马铃薯茎段高频再生体系的建立
|
摘要
以大西洋、春薯3号、春薯5号、吉薯1号马铃薯脱毒试管苗为试验材料,对茎段再生体系进行研究,筛选与优化马铃薯再生体系的条件,以期建立马铃薯高频再生体系。结果表明:大西洋的愈伤组织诱导及分化效果最佳,诱导的愈伤组织呈鲜绿色,诱导出芽用时最短,诱导率和分化率分别为100%和95%。春薯3号、春薯5号、吉薯1号的分化率分别达79.4%、68.75%、85.7%。其中在添加2.0 mg/L 6-BA+0.5 mg/L 2,4-D+0.4 mg/L KT的MS培养基中,四个品种马铃薯的茎段均能形成愈伤组织,20 d愈伤组织诱导率均达100%。
The detoxification seedlings of potato cultivars‘Atlantic',‘Chunshu 3',‘Chunshu 5'and‘Jishu 1'were used as experimental materials, and the conditions of their stem tissue regeneration were explored and optimized with an aim to establish an efficient regeneration protocol in potato. The results showed that‘Atlantic'exhibited advantages over the 4 cultivars tested in terms of callus induction and differentiation. Under our experimental condition, Atlantic's stem tissue sprout quickly and differentiated into callus of bright green with an induction rate and differentiation rate of 100% and 95%, respectively. The differentiation rates of other 3 cultivars were estimated to be 79.4% for‘Chunshu 3', 68.75% for‘Chunshu 5'and 85.7% for‘Jishu 1'. Using the MS medium supplemented with 2.0 mg/L 6-BA + 0.5 mg/L 2, 4-D + 0.4 mg/L KT, the stem tissue of the 4 potato cultivars may be differentiated completely into callus within 20 days.
The detoxification seedlings of potato cultivars‘Atlantic',‘Chunshu 3',‘Chunshu 5'and‘Jishu 1'were used as experimental materials, and the conditions of their stem tissue regeneration were explored and optimized with an aim to establish an efficient regeneration protocol in potato. The results showed that‘Atlantic'exhibited advantages over the 4 cultivars tested in terms of callus induction and differentiation. Under our experimental condition, Atlantic's stem tissue sprout quickly and differentiated into callus of bright green with an induction rate and differentiation rate of 100% and 95%, respectively. The differentiation rates of other 3 cultivars were estimated to be 79.4% for‘Chunshu 3', 68.75% for‘Chunshu 5'and 85.7% for‘Jishu 1'. Using the MS medium supplemented with 2.0 mg/L 6-BA + 0.5 mg/L 2, 4-D + 0.4 mg/L KT, the stem tissue of the 4 potato cultivars may be differentiated completely into callus within 20 days.
引文
[1]王娟,陈佳宁,王娟,等.根癌农杆菌介导马铃薯遗传转化的研究进展[J].安徽农业科学,2010,38(19):10021-10023.
[2]陈萌山,王小虎.中国马铃薯主食产业化发展与展望[J].农业经济问题,2015(12):4-11.
[3]Ren N,Zhang Z W,Yu X C,et al.ROS and salicylic acid(SA)play roles on the resistance establishment of the potato cultivar Zihuabai to Phytophthora infestans[J].Journal of Plant Diseases&Protection,2012,119(5/6):191-199.
[4]柳蓉.马铃薯的再生及其再生植株遗传稳定性研究[D].长沙:湖南农业大学,2007.
[5]李晶.马铃薯再生体系的建立及遗传转化的研究[D].哈尔滨:东北农业大学,2003.
[6]李娟,程慧,张国裕.马铃薯叶片高效再生体系的建立[J].西北植物学报,2004(4):610-614.
[7]杨琼芬,卢丽丽,潘哲超,等.马铃薯叶片愈伤组织再生体系的建立[J].西南农业学报,2012(3):1001-1004.
[8]鲍红春,李小雷,王建平,等.马铃薯品种陇薯5号茎段再生体系的建立[J].内蒙古农业科技,2014(2):31-32.
[9]卢翠华,秦昕,武小霞,等.马铃薯极早熟品种东农303再生系统的筛选[J].中国马铃薯,2001(5):280-281.
[10]王克秀,何卫,胡建军,等.马铃薯脱毒组培苗繁殖效率分析研究[J].西南农业学报,2010,23(3):660-664.
[11]帅正彬,郭江洪,杨斌,等.不同培养条件对马铃薯试管薯诱导的影响[J].西南农业学报,2004(2):212-214.
[12]李文霞,宁海龙,张永根,等.二倍体马铃薯花药培养再生体系的优化[J].中国蔬菜,2012(4):68-74.
[13]Shaw R.Potato tuber callus validation as biochemical tool[J].Plant Physiol,1976,58:464-467.
[14]Simmonds N W.Observation on potato callus and adventitious shoot formation[J].American Potato Journal,1964,4:129-136.
[15]李娟,程智慧,张国裕.四个马铃薯品种幼茎段再生技术的研究[J].西北农林科技大学学报,2006,34(3):122-125.
[16]Radke S,Grun P.Isolation,culture,and regeneration of leaf mesophyll protoplasts of selected clones of Solanum[J].Potato Research,1986,29:451-462.
