摘要
实验旨在研究添加酪酸菌(Clostridium butyricum)对宝石鲈(Scortum barcoo)生长发育及肠道菌群的影响。实验选用规格一致,初始体质量为(24.02±2.04)g的宝石鲈,随机分成4个组,每组1 200尾,分别饲喂添加0(Control组)和0.1%(Diet 1组)、0.5%(Diet 2组)、1.0%(Diet 3组)酪酸菌的日粮,饲养周期为60 d。实验结果表明:(1)与Control组对比,Diet 2组宝石鲈的终体质量、相对增长率显著提高(P<0.05)。(2)随着养殖时间的增加,宝石鲈肠道菌群的物种组成更加多样化。(3)Diet组中以鲸杆菌属(Cetobacterium)、芽孢杆菌属(Bacillus)、大洋芽孢杆菌属(Oceanobacillus)为主的益生菌群的比例提高,以邻单胞菌属(Plesiomonas)、拟杆菌属(Bacteroides)、类芽孢杆菌属(Paenibacillus)为主的致病菌的含量降低。
The purpose of this study was to explore the effect of Clostridium butyricum on the growthperformance and intestinal microbiota of Scortum barcoo. The same specification of Scortum barcoowith initial weight of(24.02±2.04) g were selected for test. The test included four groups with 1 200 fishes were employed in one group. A basal diet was supplemented with Clostridium butyricum at0.0%, 0.1%, 0.5% and 1.0% feed, these four kinds of feed were named Control,Diet 1, Diet 2 and Di-et 3, respectively. And the test period was 60 days. The results showed that:(1) the final weight andthe relative growth rate of Diet 2 group was significantly higher than that of Control group(P<0.05).(2)With the increase of culture time, the species composition of intestinal microbiota of Scortum barcoobecame more diversified.(3) In the Diet group, the proportion of probiotics mainly consisting of Ceto-bacterium, Bacillus, and Oceanobacillus increased, and the content of pathogenic bacteria mainly com-posed of Plesiomonas, Bacteroides and Paenibacillus decreased.
引文
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