不同种类浒苔多糖的体外抗菌活性研究
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摘要
为研究浒苔多糖粗提物、浒苔多糖硒的抑菌活性,本试验以硫酸黏杆菌素、林可霉素、黄芪多糖和酵母细胞壁多糖为对照品,采用药敏试验检测浒苔多糖粗提物、浒苔多糖硒对大肠杆菌、沙门氏菌和金黄色葡萄球菌的抑菌活性。结果表明:10～100 mg/mL浓度的浒苔多糖粗提物对大肠杆菌、沙门氏菌、金黄色葡萄球菌均未产生抑菌作用,浓度为20、50、100 mg/mL的浒苔多糖硒对金黄色葡萄球菌的抑菌圈和抑菌活性分别为6 mm(-)、8 mm(+)、18 mm(++),对大肠杆菌、沙门氏菌未产生抑菌作用。
The purpose of this experiment was to investigate the antibacterial activity of extract of polysaccharide from Brassica oleracea and polysaccharide Se of Brassica.Using colistin sulfate,lincomycin,astragalus polysaccharide and yeast cell wall polysaccharide as the reference substance,the susceptibility test was used to detect the antibacterial activity of the crude polysaccharide extract of caninus sphagnum polysaccharide and canola polysaccharide against Escherichia coli,Salmonella and Staphylococcus aureus.active.The results showed that 10 ～ 100 mg/mL of crude polysaccharide had no antibacterial activity against Escherichia coli,Salmonella and Staphylococcus aureus.The concentration of 20,50,100 mg/mL of enteromorpha polysaccharides selenium Staphylococcus aureus inhibition zone and antibacterial activity were 6 mm(-),8 mm(+),18 mm(++),no antibacterial effect on E.coli and Salmonella.
The purpose of this experiment was to investigate the antibacterial activity of extract of polysaccharide from Brassica oleracea and polysaccharide Se of Brassica.Using colistin sulfate,lincomycin,astragalus polysaccharide and yeast cell wall polysaccharide as the reference substance,the susceptibility test was used to detect the antibacterial activity of the crude polysaccharide extract of caninus sphagnum polysaccharide and canola polysaccharide against Escherichia coli,Salmonella and Staphylococcus aureus.active.The results showed that 10 ～ 100 mg/mL of crude polysaccharide had no antibacterial activity against Escherichia coli,Salmonella and Staphylococcus aureus.The concentration of 20,50,100 mg/mL of enteromorpha polysaccharides selenium Staphylococcus aureus inhibition zone and antibacterial activity were 6 mm(-),8 mm(+),18 mm(++),no antibacterial effect on E.coli and Salmonella.
引文
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[6]路志芳,蒋鹏飞.香菇多糖的抑菌效果试验[J].上海蔬菜,2017,4:85~87.
[7]路则庆.Enterobacter cloacae Z0206富硒多糖对动物生长性能和免疫功能的影响及其作用机制研究:[博士学位论文][D].杭州:浙江大学,2014.
[8]Mehrtens G.Haloperoxidase activities in Arctic macroalgae[J].Polar Biology,1994,14:351~354.
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[10]Rizvi M A,Farooqui S,Shameel M.Bioactivity and elemental composition of certain seaweeds from Karachi coast[J].Pakistan Journal of Marine Biology,2000,6(2):207~218.
[2]高玉杰,吕海涛.浒苔多糖和硒化浒苔多糖抑菌作用研究[J].食品科技,2013,1(38):195~205.
[3]高玉杰,吕海涛.浒苔硒多糖的制备及其活性研究[J].化学与生物工程,2012,29(5):40~45.
[4]胡桂学,陈金顶,彭远义,等.兽医微生物学试验教程[M].北京:中国农业大学出版社,2015.
[5]韩玲,张淑平,刘晓慧.海藻生物活性物质应用研究进展[J].化工进展,2012,8:1794~1800.
[6]路志芳,蒋鹏飞.香菇多糖的抑菌效果试验[J].上海蔬菜,2017,4:85~87.
[7]路则庆.Enterobacter cloacae Z0206富硒多糖对动物生长性能和免疫功能的影响及其作用机制研究:[博士学位论文][D].杭州:浙江大学,2014.
[8]Mehrtens G.Haloperoxidase activities in Arctic macroalgae[J].Polar Biology,1994,14:351~354.
[9]Vlachos V,Critchley A T.Antimicrobial activity of extracts from selected southern African marine macroalgae[J].South African Journal of Science,1997,93(7):328~332.
[10]Rizvi M A,Farooqui S,Shameel M.Bioactivity and elemental composition of certain seaweeds from Karachi coast[J].Pakistan Journal of Marine Biology,2000,6(2):207~218.