八月瓜叶、果皮和果肉中酚类、V_C含量及其抗氧化能力分析
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摘要
为了解八月瓜的活性组分及综合抗氧化活性,以贵州省遵义县八月瓜为试材,分别测定其叶、果皮和果肉中总酚、总黄酮、抗坏血酸及9种酚类成分含量。用总还原力(total reducing assay power,TRPA)、铁离子还原能力(ferric ion reducing antioxidant power,FRAP)和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐[2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt,ABTS)]法进行体外抗氧化活性评价,采用皮尔森相关性分析对八月瓜叶、果皮和果肉中各项指标进行分析。结果显示:供试八月瓜叶、果皮和果肉中主要酚类成分为没食子酸、儿茶素、绿原酸、阿魏酸、鞣花酸、芦丁和槲皮素;活性成分含量差异显著(P<0.05),叶片中没食子酸、儿茶素、p-香豆酸、阿魏酸、鞣花酸、芦丁、槲皮素、抗坏血酸和总酚含量明显高于果皮和果肉,而果皮中绿原酸、表儿茶素和总黄酮含量相对较高,果肉中各成分含量低;八月瓜叶、果皮和果肉中抗氧化活性差异显著(P<0.05),八月瓜叶的抗氧化活性指数最高;相关性分析发现:儿茶素、阿魏酸、总酚和总黄酮对TRPA值贡献极强,阿魏酸、总酚和总黄酮对FRAP值贡献极强,儿茶素、p-香豆酸、阿魏酸、抗坏血酸和总酚对ABTS~+自由基清除能力贡献极强,说明八月瓜叶和果皮是一种具有较高开发价值的药食同源资源。
The research aimed to investigate the bioactive compounds and antioxidant activity of Akebia trifoliate in Guizhou Province,the polyphenols,total flavonoids,ascorbic acid and 9 phenolic compounds in leaves,peels and pulp were analyzed using correlation analysis. The results indicated that gallic acid,catechin,chlorogenic acid,ferulic acid,ellagic acid,rutin and quercetin in the tested leaves,peels and pulp were the major phenolic compounds.The contents of active ingredients were significantly different in the leaves,peels and pulp(P<0.05),that gallic acid,catechin,p-coumaric acid,ferulic acid,ellagic acid,rutin,quercetin,ascorbic acid and total phenols in leaves were significantly higher than those in peels and pulp,while the contents of chlorogenic acid,epicatechin and total flavonoids in peels were relatively higher,while those in pulp were the lowest. The antioxidant activity of leaves,peels and pulp was significantly different(P<0.05),especially,the antioxidant activity index of leaves was the highest in Akebia trifoliate. Relevance analysis found that:catechin,ferulic acid,polyphenols and total flavonoids has a strong contribution to the total reducing power assay(TRPA)value;the ferulic acid,polyphenols and total flavonoids had a strong contribution to the ferric ion reducing antioxidant power(FRAP)value;the catechin,p-coumaric acid,ferulic acid,ascorbic acid and polyphenols had a strong contribution to ABTS~+ free radical scavenging capacity. The leaves and peels of Akebia trifoliate are a kind of medicinal and edible homologous resources with high development value.
The research aimed to investigate the bioactive compounds and antioxidant activity of Akebia trifoliate in Guizhou Province,the polyphenols,total flavonoids,ascorbic acid and 9 phenolic compounds in leaves,peels and pulp were analyzed using correlation analysis. The results indicated that gallic acid,catechin,chlorogenic acid,ferulic acid,ellagic acid,rutin and quercetin in the tested leaves,peels and pulp were the major phenolic compounds.The contents of active ingredients were significantly different in the leaves,peels and pulp(P<0.05),that gallic acid,catechin,p-coumaric acid,ferulic acid,ellagic acid,rutin,quercetin,ascorbic acid and total phenols in leaves were significantly higher than those in peels and pulp,while the contents of chlorogenic acid,epicatechin and total flavonoids in peels were relatively higher,while those in pulp were the lowest. The antioxidant activity of leaves,peels and pulp was significantly different(P<0.05),especially,the antioxidant activity index of leaves was the highest in Akebia trifoliate. Relevance analysis found that:catechin,ferulic acid,polyphenols and total flavonoids has a strong contribution to the total reducing power assay(TRPA)value;the ferulic acid,polyphenols and total flavonoids had a strong contribution to the ferric ion reducing antioxidant power(FRAP)value;the catechin,p-coumaric acid,ferulic acid,ascorbic acid and polyphenols had a strong contribution to ABTS~+ free radical scavenging capacity. The leaves and peels of Akebia trifoliate are a kind of medicinal and edible homologous resources with high development value.
