拟丝孢镰刀菌(Fusarium trichothecioides)侵染马铃薯块茎过程中抗氧化酶活性及MDA含量变化研究（英文）
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摘要
[目的]探讨拟丝孢镰刀菌(Fusarium trichothecioides)侵染马铃薯块茎的生理生化特性。[方法]采用拟丝孢镰刀菌对大西洋和克山1的薯块进行侵染,在不同时间对其内部的可溶性蛋白质含量、SOD活性、POD活性和丙二醛含量进行测定。[结果]不同抗性的马铃薯被拟丝孢镰刀菌侵染后,薯块内可溶性蛋白和丙二醛含量含量都呈现出下降的趋势,而不断上升的SOD比活性对于薯块抵抗病原菌的侵染起到了一定的作用,抗性品种内的POD酶活性高于感病品种,表明POD酶活性同抗病性密切相关。[结论]确定病原菌的侵染特性对于早期预报和防治干腐病具有重要的意义。
[Objective]This study was conducted to determine the physiological and biochemical characteristics of potato tubers infected by Fusarium trichothecioides.[Method]The soluble protein content,SOD activity,POD activity and malondialdehyde(MDA) content of the tubers of two potato varieties Atlantic and Keshan 1 infected by F.trichothecioides were measured 0,3,6,9,12,24 and 48 h later.[Result]Compared with the control uninfected,the contents of soluble protein and MDA in the tubers of both potato varieties infected with F.trichothecioides decreased,while the SOD activity increased,indicating that SOD activity had a certain relationship with the resistance of potato to dry rot.The POD activity in Keshan 1 which is resistant to F.trichothecioides was higher than in Atlantic,which is susceptible to F.trichothecioides,indicating that POD activity was closely related with potato's resistance to Fusarium dry rot.[Conclusion]The physiological and biochemical characteristics of potato tubers infected by F.trichothecioides will provide some references for the early warning and prevention of Fusarium dry rot.
[Objective]This study was conducted to determine the physiological and biochemical characteristics of potato tubers infected by Fusarium trichothecioides.[Method]The soluble protein content,SOD activity,POD activity and malondialdehyde(MDA) content of the tubers of two potato varieties Atlantic and Keshan 1 infected by F.trichothecioides were measured 0,3,6,9,12,24 and 48 h later.[Result]Compared with the control uninfected,the contents of soluble protein and MDA in the tubers of both potato varieties infected with F.trichothecioides decreased,while the SOD activity increased,indicating that SOD activity had a certain relationship with the resistance of potato to dry rot.The POD activity in Keshan 1 which is resistant to F.trichothecioides was higher than in Atlantic,which is susceptible to F.trichothecioides,indicating that POD activity was closely related with potato's resistance to Fusarium dry rot.[Conclusion]The physiological and biochemical characteristics of potato tubers infected by F.trichothecioides will provide some references for the early warning and prevention of Fusarium dry rot.
引文
[1]XIE JH(谢建华).Potato production situation and development countermeasures in China(我国马铃薯生产现状及发展对策)[J].China Agricultural Technology Extension(中国农技推广),2007,(5):4-7.
[2]SUN DS(孙东升),LIU GH(刘合光).The analysis and prospect outlook of China's potato industry(我国马铃薯产业发展现状及前景展望)[J].Agricultural Outlook(农业展望),2009,(3):25-28.
[3]SONG BF(宋伯符).Promising potato industry in China(充满朝阳的中国马铃薯产业)[A].Potato industry and high technologies(马铃薯产业与高新技术)[C].Harbin:Harbin Engineering University Press(哈尔滨:哈尔滨工程大学出版社),2002:185-188.
[4]QU DY(屈冬玉),XIE KY(谢开云),JIN LP(金黎平),et al.Development of potato industry and food security in China(中国马铃薯产业发展与食物安全)[J].Scientia Agricultura Sinica(中国农业科学),2005,(2):258-262.
[5]TIAN F(田芳).Occurrence and control of diseases of potato in cellars in Northern China(北方地区马铃薯窖藏病害的发生及防治)[J].Guide of Sci-tech Magazine(科技致富向导),2012,(27):378-402.
[6]CARNEGIE SF,RUTHVEN AD,LINDSAY DA,et al.Effects of fungicides applied to seed potato at harvest or after grading on fungal storage diseases and plant development[J].Ann Appl Biol,1990,116:61-72.
