降糖宁胶囊中人参皂苷Rg1、Re的含量方法学考察
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摘要
目的:建立HPLC法测定降糖宁胶囊中人参含量的方法。方法:Packed ODS-A C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.05%磷酸溶液(100:400)为流动相;检测波长为203nm,流速为1ml/min。结果:人参皂苷Rg1、人参皂苷Re进样量均在0.4μg~1.6μg范围内进样量与峰面积呈良好线性关系(r=0.9996);方法的平均回收率:人参皂苷Rg1为98.68%,RSD为1.71%(n=9);人参皂苷Re为100.81%,2.71%(n=9)。结论:通过采用本方法对降糖宁胶囊人参中(Rg1、Re)的含量进行测定,方法准确可行,灵敏度高,能够有效控制产品质量。
0bjective:To establish the determination methods of ginsenoside Rg1, ginsenoside Re In Jiangtangning capsules by HPLC. Methods:A Packed ODS-A Cl8 column(4.6mm×250mm,5μm), Acetonitrile-0.05% phosphoric acid solution(100:400) as the mobile phase. The detection wavelength was 203nm.The flow rate was 1 ml/min. Results:The linear range of ginsenoside Rg1,ginsenoside Re were both 0.4~1.6μg(r=0.9996). The average recovery of the method: ginsenoside Rg1 was 98.68%, RSD was 171%(n=9); ginsenoside Re 100.81%, 2.71%(n=9). Conclusion: the content was determined by the method of ginsenoside Rg1, ginsenoside Re in Jiangtangning capsules, the methods are accurate, high sensitivity, and can effectively control the quality of the products.
0bjective:To establish the determination methods of ginsenoside Rg1, ginsenoside Re In Jiangtangning capsules by HPLC. Methods:A Packed ODS-A Cl8 column(4.6mm×250mm,5μm), Acetonitrile-0.05% phosphoric acid solution(100:400) as the mobile phase. The detection wavelength was 203nm.The flow rate was 1 ml/min. Results:The linear range of ginsenoside Rg1,ginsenoside Re were both 0.4~1.6μg(r=0.9996). The average recovery of the method: ginsenoside Rg1 was 98.68%, RSD was 171%(n=9); ginsenoside Re 100.81%, 2.71%(n=9). Conclusion: the content was determined by the method of ginsenoside Rg1, ginsenoside Re in Jiangtangning capsules, the methods are accurate, high sensitivity, and can effectively control the quality of the products.
引文
[1]降糖宁胶囊卫生部标准[S].标准号:WS3-B-1971-95.
[2]刘莉,张翔.降糖宁胶囊薄层鉴别方法的研究[J].黑龙江医药.2007,20(1):18-19.
[2]刘莉,张翔.降糖宁胶囊薄层鉴别方法的研究[J].黑龙江医药.2007,20(1):18-19.