诱导型一氧化氮合酶(iNOS)与神经源性膀胱的关系研究
摘要
<正>一氧化氮(NO)是高等动物细胞中的一种结构简单却非常重要的信号分子,目前对NO的研究涉及生理学、生物化学、分子遗传学、分子生物学、病理毒理学等多个学科,发现它在心脏、肺脏、肾脏、神经组织、胰腺、胃肠道发挥着非常重要的生理作用~([1,2]),但在膀胱组织中的研究还处于起步阶段。神经源性膀胱仍是国际性医学难题,常见于神经管发育缺陷或脊髓栓系综合征等,神经源性膀胱的基
引文
1 Tiwari M,Prasad S,Pandey AN,et al.Nitric oxide signaling during meiotic cell cycle regulation in mammalian oocytes[J].Front Biosci(Schol Ed),2017,9(3):307-318.
2 Fukami K,Kawabata A.Hydrogen sulfide and neuronal differentiation:Focus on Ca2+channels[J].Nitric Oxide,2015,46:50-54.DOI:10.1016/j.niox.2015.02.001.
3 Nasyrova RF,Ivashchenko DV,Ivanov MV,et al.Role of nitric oxide and related molecules in schizophrenia pathogenesis:biochemical,genetic and clinical aspects[J].Front Physiol,2015,6:139.DOI:10.3389/fphys.2015.00139.
4 Liu Y,Weiguang LI,Linyan HU,et al.Downregulation of nitric oxide by electroacupuncture against hypoxic-ischemic brain damage in rats via nuclear factor-κB/neuronal nitric oxide synthase[J].Mol Med Rep,2015,11(2):837.DOI:10.3892/mmr.2014.2879.
5 Nagano T.Practical methods for detection of nitric oxide[J].Luminescence,1999,14(6):283-290.DOI:10.1002/(SI-CI)1522-7243(199911/12)14:6<283:AID-BIO572>3.0.CO;2-G.
6 Eroglu E,Rost R,Bischof H,et al.Application of genetically encoded fluorescent nitric oxide(NO·)probes,the ge NOps,for real-time imaging of NO·signals in single cells[J].J Vis Exp,2017(121).DOI:10.3791/55486.
7 Monzon CM,Occhipinti R,Pignataro OP,et al.Nitric oxide reduces paracellular resistance in rat thick ascending limbs by increasing Na+and Cl-permeabilities[J].Am J Physiol Renal Physiol,2017,312(6):F1035.DOI:10.1152/ajprenal.00671.2016.
8 Keyser BM,Andres DK,Holmes WW,et al.Mustard gas inhalation injury:therapeutic strategy[J].Int J Toxicol,2014,33(4):271.DOI:10.1177/1091581814532959.
9李守林,陈维秀,陈雨历.神经源性膀胱组织中一氧化氮合酶表达的特点和意义[J].中华泌尿外科杂志,2010,31(10):695-699.DOI:10.3760/cma.j.issn.1000-6702.2010.10.014.Li SL,Chen WX,Chen YL.Biological significance of expression of nitric oxide synthases in neurogenic bladder[J].Chin J Urol,2010,31(10):695-699.DOI:10.3760/cma.j.issn.1000-6702.2010.10.014.
10李守林,刘晓东,姜俊海,等.血浆和尿中内皮素、一氧化氮在神经源性膀胱中的检测及意义[J].临床小儿外科杂志,2014(6):488-491.DOI:10.3969/j.issn.1671-6353.2014.06.005.Li SL,Liu XD,Jiang JH.The clinical significance of endothelin and nitrogen monoxidum of plasma and urinary in neurogenic bladder diseases[J].J Clin Ped Sur,2014(6):488-491.DOI:10.3969/j.issn.1671-6353.2014.06.005.
11 Austin PF,Casale AJ,Cain MP,et a1.Lipopolysaccharide and inflammatory cytokines cause an inducible nitric oxide synthase-dependent bladder smooth muscle librotic response[J].J Utol,2003,170:645-648.
