复合菌剂强化处理高盐废水脱氮效果
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摘要
将耐盐脱氮复合菌剂投加到序批式生物反应器中,构建生物强化高盐废水处理系统(SBR1),以未投加复合菌剂系统(SBR2)作为对照,分析典型周期中氮素和溶解氧的变化趋势以及盐度冲击对脱氮效果的影响。实验表明,在曝气时间为6 h时,生物强化系统脱氮率可稳定在96%以上,出水总氮浓度为3.8 mg/L左右。反应中始终无硝氮、亚硝氮积累,生物强化系统具有同步硝化好氧反硝化能力。当受到5%和7%较高盐度冲击时,生物强化系统表现出优于对照系统的抗盐度冲击能力,能够快速恢复原有活性,且出水总氮低于15 mg/L;当受到0%盐度的淡水冲击时,对照系统中耐盐污泥失活且无法恢复,而生物强化系统只需投加少量(3%)耐盐脱氮复合菌剂,即可快速恢复活性,出水总氮低于15 mg/L。本研究能够为生物强化高盐废水脱氮系统的构建和运行提供技术支持。
Salt-tolerant denitrification composite microbial inoculum were added into sequencing batch reactor to develop a bioaugmentation system( SBR1),taking a system without composite microbial inoculum( SBR2) as control. This study compared and analyzed their transformation of nitrogen and changes of dissolved oxygen in typical operation cycle and the performance under salinity shock. The results demonstrated that when the aeration time was 6 hours,the denitrification rate maintained above 96% in the bioaugmentation system,and the total nitrogen of effluent was around 3. 8 mg / L. Meanwhile,nitrate and nitrite always had no accumulation,the bioaugmentation system had the capacity of simultaneous nitrification and aerobic denitrification. When facing salinity shock of 5% and 7%,the bioaugmentation system had remarkable performance. After quick recovery,its effluent total nitrogen was below 15 mg / L. When shocked by 0% salinity,the control system was failed and unable to restore. While the bioaugmentation system recovered quickly by inoculating 3% of salt-tolerant denitrification composite microbial inoculum,and total nitrogen of effluent dropped to below 15 mg / L again. This research could provide technical support for construction and operation of bioaugmentation saline wastewater denitrification system.
Salt-tolerant denitrification composite microbial inoculum were added into sequencing batch reactor to develop a bioaugmentation system( SBR1),taking a system without composite microbial inoculum( SBR2) as control. This study compared and analyzed their transformation of nitrogen and changes of dissolved oxygen in typical operation cycle and the performance under salinity shock. The results demonstrated that when the aeration time was 6 hours,the denitrification rate maintained above 96% in the bioaugmentation system,and the total nitrogen of effluent was around 3. 8 mg / L. Meanwhile,nitrate and nitrite always had no accumulation,the bioaugmentation system had the capacity of simultaneous nitrification and aerobic denitrification. When facing salinity shock of 5% and 7%,the bioaugmentation system had remarkable performance. After quick recovery,its effluent total nitrogen was below 15 mg / L. When shocked by 0% salinity,the control system was failed and unable to restore. While the bioaugmentation system recovered quickly by inoculating 3% of salt-tolerant denitrification composite microbial inoculum,and total nitrogen of effluent dropped to below 15 mg / L again. This research could provide technical support for construction and operation of bioaugmentation saline wastewater denitrification system.
