超声波辅助提取红树莓籽中原花青素及其抗紫外活性评价
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摘要
优化了红树莓籽原花青素的超声波辅助提取工艺,并对所得原花青素的抗紫外活性进行评价。在单因素试验基础上,以原花青素得率为响应值,利用Box-Benhnken设计对影响原花青素得率的超声功率、提取时间、乙醇体积分数和超声温度4个主要因素进行优化;以防晒指数(SPF)为指标,利用紫外分光光度法对原花青素抗紫外活性进行评价。结果表明:超声波辅助提取红树莓籽原花青素的最佳工艺参数为超声功率180 W、提取温度48℃、乙醇体积分数75%、提取时间30 min,该条件下原花青素得率可达14.58 mg/g;红树莓籽原花青素具有较强的抗紫外能力,质量浓度为2 mg/mL时其SPF可达42.84远高于市售化妆品实际SPF。
The ultrasonic-assisted extraction on red raspberry seed proanthocyanidins(RSPC) was optimized by response surface methodology(RSM),also the anti-ultraviolet ability of RSPC was evaluated in this research.Based on single-factor test and Box-Benhnken central combination design,extraction rate of RSPC was taken as index,effects of four crucial factors(i.e.ultrasonic power,extraction time,ethanol concentration and ultrasonic temperature) on extraction rate of RSPC were investigated by response surface analysis.And the anti-ultraviolet ability of RSPC was evaluated while sun protection factor(SPF) was taken as index.The optimum extraction conditions that provided the maximum extraction rate(14.58 mg/g) of RSPC were determined as follows:ultrasonic power,180 W;extraction time,30 min;75%(v/v)ethanol;ultrasonic temperature,47 ℃.The SPF of 2 mg/mL RSPC in ethanol was 42.84,which confirmed the high antiultraviolet ability of RSPC.
The ultrasonic-assisted extraction on red raspberry seed proanthocyanidins(RSPC) was optimized by response surface methodology(RSM),also the anti-ultraviolet ability of RSPC was evaluated in this research.Based on single-factor test and Box-Benhnken central combination design,extraction rate of RSPC was taken as index,effects of four crucial factors(i.e.ultrasonic power,extraction time,ethanol concentration and ultrasonic temperature) on extraction rate of RSPC were investigated by response surface analysis.And the anti-ultraviolet ability of RSPC was evaluated while sun protection factor(SPF) was taken as index.The optimum extraction conditions that provided the maximum extraction rate(14.58 mg/g) of RSPC were determined as follows:ultrasonic power,180 W;extraction time,30 min;75%(v/v)ethanol;ultrasonic temperature,47 ℃.The SPF of 2 mg/mL RSPC in ethanol was 42.84,which confirmed the high antiultraviolet ability of RSPC.
引文
[1]张佰清,闫冬雪.薄层及凝胶色谱法测定树莓籽原花青素的平均聚合度[J].食品工业科技,2013,34(11):317-319.
[2]韩泽梅,李晓波,张玉平,等.树莓种子原花青素的提取分离工艺研究[J].天然产物研究与开发,2006,18(1):108-111.
[3]张佰清,张艳艳,李龙杰.二次柱层析制备高纯度树莓籽原花青素的工艺[J].食品科学,2011,32(8):163-166.
[4]YAHARA N,TOFANI I,MAKI K,et al.Mechanical assessment of effects of grape seed proanthocyanidins extract ontibial bone diaphysis in rats[J].Musculoskelet Neuronal Interact,2005,5(2):162-169.
[5]国植,徐莉.原花青素:具有广阔发展前景的植物药[J].国外医药:植物药分册,1996,11(5):196-204.
[6]张慧文,张玉,马超美.原花青素的研究进展[J].食品科学,2015,36(5):296-304.
[7]秦菲,刘蕊,王龙.超声波辅助提取山竹果皮中的原花青素[J].食品与发酵工业,2012,38(7):157-160.
[8]陈婵,黄琼,彭宏,等.紫色甘薯原花青素稳定性及抗氧化性的研究[J].安徽农业科学,2012,40(35):17308-17310.
[9]张蕊红,齐永琴,邓天昇,等.脱脂沙棘籽渣中原花青素的提纯与稳定性研究[J].食品工业科技,2011,32(8):310-313.
[10]曹小华,肖铎.防晒霜紫外线透光率的测定及防晒效果分析[J].光谱学与光谱分析,2013,33(11):3098-3100.
[11]杨晓冉,郑红艳,董益阳,等.两种3T3光毒性试验方法在防晒化妆品光毒性评价中的应用[J].中国组织工程研究与临床康复,2009,34(13):6765-6768.
[12]KAUR C,SARAF S.Photochemoprotective activity of alcoholic extract of Camellia sinensis[J].International Journal of Pharmacology,2011,7(7):400-404.
[13]H BENDOV魣,J AKRMAN,A KREJC姚魱,et al.In vitro approaches to evaluation of Sun Protection Factor[J].Toxicology in Vitro,2007,21(7):1268-1275.
[14]DUTRA E A,KEDOR-HACKMANN E R M.Determination of sun protection factor(SPF)of sunscreens by ultraviolet spectrophotometry[J].Revista Brasileira De Ciências Farmacêuticas,2004,40(3):381-385.
