烙灸对兔膝骨性关节炎软骨细胞外基质Ⅱ型胶原、蛋白多糖表达的影响
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摘要
目的:观察烙灸疗法对兔膝骨性关节炎(KOA)软骨细胞外基质Ⅱ型胶原、蛋白多糖蛋白表达的影响。方法:选取成年健康新西兰兔30只随机分为正常对照组(10只)、造模组(20只)。采用Hulth法复制兔膝骨性关节炎模型后随机分为模型对照组(10只)和烙灸干预组(10只)。烙灸干预组给以兔左后膝关节局部烙灸治疗,1次/天,20 min/次,连续4周。疗程结束后,取动物标本进行大体形态学和HE染色病理学检测,采用蛋白免疫印迹法检测软骨Ⅱ型胶原和蛋白多糖。结果:烙灸干预组软骨组织的改良Mankin's评分、软骨外基质的Ⅱ型胶原和蛋白多糖的蛋白表达均较模型对照组降低(P <0. 05)。结论:烙灸疗法可以抑制软骨细胞外基质Ⅱ型胶原和蛋白多糖降解,改善外基质环境,减少兔KOA关节软骨的破坏。
Objective: To observe the effect of cauterization moxibustion on the expressions of collagen type Ⅱ and proteoglycan of cartilaginous extracellular matrix in rabbits with knee osteoarthritis( KOA). Methods: 30 adult healthy New Zealand rabbits were randomly divided into the blank control group( n = 10),the model group( n = 10),and the cauterization moxibustion group( n = 10). KOA model was established by Hulth method.The cauterization moxibustion was intervened with local cauterization moxibustion on the left posterior knee joint. The cauterization was once per day,20 min per time for four weeks. At the end of the treatment,the gross morphology and HE staining pathology of the animal specimens were detected,and the collagen type Ⅱ and proteoglycan of cartilage were detected by Western blot method. Results: The modified Mankin's score of cartilage tissue,the expressions of collagen Ⅱ and proteoglycan were significantly decreased in the cauterization group than those in the model group( P < 0. 05). Conclusion: Cauterization moxibustion can inhibit the degradation of collagen type Ⅱ and proteoglycan,it can improve the environment of extracellular matrix and reduce the destruction of articular cartilage in KOA rabbits.
Objective: To observe the effect of cauterization moxibustion on the expressions of collagen type Ⅱ and proteoglycan of cartilaginous extracellular matrix in rabbits with knee osteoarthritis( KOA). Methods: 30 adult healthy New Zealand rabbits were randomly divided into the blank control group( n = 10),the model group( n = 10),and the cauterization moxibustion group( n = 10). KOA model was established by Hulth method.The cauterization moxibustion was intervened with local cauterization moxibustion on the left posterior knee joint. The cauterization was once per day,20 min per time for four weeks. At the end of the treatment,the gross morphology and HE staining pathology of the animal specimens were detected,and the collagen type Ⅱ and proteoglycan of cartilage were detected by Western blot method. Results: The modified Mankin's score of cartilage tissue,the expressions of collagen Ⅱ and proteoglycan were significantly decreased in the cauterization group than those in the model group( P < 0. 05). Conclusion: Cauterization moxibustion can inhibit the degradation of collagen type Ⅱ and proteoglycan,it can improve the environment of extracellular matrix and reduce the destruction of articular cartilage in KOA rabbits.
引文
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[16]Dejica VM,Mort JS,Laverty S,et al.Increased type II collagen cleavage by cathepsin K and collagenase activities with aging and osteoarthritis in human articular cartilage[J].Arthritis Res Ther,2012,14(3):R113.
[17]魏丽杰,王文雅.炎症引起的软骨细胞外基质的变化对骨关节炎病理进展的作用[J].河北联合大学学报(医学版),2014,16(2):170-171.
[18]Lahm A,Mrosek E,Spank H,et al.Changes in content and synthesis of collagen types and proteoglycans in osteoarthritis of the knee joint and comparison of quantitative analysis with photoshop-based image analysis[J].Arch Orthop Trauma Surg,2010,130(4):557-564.
[19]童文文,李甲,徐卫东.蛋白聚糖诱导小鼠关节炎模型的研究进展[J].第二军医大学学报,2015,36(5):530-535.
