基于灰色关联的苗药红禾麻总黄酮含量影响因子分析
|
摘要
采用Al Cl3比色法对苗药红禾麻42个居群中的总黄酮含量进行分析测定,并用土壤养分测定仪测定对应产地土壤中的铵态氮、速效磷、有效钾、p H值和水分,通过向距产地较近的气象部门查询和以全球卫星定位系统GPS、海拔表等测定地理气候因子,结合42个居群红禾麻ISSR遗传多样性分析结果,运用灰色关联度分析法对红禾麻不同种质资源药材中总黄酮的含量与各影响因子进行相关性分析。结果表明:42个红禾麻不同种质资源药材总黄酮含量为0.42%~2.16%,平均加样回收率为97.86%,RSD为1.5%;环境因子和遗传因子中与总黄酮含量关联度较大的因素分别为无霜期和Shannon信息指数I,而各影响因子与总黄酮含量的关联度中,以Shannon信息指数I(r=1.03)最大,土壤p H(r=0.49)最小,表明遗传因子对红禾麻药材总黄酮含量的影响大于环境因子。该研究结果为红禾麻药材优良种质资源筛选及野生变家种研究提供了依据。
The total flavonoids content of 42 groups of Guizhou miao medicine Laportea bulbifera were analyzed and determinated by Al Cl3 colorimetric. The ammonium nitrogen,available phosphorus,available potassium,p H and water were determinated in the soil by soil nutrients tester and the origin of geographical climatic factors of L. bulbifera from different producing areas were consulted. In order to analyze the total flavonoids contents of different germplasm resources and impact factors by grey correlation degree analysis,geographical climate factors were determinated by the closed meteorogical department who had been inquired data and GPS,combined with the ISSR results of genetic diversity of 42 groups with the above measured data. The result revealed that the flavonoid contents of L. bulbifera in 42 groups of different germplasm resources was at 0. 42%-2. 16%,the average recovery rate was 97. 86% and RSD was 1. 5%,respectively. Frostfree period and the Shannon information index I were the biggest factors with the flavonoid contents in the environment factors and genetic factors. And the biggest correlation degree was Shannon information index I( r = 1.03)and the smallest was the soil p H( r = 0.49) in all of correlation degrees. It could be judged that genetic factor had more influence on the quality of medicinal materials L. bulbifera than environmental factor. The results provide the reference for choosing good germplasm resources and change the wild to the domestic.
The total flavonoids content of 42 groups of Guizhou miao medicine Laportea bulbifera were analyzed and determinated by Al Cl3 colorimetric. The ammonium nitrogen,available phosphorus,available potassium,p H and water were determinated in the soil by soil nutrients tester and the origin of geographical climatic factors of L. bulbifera from different producing areas were consulted. In order to analyze the total flavonoids contents of different germplasm resources and impact factors by grey correlation degree analysis,geographical climate factors were determinated by the closed meteorogical department who had been inquired data and GPS,combined with the ISSR results of genetic diversity of 42 groups with the above measured data. The result revealed that the flavonoid contents of L. bulbifera in 42 groups of different germplasm resources was at 0. 42%-2. 16%,the average recovery rate was 97. 86% and RSD was 1. 5%,respectively. Frostfree period and the Shannon information index I were the biggest factors with the flavonoid contents in the environment factors and genetic factors. And the biggest correlation degree was Shannon information index I( r = 1.03)and the smallest was the soil p H( r = 0.49) in all of correlation degrees. It could be judged that genetic factor had more influence on the quality of medicinal materials L. bulbifera than environmental factor. The results provide the reference for choosing good germplasm resources and change the wild to the domestic.
引文
Chinese Pharmacopoeia Commission,2015.Chinese pharmacopoeia:the fourth part[M].Beijing:China Medical Science Press:37-40,103-105.[国家药典委员会,2015.《中国药典》第四部[M].北京:中国医药科技出版社:37-40,103-105.]
CAI P,LIU CY,LIANG XJ,et al,2014.Grey correlation degree analysis between active ingredients in barks or leaves of Eucommiae cortex and environmental factors in their habitats[J].Chin J Exp Trad Med Form,20(23):10-14.[蔡萍,刘才英,梁雪娟,等,2014.杜仲药材有效成分与环境因子的灰色关联度分析[J].中国实验方剂学杂志,20(23):10-14.]
