摘要
目的研究我国水产品中分离的副溶血性弧菌耐药性、产毒特性及遗传特征。方法对我国26个省、直辖市和自治区水产品中分离的1 137株副溶血性弧菌进行耐药性分析和毒力基因检测,并用脉冲场凝胶电泳方法比对产毒菌株的遗传特征。结果 1 137株副溶血性弧菌中有254株(22.3%)受试菌株对测定的12种抗生素全部敏感,883株(77.7%)对测定的抗生素呈现不同程度耐药。所有受试菌株对左氧氟沙星和庆大霉素抗生素敏感,主要耐受的抗生素为氨苄西林(53.7%,611/1 137)、阿莫西林/克拉维酸(46.3%,526/1 137)和头孢唑林(24.2%,275/1 137)。1 137株副溶血性弧菌分离株全部检出tlh基因,有3株检出tdh基因,有19株检出trh基因,其中2株同时检出tdh和trh基因。20株产毒菌株遗传特征分散,与其食品来源和耐药谱之间也无密切关联,其中4株对测定的抗生素均敏感,其余菌株主要对青霉素类和头孢类抗生素耐药。结论我国水产品中副溶血性弧菌对青霉素类抗生素普遍耐药,毒力基因携带率较低,产毒菌株遗传特征多样。
Objective To evaluate antimicrobial resistance,virulence and genetic characteristics of Vibrio parahaemolyticus isolated from aquatic products in China. Methods The susceptibility of 1 137 isolates of Vibrio parahaemolyticus from 26 provinces was determined,and virulence genes including tlh,tdh and trh were detected. The genetic characteristics of Vibrio parahaemolyticus were analyzed by pulsed field gel electrophoresis method. Results Two hundred and fifty-four strains( 22. 3%) were susceptible to all the antibiotics tested and 883 strains( 77. 7%) presented resistance to single or multiple antibiotics. All strains were susceptible to gentamicin and levofloxacin. The highest resistance rates were ampicillin( 53. 7%,611/1 137),amoxicillin/clavulanic acid( 46. 3%,526/1 137) and cefazolin( 24. 2%,275/1 137). All the 1 137 isolates were positive for tlh gene,including 3 isolates were positive for tdh gene and 19 isolates were positive for trh gene. Twenty strains with virulence genes were resistance to mainly penicillins and cephalosporin and 4 strains of them were snsceptible to all the antibiotics tested. There was no certain relation between the source and drug-resistant spectrum in these strains with virulence genes,and they displayed different genetic characteristics. Conclusion Vibrio parahaemolyticus isolated from aquatic products were resistant to penicillins widely in China,but the rate of virulence genes of strains isolated from food was lower than clinical. The genetic characteristics of virulent strains were varied.
引文
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