12 Graziottin A,Gambini D.Anatomy and physiology of genital organs-women[J].Handb Clin Neurol,2015,130(4):39.DOI:10.1016/B978-0-444-63247-0.00004-3.
13 Fernandes VS,Ribeiro AS,Martínez-Sáenz A,et al.Underlying mechanisms involved in progesterone-induced relaxation to the pig bladder neck[J].Eur J Pharmacol,2014,723(1):246-252.
14 Lieb J,Kogan B,Das AK,et al.The effect of urine volume and nitric oxide on basal bladder blood flow:response to catheterization and drainage[J].Neurourol Urodyn,2015,20(1):115-124.
15 Govorov A,Kasyan G,Priymak D,et al.Tadalafil in the management of lower urinary tract symptoms:a review of the literature and current practices in Russia[J].Cent European J Urol,2014,67(2):167.DOI:10.5173/ceju.2014.02.art10.
16 Reitz A,Bretscher S,Knapp PA,et al.The effect of nitric oxide on the resting tone and the contractile behaviour of the external urethral sphincter:a functional urodynamic study in healthy humans[J].Eur Urol,2004,45(3):367-73.DOI:10.1016/j.eururo.2003.10.002.
17 Fernandes VS,Hernández M.The role of nitric oxide and hydrogen sulfide in urinary tract function[J].Basic Clin Pharmacol Toxicol,2016,119(S3):34-41.DOI:10.1111/bcpt.12565.
18范应中,王家祥,杨险峰.反射亢进型神经源性尿失禁膀胱逼尿肌中氮能神经及一氧化氮的变化对膀胱功能的影响[J].中国组织工程研究,2004,8(14):2694-2695.DOI:10.3321/j.issn:1673-8225.2004.14.067.Fan YZ,Wang JX,Yang XF.Influence of changes of nitric oxide synthase-immunoreactive nerve and level of nitric oxide in detrusor of bladder of huperreflexic neuropathic urinary incontinence on bladder function[J].J Chin Tis Eng Res,2004,8(14):2694-2695.DOI:10.3321/j.issn:1673-8225.2004.14.067.
19 Rocha JN.Effect of S-methyl-l-thiocitrulline dihydrochloride on rat micturition reflex[J].Int Braz J Urol,2016,42(5):1018-1027.DOI:10.1590/S1677-5538.IBJU.2015.0153.
20 Kakizaki H,Fraser MO,De Groat WC.Reflex pathways controlling urethral striated and smooth muscle function in the male rat[J].Am J Physiol,1997,272(5 Pt 2):R1647.
21 Moro C,Leeds C,Chess-Williams R.Contractile activity of the bladder urothelium/lamina propria and its regulation by nitric oxide[J].Eur J Pharmacol,2012,674(2-3):445-449.DOI:10.1016/j.ejphar.2011.11.020.
22 Johansson R,Andersson KE,Persson K.Nerve-mediated bladder contraction is impaired by cytokines:involvement of inducible nitric oxide synthase[J].Eur J Pharmacol,2003,476(3):221-227.DOI:0.1016/S0014-2999(03)02178-2.
23 Pereira ML,D'Ancona CA,Rojas-Moscoso JA,et al.Effects of nitric oxide inhibitors in mice with bladder outlet obstruction[J].Int Braz J Urol,2017,43(2):356-366.DOI:10.1590/S1677-5538.IBJU.2015.0441.
24 Birder LA,Wolf-Johnston A,Buffington CA,et al.Altered inducible nitric oxide synthase expression and nitric oxide production in the bladder of cats with feline interstitial cystitis[J].J Urol,2005,173(2):625-629.DOI:10.1097/01.ju.0000145900.22849.1d
25 Lemack GE,Zimmern PE,Vazquez D,et al.Altered response to partial bladder outlet obstruction in mice lacking inducible nitric oxide synthase[J].J Urol,2000,163(6):1981-1987.DOI:10.1097/00005392-200006000-00106.
