外源IAA对盐胁迫黄芩幼苗生长的生理效应
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摘要
以商洛道地药材黄芩1年生实生幼苗为供试材料,采用0.5 g/m L NaCl模拟盐胁迫处理盆栽黄芩幼苗,研究了不同质量浓度(0,2,4,6,8,10 mg/L)IAA处理和施加方法对盐胁迫下黄芩幼苗生理特性的影响。结果显示,在盐胁迫下,不同质量浓度IAA的不同施加方式处理黄芩幼苗与对照组CK2(0.5 g/m L NaCl)相比,MDA含量明显低于对照CK2,而叶绿素、脯氨酸、可溶性蛋白、可溶性糖、POD和CAT酶活性均呈现不同程度升高趋势,其中以叶面喷施6 mg/L IAA处理效果最佳,叶绿素、脯氨酸、可溶性蛋白、可溶性糖、POD活性、CAT酶活性、丙二醛含量分别达3.33 mg/g,10.65μg/g,2.07 mg/g,9.05%,23.90 U/g,4.69 U/g,7.32 nmol/g,较对照CK2分别增加285%,197%,88%,147%,77%,227%,-61%。综合以上结果表明,通过施加外源物质IAA可以提高黄芩幼苗的耐盐性,且以叶面喷施6 mg/L的IAA作用效果最佳。
The seedlings of Scutellaria baicalensis were treated with the concentration of 0.5 g/m L NaCl simulated salt stress. The effects of different concentrations(0, 2, 4, 6, 8,10 mg/L)on the physiological characteristics of Scutellaria baicalensis Georgi seedlings under salt stress were studied. The results showed that under salt stress, the content of MDA in Scutellaria baicalensis seedlings was significantly lower than that of control group CK2(0.5 g/m L NaCl), and the content of chlorophyll, proline, soluble protein, soluble sugar,POD and CAT enzyme activity increased in different degrees compared with the control group CK2, the effect of spraying 6 mg/L IAA was the best. The content of chlorophyll, proline, soluble protein, soluble sugar, POD, CAT enzyme activity, MDA content were 3.33 mg/g,10.65 μg/g, 2.07 mg/g, 9.05%, 23.90 U/g, 4.69 U/g, 7.32 nmol/g, the increase was 285%, 197%, 88%, 147%, 77%, 227%,-61%,respectively. The above results show that the salt tolerance of Scutellaria baicalensis Georgi seedlings can be improved by applying exogenous IAA, and the effect of spraying 6 mg/L on IAA is the best.
The seedlings of Scutellaria baicalensis were treated with the concentration of 0.5 g/m L NaCl simulated salt stress. The effects of different concentrations(0, 2, 4, 6, 8,10 mg/L)on the physiological characteristics of Scutellaria baicalensis Georgi seedlings under salt stress were studied. The results showed that under salt stress, the content of MDA in Scutellaria baicalensis seedlings was significantly lower than that of control group CK2(0.5 g/m L NaCl), and the content of chlorophyll, proline, soluble protein, soluble sugar,POD and CAT enzyme activity increased in different degrees compared with the control group CK2, the effect of spraying 6 mg/L IAA was the best. The content of chlorophyll, proline, soluble protein, soluble sugar, POD, CAT enzyme activity, MDA content were 3.33 mg/g,10.65 μg/g, 2.07 mg/g, 9.05%, 23.90 U/g, 4.69 U/g, 7.32 nmol/g, the increase was 285%, 197%, 88%, 147%, 77%, 227%,-61%,respectively. The above results show that the salt tolerance of Scutellaria baicalensis Georgi seedlings can be improved by applying exogenous IAA, and the effect of spraying 6 mg/L on IAA is the best.
引文
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[14]李小玲,华智锐.外源Ca2+对盐胁黄芩种子的萌发及生理特性影响[J].贵州农业科学,2016,44(11):145-148.
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[17]王立红,李星星,孙影影,等.外源水杨酸对Na Cl胁迫下棉花幼苗生长生理特性的影响[J].西北植物学报,2017,37(1):154-162.
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[21]尚宏芹,刘兴坦.6-BA浸种对Hg Cl2胁迫下小麦种子萌发和幼苗生长的影响[J].麦类作物学报,2015,35(10):1438-1444.
[22]ZHOU J M,DANG Z.Enhanced phytoextraction of heavy metal contaminated soil by chelating agents and auxin indole-3-acetic acid[J].Environmental Science,2007,28(9):2085-2088.