[7]ZHAO SS(赵生山),NIU LH(牛乐华).Survey on potato disease and chemical control during storage(马铃薯贮藏期病害调查及药剂防治研究)[J].Information of Agricultural Science and Technology(农业科技与信息),2008,(11):44-46.
[8]PENG XW(彭学文),ZHU WH(朱杰华).Fungal diseases and distribution of in potato in Hebei Province(河北省马铃薯真菌病害种类及分布)[J].Chinese Potato Journal(中国马铃薯),2008,22(1):31-33.
[9]LIU CH(刘程惠),HU WZ(胡文忠).Physio-biochemical changes of freshcut potato at different storage temperatures(不同贮藏温度下鲜切马铃薯的生理生化变化)[J].Food&Machinery(食品与机械),2008,24(2):38-42.
[10]LI GL(李国龙),WU HX(吴海霞),WEN L(温丽).Progress in physiological and molecular mechanism of drought resistant in crop(作物抗旱生理与分子作用机制研究进展)[J].Chinese Agricultural Science Bulletin(中国农学通报),2010,40(23):185-191.
[11]CHEN Q,SUN HS,YANG JL,et al.Inheritance and molecular mapping of new genes conferring late blight and CPB resistance in Mexican wild potato species Solanum pinnatisectum[J].Agricultural Science&Technology,2009(5):257-261.
[12]ZHANG RJ,CHEN YJ,MENG Ml,et al.Regulation of nitrogen on potato under Na Cl stress[J].Agricultural Science&Technology,2010,11(11-12):65-67.
[13]BI Y,LI YC,GE YH.Induced resistance in postharvest fruits and vegetables by chemicals and its mechanism[J].Stewart Postharvest Review,2007,(6):1-7.
[14]WU B(吴斌),WANG FL(王建富),SUN RL(孙瑞林),et al.Potato pest occurrence and control in Taixing City(泰兴市马铃薯病虫害发生特点与防治技术)[J].Modern Agricultural Sciences and Technology(现代农业科技),2011,(14):179-180.
[15]THEV DJ,LIAO XL(廖晓兰).Effects of temperature on dry occurrence in potatoes inoculated with different Fusarium species(温度对接种不同镰刀菌的马铃薯干腐病发展的影响)[J].Rain Fed Crops(国外农学:杂粮作物),1991(6):30-33.
[16]HOU ZY(侯忠艳).Occurrence and control of Fusarium dry rot in potato(马铃薯干腐病的发生与防治)[J].Modern Agricultural Sciences and Technology(现代农业科技),2012,(10):173-179.
[17]MIN FX(闵凡祥),WANG XD(王晓丹),HU LS(胡林双),et al.Identification of species and pathogenicity of the Fusarium on potato in Heilongjiang Province(黑龙江省马铃薯干腐病菌种类鉴定及致病性)[J].Plant Protection(植物保护),2010,(4):112-115.
[18]LI FL(李凤兰),JIANG XF(蒋先锋),SHI LJ(史丽娟),et al.Isolation and identification of two pathogenic stains associated with potato dry rot(两株马铃薯干腐病病原菌的分离和鉴定)[J].Crops(作物杂志),2013,4:125-127.
[19]WANG QM(汪俏梅),GUO DP(郭得平),KYIKYI WIN.Effect of 1-MCP on storage life,quality and antioxidant enzyme activities of broccoli(1-MCP对青花菜贮藏寿命、品质和抗氧化酶活性的影响)[J].Journal of Zhejiang University(Agriculture&Life Sciences)(园艺学报:农业与生命科学版),2004,31(2):205-209.
[20]BRISSON LF,TENHAKEN R,LAMB CJ.Function of oxidative cross-linking of cell wall structural proteins in plant disease resistance[J].Plant Cell,1994,6:1703-1712.
[21]HU JJ(胡景江),LIU ST(刘树滔),PAN JR(潘剑茹).Research progress on technologies of SOD property improvement(SOD特性改善技术研究进展)[J].Strait Pharmaceutical Journal(海峡药学),2009,21(2):11-14.
