure to the water accommodated fraction of crude oil and to chemically dispersed crude oil.Archives of Environmental Contamination and Toxicology,38(1):70-77
Haasch M L,Lech J J,Prince R et al,1993.Caged and wild fish:Induction of hepatic cytochrome P-450(CYP1A1)as an environmental biomonitor.Environmental Toxicology and Chemistry,12(5):885-895
Habig W H,Pabst M J,Jakoby W B,1974.Glutathione S-transferases.The first enzymatic step in mercapturic acid formation.Journal of Biological Chemistry,249(22):7130-7139
Hou J,You G X,Xu Y et al,2016.Antioxidant enzyme activities as biomarkers of fluvial biofilm to Zn O NPs ecotoxicity and the Integrated Biomarker Responses(IBR)assessment.Ecotoxicology and Environmental Safety,133:10-17
Kim J H,Kim W K,2016.Use of the integrated biomarker response to measure the effect of short-term exposure to dibenz[a,h]anthracene in common carp(Cyprinus carpio).Bulletin of Environmental Contamination and Toxicology,96(4):496-501
Lam P K S,Gray J S,2003.The use of biomarkers in environmental monitoring programmes.Marine Pollution Bulletin,46(2):182-186
Pohl R J,Fouts J R,1980.A rapid method for assaying the metabolism of 7-ethoxyresorufin by microsomal subcellular fractions.Analytical Biochemistry,107(1):150-155
Ren X Y,Pan L Q,Lin W,2015.Toxic effects upon exposure to benzo[a]pyrene in juvenile white shrimp Litopenaeus vannamei.Environmental Toxicology and Pharmacology,39(1):194-207
Santos M A,Pacheco M,Ahmad I,2004.Anguilla anguilla L.antioxidants responses to in situ bleached kraft pulp mill effluent outlet exposure.Environment International,30(3):301-308
SoléM,Porte C,Albaigés J,1995.The use of biomarkers for assessing the effects of organic pollution in mussels.Science of the Total Environment,159(2-3):147-153
SoléM,2000.Assessment of the results of chemical analyses combined with the biological effects of organic pollution on mussels.Trac Trends in Analytical Chemistry,19(1):1-9
Thomas J P,Maiorino M,Ursini F et al,1990.Protective action of phospholipid hydroperoxide glutathione peroxidase against membrane-damaging lipid peroxidation.In situ reduction of phospholipid and cholesterol hydroperoxides.Journal of Biological Chemistry,265(1):454-461
Wang S H,Wang X H,Hong H S,2005.Specific characteristic of tissue bioaccumulation of PAHs in green lipped mussels(Perna viridis)at different growth stages.Marine Environmental Science,24(3):29-32