引文
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[8]Xie Daohai,Li Chenchen,Tang Rong,et al.Ion-exchange membrane bioelectrochemical reactor for removal of nitrate in the biological effluent from a coking wastewater treatment plant.Electrochemistry Communications,2014,46:99-102
[9]张智,周健.食品工业高盐高氮磷有机废水处理技术发展趋势.给水排水,2012,38(9):1,51Zhang Zhi,Zhou Jian.Technology development trend of organic food industry wastewater with high salinity and high nitrogen and phosphorus.Water&Wastewater Engineering,2012,38(9):1,51(in Chinese)
[10]Stephenson D.,Stephenson T.Bioaugmentation for enhancing biological wastewater treatment.Biotechnology Advances,1992,10(4):549-559
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[12]张雨山,王静,任华峰,等.投加有效微生物强化CAST反应器处理含盐废水.中国给水排水,2012,28(1):81-83Zhang Yushan,Wang Jing,Ren Huafeng,et al.Enhanced treatment of salinity wastewater in CAST reactor with effective microbes.China Water&Wastewater,2012,28(1):81-83(in Chinese)
[13]Lefebvre O.,Habouzit F.,Bru V.,et al.Treatment of hypersaline industrial wastewater by a microbial consortium in a sequencing batch reactor.Environmental Technology,2004,25(5):543-553
[14]Choi M.H.,Park Y.H.Growth of Pichia guilliermondii A9,an osmotolerant yeast,in waste brine generated from kimchi production.Bioresource Technology,1999,70(3):231-236
[15]唐婧,陈金楠,肖亚男,等.不同条件下耐盐脱氮复合菌剂的脱氮特性.沈阳建筑大学学报(自然科学版),2013,29(6):1133-1139Tang Jing,Chen Jingnan,Xiao Yanan,et al.Denitrification characteristics of halophilic denitrification complex microbial inoculants under different conditions.Journal of Shenyang Jianzhu University(Natural Science),2013,29(6):1133-1139(in Chinese)
[16]曲洋,张培玉,于德爽,等.异养硝化/好氧反硝化菌生物强化含海水污水的SBR短程硝化系统初探.环境科学,2010,31(10):2376-2384Qu Yang,Zhang Peiyu,Yu Deshuang,et al.Bioaugmentation for shortcut nitrification in SBR treating for sewage containing Sea water by nitrification-aerobic denitrification bacteria.Environmental Science,2010,31(10):2376-2384(in Chinese)
[17]陈金楠.耐盐脱氮复合菌剂的构建及其生物强化效果.沈阳:沈阳建筑大学硕士学位论文,2013Chen Jinnan.Research on halophilic denitrification complex microbial inoculants and their bioaugmentation effects.Shenyang:Master Dissertation of Shenyang Jianzhu University,2013(in Chinese)
[18]崔丽.一种复合菌剂的构建及其处理生活污水的研究.沈阳:东北大学博士学位论文,2008Cui Li.On building-up a synthetic microbe agent and its treating of domestic sewage.Shenyang:Doctoral Dissertation of Northeastern University,2008(in Chinese)
[19]孙雪梅,李秋芬,张艳,等.一株海水异养硝化-好氧反硝化菌系统发育及脱氮特性.微生物学报,2012,52(6):687-695Sun Xuemei,Li Qiufen,Zhang Yang,et al.Phylogenetic analysis and nitrogen removal characteristics of aheterotrophic nitrifying-aerobic denitrifying bacteria strain from marine environment.Acta Microbiologica Sinica,2012,52(6):687-695(in Chinese)
[2]朱庆平,史晓明,詹红丽,等.我国海水利用现状、问题及发展对策研究.中国水利,2012,(21):30-33Zhu Qingping,Shi Xiaoming,Zhan Hongli,et al.Seawater utilization in China:current situation,issue and development strategy.China Water Resources,2012,(21):30-33(in Chinese)
[3]林于廉,张钊,莊瑞鑫,等.两相分配式生物反应器处理高盐废水中的苯酚.环境工程学报,2013,7(3):1029-1034Lin Yulian,Zhang Zhao,Zhuang Ruixin,et al.Treatment of phenol in saline solutions by a novel two-phase partitioning bioreactor.Chinese Journal of Environmental Engineering,2013,7(3):1029-1034(in Chinese)
[4]张永胜,周健,韩懿,等.ASBBR反应器处理超高盐度榨菜腌制废水的效能.中国给水排水,2013,29(1):18-21Zhang Yongsheng,Zhou Jian,Han Yi,et al.Efficiency of anaerobic sequencing batch biofilm reactor for treatment of hypersaline mustard tuber wastewater.China Water&Wastewater,2013,29(1):18-21(in Chinese)
