改性青稞β-葡聚糖荷载黑枸杞花青素微胶囊溶液抗疲劳功能的评价
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摘要
对改性青稞β-葡聚糖荷载黑枸杞花青素微胶囊溶液的抗疲劳功效进行研究。以不同剂量浓度的改性青稞β-葡聚糖荷载黑枸杞花青素微胶囊溶液为受试样品,以SPF级BALB/c小白鼠为试验受体,将其分为低、中、高剂量组、阳性对照组和空白对照组,对各组小鼠以0.2 mL/d的剂量连续灌胃30 d后,进行负重游泳实验,并对小鼠进行不负重游泳后拔眼球采血测定血清尿素氮水平,对小鼠进行解剖测定脏器湿重、肝糖原水平两项指标,采用尾尖取血法对小鼠血乳酸水平进行测试。结果表明:与空白对照组比较,改性青稞β-葡聚糖荷载黑枸杞花青素微胶囊溶液各剂量组均能延长小鼠负重游泳时间,增加肝糖原的储备量,降低小鼠运动后血清尿素氮和血乳酸水平,且剂量浓度为1.0 mg/mL(低剂量组)时,各项测定指标都优于空白对照组(p<0.05)。因此,改性青稞β-葡聚糖荷载黑枸杞花青素微胶囊溶液具有明显的抗疲劳功效,且1.0 mg/mL的浓度为最佳剂量。
Anti-fatigue function of the solution of microencapsulation loading anthocyanidins of Lycium ruthenicum Murr by modified β-glucan from highland barley had been studied. The solution of microencapsulation loading anthocyanidins of Lycium ruthenicum Murr by modified β-glucan from highland barley as ingredient had been concentrated into different dose sample for experiment. SPF BALB/c mice were chosen as the experimental receptor and divided into low, middle, high dose, positive control and blank control group. After gavage for 30 days with 0.2 mL/d dose, the loaded swimming test was conducted, and blood was taken from eyes of mice with no loaded swimming to test serum urea nitrogen level. At the same time, mice had been dissected to test wet weight of their viscera and liver glycogen level. What's more, blood lactic acid level were tested by the method of taking blood from tails. The result showed that the solution of microencapsulation loading anthocyanidins of Lycium ruthenicum Murr could prolong the swimming time compared with the blank control group, increase liver glycogen reserves, reduce serum urea nitrogen and the levels of blood lactic acid in mice after swimming. Every measurement index of experimental group was better than blank control group(p<0.05) when the concentration was 1.0 mg/mL(low dose group). Therefore, the solution of microencapsulation loading anthocyanidins of Lycium ruthenicum Murr by the modified β-glucan from highland barley had significant anti-fatigue function and the concentration of 1.0 mg/mL was the best dose.
Anti-fatigue function of the solution of microencapsulation loading anthocyanidins of Lycium ruthenicum Murr by modified β-glucan from highland barley had been studied. The solution of microencapsulation loading anthocyanidins of Lycium ruthenicum Murr by modified β-glucan from highland barley as ingredient had been concentrated into different dose sample for experiment. SPF BALB/c mice were chosen as the experimental receptor and divided into low, middle, high dose, positive control and blank control group. After gavage for 30 days with 0.2 mL/d dose, the loaded swimming test was conducted, and blood was taken from eyes of mice with no loaded swimming to test serum urea nitrogen level. At the same time, mice had been dissected to test wet weight of their viscera and liver glycogen level. What's more, blood lactic acid level were tested by the method of taking blood from tails. The result showed that the solution of microencapsulation loading anthocyanidins of Lycium ruthenicum Murr could prolong the swimming time compared with the blank control group, increase liver glycogen reserves, reduce serum urea nitrogen and the levels of blood lactic acid in mice after swimming. Every measurement index of experimental group was better than blank control group(p<0.05) when the concentration was 1.0 mg/mL(low dose group). Therefore, the solution of microencapsulation loading anthocyanidins of Lycium ruthenicum Murr by the modified β-glucan from highland barley had significant anti-fatigue function and the concentration of 1.0 mg/mL was the best dose.
引文
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[19]张卓睿,毛迪锐,高晗,等.蓝莓花青素对小鼠抗疲劳及体内抗氧化作用[J].食品科学,2017,38(21):207-211
[20]曹茸茸,杨晓磊,孙敏,等.藏药黑果枸杞对运动疲劳小鼠的缓解作用[J].中国临床药理学杂志,2017,33(04):351-354
[2]古丽达娜,罗琦珍,陶大勇,等.黑果枸杞色素对小鼠常压耐缺氧及游泳耐力的影响[J].时珍国医国药,2009,20(11):2682
[3]李进,原惠,曾献春,等.黑果枸杞色素的毒理学研究[J].食品科学,2007,28(7):470-475
[4]汪建红,陈晓琴,张蔚佼.黑果枸杞果实多糖抗疲劳生物功效及其机制研究[J].食品科技,2009,34(2):203-207
[5]李淑珍,李进.黑果枸杞总黄酮降血脂作用[J].时珍国医国药,2012,(05):1072-1074
[6] Wood P J. Relationship between solution properties of cereal beta-glucans and physiological effects[J]. Trends in Food Science&Technology,2002,13:313-320
[7]宋萍,张雷雷,于军.青稞β-葡聚糖营养作用及其提取工艺的研究[J].中国食物与营养,2008,(8):8-9
[8] Duval-Tenie C, Huguet J, Muller G. Self-assembly and hydrophobic dusters of amphiphilic polysaccharides[J].Colloids and Surfaces A:Physicochemical and Engineering Aspects,2003,220(1):105-115
[9]刘嘉.OS-燕麦β-葡聚糖酯自聚集体的构建及应用[D].重庆:西南大学博士学位论文,2015
[10] Yu H, Huang Q. Enhanced in vitro anti-cancer activity of curcumin encapsulated in hydrophobically modified starch[J]. Food Chemistry,2010,119(2):669-674
[11]黄继汉,黄晓晖,陈志扬,等.药理试验中动物间和动物与人体间的等效剂量换算[J].中国临床药理学与治疗学,2004,(09):1069-1072
[12]江洋,丁龙,王四清.不同产地黑果枸杞中原花青素和花青素含量研究[J].食品工业科技,2016,37(13):122-126
[13]王树林,陈虎,秦艳婷,等.一种微胶囊[P].中国:CN106983149A,2017-07-28
[14]罗琼,阎俊,张声华.枸杞多糖的分离纯化及其抗疲劳作用[J].卫生研究,2007:115-117
[15]卫生部.保健食品检验与评价技术规范(2003版)[M].北京:中华人民共和国卫生部,2003:87-93
[16]程颖,王欣之,吴皓,等.江苏产菲律宾蛤仔提取物的抗疲劳作用[J].食品与生物技术学报,2017,36(01):51-55
[17]何来英,严卫星,楼密密,等.保健食品抗疲劳作用试验方法研究[J].中国食品卫生杂志,1997,9(4):1-6
[18]刘庆春,毕,刘爱兵,等.枣环磷酸腺苷提取液对小鼠抗疲劳作用的药效学研究[J].肠外与肠内营养,2014,21(6):46-48
[19]张卓睿,毛迪锐,高晗,等.蓝莓花青素对小鼠抗疲劳及体内抗氧化作用[J].食品科学,2017,38(21):207-211
[20]曹茸茸,杨晓磊,孙敏,等.藏药黑果枸杞对运动疲劳小鼠的缓解作用[J].中国临床药理学杂志,2017,33(04):351-354