洋葱多肽及蛋白对T2DM小鼠降血糖及降血脂作用研究
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摘要
目的:研究洋葱多肽及蛋白的体内降糖降脂活性。方法:将造模成功的小鼠进行糖耐量实验和药效实验,利用各生化指标参数和动物组织切片,探究洋葱多肽及蛋白的体内降糖及降脂作用。结果:200 mg/kg bw洋葱蛋白及洋葱多肽的降糖效果达到盐酸二甲双胍降糖效果的70%及80%以上;洋葱多肽组则在60、120 min时的血糖值及糖耐量AUC均显著低于模型组,分别达到阳性对照组的87.34%、93.53%和92.44%;洋葱蛋白组的AST和UREA高于正常值但低于模型组,洋葱多肽组与阳性对照组各项指标均在阈值内。结论:洋葱蛋白和洋葱多肽能有效降低2型糖尿病小鼠血糖和血脂,有效提高其口服耐糖量,并能对受损的小鼠肝、肾予以一定程度的恢复,且洋葱多肽的作用优于洋葱蛋白。
Objective: To investigate the hypoglycemic and hypolipidemic activity of the onion polypeptide and protein in vivo. Methods: A model of T2 DM mice was established by feeding with high glucose and fat diet combined with streptozocin. Glucose tolerance test and drug efficacy test were carried out on the T2 DM model mice. In addition, the biochemical indexes and the animal tissue section were utilized to explore the hypoglycemic and hypolipidemic effect of onion polypeptide and protein in vivo. Results: The hypoglycemic activity of onion protein(200 mg/kg bw) and onion polypeptide(200 mg/kg bw) reached more than 70% and 80% of metformin(400 mg/kg bw). Furthermore, the blood glucose level at 60, 120 min, and glucose tolerance of AUC of onion polypeptide group were significantly lower than that of the model group, decreased by 27.72%, 38.80% and 25.99% than that of the model group, respectively, and reached to 87.34%, 93.53% and 92.44% that of the normal group, respectively. Moreover, the level of AST and UREA of onion protein group were higher than that of the normal group, but lower than that of model group, and all biochemical indexes of onion polypeptide group and positive control group were within the threshold. Conclusion: Onion polypeptide and protein could reduce the level of blood glucose and lipid, improve the oral glucose tolerance of T2 DM mice, and recover the heart, liver and kidney damage in T2 DM mice, and the hypoglycemic and hypolipidemic effect of onion polypeptide was better than onion protein.
Objective: To investigate the hypoglycemic and hypolipidemic activity of the onion polypeptide and protein in vivo. Methods: A model of T2 DM mice was established by feeding with high glucose and fat diet combined with streptozocin. Glucose tolerance test and drug efficacy test were carried out on the T2 DM model mice. In addition, the biochemical indexes and the animal tissue section were utilized to explore the hypoglycemic and hypolipidemic effect of onion polypeptide and protein in vivo. Results: The hypoglycemic activity of onion protein(200 mg/kg bw) and onion polypeptide(200 mg/kg bw) reached more than 70% and 80% of metformin(400 mg/kg bw). Furthermore, the blood glucose level at 60, 120 min, and glucose tolerance of AUC of onion polypeptide group were significantly lower than that of the model group, decreased by 27.72%, 38.80% and 25.99% than that of the model group, respectively, and reached to 87.34%, 93.53% and 92.44% that of the normal group, respectively. Moreover, the level of AST and UREA of onion protein group were higher than that of the normal group, but lower than that of model group, and all biochemical indexes of onion polypeptide group and positive control group were within the threshold. Conclusion: Onion polypeptide and protein could reduce the level of blood glucose and lipid, improve the oral glucose tolerance of T2 DM mice, and recover the heart, liver and kidney damage in T2 DM mice, and the hypoglycemic and hypolipidemic effect of onion polypeptide was better than onion protein.
引文
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[7]Wang H X,Ng T B.Isolation of allicepin,a novel antifungal peptide from onion(Allium cepa)bulbs[J].Journal of Peptide Science An Official Publication of the European Peptide Society,2004,10(3):173-177
[8]Wetli H A,Brenneisen R,Tschudi I,et al.Aγ-glutamyl peptide isolated from onion(Allium cepa L.)by bioassay-guided fractionation inhibits resorption activity of osteoclasts[J].Journal of Agricultural and Food Chemistry,2005,53(9):3408-3414
[9]冯潍,官爱艳,王凯利,等.马尾松花粉硫酸酯化多糖对2型糖尿病小鼠血糖的影响[J].山东师范大学学报(自然科学版),2014,29(2):145-148
[10]马璇,刘琳,张蕊,等.蓝藻蛋白对2型糖尿病小鼠胰腺组织i NOS表达的影响[J].当代医学,2010,16(7):1-3
[11]黄桂红,邓航,付翔,等.鬼针草醇提物对糖尿病小鼠糖耐量及胸腺、脾脏指数的影响[J].中国实验方剂学杂志,2012,18(8):183-186
[2]Sheela CG,Kumud K,Augusti KT.Antidiabetic effects of onion and garlic sulfoxide amino acids in rats[J].Planta Medica,1995,61(4):356-357
[3]Kim J,Kim J S,Park E.Cytotoxic and antiinflammatory effects of onion peel extract on lipopolysaccharide stimulated human colon carcinoma cells[J].Food and Chemical Toxicology,2013,62:199-204
[4]Cao Y,Gu W,Zhang J,et al.Effects of chitosan,aqueous extract of ginger,onion andgarlic on quality and shelf life of stewed-pork during refrigerated storage[J].Food Chemistry,2013,4(3):1655-1660
[5]李艳鸣.醋泡两物可降血糖止腹泻[J].科普天地,2015,(12):10-10
[6]闫政宏,周博扬,华菲,等.澳洲产红葡萄酒协同洋葱降血糖作用研究[J].西北药学杂志,2014,(2):156-159
[7]Wang H X,Ng T B.Isolation of allicepin,a novel antifungal peptide from onion(Allium cepa)bulbs[J].Journal of Peptide Science An Official Publication of the European Peptide Society,2004,10(3):173-177
[8]Wetli H A,Brenneisen R,Tschudi I,et al.Aγ-glutamyl peptide isolated from onion(Allium cepa L.)by bioassay-guided fractionation inhibits resorption activity of osteoclasts[J].Journal of Agricultural and Food Chemistry,2005,53(9):3408-3414
[9]冯潍,官爱艳,王凯利,等.马尾松花粉硫酸酯化多糖对2型糖尿病小鼠血糖的影响[J].山东师范大学学报(自然科学版),2014,29(2):145-148
[10]马璇,刘琳,张蕊,等.蓝藻蛋白对2型糖尿病小鼠胰腺组织i NOS表达的影响[J].当代医学,2010,16(7):1-3
[11]黄桂红,邓航,付翔,等.鬼针草醇提物对糖尿病小鼠糖耐量及胸腺、脾脏指数的影响[J].中国实验方剂学杂志,2012,18(8):183-186