Food and Drug Administration of Guizhou Province,2003.Quality standard of Chinese medicinal materials and national medicines in Guizhou Province[S].Guiyang:Guizhou Science and Technology Publishing House Co.,Ltd:187.[贵州省食品药品监督管理局.2003.贵州省中药材、民族药材质量标准[S].贵阳:贵州科技出版社:187.]
HOU WR,SU ZQ,PI HF,et al,2010.Immunosuppressive constituents from Urtica dentata Hand[J].JANPR,12(8):707-713.[侯文睿,苏志强,皮慧芳,等,2010.红活麻免疫抑制活性成分研究[J].亚洲天然产物研究杂志,12(8):707-713.]
LI Q,UANG ZS,DONG JE,et al,2010.Grey correlation for main climatic factors and quality of Danshen(Salvia miltiorrhiza Bunge[J].Acta Ecol Sin,30(10):2569-2575.[李倩,梁宗锁,董娟娥,等,2010.丹参品质与主导气候因子的灰色关联度分析[J].生态学报,30(10):2569-2575.]
LUO Y,SUN QW,2013.National common natural medicine in Guizhou:Vol.2[M].Guiyang:Guizhou Science and Technology Publishing House Co.,Ltd:231-232.[罗迎春,孙庆文,2013.贵州民族常用天然药物:第2卷[M].贵阳:贵州科技出版社:231-232.]
MINCHEOL YANG,SANG ZIN CHOI,OK LEE,et al,2003.Flavonoid constituents and their antioxidant activity of Laportea bulbifera Weddell[J].Kor J Pharmacogn,34(1):18-24.
MA L,LIANG B,ZHU Z,et al,2012.Effects of analgesic pharmacological of national medicine Laportea bulbifera Weddell[J].J Guiyang Coll Trad Chin Med 3(1):26-26.[马琳,梁冰,朱珠,等,2012.民族药珠芽艾麻醇提物镇痛药理作用的研究[J].贵阳中医学院学报,3(1):26-26.]
State Administration of Traditional Chinese Medicine,the Chinese Material Medical the Editorial Committee,2005.The Chinese material medical(Miao medicine volume)[M].Guiyang:Guizhou Science and Technology Publishing House Co.,Ltd:283-284.[国家中医药管理局,《中华本草》编委会.2005.中华本草(苗药卷)[M].贵阳:贵州科技出版社:283-284.]
SU ZQ,ZHAO ZY,XIE SN,et al,2009.Effects of analgesic antiinflammation and immune suppression of acetic ether extract of Chinese medicine honghuoma[J].Chin Pharmacol Bull,25(4):559-560.[苏志强,赵增宇,谢胜男,等,2009.红禾麻提取物镇痛抗炎和免疫抑制活性研究[J].中国药理学通报,25(4):559-560.]
WANG ZQ,JIA JM,Song J,2010.Analysis of grey correlation between saponins and environmental factors in Astragali Radix[J].Chin Trad Herb Drugs,41(10):1709-1712.[王宗权,贾继明,宋剑,2010.黄芪皂苷成分与环境因子的灰色关联度分析[J].中草药,41(10):1709-1712.]
XIANG M,HOU WR,XIE SN,et al,2009.Immunosuppressive effects of an ethyl acetate extract from Urtica dentata Hand on skin allograft rejection[J].J Ethnopharmacol,126(1):57-63.[向明,侯文睿,谢胜男,等,2009.基于同种移植皮片排异反应的红活麻乙酸乙酯提取物免疫抑制活性研究[J].民族药理学,126(1):57-63.]
XIANG M,TAO E,CHU T,et al,2006.Preliminary study on preventing effects of the active component of Honghuoma on CIA modie[J].Chin Hosp Pharm J,26(10):1201-1205.[向明,陶恩,储潼,等,2006.红活麻有效部位干预Ⅱ型胶原所致关节炎的作用[J].中国医院药学杂志,26(10):1201-1205.]