引文
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[15]李晓乐,杨瑞丽,张玉莹,等.6种热带水果多酚组成及其抗氧化活性分析[J].食品研究与开发,2018,39(5):1-7
[16]李朝阁.八月瓜酒酿造及籽油、多酚提取和抗氧化性研究[D].西安:陕西科技大学,2016:1-61
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[21]Oliveira I,Baptista P,Malheiro R,et al.Influence of strawberry tree(Arbutus unedo L.)fruit ripening stage on chemical composition and antioxidant activity[J].Food Research International,2011,44(5):1401-1407
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[23]邵显会.三叶木通总黄酮和多糖的提取纯化及生物活性研究[D].西安:陕西师范大学,2012:4-53
[24]张孟琴,张丽娜,王朝阳,等.三叶木通果皮总黄酮的提取和含量测定的研究[J].食品工业科技,2010,31(1):250-253
[25]欧阳玉祝,李雪峰,姚懿桓.离子沉淀法分离八月瓜果皮中的总多酚[J].食品科学,2014,35(16):76-79
[26]顾仁勇,杨万根,余佶.响应面优化超临界CO2萃取八月瓜幼果多酚工艺[J].食品科学,2015,36(10):76-80
[27]陆俊,罗丹,张佳琦,等.三叶木通不同部位多酚、黄酮含量及抗氧化活性比较[J].食品与机械,2016,32(8):132-135,223
[28]Li L,Yao X H,Zhong C,et al.Akebia:A potential new fruit crop in China[J].Hortscience,2010,45(1):4-10
[29]关树光,於文博,潘文君,等.三叶木通中酚醇和酚醇苷的生物活性研究[J].中国民族民间医药,2015,24(23):12-13
[30]Liu Y C,Wang H M,Zeng X H.Research progress of active compounds and pharmacological effects in Akebia trifoliata(Thunb)koidz stems[J].IOP Conference Series:Earth and Environmental Science,2018,185:1-7
[31]金银萍,曲正义,崔丽丽,等.五味子不同部位黄酮和酚酸类成分的含量测定及抗氧化活性[J].中国实验方剂学杂志,2018,24(19):79-84
[2]高慧敏,王智民.木通属药用植物研究进展[J].中国中药杂志,2006,31(1):10-14
[3]汪国龙,范玉,刘庆银,等.八月瓜果实主要营养成分含量测定[J].湖北农机化,2008(5):35
[4]张孟琴,田爱琴,林纪元,等.三叶木通果皮果胶提取工艺研究[J].食品科学,2008,29(1):154-157
[5]Godos J,Castellano S,Ray S,et al.Dietary polyphenol intake and depression:results from the mediterranean healthy eating,lifestyle and aging(MEAL)study[J].Molecules,2018,23(5):2-15
[6]Huang Y Q,Liu L.Anti-aging and anti-osteoporosis effects of green teen polyphenol in a premature aging model of Bmi-1 knockout mice[J].International Journal of Clinical and Experimental Pathology,2017,10(3):3765-3777
[7]李加兴,吴萍,吴越,等.八月瓜果皮果胶提取工艺优化及其理化特性研究[J].食品工业科技,2016,37(1):275-277,283
[8]李金光.木通属植物的化学成分研究概况[J].中国野生植物,1991(1):11-18
[9]高黎明,何仰清,魏小梅,等.木通属植物化学成分及药理活性研究进展[J].西北师范大学学报(自然科学版),2004,4(1):108-114
[10]刘桂艳,王晔,马双成,等.木通属植物木通化学成分及药理活性研究概况[J].中国药学杂志,2004(5):17-19,39
[11]Carvalho C d,Mariano L V,Negr觔o V S,et al.Phenols,flavonoids and antioxidant activity of Jatropha multifida L.collected in Pindamonhangaba,Sao Paulo State,Brazil[J].Journal of Analytical&Pharmaceutical Research,2018,7(5):581-584
[12]李杰,赵雨,普玲玲,等.生姜黄酮提取工艺优化研究[J].食品研究与开发,2018,39(22):59-65