26 Whitbeck C,Chichester P,Sokol R,et al.Role of nitric oxide in urinary bladder function:effect of L-arginine[J].Urol Int,2007,78(1):30-36.DOI:10.1159/000096931.
27 Miyaoka R,Mendes C,Schenka A,et al.BAY 41-2272,a soluble guanylate cyclase stimulator,relaxes isolated human ureter in a standardized in vitro model[J].Urology,2014,83(1):256.e1-256.e7.DOI:10.1016/j.urology.2013.09.005.
28 Ozen G,Gomez I,Daci A,et al.m PGES-1 inhibitor reduces human vascular tone by increasing PGI 2:a safer alternative to COXIBs:m PGES-1 inhibitor reduces vasoreactivity[J].Br J Pharmacol,2017.DOI:10.1111/bph.13939.
29 Salerno L,Sorrenti V,Di GC,et al.Progress in the development of selective nitric oxide synthase(NOS)inhibitors[J].Curr Pharm Des,2002,8(3):177-200.
30 Wang J,Hao L,Wang Y,et al.Inhibition of poly(ADP-ribose)polymerase and inducible nitric oxide synthase protects against ischemic myocardial damage by reduction of apoptosis[J].Mol Med Rep,2015,11(3):1768-1776.DOI:10.3892/mmr.2014.2977.
31 Bortolanza M,Cavalcantikiwiatkoski R,Padovanneto FE,et al.Glial activation is associated with l-DOPA induced dyskinesia and blocked by a nitric oxide synthase inhibitor in a rat model of Parkinson's disease[J].Neurobiol Dis,2015,73:377-387.DOI:10.1016/j.nbd.2014.10.017.
32 Broom L,Marinovamutafchieva L,Sadeghian M,et al.Neuroprotection by the selective i NOS inhibitor GW274150 in a model of Parkinson disease[J].Free Radic Biol Med,2011,50(5):633.DOI:10.1016/j.freeradbiomed.2010.12.026.
33 Mertas A,Duliban H,Szliszka E,et al.N-[3-(aminomethyl)benzyl]acetamidine(1400 W)as a potential immunomodulatory agent[J].Oxid Med Cell Longev,2014,2014(7):491214-491214.DOI:10.1155/2014/491214.
34 Nemec A,Pavlica Z,Petelin M,et al.Systemic use of selective i NOS inhibitor 1400W or non-selective NOS inhibitor lNAME differently affects systemic nitric oxide formation after oral porphyromonas gingivalis inoculation in mice[J].Arch Oral Biol,2010,55(7):509-514.DOI:10.1016/j.archoralbio.2010.04.003.
35 Garcin ED,Arvai AS,Rosenfeld RJ,et al.Anchored plasticity opens doors for selective inhibitor design in nitric oxide synthase[J].Nat Chem Biol,2008,4(11):700-7.DOI:10.1038/nchembio.115.
36 Yuji Tajiri,Valdemar Grill.Aminoguanidine exerts aβ-cell function-preserving effect in high glucose-culturedβ-cells(INS-1)[J].Int J Exp Diabetes Res,2015,1(2):111-119.
37 Lafioniatis A,Orfanidou MA,Papadopoulou ES,et al.Effects of the inducible nitric oxide synthase inhibitor aminoguanidine in two different rat models of schizophrenia[J].Behav Brain Res,2016,309:14-21.DOI:10.1016/j.bbr.2016.04.043.
38 Labuda CJ,Koblish M,Tuthill P,et al.Antinociceptive activity of the selective i NOS inhibitor AR-C102222 in rodent models of inflammatory,neuropathic and post-operative pain[J].Eur J Pain,2006,10(6):505-512.DOI:10.1016/j.ejpain.2005.07.004.