[15]李春阳,许时婴,王璋.香草醛-盐酸法测定葡萄籽,梗中原花青素含量的研究[J].食品科学,2004,25(2):157-161.
[16]SUDHAHAR V,BALASUBRAMANIAN V.Sun production factor(SPF)determination of marketed sunscreen formulation by In-Vitro method using UV-VIS spectrophotometer[J].Archives of Applied Science Research,2013,5(6):119-122.
[17]MANSUR J,BREDER M,MANSUR M,et al.Determination of sun protection factor by spectrophotometry[J].Anais Brasileiros de Dermatologia,1986,61(3):121-124.
[18]SAYRE R M,AGIN P P,LEVEE G J,et al.Acomparison of in vivo and in vitro testing of sunscreening formulas[J].Photochemistry and Photobiology,1979,29(3):559-566.
[19]孙晓晨,张方,邵华.紫外线对人体的影响[J].中国职业医学,2016,43(3):380-383.
[20]MISHRA A K,MISHRA A,CHATTOPADHYAY PAssessment of In Vitro sun protection factor of Calendula officinalis L.(Asteraceae)essential oil formulation[J].Journal of Young Pharmacists,2012,4(1):17-21.
[21]FONSECA A P,NOBRE R.Determination of sun protection factor by UV-Vis spectrophotometry[J].Health Care Current Reviews,2013,1(1):1-4.
[22]杨健,袁春龙,任亚梅,等.葡萄籽原花青素的提取及其抗紫外线活性测定[J].食品科学,2014,35(18):69-73.
[2]韩泽梅,李晓波,张玉平,等.树莓种子原花青素的提取分离工艺研究[J].天然产物研究与开发,2006,18(1):108-111.
[3]张佰清,张艳艳,李龙杰.二次柱层析制备高纯度树莓籽原花青素的工艺[J].食品科学,2011,32(8):163-166.
[4]YAHARA N,TOFANI I,MAKI K,et al.Mechanical assessment of effects of grape seed proanthocyanidins extract ontibial bone diaphysis in rats[J].Musculoskelet Neuronal Interact,2005,5(2):162-169.
[5]国植,徐莉.原花青素:具有广阔发展前景的植物药[J].国外医药:植物药分册,1996,11(5):196-204.
[6]张慧文,张玉,马超美.原花青素的研究进展[J].食品科学,2015,36(5):296-304.
[7]秦菲,刘蕊,王龙.超声波辅助提取山竹果皮中的原花青素[J].食品与发酵工业,2012,38(7):157-160.
[8]陈婵,黄琼,彭宏,等.紫色甘薯原花青素稳定性及抗氧化性的研究[J].安徽农业科学,2012,40(35):17308-17310.
[9]张蕊红,齐永琴,邓天昇,等.脱脂沙棘籽渣中原花青素的提纯与稳定性研究[J].食品工业科技,2011,32(8):310-313.
[10]曹小华,肖铎.防晒霜紫外线透光率的测定及防晒效果分析[J].光谱学与光谱分析,2013,33(11):3098-3100.
[11]杨晓冉,郑红艳,董益阳,等.两种3T3光毒性试验方法在防晒化妆品光毒性评价中的应用[J].中国组织工程研究与临床康复,2009,34(13):6765-6768.
[12]KAUR C,SARAF S.Photochemoprotective activity of alcoholic extract of Camellia sinensis[J].International Journal of Pharmacology,2011,7(7):400-404.
[13]H BENDOV魣,J AKRMAN,A KREJC姚魱,et al.In vitro approaches to evaluation of Sun Protection Factor[J].Toxicology in Vitro,2007,21(7):1268-1275.
[14]DUTRA E A,KEDOR-HACKMANN E R M.Determination of sun protection factor(SPF)of sunscreens by ultraviolet spectrophotometry[J].Revista Brasileira De Ciências Farmacêuticas,2004,40(3):381-385.
[15]李春阳,许时婴,王璋.香草醛-盐酸法测定葡萄籽,梗中原花青素含量的研究[J].食品科学,2004,25(2):157-161.
[16]SUDHAHAR V,BALASUBRAMANIAN V.Sun production factor(SPF)determination of marketed sunscreen formulation by In-Vitro method using UV-VIS spectrophotometer[J].Archives of Applied Science Research,2013,5(6):119-122.
[17]MANSUR J,BREDER M,MANSUR M,et al.Determination of sun protection factor by spectrophotometry[J].Anais Brasileiros de Dermatologia,1986,61(3):121-124.
[18]SAYRE R M,AGIN P P,LEVEE G J,et al.Acomparison of in vivo and in vitro testing of sunscreening formulas[J].Photochemistry and Photobiology,1979,29(3):559-566.
[19]孙晓晨,张方,邵华.紫外线对人体的影响[J].中国职业医学,2016,43(3):380-383.
[20]MISHRA A K,MISHRA A,CHATTOPADHYAY PAssessment of In Vitro sun protection factor of Calendula officinalis L.(Asteraceae)essential oil formulation[J].Journal of Young Pharmacists,2012,4(1):17-21.
[21]FONSECA A P,NOBRE R.Determination of sun protection factor by UV-Vis spectrophotometry[J].Health Care Current Reviews,2013,1(1):1-4.
[22]杨健,袁春龙,任亚梅,等.葡萄籽原花青素的提取及其抗紫外线活性测定[J].食品科学,2014,35(18):69-73.