大黄?虫丸对博来霉素致肺纤维化模型大鼠胶原蛋白和肺组织羟脯氨酸的影响
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摘要
目的基于"肺系络病"理论,研究搜剔肺络法代表方大黄虫丸对博来霉素致肺纤维化模型大鼠胶原蛋白含量和肺组织羟脯氨酸(hydroxyproline,HYP)的影响。方法选择雄性SD大鼠60只,随机分为6组即空白组、模型组、醋酸泼尼松组、大黄虫丸低剂量组、大黄虫丸中剂量组、大黄虫丸高剂量组,每组10只。于造模28天后常规每日一次灌胃给药,给药30天后麻醉取肺组织,进行HE、Masson染色,肺组织胶原蛋白含量测定,肺组织HYP含量测定,并比较各指标的组间差异。结果胶原含量的高低方面,与模型组、醋酸泼尼松组相比,大黄虫丸各剂量组表达明显减弱(P<0.05);在HYP含量测定方面,模型组及各药物干预组明显高于空白组(P<0.05),模型组与醋酸泼尼松组及大黄虫丸各剂量组两两之间无明显差异(P>0.05)。本研究说明大黄虫丸对肺纤维化模型大鼠具有一定的治疗作用,并优于西药激素的治疗效果,且其治疗作用是通过对胶原蛋白的表达抑制实现的,其并不能抑制肺组织HYP的产生。结论大黄虫丸可能通过抑制胶原蛋白的表达发挥抗纤维化的作用。
Objective To study the effects of Dahuang Zhechong pills as a representative prescription of picking the lung collaterals method on collagen content and hydroxyproline( HYP) in lung tissue of rats with bleomycin-induced pulmonary fibrosis based on the theory of collateral disease in the lung.Methods A total of 60 SD male rats were divided into the blank group,model group,Prednisone Acetate group,Dahuang Zhechong pills small dose group,middle does group,and large does group,with 10 rats in each group. After 28 days of establishing models,the rats were given drugs respectively by intragastric administration once a day. In 30 days after anesthesia,the lung tissues were harvested for HE,Masson staining,and collagen content and content of HYP in lung tissue were detected. The differences in index of groups were compared. Results In terms of collagen content,compared with model group,the prednisone acetate group,Dahuang Zhechong pills groups decreased significantly( P < 0. 05). In terms of HYP content determination,model group and the drug intervention groups were significantly higher than that of blank group( P < 0. 05). There were no significant differences between the model group,prednisone acetate group and Dahuang Zhechong pills groups( P > 0. 05). This study indicated that Dahuang Zhechong pills had certain therapeutic effects,and was superior to western medicine hormone on the treatment effect,which was realized by inhibiting the collagen content,but it could not inhibit HYP in lung tissue. Conclusion Dahuang Zhechong pills plays the role of anti-fibrosis by inhibiting the collagen content.
Objective To study the effects of Dahuang Zhechong pills as a representative prescription of picking the lung collaterals method on collagen content and hydroxyproline( HYP) in lung tissue of rats with bleomycin-induced pulmonary fibrosis based on the theory of collateral disease in the lung.Methods A total of 60 SD male rats were divided into the blank group,model group,Prednisone Acetate group,Dahuang Zhechong pills small dose group,middle does group,and large does group,with 10 rats in each group. After 28 days of establishing models,the rats were given drugs respectively by intragastric administration once a day. In 30 days after anesthesia,the lung tissues were harvested for HE,Masson staining,and collagen content and content of HYP in lung tissue were detected. The differences in index of groups were compared. Results In terms of collagen content,compared with model group,the prednisone acetate group,Dahuang Zhechong pills groups decreased significantly( P < 0. 05). In terms of HYP content determination,model group and the drug intervention groups were significantly higher than that of blank group( P < 0. 05). There were no significant differences between the model group,prednisone acetate group and Dahuang Zhechong pills groups( P > 0. 05). This study indicated that Dahuang Zhechong pills had certain therapeutic effects,and was superior to western medicine hormone on the treatment effect,which was realized by inhibiting the collagen content,but it could not inhibit HYP in lung tissue. Conclusion Dahuang Zhechong pills plays the role of anti-fibrosis by inhibiting the collagen content.
引文
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[2]张立山,武维屏.干血与肺间质纤维化[J].中国中医药信息杂志,2012,19(12):96-97.
[3]张立山,戴雁彦,任传云.武维屏教授治疗肺纤维化十法[J].中国中医药信息杂志,2008,15(4):94.
[4]张天嵩,韩镭.吴银根教授治疗肺纤维化用药特色浅析[J].中医药学刊,2003,21(3):346-347.
[5]杨丽,吕晓东,刘勇明,等.中医药防治肺纤维化氧化应激作用机制研究进展[J].世界中西医结合杂志,2017,12(3):298-299.