HPLC法测定杜仲平压片中槲皮素的含量
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摘要
目的建立采用高效液相色谱法(HPLC)测定杜仲平压片中槲皮素的含量。方法色谱柱为Thermo Hypersil Gold-C18(4.6 mm 250 mm,5μm),流动相为乙腈-0.4磷酸溶液(27∶73),流速1.0 mL·min ~(-1),柱温35℃,检测波长为256 nm。结果槲皮素在3.21～45.02μg·mL~(-1)范围内线性关系良好(R~2=0.9999),平均回收率为96.83%(RSD=1.11%)。结论所建立的方法结果准确度高,专属性强,重现性好,可用于测定杜仲平压片中槲皮素的含量。
Objective To establish High Performance Liquid Chromatography(HPLC) method to determine quercetin in Duzhong pingya Tablet. Methods The chromatographic column was Thermo Hypersil Gold-C18(4.6 mm×250 mm,5 μm),and the mobile phase was consisted of acetonitrile and 0.5% phosphoric acid(27∶73).The flow rate was 1.0 mL·min~(-1). The column temperature was set at 35 ℃, and the detection wavelengths were 256 nm. Results The linearity of chlorogenic quercetin was good in the range of 3.21～45.02 μg·mL~(-1)(R~2=0.9999)and the average recoveries was 96.83%(RSD=1.11%)for quercetin. Conclusion The established method is accurate and reproducible,and can be applied for the determination of quercetin in Duzhong Pingya Tablet.
Objective To establish High Performance Liquid Chromatography(HPLC) method to determine quercetin in Duzhong pingya Tablet. Methods The chromatographic column was Thermo Hypersil Gold-C18(4.6 mm×250 mm,5 μm),and the mobile phase was consisted of acetonitrile and 0.5% phosphoric acid(27∶73).The flow rate was 1.0 mL·min~(-1). The column temperature was set at 35 ℃, and the detection wavelengths were 256 nm. Results The linearity of chlorogenic quercetin was good in the range of 3.21～45.02 μg·mL~(-1)(R~2=0.9999)and the average recoveries was 96.83%(RSD=1.11%)for quercetin. Conclusion The established method is accurate and reproducible,and can be applied for the determination of quercetin in Duzhong Pingya Tablet.
引文
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[7] 王学军,梁旭华,徐恒.HPLC法同时测定杜仲叶中4种成分的含量[J].中医药信息,2018,34(1):33-35.
[8] 魏薇,王建刚.HPLC测定不同树龄杜仲叶中黄酮类成分的含量[J].安徽农业科学,2011,39(18):10779-10781.
[2] 罗飞华,杨莉,张萍,等.杜仲叶中总黄酮抗氧化性能研究[J].应用化工,2016,45(5):858-859.
[3] 刘静,濮智颖,李爱玲,等.杜仲叶黄酮降血脂及抗氧化作用的研究[J].安徽农业科学,2010,38(11):5631-5632.
[4] Nishida S,Sato H.Comparative vasodilating actions among terpenoids and flavonoids contained in Ginkgobiloba extract[J].Clin Chim Acta,2004,339(1-2):129-133.
[5] Moon YJ.Recent update of renin-angiotensin-aldosterone system in the pathogenesis of hypertension[J].Electrolytes Blood Pressure,2013,11(2):41-45.
[6] 国家食品药品监督管理局.杜仲平压片质量标准[S].WS3-B-2143-96-5,2016.
[7] 王学军,梁旭华,徐恒.HPLC法同时测定杜仲叶中4种成分的含量[J].中医药信息,2018,34(1):33-35.
[8] 魏薇,王建刚.HPLC测定不同树龄杜仲叶中黄酮类成分的含量[J].安徽农业科学,2011,39(18):10779-10781.