主成分分析结合均匀设计法用于优选桂芍巴布贴制备工艺的研究
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摘要
目的运用主成分分析建立一种中药多指标的综合评价方法,并将其应用于桂芍巴布贴制备工艺的优选研究。方法以巴布剂的外观性状、初黏力、持黏力、剥离强度为指标,应用主成分分析求得总因子得分(F),以F为综合指标,采用均匀设计法对聚丙烯酸钠NP-700、甘羟铝、甘油等的用量进行优化,确定最佳处方比例。结果桂芍巴布贴的最佳处方为NP-700-PVP K-30-甘羟铝-EDTA-2Na-酒石酸-甘油-浸膏(8∶1∶0.25∶0.07∶0.15∶25∶20),酒石酸、PVP K-30、NP-700、甘羟铝、甘油、EDTA-2Na是最主要的影响因素。结论优选的桂芍巴布贴易涂布、膏体均匀、黏性较好,可为桂芍巴布贴的新剂型开发提供基础;同时主成分分析法为中药多指标评价研究提供借鉴。
Objective To establish a comprehensive evaluation method based on principal component analysis(PCA) for the study of multiple indexes of Chinese medicine and to choose the best preparation technology of Gui-Shao Cataplasm with this method. Methods The appearance description, initial bonding strength, endurance bonding strength, and peel strength were taken as indexes, the total factor scores were calculated using PCA and were employed to evaluate the quality of Gui-Shao Cataplasm, the uniform design test was used to optimize the amounts of sodium polyacrylate NP-700, aluminium glycinate, glycerin, etc. for Gui-Shao Cataplasm. Results The optimal ratio of the prescription was as following: sodium polyacrylate NP-700-PVP K-30-aluminium glycinate-EDTA- 2Na-tartaric acid-glycerin-extract(8∶1∶0.25∶0.07∶0.15∶25∶20). The foremost factors were tartaric acid, PVP K-30, sodium polyacrylate NP-700, aluminium glycinate, glycerin, and EDTA-2Na. Conclusion The optimal prescription has easy ductility, uniform paste, and moderate adhesion, and it could provide the foundation for the development of new prescription of Gui-Shao Cataplasm; The PCA could be a reference for the Chinese medicine research of multiple indexes.
Objective To establish a comprehensive evaluation method based on principal component analysis(PCA) for the study of multiple indexes of Chinese medicine and to choose the best preparation technology of Gui-Shao Cataplasm with this method. Methods The appearance description, initial bonding strength, endurance bonding strength, and peel strength were taken as indexes, the total factor scores were calculated using PCA and were employed to evaluate the quality of Gui-Shao Cataplasm, the uniform design test was used to optimize the amounts of sodium polyacrylate NP-700, aluminium glycinate, glycerin, etc. for Gui-Shao Cataplasm. Results The optimal ratio of the prescription was as following: sodium polyacrylate NP-700-PVP K-30-aluminium glycinate-EDTA- 2Na-tartaric acid-glycerin-extract(8∶1∶0.25∶0.07∶0.15∶25∶20). The foremost factors were tartaric acid, PVP K-30, sodium polyacrylate NP-700, aluminium glycinate, glycerin, and EDTA-2Na. Conclusion The optimal prescription has easy ductility, uniform paste, and moderate adhesion, and it could provide the foundation for the development of new prescription of Gui-Shao Cataplasm; The PCA could be a reference for the Chinese medicine research of multiple indexes.
引文
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[2]李伟泽,赵宁,师湘月,等.胶束增溶技术在含挥发油中药巴布剂试生产中的应用研究[J].中草药,2013,44(19):2677-2682.
[3]张晓星,吴铁雄,周莉,等.基于主成分分析的平原26省林业发展水平评价[J].中南林业科技大学,2010,4(4):10-13.
[4]马天慧,黄春毅.应用主成分分析方法对区域规划指标体系进行降维及评价[J].科技情报开发与经济,2009,19(29):125-127.
[5]刘仁权.SPSS统计软件[M].北京:中国中医药出版社,2007.
[6]李振皓,刘培,钱大玮,等.主成分分析用于香附四物汤效应部位体外经皮渗透的研究[J].药学学报,2013,48(6):933-939.
[7]王璐璐,张雁,鞠建明,等.桂芍巴布贴提取纯化工艺研究[J].时珍国医国药,2015,26(8):1922-1924.
[8]史亚军,袁普卫,王卉,等.星点设计-效应面优选消肿巴布剂的制备处方及初步临床疗效评价[J].中药材,2014,37(9):1665-1669.
[9]张传辉,贾成,李微,等.三七接骨凝胶膏剂的制备及体外透皮特性研究[J].中草药,2015,46(5):654-664.
[10]于燕燕.祖师麻有效部位巴布剂的药学研究[D].南京:南京中医药大学,2007.
[11]富志军,祝星,陈笑.挥发油包合对温脐巴布剂质量的影响[J].中成药,2014,36(10):2083-2087.
[12]张洪兵,朱雪瑜,张铁军,等.星点设计-效应面优化法优选复方止痛巴布剂基质处方[J].中草药,2013,44(8):985-988.
[13]顾治平,王恒斌,阙瑞艳,等.中药巴布剂的基质:中国,CN 103040792 A[P].2013-04-17.
[14]林海明.如何用SPSS快速计算主成分的结果[J].统计与决策,2011(12):152-154.
[15]蒋海峰,欧阳臻,宿树兰,等.基于主成分分析和相似度分析的乳香药材质量评价研究[J].中药材,2011,34(6):904-911.
[16]任荣军,夏新华.均匀设计法优选肿节风的醇沉工艺[J].中成药,2009,31(7):1118-1120.
[17]林媛媛,刘静,王冬梅,等.Box-Behnken试验设计法优化宝泻灵凝胶膏剂处方及其体外透皮特性研究[J].中草药,2014,45(9):1238-1244.
[18]董薇薇.清紫巴布剂的药学研究[D].合肥:安徽中医药大学,2013.
[19]马红.扶正益肺巴布膏的药学研究[D].北京:北京中医药大学,2006.
[20]宋信莉,刘文.正交试验法优选老鹳草巴布剂基质配方[J].中国实验方剂学杂志,2012,18(20):52-54.
[21]汪小根,邹玉繁,陈瑜珍.均匀设计法筛选广藿香有效部位巴布剂的基质处方[J].中国中药杂志,2008,33(6):638-641.
[22]薛宝娟,龙志贤,王玉蓉.均匀设计法优选玄麝止痛巴布剂基质配方研究[J].中药新药与临床药理,2008,19(6):499-501.
[23]张玲,刘艺萍,余伟民,等.双乌黑蚁巴布剂的制备及稳定性考查[J].中成药,2010,32(10):1803-1806.
[24]杜松云.喘敷灵巴布剂的药学研究[D].武汉:湖北中医学院,2007.