迷迭香精油对金葡菌感染小鼠的干预作用
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摘要
【目的】为了探明迷迭香精油在体内外试验中的抗菌作用。【方法】采用药敏试验检测迷迭香精油体外抑菌效果;通过金黄色葡萄球菌(金葡菌)滴鼻法建立小鼠肺炎模型,小鼠每天2次吸入迷迭香精油,每次30分连续7d,试验结束小鼠称重、采血,生化分析仪分析血常规指标,组织固定制备石蜡切片,显微镜观察组织病变,细菌定殖检测。【结果】迷迭香精油组大肠杆菌、金葡菌和链球菌抑菌圈分别为(12.4±2.4)、(12.6±1.2)、(11.5±0.5)mm,茶树精油有抑菌圈出现,薰衣草精油组无抑菌圈。模型组小鼠金葡菌感染后反应明显增强、采食减少、体重明显减轻,肺指数升高,肺细菌定殖率高,白介素-6 (IL-6)和炎性细胞数量明显增加;肉眼观察到小鼠肺脏肿胀充血、色泽暗红,肺叶上均有大小不等的出血斑,肺组织有硬肿块,肺门淋巴结肿大;镜检发现肺脏结构改变明显,肺泡间隙有炎性细胞聚集和浸润,组织实变明显,肺泡壁增厚,肺泡组织结构完整性破坏,肺泡腔内可见出血。与模型组比较,迷迭香组小鼠反应温和,采食和体重增加,肺脏指数降低和细菌定植率下降,后期IL-6和炎性细胞数量明显减少;小鼠肺组织充血减轻,肺泡间隙炎性细胞浸润减少,肺泡结构完整性逐步恢复,接近对照组小鼠。【结论】迷迭香精油体外具有良好的抗菌作用,体内缓解金葡菌引起的肺炎炎症症状。
【Objective】It is aimed to investigate the antibacterial effects of rosemary essential oil by both in vivo and in vitro experiments.【Methods】The antibacterial effects of rosemary essential oil in vitro were detected by drug susceptibility test.Mouse pneumonia model was established by staphylococcus aureus nasal drip.Then the mice smell rosemary essential oil twice a day,30 min every time,for seven consecutive days.Tissue sections were prepared by tissue fixation,tissue lesions were observed under microscope,and bacterial colonization was also detected.【Results】The E.coli,Staphylococcus aureus and Streptococcus in the rosemary essential oil group had obvious inhibition zones of 12.4±2.4,12.6±1.2 and 11.5±0.5(mm),respectively.The tea tree oil had an inhibition zone,but the lavender group did not.In the model group,the response of mice with staphylococcus aureus infection was significantly increased,but the amount of IL-6 and inflammatory cells significantly increased.Their lung looked likes welling,hyperemia and dark red color.There were blood spots of different sizes on the lungs,hard masses in the lung tissue and enlarged hilar lymph nodes.The obvious changes in lung structure,inflammatory cell aggregation and infiltration in the alveolar space were observed by microscope,as well as obvious tissue consolidation,thickening of alveolar wall,destruction of alveolar structure integrity,and bleeding in alveolar cavity.Compared with the model group,the mice in rosemary group showed mild reaction,food intake and bodyweight increasing,and decreased their lung index and bacterial colonization rate,and significantly decreased IL-6 and inflammatory cells in the later stage.There was a decrease in lung tissue hyperemia in mice,inflammatory cell infiltration in alveolar space,and a gradually recovery in alveolar structural integrity which was close to those in the control one.【Conclusion】Rosemary essential oil has a good antibacterial effect in vitro and can relieve the inflammation of the mice pneumonia caused by staphylococcus aureus in vivo.
