归芍地黄丸中山茱萸的定性定量方法研究
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摘要
目的:建立归芍地黄丸中山茱萸的定性和定量测定方法。方法:采用TLC对归芍地黄丸中山茱萸进行定性鉴别,并采用HPLC测定山茱萸中莫诺苷、马钱苷的含量。结果:TLC色谱斑点清晰,分离度好,专属性强;莫诺苷对照品在0.010~2.620μg范围内线性关系良好(r=0.999 9),平均回收率为102.75%,RSD为1.40%(n=6)。马钱苷对照品在0.009~2.170μg范围内线性关系良好(r=0.999 9),平均回收率为103.59%,RSD为1.10%(n=6)。结论:本方法准确、简单、可行,重复性好,可有效控制该制剂中山茱萸的质量。
Objective: To establish the qualitative and quantitative methods for Corni Fructus in Guishao Dihuang pills. Methods:A TLC method was used to identify morroniside and loganin. An HPLC method was used to determine the content of morroniside and loganin in Corni Fructus. Results: The TLC spots were clear with good separation and specificity. The linear range of morroniside was within 0. 010-2. 620 μg( r = 0. 999 9) and the average recovery was 102. 75%( RSD = 1. 40%,n = 6). The linear range of loganin was within 0. 009-2. 170 μg( r = 0. 999 9),and the average recovery was 103. 59%( RSD = 1. 10%,n = 6). Conclusion: The method is accurate,simple and feasible with good repeatability,which can be used for the quality control of Corni Fructus in the preparation.
Objective: To establish the qualitative and quantitative methods for Corni Fructus in Guishao Dihuang pills. Methods:A TLC method was used to identify morroniside and loganin. An HPLC method was used to determine the content of morroniside and loganin in Corni Fructus. Results: The TLC spots were clear with good separation and specificity. The linear range of morroniside was within 0. 010-2. 620 μg( r = 0. 999 9) and the average recovery was 102. 75%( RSD = 1. 40%,n = 6). The linear range of loganin was within 0. 009-2. 170 μg( r = 0. 999 9),and the average recovery was 103. 59%( RSD = 1. 10%,n = 6). Conclusion: The method is accurate,simple and feasible with good repeatability,which can be used for the quality control of Corni Fructus in the preparation.
引文
1中国药典[S].2010年版.一部.642
2 劳凤云,李小娜,张卫国,等.山茱萸总苷中莫诺苷及马钱苷HPLC法同时测定[J].中国公共卫生,2009,25(7):889
3 李观林.山茱萸中环烯醚萜苷类的研究进展[J].亚太传统医药,2014,10(18):48-49
4 罗海涛.HPLC法测定归芍地黄丸中马钱苷含量的方法研究[J].中国民族医药杂志,2014,7(7):53-55
5 张玉洁,李中娥.杞归脉舒胶囊薄层色谱鉴别[J].中国药师,2005,8(1):79-81
6 郭巧技,肖丽和,熊英,等.降糖宁胶囊的薄层色谱鉴别[J].中国药师,2010,13(3):444-446
7 陈繁华.补肾养阴降糖丸质量标准研究[J].中国药师,2014,17(8):1297-1300
8 唐永琛,梁学政,吕建伟,等.益水生新饮颗粒的质量标准[J].中国药师,2014,17(5):737-739
9 赵平,闫润红,刘养清,等.栽培与野生山茱萸中马钱苷和莫诺苷含量分析[J].中国方剂实验杂志,2009,15(4):7-9
10 王秀文,刘养清,闫润红,等.山茱萸中莫诺苷和马钱苷的相关性研究[J].现代中药研究与实践,2009,22(6):65-67
11 李伟许,惠琴陶,玉菡,等.RP-HPLC法测定山茱萸环烯醚萜总苷中马钱苷和莫诺苷含量[J].南京中医药大学学报,2014,30(1):90-92
12 黄晓燕,李玲,李荣玮,等.高效液相色谱法同时测定归芍地黄丸中4种化学成分的含量[J].中南药学,2015,13(8):855-858
13 赵勇,王艳,郑金凤,等.藤黄健骨丸质量标准研究[J].中国药师,2013,16(1):76-79
2 劳凤云,李小娜,张卫国,等.山茱萸总苷中莫诺苷及马钱苷HPLC法同时测定[J].中国公共卫生,2009,25(7):889
3 李观林.山茱萸中环烯醚萜苷类的研究进展[J].亚太传统医药,2014,10(18):48-49
4 罗海涛.HPLC法测定归芍地黄丸中马钱苷含量的方法研究[J].中国民族医药杂志,2014,7(7):53-55
5 张玉洁,李中娥.杞归脉舒胶囊薄层色谱鉴别[J].中国药师,2005,8(1):79-81
6 郭巧技,肖丽和,熊英,等.降糖宁胶囊的薄层色谱鉴别[J].中国药师,2010,13(3):444-446
7 陈繁华.补肾养阴降糖丸质量标准研究[J].中国药师,2014,17(8):1297-1300
8 唐永琛,梁学政,吕建伟,等.益水生新饮颗粒的质量标准[J].中国药师,2014,17(5):737-739
9 赵平,闫润红,刘养清,等.栽培与野生山茱萸中马钱苷和莫诺苷含量分析[J].中国方剂实验杂志,2009,15(4):7-9
10 王秀文,刘养清,闫润红,等.山茱萸中莫诺苷和马钱苷的相关性研究[J].现代中药研究与实践,2009,22(6):65-67
11 李伟许,惠琴陶,玉菡,等.RP-HPLC法测定山茱萸环烯醚萜总苷中马钱苷和莫诺苷含量[J].南京中医药大学学报,2014,30(1):90-92
12 黄晓燕,李玲,李荣玮,等.高效液相色谱法同时测定归芍地黄丸中4种化学成分的含量[J].中南药学,2015,13(8):855-858
13 赵勇,王艳,郑金凤,等.藤黄健骨丸质量标准研究[J].中国药师,2013,16(1):76-79