糖尿病大鼠胃黏膜细胞色素C异常表达及大黄素调控
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摘要
目的:探讨糖尿病胃病的相关机制及大黄素干预。方法:SD大鼠随机分为对照组(正常组)、实验组(糖尿病组)与大黄素组,实验组应用腹腔一次性注射链脲佐菌素(streptozotocin,STZ)建立糖尿病大鼠模型,造模成功10周后免疫组织化学检测胃黏膜腺细胞细胞色素C(cytc)的表达情况。结果:①实验组和大黄素组大鼠于造模后均出现多尿、多饮、多食症状,体重减轻,平均体重明显低下对照组(P<0.05);平均血糖浓度显著高于对照组(P<0.05)等糖尿病症状。但应用大黄素干预组大鼠的血糖浓度显著低于实验组。②实验组cyt c蛋白阳性表达的胃黏膜细胞数明显高于对照组(P<0.05;大黄素组cyt c蛋白阳性表达的胃黏膜细胞数明显少于实验组(P<0.05)。结论:糖尿病引起大鼠胃黏膜细胞高表达cyt c蛋白,导致细胞死亡增加,参与糖尿病胃病的发生,而大黄素可降低胃黏膜细胞cyt c蛋白表达,从而缓解糖尿病胃病。
Objective:To investigate the pathegenetic mechanism and effect of emodin on gastric disturbance of diabetic rats.Methods:SD rats were randomly divided into control group,diabetic group and emodin group.Ten weeks later,expression of cytochrome C in gastric mucosa was detected by immunohistochemistry.Results:(Din diabetic group and emodin group,typical symptoms of diabetes appeared,the average weight was significantly lower(P< 0.05),and the blood glucose was significantly higher than those in control group(P< 0.05).(2) Compared with rats in control group,the cytochrome C in diabetic group was significantly higher {P < 0.05).Compared with diabetic group,the cytochrome C in emodin group was significantly lower(P < 0.05).Conclusion:Diabetes causes high expression of cytochrome C in gastric mucosal cells,which induces cell death and gastric disturbance of diabetic rats.Emodin may decrease cytochrome C in gastric mucosa and therefore,alleviate the gastric disturbance of betic rats.
Objective:To investigate the pathegenetic mechanism and effect of emodin on gastric disturbance of diabetic rats.Methods:SD rats were randomly divided into control group,diabetic group and emodin group.Ten weeks later,expression of cytochrome C in gastric mucosa was detected by immunohistochemistry.Results:(Din diabetic group and emodin group,typical symptoms of diabetes appeared,the average weight was significantly lower(P< 0.05),and the blood glucose was significantly higher than those in control group(P< 0.05).(2) Compared with rats in control group,the cytochrome C in diabetic group was significantly higher {P < 0.05).Compared with diabetic group,the cytochrome C in emodin group was significantly lower(P < 0.05).Conclusion:Diabetes causes high expression of cytochrome C in gastric mucosal cells,which induces cell death and gastric disturbance of diabetic rats.Emodin may decrease cytochrome C in gastric mucosa and therefore,alleviate the gastric disturbance of betic rats.
引文
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13.宁建文,季峰,骆丹东,等.大黄素对重症急性胰腺炎肠黏膜细胞凋亡和血清瘦素表达的影响[J].中西医结合学报,2009,7(12):1 167-1 173.
2.郭海平,高桦,邱明才.吡格列酮对糖尿病大鼠胃黏膜肥大细胞和单核细胞趋化蛋白-1表达的影响[J].实用医学杂志,2010,26(17):3112~3114.
3.王少真,陈碧宵,徐延光,等.2型糖尿病与胃黏膜损伤的相关性分析[J].天津医药,2008,36(11):863-865.
4.方厚华.医学实验模型动物[M].北京:军事医学科学出版社,2002,57-58.
5.郭丽,林鹭平.链脲佐菌素诱导大鼠糖尿病动物模型的建立[J].中国医药研究与临床,2006,4(5):4-5.
6.沈亚非,徐焱成.链脲佐菌素诱导实验性糖尿病模型建立的研究[J].实用诊断与治疗杂志,2005,19(2):79-80.
7.任蓓.潘澄,林鹏,等.高糖诱发氧化应激导致血管内皮细胞凋亡机制研究[J].海峡药学,2013,25(6):268-271.
8.吴学平,金晓梅,彭彦霄,等.凋亡相关基因Bcl-2、Bax及细胞色素C在糖尿病胃组织壁细胞中的表达[J],中囤组织化学与细胞化学杂志,2011,20(2):1130-134.
9.Mizuta T,Shimizu S,MatsuokaY.et al.A Bax/Bak-independent M echanism of Cytochrome Release[J].BiolChem,2007,282(22):16623-16630.
10.Chen Q,Hoppel C L,Lesnefiky EL,et al.Blockade of electron transport before cardiac ischemia with the reversible inhibitor amobarbital protects rat heart mitochondria[J].JPET,2006,316(1):200-207.
11.Petrosillo G.Ruggiiero F M,Paradies G,et al.Role of reactive oxygen species and cardiolipin in the release of cytochrome C from mitochondria[J].FASEB J,2003,17(15):2202-2208.
12.陆英杰.连至诚,钟毅,等.30周糖尿病模型大鼠胃排空异常及胃黏膜细胞凋亡的实验研究[J].佛山科学技术学院学报自然科学版,2009,27(2):69-71.
13.宁建文,季峰,骆丹东,等.大黄素对重症急性胰腺炎肠黏膜细胞凋亡和血清瘦素表达的影响[J].中西医结合学报,2009,7(12):1 167-1 173.