基于电诱导沉积方法制备海藻酸钙水凝胶
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摘要
海藻酸钙水凝胶是一种具有良好生物相容性、生物降解性的生物医用高分子材料,但是传统制备方法不能得到具有生理结构的水凝胶。文中提出了一种基于电诱导沉积原理,使用海藻酸钠和碳酸钙混合溶液制备海藻酸钙水凝胶的方法。在分析电诱导沉积原理基础上,搭建了海藻酸钙水凝胶3D打印系统。建立了海藻酸钙水凝胶截面宽度模型,提出了通过调节打印喷头的移动速度控制凝胶截面宽度的方法。应用该系统打印了圆管形、正六边形及四叶草形的生物支架,并用这些结构的生物支架进行细胞培养实验,获得具有一定生物活性的细胞。
Calcium alginate hydrogel is a kind of well biocompatible and biodegradable biomedical polymer material.However,traditional preparation methods cannot obtain hydrogel with physical structure. This paper proposed a method which made use of mixed solution of sodium alginate and calcium carbonate as raw material for the preparation of calcium alginate hydrogel based on the principle of electrically induced deposition. On the basis of analyzing electrically induced deposition. This paper built a calcium alginate hydrogel 3D printing system,established a model of cross-sectional width for calcium alginate hydrogel,and put forward the method of controlling the gel-sectional width by adjusting the speed of the print head. This paper also adopted the system to print pipe shaped,hexagonal and clover-shaped bio-scaffold structure which can be used for cell culture experiments in which biological activated cell can be obtained.
Calcium alginate hydrogel is a kind of well biocompatible and biodegradable biomedical polymer material.However,traditional preparation methods cannot obtain hydrogel with physical structure. This paper proposed a method which made use of mixed solution of sodium alginate and calcium carbonate as raw material for the preparation of calcium alginate hydrogel based on the principle of electrically induced deposition. On the basis of analyzing electrically induced deposition. This paper built a calcium alginate hydrogel 3D printing system,established a model of cross-sectional width for calcium alginate hydrogel,and put forward the method of controlling the gel-sectional width by adjusting the speed of the print head. This paper also adopted the system to print pipe shaped,hexagonal and clover-shaped bio-scaffold structure which can be used for cell culture experiments in which biological activated cell can be obtained.
引文
[1]Subia B,Kundu J.Biomaterial scaffold fabrication techniques for potential tissue engineering applications[J].Tissue Eng.,2010,12:142-157.
[2]葛建华,张玉军,李木森,等.溶胶凝胶法制备含生物成分的PLGA组织工程支架材料[J].高分子材料科学与工程,2011,27(10):169-176.Ge J H,Zhang Y J,Li M S,et al.Research of bioactive PLGA tissue engineering materials processed by sol-gel method[J].Polymer Materials Science&Engineering,2011,27(10):169-176.
[3]谭帼馨,王迎军,宁成云,等.组织工程用PEGDA水凝胶材料低温等离子体接枝聚合[J].高分子材料科学与工程,2009,25(12):13-15.Tan G X,Wang Y J,Ning C Y,et al.Low temperature plasma treatment for the PEGDA-based hydrogels[J].Polymer Materials Science&Engineering,2009,25(12):13-15.
[4]石静,钟玉敏.组织工程中3D生物打印技术的应用[J].中国组织工程研究,2014,18(2):271-276.Shi J,Zhong Y M.Three-dimensional bioprinting technology in tissue engineering[J].Chinese Journal of Tissue Engineering Research,2014,18(2):271-276.
[5]Goekbora B,Pomerantz C,Premnath N,et al.A hydrogel wound dressing with gradient cross linking and silver/copper ions for treatment of severe burns[C].Annual Northeast Bioengineering Conference,2013:237-238.
[6]Fotticchia A,Liu Y,Demirci E,et al.Electrospun polycaprolactone nano-fibers support growth of human mesenchymal stem cells[C].Proceedings of the 13th IEEE International Conference on Nanotechnology,2013:158-161.
