一株耐盐耐氧反硝化菌MCW148的分离鉴定及其脱氮特性
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摘要
从渤海某海水养殖场(北美白对虾)底泥中分离到1株耐盐高效好氧反硝化细菌MCW148,经过对其形态特征、生理生化以及16S r DNA序列分析,将该菌株初步鉴定为巨大芽孢杆菌(Bacillus megaterium)。进一步研究表明,菌株MCW148的最适碳源为葡萄糖,最适培养温度为35℃,最适pH为6。在最适条件下,菌株MCW148在12 h对NO3--N的去除率为62.4%。
A aerobic denitrification bacterial strain MCW148 with salt-tolerance and high denitrification ability was isolated from sediment of shrimp pond in the Bohai Sea. Results indicated this strain was identified as Bacillus megaterium according to its morphology, biochemical properties and 16 S r DNA sequence analysis. Further study indicated the optimal carbon was glucose, and the optimal pH and temperature for cell growth and denitrification were 6.0 and 35 ℃, respectively. And the NO_3~--N removal rate of the strain MCW148 was 62.4% in the optimal conditions for 12 h.
A aerobic denitrification bacterial strain MCW148 with salt-tolerance and high denitrification ability was isolated from sediment of shrimp pond in the Bohai Sea. Results indicated this strain was identified as Bacillus megaterium according to its morphology, biochemical properties and 16 S r DNA sequence analysis. Further study indicated the optimal carbon was glucose, and the optimal pH and temperature for cell growth and denitrification were 6.0 and 35 ℃, respectively. And the NO_3~--N removal rate of the strain MCW148 was 62.4% in the optimal conditions for 12 h.
引文
[1]任华峰,姜天翔,邱金泉,等.复合微生物菌剂在海水养殖废水净化中的试验研究[J].环境污染与防治,2015(7):111.
[2]ROBERTON L A,KUENEN J G.Aerobic denitrification:A controversy revived[J].Archives of Microbiology,1984,139(5):351-354.
[3]李彦芹,李春青,管鹏悦,等.一株好氧反硝化菌的分离鉴定及脱氮条件研究[J].环境科学与技术,2015,38(7):33-37.
[4]郑喜春,郭晓军,姚娜,等.反硝化芽孢杆菌的筛选鉴定及反硝化特性[J].生态学杂志,2012,31(6):1447-1452.
[5]张凯,雷梦婕,胡国元,等.好氧反硝化细菌WIT-1的分离鉴定及其脱氨氮特性[J].武汉工程大学学报,2011,33(11):14-18.
[6]马放,张佳,张献旭,等.生物陶粒反应器中好氧反硝化细菌的脱氮研究[J].中国环境科学,2007,27(4):529-533.
[7]吴智导,黄少斌,卢乐.曝气生物滤池处理含硝态氮废水的研究[J].水处理技术,2012,38(1):80-83.
[8]于爱茸,李尤,愈吉安.一株耐氧反硝化细菌的筛选及脱氮特性研究[J].微生物学杂志,2005,25(3):77-81.
[9]杜立刚,张聪琳,陈宝玉.生物反硝化生物脱氮技术研究进展[J].能源与环境,2015(4):66-68.
[10]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001.
[11]KIM J K,PARK K J,CHO K S.Aerobic nitrification denitrification by heterotrophic Bacillus strains[J].Bioresource Technology,2005,96:1897-1906.
[12]丁炜,朱亮,徐京,等.好氧反硝化菌及其在生物处理与修复中的应用研究进展[J].应用与环境微生物学报,2011,17(6):923-929.
[13]王弘宇,马放,苏俊峰,等.不同碳源和碳氮比对一株好氧反硝化细菌脱氮性能的影响[J].环境科学学报,2007,27(6):968-972.
[14]王弘宇,马放,苏俊峰,等.含硝氮废水的好氧反硝化处理及其系统微生物群落动态分析[J].环境科学,2007,28(12):2856-2860.
[15]乔南,陈瑞佳,于大禹.硅藻土负载异氧硝化-好氧反硝化菌的脱氮性能[J].化工进展,2015,34(5):1459-1465.
[16]车鉴,徐牧,阳桂菊,等.硅藻土固定化颗粒污泥对海水养殖废水除氨性能[J].环境工程学报,2014,8(12):5318-5322.
[2]ROBERTON L A,KUENEN J G.Aerobic denitrification:A controversy revived[J].Archives of Microbiology,1984,139(5):351-354.
[3]李彦芹,李春青,管鹏悦,等.一株好氧反硝化菌的分离鉴定及脱氮条件研究[J].环境科学与技术,2015,38(7):33-37.
[4]郑喜春,郭晓军,姚娜,等.反硝化芽孢杆菌的筛选鉴定及反硝化特性[J].生态学杂志,2012,31(6):1447-1452.
[5]张凯,雷梦婕,胡国元,等.好氧反硝化细菌WIT-1的分离鉴定及其脱氨氮特性[J].武汉工程大学学报,2011,33(11):14-18.
[6]马放,张佳,张献旭,等.生物陶粒反应器中好氧反硝化细菌的脱氮研究[J].中国环境科学,2007,27(4):529-533.
[7]吴智导,黄少斌,卢乐.曝气生物滤池处理含硝态氮废水的研究[J].水处理技术,2012,38(1):80-83.
[8]于爱茸,李尤,愈吉安.一株耐氧反硝化细菌的筛选及脱氮特性研究[J].微生物学杂志,2005,25(3):77-81.
[9]杜立刚,张聪琳,陈宝玉.生物反硝化生物脱氮技术研究进展[J].能源与环境,2015(4):66-68.
[10]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001.
[11]KIM J K,PARK K J,CHO K S.Aerobic nitrification denitrification by heterotrophic Bacillus strains[J].Bioresource Technology,2005,96:1897-1906.
[12]丁炜,朱亮,徐京,等.好氧反硝化菌及其在生物处理与修复中的应用研究进展[J].应用与环境微生物学报,2011,17(6):923-929.
[13]王弘宇,马放,苏俊峰,等.不同碳源和碳氮比对一株好氧反硝化细菌脱氮性能的影响[J].环境科学学报,2007,27(6):968-972.
[14]王弘宇,马放,苏俊峰,等.含硝氮废水的好氧反硝化处理及其系统微生物群落动态分析[J].环境科学,2007,28(12):2856-2860.
[15]乔南,陈瑞佳,于大禹.硅藻土负载异氧硝化-好氧反硝化菌的脱氮性能[J].化工进展,2015,34(5):1459-1465.
[16]车鉴,徐牧,阳桂菊,等.硅藻土固定化颗粒污泥对海水养殖废水除氨性能[J].环境工程学报,2014,8(12):5318-5322.