39 David D.Davey,Marc Adler,Damian Arnaiz,et al.Design,synthesis and activity of 2-imidazol-1-ylpyrimidine derived inducible nitric oxide synthase dimerization inhibitors[J].JMed Chem,2007,50(6):1146.DOI:10.1021/jm061319i.
40 Hangeland J,Friends TJ,Rossi KA,et al.Phenylimidazoles as potent and selective inhibitors of coagulation factor XIa with in vivo antithrombotic activity[J].J Med Chem,2014,57(23):9915-32.DOI:10.1021/jm5010607.
2 Fukami K,Kawabata A.Hydrogen sulfide and neuronal differentiation:Focus on Ca2+channels[J].Nitric Oxide,2015,46:50-54.DOI:10.1016/j.niox.2015.02.001.
3 Nasyrova RF,Ivashchenko DV,Ivanov MV,et al.Role of nitric oxide and related molecules in schizophrenia pathogenesis:biochemical,genetic and clinical aspects[J].Front Physiol,2015,6:139.DOI:10.3389/fphys.2015.00139.
4 Liu Y,Weiguang LI,Linyan HU,et al.Downregulation of nitric oxide by electroacupuncture against hypoxic-ischemic brain damage in rats via nuclear factor-κB/neuronal nitric oxide synthase[J].Mol Med Rep,2015,11(2):837.DOI:10.3892/mmr.2014.2879.
5 Nagano T.Practical methods for detection of nitric oxide[J].Luminescence,1999,14(6):283-290.DOI:10.1002/(SI-CI)1522-7243(199911/12)14:6<283:AID-BIO572>3.0.CO;2-G.
6 Eroglu E,Rost R,Bischof H,et al.Application of genetically encoded fluorescent nitric oxide(NO·)probes,the ge NOps,for real-time imaging of NO·signals in single cells[J].J Vis Exp,2017(121).DOI:10.3791/55486.
7 Monzon CM,Occhipinti R,Pignataro OP,et al.Nitric oxide reduces paracellular resistance in rat thick ascending limbs by increasing Na+and Cl-permeabilities[J].Am J Physiol Renal Physiol,2017,312(6):F1035.DOI:10.1152/ajprenal.00671.2016.
8 Keyser BM,Andres DK,Holmes WW,et al.Mustard gas inhalation injury:therapeutic strategy[J].Int J Toxicol,2014,33(4):271.DOI:10.1177/1091581814532959.
9李守林,陈维秀,陈雨历.神经源性膀胱组织中一氧化氮合酶表达的特点和意义[J].中华泌尿外科杂志,2010,31(10):695-699.DOI:10.3760/cma.j.issn.1000-6702.2010.10.014.Li SL,Chen WX,Chen YL.Biological significance of expression of nitric oxide synthases in neurogenic bladder[J].Chin J Urol,2010,31(10):695-699.DOI:10.3760/cma.j.issn.1000-6702.2010.10.014.
10李守林,刘晓东,姜俊海,等.血浆和尿中内皮素、一氧化氮在神经源性膀胱中的检测及意义[J].临床小儿外科杂志,2014(6):488-491.DOI:10.3969/j.issn.1671-6353.2014.06.005.Li SL,Liu XD,Jiang JH.The clinical significance of endothelin and nitrogen monoxidum of plasma and urinary in neurogenic bladder diseases[J].J Clin Ped Sur,2014(6):488-491.DOI:10.3969/j.issn.1671-6353.2014.06.005.
11 Austin PF,Casale AJ,Cain MP,et a1.Lipopolysaccharide and inflammatory cytokines cause an inducible nitric oxide synthase-dependent bladder smooth muscle librotic response[J].J Utol,2003,170:645-648.
12 Graziottin A,Gambini D.Anatomy and physiology of genital organs-women[J].Handb Clin Neurol,2015,130(4):39.DOI:10.1016/B978-0-444-63247-0.00004-3.
13 Fernandes VS,Ribeiro AS,Martínez-Sáenz A,et al.Underlying mechanisms involved in progesterone-induced relaxation to the pig bladder neck[J].Eur J Pharmacol,2014,723(1):246-252.
