蒙药肋柱花HPLC指纹图谱的研究
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摘要
目的采用HPLC对15批不同采集地的蒙药肋柱花药材进行指纹图谱研究,并采用相似度评价蒙药肋柱花药材的质量。方法采用HPLC进行测定,色谱柱为YMC C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.4%磷酸水-甲醇,梯度洗脱,体积流量0.8 m L/min,检测波长254 nm,柱温30℃。运用色谱软件对指纹图谱进行相似度评价。结果建立了蒙药肋柱花药材的HPLC指纹图谱,确定了15个共有峰,指认了5个共有峰,15批次肋柱花药材与生成对照指纹图谱的相似度在0.881~0.997。结论首次建立肋柱花药材的HPLC特征指纹图谱,所建立的指纹图谱测定方法精密度、稳定性和重复性良好。
Objective To study the fingerprints of 15 batches of Mongolian medicinal materials of Lomatogonium rotatum collected from different places by HPLC, and to evaluate the quality of the medicinal materials of L. rotatum by similarity calculation. Methods A total of 15 batches of ribbed flowers were collected by HPLC. Chromatographic column: YMC C18 column(250 mm × 4.6 mm, 5 μm), mobile phase: acetonitrile-0.4% phosphoric acid-methanol, gradient elution, flow rate was 0.8 m L/min, detection wavelength was 254 nm, column temperature was 30 ℃. The similarity of fingerprints was evaluated by using the "Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine 2004 A Edition". Results The fingerprints of L. rotatum of Mongolian medicine were established, 15 common peaks were identified, five common peaks were identified, and the similarity among 15 batches of L. rotatum and the fingerprints of control was in the range of 0.881—0.997. The fingerprints of erect column flowers have good precision, stability and reproducibility. Conclusion The characteristic fingerprint of L. rotatum was established for the first time, which not only provides a new scientific basis for the identification and quality control of L. rotatum, but also has important significance for the quality evaluation of L. rotatum.
Objective To study the fingerprints of 15 batches of Mongolian medicinal materials of Lomatogonium rotatum collected from different places by HPLC, and to evaluate the quality of the medicinal materials of L. rotatum by similarity calculation. Methods A total of 15 batches of ribbed flowers were collected by HPLC. Chromatographic column: YMC C18 column(250 mm × 4.6 mm, 5 μm), mobile phase: acetonitrile-0.4% phosphoric acid-methanol, gradient elution, flow rate was 0.8 m L/min, detection wavelength was 254 nm, column temperature was 30 ℃. The similarity of fingerprints was evaluated by using the "Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine 2004 A Edition". Results The fingerprints of L. rotatum of Mongolian medicine were established, 15 common peaks were identified, five common peaks were identified, and the similarity among 15 batches of L. rotatum and the fingerprints of control was in the range of 0.881—0.997. The fingerprints of erect column flowers have good precision, stability and reproducibility. Conclusion The characteristic fingerprint of L. rotatum was established for the first time, which not only provides a new scientific basis for the identification and quality control of L. rotatum, but also has important significance for the quality evaluation of L. rotatum.
引文
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[9]道日娜.蒙药地格达属的指纹图谱比较研究[J].中国民族医药杂志,2016,22(12):56-58.
[10]李玉林,丁晨旭,王洪伦,等.辐状肋柱花的苷类成分[J].西北植物学报,2006(1):197-200.
[11]贾凌云,李倩,袁久志,等.蒙药肋柱花化学成分的分离与鉴定[J].沈阳药科大学学报,2010,27(9):704-706.
[2]中华人民共和国卫生部药品标准(蒙药分册)[S].1998.
[3]内蒙古蒙药材标准[S].1987.
[4]赵一之.内蒙古肋柱花属植物分类及其地理分布研究[J].植物研究,2004(1):7-8.
[5]叶日贵,白梅荣,包明兰,等.蒙药肋柱花化学成分的研究[J].北方药学,2014,11(12):20-21.
[6]贾凌云,袁久志,孙启时.蒙药肋柱花化学成分的分离与鉴定(2)[J].沈阳药科大学学报,2011,28(4):260-262.
[7]李振华.内蒙古地区蒙药肋柱花生态适宜性区划及野生抚育初步研究[D].包头:内蒙古科技大学包头医学院,2015.
[8]田峦鸢.紫红獐牙菜化学成分和HPLC指纹图谱的研究[D].武汉:湖北中医学院,2006.
[9]道日娜.蒙药地格达属的指纹图谱比较研究[J].中国民族医药杂志,2016,22(12):56-58.
[10]李玉林,丁晨旭,王洪伦,等.辐状肋柱花的苷类成分[J].西北植物学报,2006(1):197-200.
[11]贾凌云,李倩,袁久志,等.蒙药肋柱花化学成分的分离与鉴定[J].沈阳药科大学学报,2010,27(9):704-706.