CAI P,LIU CY,LIANG XJ,et al,2014.Grey correlation degree analysis between active ingredients in barks or leaves of Eucommiae cortex and environmental factors in their habitats[J].Chin J Exp Trad Med Form,20(23):10-14.[蔡萍,刘才英,梁雪娟,等,2014.杜仲药材有效成分与环境因子的灰色关联度分析[J].中国实验方剂学杂志,20(23):10-14.]
Food and Drug Administration of Guizhou Province,2003.Quality standard of Chinese medicinal materials and national medicines in Guizhou Province[S].Guiyang:Guizhou Science and Technology Publishing House Co.,Ltd:187.[贵州省食品药品监督管理局.2003.贵州省中药材、民族药材质量标准[S].贵阳:贵州科技出版社:187.]
HOU WR,SU ZQ,PI HF,et al,2010.Immunosuppressive constituents from Urtica dentata Hand[J].JANPR,12(8):707-713.[侯文睿,苏志强,皮慧芳,等,2010.红活麻免疫抑制活性成分研究[J].亚洲天然产物研究杂志,12(8):707-713.]
LI Q,UANG ZS,DONG JE,et al,2010.Grey correlation for main climatic factors and quality of Danshen(Salvia miltiorrhiza Bunge[J].Acta Ecol Sin,30(10):2569-2575.[李倩,梁宗锁,董娟娥,等,2010.丹参品质与主导气候因子的灰色关联度分析[J].生态学报,30(10):2569-2575.]
LUO Y,SUN QW,2013.National common natural medicine in Guizhou:Vol.2[M].Guiyang:Guizhou Science and Technology Publishing House Co.,Ltd:231-232.[罗迎春,孙庆文,2013.贵州民族常用天然药物:第2卷[M].贵阳:贵州科技出版社:231-232.]
MINCHEOL YANG,SANG ZIN CHOI,OK LEE,et al,2003.Flavonoid constituents and their antioxidant activity of Laportea bulbifera Weddell[J].Kor J Pharmacogn,34(1):18-24.
MA L,LIANG B,ZHU Z,et al,2012.Effects of analgesic pharmacological of national medicine Laportea bulbifera Weddell[J].J Guiyang Coll Trad Chin Med 3(1):26-26.[马琳,梁冰,朱珠,等,2012.民族药珠芽艾麻醇提物镇痛药理作用的研究[J].贵阳中医学院学报,3(1):26-26.]
State Administration of Traditional Chinese Medicine,the Chinese Material Medical the Editorial Committee,2005.The Chinese material medical(Miao medicine volume)[M].Guiyang:Guizhou Science and Technology Publishing House Co.,Ltd:283-284.[国家中医药管理局,《中华本草》编委会.2005.中华本草(苗药卷)[M].贵阳:贵州科技出版社:283-284.]
SU ZQ,ZHAO ZY,XIE SN,et al,2009.Effects of analgesic antiinflammation and immune suppression of acetic ether extract of Chinese medicine honghuoma[J].Chin Pharmacol Bull,25(4):559-560.[苏志强,赵增宇,谢胜男,等,2009.红禾麻提取物镇痛抗炎和免疫抑制活性研究[J].中国药理学通报,25(4):559-560.]
WANG ZQ,JIA JM,Song J,2010.Analysis of grey correlation between saponins and environmental factors in Astragali Radix[J].Chin Trad Herb Drugs,41(10):1709-1712.[王宗权,贾继明,宋剑,2010.黄芪皂苷成分与环境因子的灰色关联度分析[J].中草药,41(10):1709-1712.]
XIANG M,HOU WR,XIE SN,et al,2009.Immunosuppressive effects of an ethyl acetate extract from Urtica dentata Hand on skin allograft rejection[J].J Ethnopharmacol,126(1):57-63.[向明,侯文睿,谢胜男,等,2009.基于同种移植皮片排异反应的红活麻乙酸乙酯提取物免疫抑制活性研究[J].民族药理学,126(1):57-63.]
XIANG M,TAO E,CHU T,et al,2006.Preliminary study on preventing effects of the active component of Honghuoma on CIA modie[J].Chin Hosp Pharm J,26(10):1201-1205.[向明,陶恩,储潼,等,2006.红活麻有效部位干预Ⅱ型胶原所致关节炎的作用[J].中国医院药学杂志,26(10):1201-1205.]