14 Lieb J,Kogan B,Das AK,et al.The effect of urine volume and nitric oxide on basal bladder blood flow:response to catheterization and drainage[J].Neurourol Urodyn,2015,20(1):115-124.
15 Govorov A,Kasyan G,Priymak D,et al.Tadalafil in the management of lower urinary tract symptoms:a review of the literature and current practices in Russia[J].Cent European J Urol,2014,67(2):167.DOI:10.5173/ceju.2014.02.art10.
16 Reitz A,Bretscher S,Knapp PA,et al.The effect of nitric oxide on the resting tone and the contractile behaviour of the external urethral sphincter:a functional urodynamic study in healthy humans[J].Eur Urol,2004,45(3):367-73.DOI:10.1016/j.eururo.2003.10.002.
17 Fernandes VS,Hernández M.The role of nitric oxide and hydrogen sulfide in urinary tract function[J].Basic Clin Pharmacol Toxicol,2016,119(S3):34-41.DOI:10.1111/bcpt.12565.
18范应中,王家祥,杨险峰.反射亢进型神经源性尿失禁膀胱逼尿肌中氮能神经及一氧化氮的变化对膀胱功能的影响[J].中国组织工程研究,2004,8(14):2694-2695.DOI:10.3321/j.issn:1673-8225.2004.14.067.Fan YZ,Wang JX,Yang XF.Influence of changes of nitric oxide synthase-immunoreactive nerve and level of nitric oxide in detrusor of bladder of huperreflexic neuropathic urinary incontinence on bladder function[J].J Chin Tis Eng Res,2004,8(14):2694-2695.DOI:10.3321/j.issn:1673-8225.2004.14.067.
19 Rocha JN.Effect of S-methyl-l-thiocitrulline dihydrochloride on rat micturition reflex[J].Int Braz J Urol,2016,42(5):1018-1027.DOI:10.1590/S1677-5538.IBJU.2015.0153.
20 Kakizaki H,Fraser MO,De Groat WC.Reflex pathways controlling urethral striated and smooth muscle function in the male rat[J].Am J Physiol,1997,272(5 Pt 2):R1647.
21 Moro C,Leeds C,Chess-Williams R.Contractile activity of the bladder urothelium/lamina propria and its regulation by nitric oxide[J].Eur J Pharmacol,2012,674(2-3):445-449.DOI:10.1016/j.ejphar.2011.11.020.
22 Johansson R,Andersson KE,Persson K.Nerve-mediated bladder contraction is impaired by cytokines:involvement of inducible nitric oxide synthase[J].Eur J Pharmacol,2003,476(3):221-227.DOI:0.1016/S0014-2999(03)02178-2.
23 Pereira ML,D'Ancona CA,Rojas-Moscoso JA,et al.Effects of nitric oxide inhibitors in mice with bladder outlet obstruction[J].Int Braz J Urol,2017,43(2):356-366.DOI:10.1590/S1677-5538.IBJU.2015.0441.
24 Birder LA,Wolf-Johnston A,Buffington CA,et al.Altered inducible nitric oxide synthase expression and nitric oxide production in the bladder of cats with feline interstitial cystitis[J].J Urol,2005,173(2):625-629.DOI:10.1097/01.ju.0000145900.22849.1d
25 Lemack GE,Zimmern PE,Vazquez D,et al.Altered response to partial bladder outlet obstruction in mice lacking inducible nitric oxide synthase[J].J Urol,2000,163(6):1981-1987.DOI:10.1097/00005392-200006000-00106.
26 Whitbeck C,Chichester P,Sokol R,et al.Role of nitric oxide in urinary bladder function:effect of L-arginine[J].Urol Int,2007,78(1):30-36.DOI:10.1159/000096931.