[5]任南琪,周显娇,郭婉茜,等.染料废水处理技术研究进展.化工学报,2013,64(1):84-94Ren Nanqi,Zhou Xianjiao,Guo Wanqian,et al.A review on treatment methods of dye wastewater.CIESC Journal,2013,64(1):84-94(in Chinese)
[6]Pendashteh A.R.,Abdullah L.C.,Fakhru’l-Razi A.,et al.Evaluation of membrane bioreactor for hypersaline oily wastewater treatment.Process Safety and Environmental Protection,2012,90(1):45-55
[7]赵俊.高盐高氮有机废水与城镇污水协同处理实验研究.重庆:重庆大学硕士学位论文,2011Zhao Jun.Study on co-processing municipal sewage and organic wastewater with high concentration of salt and nitrogen.Chongqing:Master Dissertation of Chongqing University,2011(in Chinese)
[8]Xie Daohai,Li Chenchen,Tang Rong,et al.Ion-exchange membrane bioelectrochemical reactor for removal of nitrate in the biological effluent from a coking wastewater treatment plant.Electrochemistry Communications,2014,46:99-102
[9]张智,周健.食品工业高盐高氮磷有机废水处理技术发展趋势.给水排水,2012,38(9):1,51Zhang Zhi,Zhou Jian.Technology development trend of organic food industry wastewater with high salinity and high nitrogen and phosphorus.Water&Wastewater Engineering,2012,38(9):1,51(in Chinese)
[10]Stephenson D.,Stephenson T.Bioaugmentation for enhancing biological wastewater treatment.Biotechnology Advances,1992,10(4):549-559
[11]王建芳,赵庆良,林佶侃,等.生物强化技术及其在废水生物处理中的应用.环境工程学报,2007,1(9):40-45Wang Jianfang,Zhao Qingliang,Lin Jikan,et al.Bioaugmentation technology and its application in biological wastewater treatment.Chinese Journal of Environmental Engineering,2007,1(9):40-45(in Chinese)
[12]张雨山,王静,任华峰,等.投加有效微生物强化CAST反应器处理含盐废水.中国给水排水,2012,28(1):81-83Zhang Yushan,Wang Jing,Ren Huafeng,et al.Enhanced treatment of salinity wastewater in CAST reactor with effective microbes.China Water&Wastewater,2012,28(1):81-83(in Chinese)
[13]Lefebvre O.,Habouzit F.,Bru V.,et al.Treatment of hypersaline industrial wastewater by a microbial consortium in a sequencing batch reactor.Environmental Technology,2004,25(5):543-553
[14]Choi M.H.,Park Y.H.Growth of Pichia guilliermondii A9,an osmotolerant yeast,in waste brine generated from kimchi production.Bioresource Technology,1999,70(3):231-236
[15]唐婧,陈金楠,肖亚男,等.不同条件下耐盐脱氮复合菌剂的脱氮特性.沈阳建筑大学学报(自然科学版),2013,29(6):1133-1139Tang Jing,Chen Jingnan,Xiao Yanan,et al.Denitrification characteristics of halophilic denitrification complex microbial inoculants under different conditions.Journal of Shenyang Jianzhu University(Natural Science),2013,29(6):1133-1139(in Chinese)
[16]曲洋,张培玉,于德爽,等.异养硝化/好氧反硝化菌生物强化含海水污水的SBR短程硝化系统初探.环境科学,2010,31(10):2376-2384Qu Yang,Zhang Peiyu,Yu Deshuang,et al.Bioaugmentation for shortcut nitrification in SBR treating for sewage containing Sea water by nitrification-aerobic denitrification bacteria.Environmental Science,2010,31(10):2376-2384(in Chinese)
[17]陈金楠.耐盐脱氮复合菌剂的构建及其生物强化效果.沈阳:沈阳建筑大学硕士学位论文,2013Chen Jinnan.Research on halophilic denitrification complex microbial inoculants and their bioaugmentation effects.Shenyang:Master Dissertation of Shenyang Jianzhu University,2013(in Chinese)
[18]崔丽.一种复合菌剂的构建及其处理生活污水的研究.沈阳:东北大学博士学位论文,2008Cui Li.On building-up a synthetic microbe agent and its treating of domestic sewage.Shenyang:Doctoral Dissertation of Northeastern University,2008(in Chinese)
[19]孙雪梅,李秋芬,张艳,等.一株海水异养硝化-好氧反硝化菌系统发育及脱氮特性.微生物学报,2012,52(6):687-695Sun Xuemei,Li Qiufen,Zhang Yang,et al.Phylogenetic analysis and nitrogen removal characteristics of aheterotrophic nitrifying-aerobic denitrifying bacteria strain from marine environment.Acta Microbiologica Sinica,2012,52(6):687-695(in Chinese)