茵胆平肝胶囊对急性酒精性肝脏炎症损伤和氧化应激的保护作用研究
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摘要
探讨茵胆平肝胶囊(YDPG)对急性酒精性肝脏炎症损伤和氧化应激的保护作用,该研究将雄性SD大鼠60只,随机分为正常对照组,模型组,茵胆平肝胶囊低、中、高剂量组(YDPG-L,YDPG-M,YDPG-H组),海王金樽阳性对照组(HWJZ组),每组10只。正常组灌胃给予等量生理盐水,其余各组均灌胃给予50%乙醇5 g·kg~(-1),乙醇灌胃1 h后,模型组给予生理盐水,YDPG-L,YDPG-M,YDPG-H组分别给予0.15,0.3,0.6 g·kg~(-1),HWJZ组给予0.3 g·kg~(-1),乙醇及药物连续给予10 d,每天1次。末次灌胃并禁食8 h后,取肝组织进行石蜡切片HE染色观察肝组织病理改变;检测大鼠血清中ALT,AST,ALP,TB,TP,LDH的含量;ELISA法检测血清中IL-1β,TNF-α,IL-10和IL-4的含量;同时检测肝组织中SOD,MDA,GSH,GSP-Px的含量。结果显示,给药后均不同程度缓解了酒精导致的肝细胞损伤;降低了ALT,AST,ALP,LDH的活性;下调了血清中炎症因子TNF-α和IL-1β的表达,上调了炎症因子IL-4和IL-10的表达;升高了SOD,GSH,GSH-Px活性,降低MDA含量。以上结果表明YDPG对急性酒精性炎症损伤和氧化应激反应具有保护作用。
To investigate the protective effect of Yindan Pinggan Capsules(YDPG)on acute alcoholic liver inflammatory injury and oxidative stress,sixty male Sprague-Dawley rats were divided into 6 groups randomly:normal group,model group,YDPG-L group,YD-PG-M group,YDPG-H group and Haiwang Jinzun(HWJZ)group.Except for the normal rats,while the remaining rats were administered orally with alcohol(50%,5 g·kg~(-1)).One hour after drinking,the rats in normal or model group were administered orally with PS,while the remaining rats were separately given YDPG(0.15,0.3,0.6 g·kg~(-1))or HWJZ(0.3 g·kg~(-1))for 10 days.After the last intragastric administration and fasting for 8 h,the HE staining was used to observe the pathological changes.The serum was used to determine the content of ALT,AST,ALP,TB,TP,LDH.The expressions of IL-1β,TNF-α,IL-10 and IL-4 in serum were detected by enzyme-linked immuno sorbent assay(ELISA).The content of SOD,MDA,GSH,GSP-Px in liver were determined.The results showed that both YDPGand HWJZ alleviated the liver injury induced by alcohol,decreased the activities of ALT,AST,ALP and LDH,down-regulated the expressions of TNF-αand IL-1β,up-regulated the expressions of IL-4 and IL-10;increased the activities of SOD,GSH,GSH-Px,but decreased the content of MDA.Therefore,the above results indicated that YDPG could protect the liver from alcoholic injury.
To investigate the protective effect of Yindan Pinggan Capsules(YDPG)on acute alcoholic liver inflammatory injury and oxidative stress,sixty male Sprague-Dawley rats were divided into 6 groups randomly:normal group,model group,YDPG-L group,YD-PG-M group,YDPG-H group and Haiwang Jinzun(HWJZ)group.Except for the normal rats,while the remaining rats were administered orally with alcohol(50%,5 g·kg~(-1)).One hour after drinking,the rats in normal or model group were administered orally with PS,while the remaining rats were separately given YDPG(0.15,0.3,0.6 g·kg~(-1))or HWJZ(0.3 g·kg~(-1))for 10 days.After the last intragastric administration and fasting for 8 h,the HE staining was used to observe the pathological changes.The serum was used to determine the content of ALT,AST,ALP,TB,TP,LDH.The expressions of IL-1β,TNF-α,IL-10 and IL-4 in serum were detected by enzyme-linked immuno sorbent assay(ELISA).The content of SOD,MDA,GSH,GSP-Px in liver were determined.The results showed that both YDPGand HWJZ alleviated the liver injury induced by alcohol,decreased the activities of ALT,AST,ALP and LDH,down-regulated the expressions of TNF-αand IL-1β,up-regulated the expressions of IL-4 and IL-10;increased the activities of SOD,GSH,GSH-Px,but decreased the content of MDA.Therefore,the above results indicated that YDPG could protect the liver from alcoholic injury.
引文
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[2]肖达民,李丹青,吴艳华.酒精性肝病的中医临床研究进展[J].中药新药与临床药理,2018,29(1):118.
[3]Mathews S,Xu M,Wang H,et al.Animals models of gastrointestinal and liver diseases.Animal models of alcohol induced liver disease:pathophysiology,translational relevance,and challenges[J].Am J Physiol Gastrointest Liver Physiol,2014,306(10):819.
[4]李远辉,黄挺章,郭圣奇,等.亚麻子提取物对D-半乳糖胺致小鼠急性肝损伤的保护作用[J].中国实验方剂学杂志,2015,21(16):139.
[5]Keane J,Gershon S,Wise R P,et al.Tuberculosis associated with infliximab,a tumor necrosis factor alpha-neutralizing agent[J].N Engl J Med,2001,345(15):1098.
[6]王洪岩,李鑫,徐有青.酒精性肝病发病机制研究进展[J].实用肝脏病杂志,2014,17(1):5.
[7]韦家河,焦雯,黄春佳,等.百香果果汁对D-半乳糖致衰老小鼠抗氧化作用的研究[J].中国药师,2018(4):581.
[8]常学文,王红玲.茵胆平肝胶囊治疗酒精性肝病湿热蕴结证41例[J].河南中医,2015,35(9):2101.
[9]王敏,龚先琼.茵胆平肝胶囊治疗酒精性肝病18例疗效观察[J].湖南中医杂志,2014,30(11):60.
[10]杨华升,金瑞,李秀惠.茵胆平肝胶囊治疗慢性乙型肝炎疗效观察[J].传染病信息,2006,19(4):199.