龙牡桂枝汤对多动症模型大鼠左右前额叶皮质和纹状体BDNF及GDNF mRNA的影响
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摘要
目的:观察龙牡桂枝汤对多动症模型大鼠左右前额叶皮质和纹状体脑源性神经营养因子(BDNF)、胶质细胞源性神经营养因子(GDNF) mRNA表达的影响。方法:幼年雄性SHR大鼠随机分为龙牡桂枝汤组、盐酸哌甲酯组和生理盐水组,分别予相应药物灌胃14d,采用实时荧光定量PCR法检测大鼠左侧前额叶皮质和纹状体BDNF、GDNF mRNA的表达水平。结果:组内比较:生理盐水组SHR大鼠左侧前额叶皮质BDNF和GDNF mRNA表达较右侧显著升高(P<0.05),而左侧纹状体BDNF和GDNF mRNA表达较右侧显著降低(P<0.05)。组间比较:两用药组左侧前额叶皮质BDNF mRNA表达较生理盐水组显著降低(P<0.01),两用药组右侧纹状体BDNF mRNA表达较生理盐水组显著升高(P<0.05)。龙牡桂枝汤组左纹状体GDNF mRNA表达较生理盐水组显著升高(P<0.05)。结论:SHR大鼠左右前额叶皮质和纹状体BDNF、GDNF mRNA表达存在差异,龙牡桂枝汤治疗多动症的作用机制可能与调节左右前额叶皮质和纹状体BDNF mRNA及左侧纹状体GDNF mRNA表达有关。
Objective: To study effects of Longmu Guizhi Decoction on the mRNA expression of BDNF and GDNF in the left and right prefrontal cortex and striatum of spontaneously hypertensive rats(SHR) with hyperactivity disorder. Methods:Juvenile male SHR rats were randomly divided into Longmu Guizhi Decoction group(LD), methylphenidate control group and saline control group. The rats received gastric gavage of corresponding drugs for 14 days. The Real-time PCR method was used to test the BDNF and GDNF mRNA expressions in the left and right prefrontal cortexand striatum. Results: Self comparison:in saline control group, BDNF and GDNF mRNA expression of the left prefrontal cortex were significantly higher than the right(P<0.05), but the expression of BDNF and GDNF mRNA in the left striatum was lower than that in the right(P<0.05),Comparison among groups: BDNF mRNA expression in the left prefrontal cortex of methylphenidate control group and LD group were significantly lower than the saline control group(P<0.01), BDNF mRNA expression in the right striatum of methylphenidate control group and LD group were significantly higher than the saline control group(P<0.05), GDNF mRNA expression in the left striatum of LD group were significantly higher than the saline control group(P<0.05). Conclusion: BDNF and GDNF mRNA expression of SHR rats were different in the left and right prefrontal cortex and striatum. The potential mechanism of LD in the treatment of ADHD is related to the regulation of the BDNF mRNA expression in the left and right prefrontal cortex and striatum,as well as GDNF mRNA expression in the left striatum.
Objective: To study effects of Longmu Guizhi Decoction on the mRNA expression of BDNF and GDNF in the left and right prefrontal cortex and striatum of spontaneously hypertensive rats(SHR) with hyperactivity disorder. Methods:Juvenile male SHR rats were randomly divided into Longmu Guizhi Decoction group(LD), methylphenidate control group and saline control group. The rats received gastric gavage of corresponding drugs for 14 days. The Real-time PCR method was used to test the BDNF and GDNF mRNA expressions in the left and right prefrontal cortexand striatum. Results: Self comparison:in saline control group, BDNF and GDNF mRNA expression of the left prefrontal cortex were significantly higher than the right(P<0.05), but the expression of BDNF and GDNF mRNA in the left striatum was lower than that in the right(P<0.05),Comparison among groups: BDNF mRNA expression in the left prefrontal cortex of methylphenidate control group and LD group were significantly lower than the saline control group(P<0.01), BDNF mRNA expression in the right striatum of methylphenidate control group and LD group were significantly higher than the saline control group(P<0.05), GDNF mRNA expression in the left striatum of LD group were significantly higher than the saline control group(P<0.05). Conclusion: BDNF and GDNF mRNA expression of SHR rats were different in the left and right prefrontal cortex and striatum. The potential mechanism of LD in the treatment of ADHD is related to the regulation of the BDNF mRNA expression in the left and right prefrontal cortex and striatum,as well as GDNF mRNA expression in the left striatum.