[20]Hulth A,Lindbeng L,Telhag H.Experimental osteoarthritis in rabbits preliminary report[J].Acta Orthop Semld,1970,41(5):522-530.
[2]赵昌盛,钟群杰,林剑浩.中国膝关节骨关节炎流行病学调查现状[J].广东医学,2016,37(13):2050-2052.
[3]Glyn-Jones S,Palmer AJ,Agricola R,et al.Osteoarthritis[J].Lancet,2015,386(9991):376-387.
[4]Musumeci G,Loreto C,Carnazza ML,et al.Characterization of apoptosis in articular cartilage derived from the knee joints of patients with osteoarthritis[J].Knee Surg Sports Traumatol Arthrosc,2011,19(2):307-313.
[5]邵翔,陈后煌,马玉环,等.电针对骨关节炎软骨细胞外基质调节机制探讨[J].风湿病与关节炎,2015,4(7):33-36.
[6]刘发元,党传鹏,翁霞萍,等.内质网应激调控骨关节炎软骨细胞凋亡的机制[J].风湿病与关节炎,2014,3(11):55-58.
[7]徐守宇,张丽梅,姚新苗,等.低强度脉冲超声对兔膝骨关节炎软骨细胞外基质的影响及机制[J].中国骨伤,2014,27(9):766-771.
[8]许建峰,林瑞珠,牛子瞻,等.回医烙灸疗法对退变腰椎间盘中基质MMP-3表达的影响[J].福建中医药,2013,44(2):53-54.
[9]朱宁,许建峰,马奇,等.烙灸对激素性股骨头坏死骨细胞凋亡与Bcl-2、caspase-3表达影响的实验研究[J].山西中医学院学报,2018,19(2):16-19.
[10]林瑞珠,孙改玲,朱宁,等.回医烙灸对兔膝骨关节炎软骨细胞Bcl-2和Bax表达的影响[J].长春中医药大学学报,2017,33(1):34-36.
[11]David S,Gregory J,Brown A,et al.The American Academy of Orthopaedic Surgeons Evidence-Based Guideline on:Treatment of Osteoarthritis of the Knee,2nd Edition[J].The Journal of Bone and Joint Surgery,2013,95(20):1885-1886.
[12]林瑞珠,朱宁,许建峰,等.回医烙灸疗法对激素性股骨头缺血性坏死家兔血流变、血脂及骨形态学的影响[J].甘肃中医药大学学报,2018,35(1):14-17.
[13]顾庆陟,陶开亮,高兴,等.膝关节软骨下骨不同表面积缺损对关节软骨组织形态的影响[J].中华实用诊断与治疗杂志,2018,32(3):222-225.
[14]高明霞,程凯,林强,等.低强度脉冲超声波对早中期兔膝骨性关节炎软骨细胞外基质及MAPKs信号通路的影响[J].中国康复医学杂志,2013,28(7):593-599.
[15]席小芳,李学智,刘菲,等.短刺加电针对膝骨关节炎模型兔软骨细胞外基质的修复作用研究[J].中国针灸,2016,36(12):1288-1294.
[16]Dejica VM,Mort JS,Laverty S,et al.Increased type II collagen cleavage by cathepsin K and collagenase activities with aging and osteoarthritis in human articular cartilage[J].Arthritis Res Ther,2012,14(3):R113.
[17]魏丽杰,王文雅.炎症引起的软骨细胞外基质的变化对骨关节炎病理进展的作用[J].河北联合大学学报(医学版),2014,16(2):170-171.
[18]Lahm A,Mrosek E,Spank H,et al.Changes in content and synthesis of collagen types and proteoglycans in osteoarthritis of the knee joint and comparison of quantitative analysis with photoshop-based image analysis[J].Arch Orthop Trauma Surg,2010,130(4):557-564.
[19]童文文,李甲,徐卫东.蛋白聚糖诱导小鼠关节炎模型的研究进展[J].第二军医大学学报,2015,36(5):530-535.
[20]Hulth A,Lindbeng L,Telhag H.Experimental osteoarthritis in rabbits preliminary report[J].Acta Orthop Semld,1970,41(5):522-530.