宽叶打碗花化学成分研究
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摘要
目的研究宽叶打碗花Calystegia sepium中的化学成分。方法利用硅胶柱色谱、凝胶柱色谱、反相柱色谱和制备液相色谱等方法进行分离纯化,通过理化性质、波谱数据并结合文献报道数据鉴定化合物结构。结果从宽叶打碗花中分离得到20个化合物,分别鉴定为酪醇(1)、槲皮素-3-O-β-D-吡喃葡糖苷(2)、野黄芩素-7-O-β-吡喃葡萄糖苷(3)、紫云英苷(4)、山柰酚-3-O-半乳糖苷(5)、山柰酚-3-O-β-刺槐双糖苷(6)、烟花苷(7)、芦丁(8)、尿苷(9)、它乔糖苷(10)、2-甲氧基-4-(2′-羟乙基)-苯基-1-O-β-D-吡喃葡萄糖苷(11)、4-羟基苯乙醇-4-O-β-D-吡喃葡萄糖苷(12)、polybotrin(13)、吡啶-3,5-二甲酰胺(14)、茵芋苷(15)、苯甲醇-β-D-葡萄糖苷(16)、5-羟基-2-羟甲基吡啶(17)、云杉苷(18)、5-羟甲基糠醛(19)、sessiline(20)。结论所有化合物均为首次从该植物中分离得到。
Objective To study the chemical constituents from Calystegia sepium. Methods The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20, ODS column chromatography, preparative HPLC and so on. Their structures were determined on the basis of physicochemical properties and their spectroscopic data, as well as literatures. Results A total of 20 compounds were separated and identified as tyrosol(1), quercetin-3-O-β-D-glucopyranoside(2), scutellarein-7-O-β-glucopyranoside(3), kaempferol-3-O-β-D-glucopyranoside(4), kaempferol-3-O-galactoside(5), kaempferol-3-O-β-robinobioside(6), nicotiflorin(7), rutin(8), uridine(9), tachioside(10), 2-methoxy-4-(2′-hydroxyethyl)-phenyl-1-O-β-D-glucopyranoside(11), 4-hydroxyphenethylol-4-O-β-D-glucopyranoside(12), polybotrin(13), pyridine-3,5-dicarboamide(14), skimmin(15), benzylalcohol-β-D-glucopyranoside(16), 5-hydroxyl-2-hydroxymethylpyridine(17), picein(18), 5-hydroxymethyl-furaldehyde(19), and sessiline(20). Conclusion Compounds 1—20 are isolated from Calystegia sepium for the first time.
Objective To study the chemical constituents from Calystegia sepium. Methods The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20, ODS column chromatography, preparative HPLC and so on. Their structures were determined on the basis of physicochemical properties and their spectroscopic data, as well as literatures. Results A total of 20 compounds were separated and identified as tyrosol(1), quercetin-3-O-β-D-glucopyranoside(2), scutellarein-7-O-β-glucopyranoside(3), kaempferol-3-O-β-D-glucopyranoside(4), kaempferol-3-O-galactoside(5), kaempferol-3-O-β-robinobioside(6), nicotiflorin(7), rutin(8), uridine(9), tachioside(10), 2-methoxy-4-(2′-hydroxyethyl)-phenyl-1-O-β-D-glucopyranoside(11), 4-hydroxyphenethylol-4-O-β-D-glucopyranoside(12), polybotrin(13), pyridine-3,5-dicarboamide(14), skimmin(15), benzylalcohol-β-D-glucopyranoside(16), 5-hydroxyl-2-hydroxymethylpyridine(17), picein(18), 5-hydroxymethyl-furaldehyde(19), and sessiline(20). Conclusion Compounds 1—20 are isolated from Calystegia sepium for the first time.
引文
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[3]Zhou Z H,Zhang Y J,Yang C R.Saluenin,a new flavonol glycoside from Camellia saluenensis[J].Acta Botanica Yunnanica,2000,22(1):90-96.
[4]褚洪标,贺文军,张玉梅,等.密穗马先蒿中黄酮和降倍半萜成分[J].中国中药杂志,2011,36(19):2672-2675.
[5]Hari K P,Vijaya B R M,Gunasekar D,et al.A new coumestan from Tephrosia calophylla[J].Chem Pharm Bull,2003,51(2):194-196.
[6]Liu C D,Jing C,Wang J H.A novel kaempferol triglycoside from flower buds of Panax quinquefolium[J].Chem Nat Compds,2009,45(6):808-810.
[7]Yang N Y,Duan J A,Ping L I,et al.Studies on flavonoids of Lysimachia christinae Hance[J].Chin Pharm J,2006,41(21):1621-1624.
[8]王先.芳樟叶黄酮和多糖的提取分离、结构鉴定及抗氧化活性研究[D].厦门:厦门大学,2008.
[9]栾晓莹,白洋,陈颖,等.心脑舒通中黄酮类成分研究[J].天津中医药大学学报,2015,34(5):291-295.
[10]艾凤伟,张嵩,李艳凤,等.白附子的化学成分研究[J].中草药,2010,41(2):201-203.
[11]肖凯,宣利江,徐亚明,等.虎杖的化学成分研究[J].中国药学杂志,2003,38(1):12-14.
[12]成军,梁鸿,王媛,等.中药鸡血藤化学成分的研究[J].中国中药杂志,2003,28(12):1153-1155.
[13]张罗红,彭艳,都述虎.红景天苷原料药中有关物质的分离与结构鉴定[J].中药与临床,2010,1(3):21-23.
[14]Zou K,Komatsu K,Zhu S.A novel compound from Hedysarum polybotrys[J].J Asian Nat Prods Res,2007,9(8):699-703.
[15]Ma Z,Yang X,Zhong G.A new flavonoid glucoside from Huanglian jiedutang decoction[J].China J Chin Mater Med,2009,34(9):1097-1100.
[16]Chiang C Y,Leu Y L,Chan Y Y,et al.Sodium aristolochates from the flowers and fruits of Aristolochia zollingeriana[J].J Chin Chem Soc,1998,45(1):93-97.
[17]Zhao J,Ming Y,Yi H,et al.Study on chemical constituents from Juniperus sabina L.[J].Chin Pharm J,2008,43(19):1461-1463.
[18]刘莹,李喜凤,刘艾林,等.细皱香薷叶的化学成分研究[J].中草药,2009,40(9):1356-1359.
[19]段洁,李巍,胡旭佳,等.九子参化学成分研究[J].中草药,2009,40(4):528-530.
[20]Kim J W,Kim T B,Yang H,et al.Phenolic compounds isolated from Opuntia ficus-indica fruits[J].Nat Prod Sci,2016,22(2):117-121.
[21]李行诺,周孟宇,沈培强,等.生地黄化学成分研究[J].中国中药杂志,2011,36(22):3125-3129.
[22]Lee S,Ji J,Shin K H,et al.Sessiline,a new nitrogenous compound from the fruits of Acanthopanax sessiliflorus[J].Planta Med,2002,68(10):939-941.