[17]周宇波,柳俊,谢从华,等.马铃薯原生质体培养体系改良[J].华中农业大学学报,2001,20(5):469-473.
[18]祁新,赵颖君,王艳秋,等.马铃薯悬浮细胞原生质体培养及植株再生[J].吉林农业大学学报,2000,22(1):52-55.
[19]丁玉梅,杨正安,吴毅歆,等.用于叶绿体基因组转化的马铃薯高效再生体系的建立[J].西南农业学报,2006,19(增刊):98-102.
[20]杨琼芬,隋启君,李世峰,等.马铃薯新品种“云薯201”脱毒种苗生产中的影响因素研究[J].西南农业学报,2006,19(4):679-682.
[21]王宪.二倍体马铃薯再生体系建立及耐盐愈伤组织的筛选[D].哈尔滨:东北农业大学,2012.
[22]刘峰,王凤,汪洋,等.吉林省马铃薯及主要农作物的比较优势分析[J].吉林农业科学,2009,34(5):46-49.
[23]徐仲安,王天保,李常英,等.正交试验设计法简介[J].科技情报开发与经济,2002(5):148-150.
[24]方贯娜,庞淑敏.马铃薯愈伤组织再生体系的研究进展[J].中国马铃薯,2012,26(5):307-310.
[25]张玉英,韦正乙,王云鹏,等.番茄叶片高频再生体系的建立[J].吉林农业科学,2014,39(2):78-82,86.
[2]陈萌山,王小虎.中国马铃薯主食产业化发展与展望[J].农业经济问题,2015(12):4-11.
[3]Ren N,Zhang Z W,Yu X C,et al.ROS and salicylic acid(SA)play roles on the resistance establishment of the potato cultivar Zihuabai to Phytophthora infestans[J].Journal of Plant Diseases&Protection,2012,119(5/6):191-199.
[4]柳蓉.马铃薯的再生及其再生植株遗传稳定性研究[D].长沙:湖南农业大学,2007.
[5]李晶.马铃薯再生体系的建立及遗传转化的研究[D].哈尔滨:东北农业大学,2003.
[6]李娟,程慧,张国裕.马铃薯叶片高效再生体系的建立[J].西北植物学报,2004(4):610-614.
[7]杨琼芬,卢丽丽,潘哲超,等.马铃薯叶片愈伤组织再生体系的建立[J].西南农业学报,2012(3):1001-1004.
[8]鲍红春,李小雷,王建平,等.马铃薯品种陇薯5号茎段再生体系的建立[J].内蒙古农业科技,2014(2):31-32.
[9]卢翠华,秦昕,武小霞,等.马铃薯极早熟品种东农303再生系统的筛选[J].中国马铃薯,2001(5):280-281.
[10]王克秀,何卫,胡建军,等.马铃薯脱毒组培苗繁殖效率分析研究[J].西南农业学报,2010,23(3):660-664.
[11]帅正彬,郭江洪,杨斌,等.不同培养条件对马铃薯试管薯诱导的影响[J].西南农业学报,2004(2):212-214.
[12]李文霞,宁海龙,张永根,等.二倍体马铃薯花药培养再生体系的优化[J].中国蔬菜,2012(4):68-74.
[13]Shaw R.Potato tuber callus validation as biochemical tool[J].Plant Physiol,1976,58:464-467.
[14]Simmonds N W.Observation on potato callus and adventitious shoot formation[J].American Potato Journal,1964,4:129-136.
[15]李娟,程智慧,张国裕.四个马铃薯品种幼茎段再生技术的研究[J].西北农林科技大学学报,2006,34(3):122-125.
[16]Radke S,Grun P.Isolation,culture,and regeneration of leaf mesophyll protoplasts of selected clones of Solanum[J].Potato Research,1986,29:451-462.
[17]周宇波,柳俊,谢从华,等.马铃薯原生质体培养体系改良[J].华中农业大学学报,2001,20(5):469-473.
[18]祁新,赵颖君,王艳秋,等.马铃薯悬浮细胞原生质体培养及植株再生[J].吉林农业大学学报,2000,22(1):52-55.
[19]丁玉梅,杨正安,吴毅歆,等.用于叶绿体基因组转化的马铃薯高效再生体系的建立[J].西南农业学报,2006,19(增刊):98-102.
[20]杨琼芬,隋启君,李世峰,等.马铃薯新品种“云薯201”脱毒种苗生产中的影响因素研究[J].西南农业学报,2006,19(4):679-682.
[21]王宪.二倍体马铃薯再生体系建立及耐盐愈伤组织的筛选[D].哈尔滨:东北农业大学,2012.
[22]刘峰,王凤,汪洋,等.吉林省马铃薯及主要农作物的比较优势分析[J].吉林农业科学,2009,34(5):46-49.
[23]徐仲安,王天保,李常英,等.正交试验设计法简介[J].科技情报开发与经济,2002(5):148-150.
[24]方贯娜,庞淑敏.马铃薯愈伤组织再生体系的研究进展[J].中国马铃薯,2012,26(5):307-310.
[25]张玉英,韦正乙,王云鹏,等.番茄叶片高频再生体系的建立[J].吉林农业科学,2014,39(2):78-82,86.