【Objective】It is aimed to investigate the antibacterial effects of rosemary essential oil by both in vivo and in vitro experiments.【Methods】The antibacterial effects of rosemary essential oil in vitro were detected by drug susceptibility test.Mouse pneumonia model was established by staphylococcus aureus nasal drip.Then the mice smell rosemary essential oil twice a day,30 min every time,for seven consecutive days.Tissue sections were prepared by tissue fixation,tissue lesions were observed under microscope,and bacterial colonization was also detected.【Results】The E.coli,Staphylococcus aureus and Streptococcus in the rosemary essential oil group had obvious inhibition zones of 12.4±2.4,12.6±1.2 and 11.5±0.5(mm),respectively.The tea tree oil had an inhibition zone,but the lavender group did not.In the model group,the response of mice with staphylococcus aureus infection was significantly increased,but the amount of IL-6 and inflammatory cells significantly increased.Their lung looked likes welling,hyperemia and dark red color.There were blood spots of different sizes on the lungs,hard masses in the lung tissue and enlarged hilar lymph nodes.The obvious changes in lung structure,inflammatory cell aggregation and infiltration in the alveolar space were observed by microscope,as well as obvious tissue consolidation,thickening of alveolar wall,destruction of alveolar structure integrity,and bleeding in alveolar cavity.Compared with the model group,the mice in rosemary group showed mild reaction,food intake and bodyweight increasing,and decreased their lung index and bacterial colonization rate,and significantly decreased IL-6 and inflammatory cells in the later stage.There was a decrease in lung tissue hyperemia in mice,inflammatory cell infiltration in alveolar space,and a gradually recovery in alveolar structural integrity which was close to those in the control one.【Conclusion】Rosemary essential oil has a good antibacterial effect in vitro and can relieve the inflammation of the mice pneumonia caused by staphylococcus aureus in vivo.
引文
[1]吴修仁.潮汕生物资源植物志略[M].广州:中山大学出版社,1997:281
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[3]赵献敏,李南,杜彩霞,等.天王补心丹联合迷迭香精油对阿尔兹海默病模型小鼠记忆功能的影响[J].中医学报,2018,33(04):611-615
[4]佟棽棽,姚雷.迷迭香和柠檬草的精油以及活体香气的抗抑郁作用的研究[J].上海交通大学学报(农业科学版),2009,27(1):82-85
[5]顾仁勇,张丽,傅伟昌,等.芭蕉汁的抑菌作用[J].食品与发酵工业,2005,31(3):57-59
[6]Ojeda-Sana,M Adriana,Moreno,Silvia,et al.New insights into antibacterial and antioxidant activities of rosemary,essential oils and their main components[J].Food Control,2013,31(1):189-195
[7]孔静思,陈季武,王帮正,等.三种芳香植物抑菌比较及GC/MS分析[J].食品工业科技,2011,32(11):151-155
[8]D A Silva Bomfim N,Nakassugi L P,Oliveira J F P,et al.Antifungal activity and inhibition of fumonisin production by Rosmarinus officinalis L.essential oil in Fusariumver ticillioides(Sacc.)Nirenberg[J].Food Chemistry,2015,166:330-336
[9]陈陵,赵学成,邓琼,等.纳米氧化铈急性染毒对雄性小鼠体重和脏器系数及血常规的影响[J].环境与健康杂志,2010,27(10):899-901
[10]田小芸,恽时锋,周森妹,等.C57BL KsJ-dbdb、+db小鼠主要脏器重量及脏器系数的测定[J].中国比较医学杂志,2006,16(4):220-222
[11]马秀兰,宋淑云,叶建新,等.对成年大鼠脏器系数的探讨[J].动物学志,1996,31(3):25-26
[12]沈虹.精油的芳香疗法[J].日用化学品科学,2011,34(9):42-46
[13]汪攀,胡婷,褚衍伟,等.约氏乳杆菌对大鼠生长性能、血液指标及脏器指数的影响[J].动物健康,2017,53(3):112-116