[22]LI JH(李金华),WANG F(王丰),LIAO YL(廖亦龙).Advance on seed vigor physiological-biochemical and genetic mechanisms in rice(水稻种子活力的生理生化及遗传研究)[J].Molecular Plant Breeding(分子植物育种),2009,7(4):772-777.
[23]WANG JY(王晶英),ZHAO YS(赵雨森),WANG Z(王臻),et al.Effect of drought stress on physiologic and biochemical characteristic of Populus alba×Populus berolinensis(干旱胁迫对银中杨生理生化特性的影响)[J].Journal of Soil and Water Conservation(水土保持学报),2006,20(1):197-200.
[24]JIAO LL(缴丽莉),NI ZY(倪志云),LU BS(路丙社),et al.Effects of low temperature on cold-resistance of David maple(低温胁迫对青榨槭幼树抗寒指标的影响)[J].Journal of Agricultural University of Hebei(河北农业大学学报),2006,29(4):44-47.
[25]ZHANG SN(张蜀宁),ZHANG ZC(张振超),ZHANG HL(张红亮),et al.Growth,physiological and biochemical characteristics of non-heading Chinese cabbage under low temperature stress(低温胁迫对不同倍性不结球白菜生长及生理生化特征的影响)[J].Acta Botanica Boreali-Occidentalia Sinica(西北植物学报),2008,28(1):109-112.
[26]ZHAO SJ(赵世杰),XU ZC(许长成),ZOU Q(邹琦),et al.Improved method for determining malondialdehyde content in plant tissues(植物组织中丙二醛测定方法的改进)[J].Plant Physiology Communications(植物生理学通讯),1994,(3):207-210.
[2]SUN DS(孙东升),LIU GH(刘合光).The analysis and prospect outlook of China's potato industry(我国马铃薯产业发展现状及前景展望)[J].Agricultural Outlook(农业展望),2009,(3):25-28.
[3]SONG BF(宋伯符).Promising potato industry in China(充满朝阳的中国马铃薯产业)[A].Potato industry and high technologies(马铃薯产业与高新技术)[C].Harbin:Harbin Engineering University Press(哈尔滨:哈尔滨工程大学出版社),2002:185-188.
[4]QU DY(屈冬玉),XIE KY(谢开云),JIN LP(金黎平),et al.Development of potato industry and food security in China(中国马铃薯产业发展与食物安全)[J].Scientia Agricultura Sinica(中国农业科学),2005,(2):258-262.
[5]TIAN F(田芳).Occurrence and control of diseases of potato in cellars in Northern China(北方地区马铃薯窖藏病害的发生及防治)[J].Guide of Sci-tech Magazine(科技致富向导),2012,(27):378-402.
[6]CARNEGIE SF,RUTHVEN AD,LINDSAY DA,et al.Effects of fungicides applied to seed potato at harvest or after grading on fungal storage diseases and plant development[J].Ann Appl Biol,1990,116:61-72.
[7]ZHAO SS(赵生山),NIU LH(牛乐华).Survey on potato disease and chemical control during storage(马铃薯贮藏期病害调查及药剂防治研究)[J].Information of Agricultural Science and Technology(农业科技与信息),2008,(11):44-46.
[8]PENG XW(彭学文),ZHU WH(朱杰华).Fungal diseases and distribution of in potato in Hebei Province(河北省马铃薯真菌病害种类及分布)[J].Chinese Potato Journal(中国马铃薯),2008,22(1):31-33.
[9]LIU CH(刘程惠),HU WZ(胡文忠).Physio-biochemical changes of freshcut potato at different storage temperatures(不同贮藏温度下鲜切马铃薯的生理生化变化)[J].Food&Machinery(食品与机械),2008,24(2):38-42.
[10]LI GL(李国龙),WU HX(吴海霞),WEN L(温丽).Progress in physiological and molecular mechanism of drought resistant in crop(作物抗旱生理与分子作用机制研究进展)[J].Chinese Agricultural Science Bulletin(中国农学通报),2010,40(23):185-191.
[11]CHEN Q,SUN HS,YANG JL,et al.Inheritance and molecular mapping of new genes conferring late blight and CPB resistance in Mexican wild potato species Solanum pinnatisectum[J].Agricultural Science&Technology,2009(5):257-261.
