壳聚糖山苓祛斑凝胶的高效液相指纹图谱
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摘要
目的建立壳聚糖山苓祛斑凝胶的高效液相(HPLC)指纹图谱分析方法。方法采用Agilent C18色谱柱(4.6 mm×200 mm,5μm),流动相为甲醇-0.1%磷酸,梯度洗脱,检测波长286 nm,流速1.0 m L·min~(-1),柱温30℃,采用国家药典委员会"中药色谱指纹图谱相似度评价系统"(2010年版)软件处理图谱。结果建立了壳聚糖山苓祛斑凝胶的HPLC指纹图谱,以阿魏酸为参照物,标出10个共有峰,10批壳聚糖山苓祛斑凝胶指纹图谱相似度均>0.9。结论该实验所建立的HPLC指纹图谱专属性强,且操作方法简便可靠,可用于壳聚糖山苓祛斑凝胶的质量评价。
Objective To establish HPLC fingerprint and control the quality of chitosan Shanling Quban gels.Methods HPLC was applied to analyze the constituents of chitosan Shanling Quban gels. Using Agilent C18 Columns( 4.6 mm×200 mm,5 μm). According to the National Pharmacopoeia Commission " HPLC Chromatographic Fingerprint Similarity Evaluation System"( 2010 Edition),the chromatography was performed with methanol-0.1% phosphoric acid as mobile phase in gradient elution. The detection wavelength was 286 nm and the flow rate and column temperature was seted at 1.0 m L·min~(-1) and30 ℃,respectively. Results The HPLC fingerprints of chitosan Shanling Quban gels were established,and 10 common characteristic peaks were labeled. The similarity of HPLC fingerprint was 0. 9 for 10 batches of samples. Conclusion The HPLC fingerprint profile established in this experiment is highly specific and the method is simple and reliable which could reflect the quality of chitosan Shanling Quban gels.
Objective To establish HPLC fingerprint and control the quality of chitosan Shanling Quban gels.Methods HPLC was applied to analyze the constituents of chitosan Shanling Quban gels. Using Agilent C18 Columns( 4.6 mm×200 mm,5 μm). According to the National Pharmacopoeia Commission " HPLC Chromatographic Fingerprint Similarity Evaluation System"( 2010 Edition),the chromatography was performed with methanol-0.1% phosphoric acid as mobile phase in gradient elution. The detection wavelength was 286 nm and the flow rate and column temperature was seted at 1.0 m L·min~(-1) and30 ℃,respectively. Results The HPLC fingerprints of chitosan Shanling Quban gels were established,and 10 common characteristic peaks were labeled. The similarity of HPLC fingerprint was 0. 9 for 10 batches of samples. Conclusion The HPLC fingerprint profile established in this experiment is highly specific and the method is simple and reliable which could reflect the quality of chitosan Shanling Quban gels.
引文
[1]刘东方,赵丽娜,李银峰,等.中药指纹图谱技术的研究进展及应用[J].中草药,2016,47(26):4085-4094.
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[11]项妤,张贵君,甘德全.HPLC测定CO2超临界萃取的当归油药效组分[J].中国实验方剂学杂志,2012,18(8):64-67.
[12]曾彬,刘玉红,刘云华,等.HPLC指纹图谱对比研究川芎和当归的化学成分[J].华西药学杂志,2012,27(5):578-581.
[13]曹伟宇,贺文娟,雷丸,等.HPLC法同时测定干髓糊剂中丁香酚和麝香草酚的含量[J].中国药房,2017,28(30):4268-4271.
[14]张锐,卢金清,江汉美,等.高效液相色谱法测定不同产地丁香中丁香酚含量研究[J].亚太传统医药,2015,11(12):36-38.
[15]陈军,舒祥,余南才.山苓祛斑凝胶剂提取物质量标准研究[J].中国民族民间医药,2016,25(21):11-13.
[16]郭红叶,匡颖,闫小平,等.祛斑凝胶剂质量标准[J].中国实验方剂学杂志,2014,20(14):45-47.
[17]黄燕,戴余雯,焦海胜,等.神经性皮炎凝胶剂的质量标准研究[J].时珍国医国药,2016,27(11):2646-2649.
[18]张立军,李芸,戴海蓉,等.高乌头炮制品的HPLC指纹图谱研究及其2种生物碱成分含量测定[J].中国现代应用药学,2018,35(1):57-62.
[19]王露晨,张欢欢,余陈欢,等.石荠苧黄酮提取物的指纹图谱与含量测定分析[J].中国现代应用药学,2018,35(2):199-203.
[2]高华荣,孙相民.中药指纹图谱在现代中药制剂中的应用[J].中国实用医药,2009,4(14):240-241.
[3]孙国祥,闫波,侯志飞,等.中药色谱指纹图谱评价方法研究进展[J].中南药学,2015,13(7):673-681.
[4]黄群莲,罗颖,李芹,等.黄柏颗粒高效液相色谱指纹图谱[J].医药导报,2016,35(10):1113-1116.
[5]刘文琴,王燕霞,陈芳,等.参灵草口服液HPLC指纹图谱及质量评价[J].中国实验方剂学杂志,2016,22(23):83-88.
[6]李兵,刘雪梅,陈建惠,等.过塘蛇颗粒剂高效液相色谱指纹图谱的研究[J].时珍国医国药,2016,27(10):2382-2383.
[7]程巧鸳,张文婷,陈碧莲,等.普乐安片HPLC指纹图谱研究[J].中成药,2016,38(1):94-97.
[8]江洁怡,胥爱丽,李养学,等.白芷配方颗粒的指纹图谱研究[J].今日药学,2017,27(9):612-615.
[9]卢晓琳,马逾英,郑光雅,等.白芷提取物的HPLC指纹图谱研究[J].华西药学杂志,2013,28(4):398-400.
[10]杨先国,彭学著,彭攸灵,等.茶陵白芷的高效液相色谱指纹图谱及模式识别研究[J].中南药学,2016,14(9):924-927.
[11]项妤,张贵君,甘德全.HPLC测定CO2超临界萃取的当归油药效组分[J].中国实验方剂学杂志,2012,18(8):64-67.
[12]曾彬,刘玉红,刘云华,等.HPLC指纹图谱对比研究川芎和当归的化学成分[J].华西药学杂志,2012,27(5):578-581.
[13]曹伟宇,贺文娟,雷丸,等.HPLC法同时测定干髓糊剂中丁香酚和麝香草酚的含量[J].中国药房,2017,28(30):4268-4271.
[14]张锐,卢金清,江汉美,等.高效液相色谱法测定不同产地丁香中丁香酚含量研究[J].亚太传统医药,2015,11(12):36-38.
[15]陈军,舒祥,余南才.山苓祛斑凝胶剂提取物质量标准研究[J].中国民族民间医药,2016,25(21):11-13.
[16]郭红叶,匡颖,闫小平,等.祛斑凝胶剂质量标准[J].中国实验方剂学杂志,2014,20(14):45-47.
[17]黄燕,戴余雯,焦海胜,等.神经性皮炎凝胶剂的质量标准研究[J].时珍国医国药,2016,27(11):2646-2649.
[18]张立军,李芸,戴海蓉,等.高乌头炮制品的HPLC指纹图谱研究及其2种生物碱成分含量测定[J].中国现代应用药学,2018,35(1):57-62.
[19]王露晨,张欢欢,余陈欢,等.石荠苧黄酮提取物的指纹图谱与含量测定分析[J].中国现代应用药学,2018,35(2):199-203.