引文
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[11]Lyons W E,Mamounas L A,Ricaurte G A,et al.Brain derived neurotrophic factor-deficient mice develop aggressiveness and hyperphagia in conjunction with brain serotonergic abnormalities.Proc Natl Acad Sci USA,1999,96(26):15239-15244
[12]Reis R A,Cabral de Silra M C,Loureiro dos Santos N E,et al.Sympathetic neuronal survival induced by retinal trophic factors.J Neurobiol,2002,50(1):13-23
[13]Inoue K.The mechanism and control of neuropathic pain.Rinsho Shinkeigaku,2009,49(11):779-782
[14]胡颖,林忠东,郑飞霞,等.人参皂苷Rg1对SHR大鼠纹状体GDNF mRNA基因表达和多巴胺含量的影响.中华中医药学刊,2012,30(4):862-864
[15]刘琪,陈丽舟,李飞,等.基于体素的形态学方法对注意缺陷多动障碍男性患儿的脑灰质体积分析.生物医学工程学杂志,2016,33(3):526-532
[16]顾国祥,韩新民,孙继超,等.儿童多动症中医治疗思路探讨.中华中医药杂志,2018,33(8):3481-3482
[2]翟杰,张华东,徐淑祝,等.注意缺陷多动障碍伴学习障碍患儿血清BDNF水平研究.山东医药,2007,47(4)54-55
[3]胡颖,林忠东,郑飞霞.哌甲酯控释剂对自发性高血压大鼠纹状体胶质细胞源性神经营养因子m RNA表达的作用.实用医学杂志,2011,27(24):4383-4385
[4]Frodl T,Skokauskas N.Meta-analysis of structural MRI studies in children and adults with attention deficit hyperactivity disorder indicates treatment effects.Acta Psychiatr Scand,2011,125(2):114-126
[5]郭润蒲,杜亚松.注意缺陷多动障碍相关脑网络结构及功能磁共振研究进展.中国神经精神疾病杂志,2016,42(9):556-559
[6]何平,朱瑛.刘以敏老师辨证治疗儿童多动症经验初探.云南中医中药杂志,2006,27(1):5
[7]何平,唐彦,杨若俊.龙牡桂枝汤改善注意缺陷多动障碍大鼠行为的实验研究.中国中西医结合儿科学,2014,6(6):203-205
[8]郑小兰,陈燕惠.注意缺陷多动障碍的实验动物模型.中华行为医学与脑科学杂志,2015,24(3):276-279
[9]Andersen S L.Changes in the second messenger cyclic AMP during development may underlie motoric symptoms in attention deficit/hyperactivity disorder(ADHD).Behav Brain Res,2002,130(1-2):197-201
[10]Benmansour S,Deltheil T,Piotrowski J.Influence of brain-derived neurotrophic factor(BDNF)on serotonin neurotransmission in the hippocampus of adult rodents.Eur J Pharmaco,2008,587(1-3):90-98
[11]Lyons W E,Mamounas L A,Ricaurte G A,et al.Brain derived neurotrophic factor-deficient mice develop aggressiveness and hyperphagia in conjunction with brain serotonergic abnormalities.Proc Natl Acad Sci USA,1999,96(26):15239-15244
[12]Reis R A,Cabral de Silra M C,Loureiro dos Santos N E,et al.Sympathetic neuronal survival induced by retinal trophic factors.J Neurobiol,2002,50(1):13-23
[13]Inoue K.The mechanism and control of neuropathic pain.Rinsho Shinkeigaku,2009,49(11):779-782
[14]胡颖,林忠东,郑飞霞,等.人参皂苷Rg1对SHR大鼠纹状体GDNF mRNA基因表达和多巴胺含量的影响.中华中医药学刊,2012,30(4):862-864
[15]刘琪,陈丽舟,李飞,等.基于体素的形态学方法对注意缺陷多动障碍男性患儿的脑灰质体积分析.生物医学工程学杂志,2016,33(3):526-532
[16]顾国祥,韩新民,孙继超,等.儿童多动症中医治疗思路探讨.中华中医药杂志,2018,33(8):3481-3482