[14]Hlorenzo,Oquesada,Passuncao,et al.Cytokine expression in porcine lungs experimentally infected with Mycoplasma hyopneumoniae[J].Vet Immunol Immuno Pathol,2006,109(3-4):199-207
[15]Hendeman,Scameijvis,Gtrijkers,et al.Systemic cytokine response in patients with community-acquired pneumonia[J].Eur Respir,2011,37(6):1431-1438
[16]姚娜,李树德,王文林.IL-6,IL-10在实验性肺部炎症中的研究进展[J].昆明医科大学学报,2014,35(3):161-163
[17]Zobel K,Martus P,Pletz M W,et al.Interleukin 6,lipopolysaccharide-binding protein and interleukin 10in the prediction of risk and etiologic patterns in patients with communityacquired pneumonia:results from the German competence network Capnetz[J].BMC PμLm Med,2012,20(12):6
[18]Lee Y L,Chen W,Chen L Y,etal.Systemic and Bronchoalveolar cytokines as predictors of inhospital mortality in severe community-acquired pneumonia[J].J Crit Care,2010,25(1):7-13
[19]Amaral G P,De Carvalho N R,Barcelos R P,et al.Protective action of ethanolic extract of Rosmarinus officinalis L.in gastric ulcer prevention induced by ethanol in rats[J].Journal of Clinical Oncology,2012,55:48-55
[2]孔静思.三种芳香植物抑菌、抗氧化活性研究及GC/MS分析[D].上海:华东师范大学,2011
[3]赵献敏,李南,杜彩霞,等.天王补心丹联合迷迭香精油对阿尔兹海默病模型小鼠记忆功能的影响[J].中医学报,2018,33(04):611-615
[4]佟棽棽,姚雷.迷迭香和柠檬草的精油以及活体香气的抗抑郁作用的研究[J].上海交通大学学报(农业科学版),2009,27(1):82-85
[5]顾仁勇,张丽,傅伟昌,等.芭蕉汁的抑菌作用[J].食品与发酵工业,2005,31(3):57-59
[6]Ojeda-Sana,M Adriana,Moreno,Silvia,et al.New insights into antibacterial and antioxidant activities of rosemary,essential oils and their main components[J].Food Control,2013,31(1):189-195
[7]孔静思,陈季武,王帮正,等.三种芳香植物抑菌比较及GC/MS分析[J].食品工业科技,2011,32(11):151-155
[8]D A Silva Bomfim N,Nakassugi L P,Oliveira J F P,et al.Antifungal activity and inhibition of fumonisin production by Rosmarinus officinalis L.essential oil in Fusariumver ticillioides(Sacc.)Nirenberg[J].Food Chemistry,2015,166:330-336
[9]陈陵,赵学成,邓琼,等.纳米氧化铈急性染毒对雄性小鼠体重和脏器系数及血常规的影响[J].环境与健康杂志,2010,27(10):899-901
[10]田小芸,恽时锋,周森妹,等.C57BL KsJ-dbdb、+db小鼠主要脏器重量及脏器系数的测定[J].中国比较医学杂志,2006,16(4):220-222
[11]马秀兰,宋淑云,叶建新,等.对成年大鼠脏器系数的探讨[J].动物学志,1996,31(3):25-26
[12]沈虹.精油的芳香疗法[J].日用化学品科学,2011,34(9):42-46
[13]汪攀,胡婷,褚衍伟,等.约氏乳杆菌对大鼠生长性能、血液指标及脏器指数的影响[J].动物健康,2017,53(3):112-116
[14]Hlorenzo,Oquesada,Passuncao,et al.Cytokine expression in porcine lungs experimentally infected with Mycoplasma hyopneumoniae[J].Vet Immunol Immuno Pathol,2006,109(3-4):199-207
[15]Hendeman,Scameijvis,Gtrijkers,et al.Systemic cytokine response in patients with community-acquired pneumonia[J].Eur Respir,2011,37(6):1431-1438
[16]姚娜,李树德,王文林.IL-6,IL-10在实验性肺部炎症中的研究进展[J].昆明医科大学学报,2014,35(3):161-163
[17]Zobel K,Martus P,Pletz M W,et al.Interleukin 6,lipopolysaccharide-binding protein and interleukin 10in the prediction of risk and etiologic patterns in patients with communityacquired pneumonia:results from the German competence network Capnetz[J].BMC PμLm Med,2012,20(12):6
[18]Lee Y L,Chen W,Chen L Y,etal.Systemic and Bronchoalveolar cytokines as predictors of inhospital mortality in severe community-acquired pneumonia[J].J Crit Care,2010,25(1):7-13
[19]Amaral G P,De Carvalho N R,Barcelos R P,et al.Protective action of ethanolic extract of Rosmarinus officinalis L.in gastric ulcer prevention induced by ethanol in rats[J].Journal of Clinical Oncology,2012,55:48-55