大孔树脂固定化黑豆过氧化物酶的吸附行为研究
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摘要
将从陕北小粒黑豆中提取的过氧化物酶经过分离纯化后,用大孔树脂吸附法对其进行固定化研究,用吸附性能较好的LXD-762型树脂对其进行吸附动力学和吸附热力学研究。结果表明,该吸附过程符合Langmuir吸附模型,且吸附过程是自发进行的吸热过程,其吸附速率符合准二级动力学方程。
Peroxidase was extracted, isolated, purificated from black beans in north of Shaanxi Province which was immobilizated by macroporous resin. The adsorption kinetics and thermodynamics of peroxidase on the LXD-762 resin were studied. The results showed that the adsorption data for peroxidase was accorded with Langmuir model, the adsorption was spontaneous and endothermic. The adsorption rate of peroxidase was fit for Pseudo-second-order kinetics equation.
Peroxidase was extracted, isolated, purificated from black beans in north of Shaanxi Province which was immobilizated by macroporous resin. The adsorption kinetics and thermodynamics of peroxidase on the LXD-762 resin were studied. The results showed that the adsorption data for peroxidase was accorded with Langmuir model, the adsorption was spontaneous and endothermic. The adsorption rate of peroxidase was fit for Pseudo-second-order kinetics equation.
引文
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[2]韩莉,陶菡,张义明.辣根过氧化物酶/聚乙烯醇缩丁醛/碳纳米管修饰玻碳电极检测过氧化氢.中国食品添加剂[J].2010,(2):225-228.
[3]展海军,马超越,白静.固定化辣根过氧化物酶生物传感器测定火腿肠中亚硝酸盐.食品研究与开发[J].2011,32(2):123-125.
[4]温研,赵新淮.牛乳的过氧化物酶处理与凝固型酸奶品质的变化.食品与发酵工业[J].2011,32(2):41-45.
[5]Li H M,Nicell J A.Optimal conditions for oxidative degradation of bisphenol A by Horseradish Peroxidase in aqueous phase.Multimedia Technology[J].2011,26~28:5442-5446.
[6]鲍腾,彭书传,陈冬,等.凹凸棒石粘土固定辣根过氧化物酶处理含酚废水.环境工程学报[J].2012,6(9):3179-3185.
[7]罗贵民.酶工程[M].北京:化学工业出版社,2003,p56.
[8]任萍,王齐伟,武彩露,等.大孔吸附树脂对罗布麻叶中总黄酮的纯化研究.离子交换与吸附[J].2016,32(2):141-153.
[9]王华,王莹,詹长娟,等.脂肪酶固定化工艺优化及其酶学性质研究.食品研究与开发[J].2015,36(21):118-123.
[10]陈姗姗,李党生.以离子交换树脂为载体的果胶酶固定化研究.江苏农业科学[J].2013,41(4):251-253.
[11]张媛,康龙丽,金天博,等.西藏雪莲总黄酮在大孔吸附树脂上的吸附动力学和热力学研究.离子交换与吸附[J].2016,32(4):358-367.
[12]李忠光,龚明.愈创木酚法测定植物过氧化物酶活性的改进.植物生理学通讯[J].2008,44(2):323-324.