[23]华智锐,李小玲.外源硅对盐胁迫下黄芩幼苗生理生化特性的影响[J].江西农业科学,2018,30(3):23-27.
[24]刘拴成,曹兴明,穆俊祥,等.外源激素对盐胁迫下番茄种子萌发及幼苗生长的影响[J].种子,2016,35(12):94-98.
[2]郭菊梅,陈红刚,曹瑞,等.氯化钠浓度对黄芩幼苗生长情况及生理指标的影响[J].中国药房,2014,25(47):4497-4499.
[3]LI J T,ZHAO P P,QIU Z H.Effects of exogenous hydrogen peroxide on the physiological index of wheat seedlings under salt stress[J].Acta Botanica Boreali-Occidentalia Sinica,2012,32(9):1796-1801.
[4]DUAN J,LI J,KANG Y.Exogenous spermidine affects polyamine metabolism in salinity-stressed Cucumis sativus roots and enhances short-term salinity tolerance[J].Journal of Plant Physiology,2008,165(15):1620-1635.
[5]苗丽,巩彪,聂文靖,等.外源IAA对Na HCO3胁迫下黄瓜幼苗光合特性和抗氧化系统的影响[J].植物生理学报,2014,50(6):765-771.
[6]任晓燕,周鹏,安渊.喷施IAA对紫花苜蓿幼苗铝毒害的缓解作用[J].草业科学,2014,31(7):1323-1329.
[7]李亚萍,彭燕.IAA改善PEG处理下白三叶幼苗叶片抗氧化保护和渗透调节能力[J].草业科学,2017,34(11):2295-2302.
[8]李晓宇,穆春生.盐碱胁迫及外源植物激素对小麦和羊草生长发育的影响[J].草地学报,2017,25(2):257-260.
[9]王平,陈奇,陈东杰,等.外源添加IAA对铝胁迫下黑大豆根系生长的影响[J].大豆科学,2013,32(5):650-654.
[10]何帅.黄芩的研究进展[C]//中国商品学会第五届全国中药商品学术大会论文集.西安:西安电子科技大学,2017:219-228.
[11]李小玲,华智锐.外源ALA对盐胁迫下商洛黄芩生理特性的影响[J].江西农业学报,2016,28(2):70-73.
[12]江绪文,李贺勤.盐胁迫下黄芩种子萌发及幼苗对外源抗坏血酸的生理响应[J].植物生理学报,2015,51(2):166-170.
[13]李小玲,华智锐.外源脱落酸对盐胁迫下商洛黄岑生理特性的影响[J].江西农业学报,2017,29(7):36-39.
[14]李小玲,华智锐.外源Ca2+对盐胁黄芩种子的萌发及生理特性影响[J].贵州农业科学,2016,44(11):145-148.
[15]王平,陈东杰,李昆志,等.外源IAA增强丹波黑大豆抗铝性的生理机制[J].西北植物学报,2014,34(1):112-117.
[16]刘亚栋,张长峰.SA浸种对盐胁迫下小麦种子萌发和幼苗生长的影响[J].生命科学研究,2017,21(3):244-250.
[17]王立红,李星星,孙影影,等.外源水杨酸对Na Cl胁迫下棉花幼苗生长生理特性的影响[J].西北植物学报,2017,37(1):154-162.
[18]张志良,翟伟箐,李小方.植物生理实验指导[M].北京:高等教育出版社,2009:227-228.
[19]李奕松,姬谦龙,马兰青,等.植物生理学实验指导[M].北京:高等教育出版社,2016:11-13.
[20]赵世杰,史国安,董新纯.植物生理学实验指导[M].北京:中国农业科学技术出版社,2002:58-60.
[21]尚宏芹,刘兴坦.6-BA浸种对Hg Cl2胁迫下小麦种子萌发和幼苗生长的影响[J].麦类作物学报,2015,35(10):1438-1444.
[22]ZHOU J M,DANG Z.Enhanced phytoextraction of heavy metal contaminated soil by chelating agents and auxin indole-3-acetic acid[J].Environmental Science,2007,28(9):2085-2088.
[23]华智锐,李小玲.外源硅对盐胁迫下黄芩幼苗生理生化特性的影响[J].江西农业科学,2018,30(3):23-27.
[24]刘拴成,曹兴明,穆俊祥,等.外源激素对盐胁迫下番茄种子萌发及幼苗生长的影响[J].种子,2016,35(12):94-98.