27 Miyaoka R,Mendes C,Schenka A,et al.BAY 41-2272,a soluble guanylate cyclase stimulator,relaxes isolated human ureter in a standardized in vitro model[J].Urology,2014,83(1):256.e1-256.e7.DOI:10.1016/j.urology.2013.09.005.
28 Ozen G,Gomez I,Daci A,et al.m PGES-1 inhibitor reduces human vascular tone by increasing PGI 2:a safer alternative to COXIBs:m PGES-1 inhibitor reduces vasoreactivity[J].Br J Pharmacol,2017.DOI:10.1111/bph.13939.
29 Salerno L,Sorrenti V,Di GC,et al.Progress in the development of selective nitric oxide synthase(NOS)inhibitors[J].Curr Pharm Des,2002,8(3):177-200.
30 Wang J,Hao L,Wang Y,et al.Inhibition of poly(ADP-ribose)polymerase and inducible nitric oxide synthase protects against ischemic myocardial damage by reduction of apoptosis[J].Mol Med Rep,2015,11(3):1768-1776.DOI:10.3892/mmr.2014.2977.
31 Bortolanza M,Cavalcantikiwiatkoski R,Padovanneto FE,et al.Glial activation is associated with l-DOPA induced dyskinesia and blocked by a nitric oxide synthase inhibitor in a rat model of Parkinson's disease[J].Neurobiol Dis,2015,73:377-387.DOI:10.1016/j.nbd.2014.10.017.
32 Broom L,Marinovamutafchieva L,Sadeghian M,et al.Neuroprotection by the selective i NOS inhibitor GW274150 in a model of Parkinson disease[J].Free Radic Biol Med,2011,50(5):633.DOI:10.1016/j.freeradbiomed.2010.12.026.
33 Mertas A,Duliban H,Szliszka E,et al.N-[3-(aminomethyl)benzyl]acetamidine(1400 W)as a potential immunomodulatory agent[J].Oxid Med Cell Longev,2014,2014(7):491214-491214.DOI:10.1155/2014/491214.
34 Nemec A,Pavlica Z,Petelin M,et al.Systemic use of selective i NOS inhibitor 1400W or non-selective NOS inhibitor lNAME differently affects systemic nitric oxide formation after oral porphyromonas gingivalis inoculation in mice[J].Arch Oral Biol,2010,55(7):509-514.DOI:10.1016/j.archoralbio.2010.04.003.
35 Garcin ED,Arvai AS,Rosenfeld RJ,et al.Anchored plasticity opens doors for selective inhibitor design in nitric oxide synthase[J].Nat Chem Biol,2008,4(11):700-7.DOI:10.1038/nchembio.115.
36 Yuji Tajiri,Valdemar Grill.Aminoguanidine exerts aβ-cell function-preserving effect in high glucose-culturedβ-cells(INS-1)[J].Int J Exp Diabetes Res,2015,1(2):111-119.
37 Lafioniatis A,Orfanidou MA,Papadopoulou ES,et al.Effects of the inducible nitric oxide synthase inhibitor aminoguanidine in two different rat models of schizophrenia[J].Behav Brain Res,2016,309:14-21.DOI:10.1016/j.bbr.2016.04.043.
38 Labuda CJ,Koblish M,Tuthill P,et al.Antinociceptive activity of the selective i NOS inhibitor AR-C102222 in rodent models of inflammatory,neuropathic and post-operative pain[J].Eur J Pain,2006,10(6):505-512.DOI:10.1016/j.ejpain.2005.07.004.
39 David D.Davey,Marc Adler,Damian Arnaiz,et al.Design,synthesis and activity of 2-imidazol-1-ylpyrimidine derived inducible nitric oxide synthase dimerization inhibitors[J].JMed Chem,2007,50(6):1146.DOI:10.1021/jm061319i.
40 Hangeland J,Friends TJ,Rossi KA,et al.Phenylimidazoles as potent and selective inhibitors of coagulation factor XIa with in vivo antithrombotic activity[J].J Med Chem,2014,57(23):9915-32.DOI:10.1021/jm5010607.