[7]高春梅,柳明珠,吕少瑜,等.海藻酸钠水凝胶的制备及其在药物释放中的应用[J].化学进展,2013,25(6):1012-1022.Gao C M,Liu M Z,LüS Y,et al.Preparation of sodium alginate hydrogel and its application in drug release[J].Progress in Chemistry,2013,25(6):1012-1022.
[8]Shi X W,Tsao C Y,Yang X,et al.Electro addressing of cell populations by co-deposition with calcium alginate hydrogels[J].Adv.Funct.Mater.,2009,19:2074-2080.
[9]Cheng Y,Luo X L,Chen Y,et al.Biocompatible multi-address 3D cell assembly in microfluidic devices using spatially programmable gel formation[EB//OL].http://d.g.wanfangdata.com.cn/NSTLQK_NSTL_QKJJ0222094252.aspx.
[10]Ozawa F,Ino K,Takahashi Y,et al.Electrodeposition of alginate gels for construction of vascular-like structures[J].J.Biosci.Bioeng.,2013,115:459-461.
[11]Betz J F,Cheng Y,Tsao C Y,et al.Optically clear alginate hydrogels for spatially controlled cell entrapment and culture at microfluidic electrode surfaces[J].Lab on A Chip,2013,13:1854-1858.
[2]葛建华,张玉军,李木森,等.溶胶凝胶法制备含生物成分的PLGA组织工程支架材料[J].高分子材料科学与工程,2011,27(10):169-176.Ge J H,Zhang Y J,Li M S,et al.Research of bioactive PLGA tissue engineering materials processed by sol-gel method[J].Polymer Materials Science&Engineering,2011,27(10):169-176.
[3]谭帼馨,王迎军,宁成云,等.组织工程用PEGDA水凝胶材料低温等离子体接枝聚合[J].高分子材料科学与工程,2009,25(12):13-15.Tan G X,Wang Y J,Ning C Y,et al.Low temperature plasma treatment for the PEGDA-based hydrogels[J].Polymer Materials Science&Engineering,2009,25(12):13-15.
[4]石静,钟玉敏.组织工程中3D生物打印技术的应用[J].中国组织工程研究,2014,18(2):271-276.Shi J,Zhong Y M.Three-dimensional bioprinting technology in tissue engineering[J].Chinese Journal of Tissue Engineering Research,2014,18(2):271-276.
[5]Goekbora B,Pomerantz C,Premnath N,et al.A hydrogel wound dressing with gradient cross linking and silver/copper ions for treatment of severe burns[C].Annual Northeast Bioengineering Conference,2013:237-238.
[6]Fotticchia A,Liu Y,Demirci E,et al.Electrospun polycaprolactone nano-fibers support growth of human mesenchymal stem cells[C].Proceedings of the 13th IEEE International Conference on Nanotechnology,2013:158-161.
[7]高春梅,柳明珠,吕少瑜,等.海藻酸钠水凝胶的制备及其在药物释放中的应用[J].化学进展,2013,25(6):1012-1022.Gao C M,Liu M Z,LüS Y,et al.Preparation of sodium alginate hydrogel and its application in drug release[J].Progress in Chemistry,2013,25(6):1012-1022.
[8]Shi X W,Tsao C Y,Yang X,et al.Electro addressing of cell populations by co-deposition with calcium alginate hydrogels[J].Adv.Funct.Mater.,2009,19:2074-2080.
[9]Cheng Y,Luo X L,Chen Y,et al.Biocompatible multi-address 3D cell assembly in microfluidic devices using spatially programmable gel formation[EB//OL].http://d.g.wanfangdata.com.cn/NSTLQK_NSTL_QKJJ0222094252.aspx.
[10]Ozawa F,Ino K,Takahashi Y,et al.Electrodeposition of alginate gels for construction of vascular-like structures[J].J.Biosci.Bioeng.,2013,115:459-461.
[11]Betz J F,Cheng Y,Tsao C Y,et al.Optically clear alginate hydrogels for spatially controlled cell entrapment and culture at microfluidic electrode surfaces[J].Lab on A Chip,2013,13:1854-1858.