[12]ZHANG RJ,CHEN YJ,MENG Ml,et al.Regulation of nitrogen on potato under Na Cl stress[J].Agricultural Science&Technology,2010,11(11-12):65-67.
[13]BI Y,LI YC,GE YH.Induced resistance in postharvest fruits and vegetables by chemicals and its mechanism[J].Stewart Postharvest Review,2007,(6):1-7.
[14]WU B(吴斌),WANG FL(王建富),SUN RL(孙瑞林),et al.Potato pest occurrence and control in Taixing City(泰兴市马铃薯病虫害发生特点与防治技术)[J].Modern Agricultural Sciences and Technology(现代农业科技),2011,(14):179-180.
[15]THEV DJ,LIAO XL(廖晓兰).Effects of temperature on dry occurrence in potatoes inoculated with different Fusarium species(温度对接种不同镰刀菌的马铃薯干腐病发展的影响)[J].Rain Fed Crops(国外农学:杂粮作物),1991(6):30-33.
[16]HOU ZY(侯忠艳).Occurrence and control of Fusarium dry rot in potato(马铃薯干腐病的发生与防治)[J].Modern Agricultural Sciences and Technology(现代农业科技),2012,(10):173-179.
[17]MIN FX(闵凡祥),WANG XD(王晓丹),HU LS(胡林双),et al.Identification of species and pathogenicity of the Fusarium on potato in Heilongjiang Province(黑龙江省马铃薯干腐病菌种类鉴定及致病性)[J].Plant Protection(植物保护),2010,(4):112-115.
[18]LI FL(李凤兰),JIANG XF(蒋先锋),SHI LJ(史丽娟),et al.Isolation and identification of two pathogenic stains associated with potato dry rot(两株马铃薯干腐病病原菌的分离和鉴定)[J].Crops(作物杂志),2013,4:125-127.
[19]WANG QM(汪俏梅),GUO DP(郭得平),KYIKYI WIN.Effect of 1-MCP on storage life,quality and antioxidant enzyme activities of broccoli(1-MCP对青花菜贮藏寿命、品质和抗氧化酶活性的影响)[J].Journal of Zhejiang University(Agriculture&Life Sciences)(园艺学报:农业与生命科学版),2004,31(2):205-209.
[20]BRISSON LF,TENHAKEN R,LAMB CJ.Function of oxidative cross-linking of cell wall structural proteins in plant disease resistance[J].Plant Cell,1994,6:1703-1712.
[21]HU JJ(胡景江),LIU ST(刘树滔),PAN JR(潘剑茹).Research progress on technologies of SOD property improvement(SOD特性改善技术研究进展)[J].Strait Pharmaceutical Journal(海峡药学),2009,21(2):11-14.
[22]LI JH(李金华),WANG F(王丰),LIAO YL(廖亦龙).Advance on seed vigor physiological-biochemical and genetic mechanisms in rice(水稻种子活力的生理生化及遗传研究)[J].Molecular Plant Breeding(分子植物育种),2009,7(4):772-777.
[23]WANG JY(王晶英),ZHAO YS(赵雨森),WANG Z(王臻),et al.Effect of drought stress on physiologic and biochemical characteristic of Populus alba×Populus berolinensis(干旱胁迫对银中杨生理生化特性的影响)[J].Journal of Soil and Water Conservation(水土保持学报),2006,20(1):197-200.
[24]JIAO LL(缴丽莉),NI ZY(倪志云),LU BS(路丙社),et al.Effects of low temperature on cold-resistance of David maple(低温胁迫对青榨槭幼树抗寒指标的影响)[J].Journal of Agricultural University of Hebei(河北农业大学学报),2006,29(4):44-47.
[25]ZHANG SN(张蜀宁),ZHANG ZC(张振超),ZHANG HL(张红亮),et al.Growth,physiological and biochemical characteristics of non-heading Chinese cabbage under low temperature stress(低温胁迫对不同倍性不结球白菜生长及生理生化特征的影响)[J].Acta Botanica Boreali-Occidentalia Sinica(西北植物学报),2008,28(1):109-112.
[26]ZHAO SJ(赵世杰),XU ZC(许长成),ZOU Q(邹琦),et al.Improved method for determining malondialdehyde content in plant tissues(植物组织中丙二醛测定方法的改进)[J].Plant Physiology Communications(植物生理学通讯),1994,(3):207-210.