基于化学分析与抗氧化活性的余甘子提取工艺效果对比
|
摘要
目的:确定余甘子药材的最佳提取工艺条件。方法:以没食子酸、没食子儿茶素、表儿茶素、柯里拉京、鞣花酸5个成分含量为考察指标,采用正交设计试验分别考察了药材料液比、提取时间和提取次数对超声提取、回流提取及减压提取的影响,得到3种提取方法的最佳工艺,并基于抗DPPH氧化活性进行3种最佳提取工艺的生物活性比较。结果:余甘子超声提取的最佳工艺为加入8倍量水,超声3次,每次20min;常压回流提取的最佳提取工艺为加入12倍量水,提取2次,每次1h。减压回流提取的最佳提取工艺为加8倍量水,提取3次,每次2h。且常压回流提取物具有最优的抗DPPH氧化活性。结论:3种最佳提取工艺均具有好的提取效果,但相比超声提取,常压回流与减压回流提取的提取物抗氧化活性更高,因此回流提取更加适合余甘子药材的提取。
Objective: To determine the optimum extraction conditions of Phyllanthis Fructus. Methods: The content of 5 components of gallic acid, gallic catechins, epicatechin, coriagin and tannic acid were taken as indexes. Orthogonal design was used to investigate the effects of the ratio of liquid to drug, extraction time and extraction times on ultrasonic extraction, atmospheric reflux extraction and decompression reflux extraction. The best process of three extraction methods was obtained and the bioactivity of the three best extraction processes based on the anti DPPH oxidation activity was compared. Results: The optimum ultrasonic extraction of Phyllanthus emblica was: adding 8 times of water, three times of ultrasound, 20 minutes each time; the optimum extraction process of atmospheric reflux extraction was adding 12 times of water, extracting twice, 1 hour each time. The best optimum extraction process of decompressing reflux extraction was adding 8 times of water, extracting 3 times, 2 hours each time. And the atmospheric reflux extraction had best anti DPPH oxidation activity. Conclusion: Three of the optimum extraction process obtained has good extraction effect, but compared with ultrasonic extraction, atmospheric reflux extraction and decompression reflux extraction have higher antioxidant activity, so the reflux extraction is more suitable for Phyllanthis Fructus.
Objective: To determine the optimum extraction conditions of Phyllanthis Fructus. Methods: The content of 5 components of gallic acid, gallic catechins, epicatechin, coriagin and tannic acid were taken as indexes. Orthogonal design was used to investigate the effects of the ratio of liquid to drug, extraction time and extraction times on ultrasonic extraction, atmospheric reflux extraction and decompression reflux extraction. The best process of three extraction methods was obtained and the bioactivity of the three best extraction processes based on the anti DPPH oxidation activity was compared. Results: The optimum ultrasonic extraction of Phyllanthus emblica was: adding 8 times of water, three times of ultrasound, 20 minutes each time; the optimum extraction process of atmospheric reflux extraction was adding 12 times of water, extracting twice, 1 hour each time. The best optimum extraction process of decompressing reflux extraction was adding 8 times of water, extracting 3 times, 2 hours each time. And the atmospheric reflux extraction had best anti DPPH oxidation activity. Conclusion: Three of the optimum extraction process obtained has good extraction effect, but compared with ultrasonic extraction, atmospheric reflux extraction and decompression reflux extraction have higher antioxidant activity, so the reflux extraction is more suitable for Phyllanthis Fructus.
引文
[1]刘延泽,李海霞,许利嘉,等.药食兼用余甘子的现代研究概述及应用前景分析.中草药,2013,44(12):1700-1706
[2]崔炳权,黄伟侨,林元藻.余甘子抗疲劳、抗氧化作用实验研究.中国现代中药,2008,10(6):26-28
[3]罗春丽.余甘子对肿瘤细胞抑制作用及免疫调节的研究.中国实验方剂学杂志,2010,16(13):155-158
[4]岑志芳,张继平,李海燕,等.余甘子提取物对尿酸钠诱导大鼠急性痛风性关节炎的作用研究.中国药房,2011,22(47):4425-4427
[5]曹维,朱建梅,文洁,等.余甘子有效成分提取工艺研究.中药材,2013,36(5):812-815
[6]宋琳琳,沙靖全,张磊,等.余甘子中总黄酮提取工艺研究.辽宁中医药大学学报,2016,18(4):42-44
[7]吴丹玲,郑必胜.余甘子抗氧化活性成分的研究.安徽农业科学,2010,38(24):13045-13046
[8]陈敬,温庆果,刘超,等.正交设计与响应面法优化壳聚糖对莲子心提取液除杂工艺对比研究.中草药,2012,43(11):2183-2188
[9]郭莹,虞夏晖,陈碧宵,等.望江南子不同溶剂提取物体外抗氧化活性及抗脑缺血性损伤作用.中华中医药杂志,2018,33(11):5168-5171
[10]姚喆.藏药三果汤散抗氧化有效成分研究.成都:成都中医药大学,2011
[11]刘刚,孙磊,乔善义.鞣质的分离和分析方法研究进展.国际药学研究杂志,2013,40(2):172-176,202
[12]邢晶晶,曹婷婷,杨帆,等.鞣质类化合物研究的进展情况.黑龙江医药,2011,35(5):776-780
[2]崔炳权,黄伟侨,林元藻.余甘子抗疲劳、抗氧化作用实验研究.中国现代中药,2008,10(6):26-28
[3]罗春丽.余甘子对肿瘤细胞抑制作用及免疫调节的研究.中国实验方剂学杂志,2010,16(13):155-158
[4]岑志芳,张继平,李海燕,等.余甘子提取物对尿酸钠诱导大鼠急性痛风性关节炎的作用研究.中国药房,2011,22(47):4425-4427
[5]曹维,朱建梅,文洁,等.余甘子有效成分提取工艺研究.中药材,2013,36(5):812-815
[6]宋琳琳,沙靖全,张磊,等.余甘子中总黄酮提取工艺研究.辽宁中医药大学学报,2016,18(4):42-44
[7]吴丹玲,郑必胜.余甘子抗氧化活性成分的研究.安徽农业科学,2010,38(24):13045-13046
[8]陈敬,温庆果,刘超,等.正交设计与响应面法优化壳聚糖对莲子心提取液除杂工艺对比研究.中草药,2012,43(11):2183-2188
[9]郭莹,虞夏晖,陈碧宵,等.望江南子不同溶剂提取物体外抗氧化活性及抗脑缺血性损伤作用.中华中医药杂志,2018,33(11):5168-5171
[10]姚喆.藏药三果汤散抗氧化有效成分研究.成都:成都中医药大学,2011
[11]刘刚,孙磊,乔善义.鞣质的分离和分析方法研究进展.国际药学研究杂志,2013,40(2):172-176,202
[12]邢晶晶,曹婷婷,杨帆,等.鞣质类化合物研究的进展情况.黑龙江医药,2011,35(5):776-780