[13]Kim M H,Ha S Y,Oh M H,et al.Anti-oxidative and anti-proliferative activity on human prostate cancer cells lines of the phenolic compounds from Corylopsis coreana Uyeki[J].Molecules,2013,18:4876-4886
[14]Chander M.Anticancer efficacy of some plant phenolics-A recent scenario[J].International Journal of Current Microbiology and Applied Sciences,2018,7(10):1746-1768
[15]李晓乐,杨瑞丽,张玉莹,等.6种热带水果多酚组成及其抗氧化活性分析[J].食品研究与开发,2018,39(5):1-7
[16]李朝阁.八月瓜酒酿造及籽油、多酚提取和抗氧化性研究[D].西安:陕西科技大学,2016:1-61
[17]Xie G f,Tan S M,Yu L.Effect of cultivar on quality of the common bean during storage[J].International Agricultural Engineering Journal,2015,24(2):69-78
[18]Dragovic-Uzelac V,Savic Z,Brala A,et al.Evaluation of phenolics content and antioxidant capacity of blueberry cultivars(Vaccinium corymbosum L.)grown in Northwest Croatia[J].Food technology and biotechnology,2010,48(2):214-221
[19]丁建海,牛浩军,刘世巍,等.分光光度法测定葫芦巴中总黄酮含量[J].广州化工,2017,45(9):125-127
[20]Todorovic V,Redovnikovic I R,Todorovic Z,et al.Polyphenols,methylxanthines,and antioxidant capacity of chocolates produced in Serbia[J].Journal of Food Composition&Analysis,2015,2543:1-7
[21]Oliveira I,Baptista P,Malheiro R,et al.Influence of strawberry tree(Arbutus unedo L.)fruit ripening stage on chemical composition and antioxidant activity[J].Food Research International,2011,44(5):1401-1407
[22]Wootton-Beard P C,Moran A,Ryan L.Stability of the total antioxidant capacity and total polyphenol content of 23 commercially available vegetable juices before and after in vitro digestion measured by FRAP,DPPH,ABTS and Folin-Ciocalteu methods[J].Food Research International,2011,44(1):217-224
[23]邵显会.三叶木通总黄酮和多糖的提取纯化及生物活性研究[D].西安:陕西师范大学,2012:4-53
[24]张孟琴,张丽娜,王朝阳,等.三叶木通果皮总黄酮的提取和含量测定的研究[J].食品工业科技,2010,31(1):250-253
[25]欧阳玉祝,李雪峰,姚懿桓.离子沉淀法分离八月瓜果皮中的总多酚[J].食品科学,2014,35(16):76-79
[26]顾仁勇,杨万根,余佶.响应面优化超临界CO2萃取八月瓜幼果多酚工艺[J].食品科学,2015,36(10):76-80
[27]陆俊,罗丹,张佳琦,等.三叶木通不同部位多酚、黄酮含量及抗氧化活性比较[J].食品与机械,2016,32(8):132-135,223
[28]Li L,Yao X H,Zhong C,et al.Akebia:A potential new fruit crop in China[J].Hortscience,2010,45(1):4-10
[29]关树光,於文博,潘文君,等.三叶木通中酚醇和酚醇苷的生物活性研究[J].中国民族民间医药,2015,24(23):12-13
[30]Liu Y C,Wang H M,Zeng X H.Research progress of active compounds and pharmacological effects in Akebia trifoliata(Thunb)koidz stems[J].IOP Conference Series:Earth and Environmental Science,2018,185:1-7
[31]金银萍,曲正义,崔丽丽,等.五味子不同部位黄酮和酚酸类成分的含量测定及抗氧化活性[J].中国实验方剂学杂志,2018,24(19):79-84